• 생약학회지
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• 제15권1호
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• pp.39-42
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• 1984

To examine stability and a separate quantitative method of a mixed preparation of lactic acid bacteria, a capsule containing Lactobacillus acidophilus, Lactobacillus bulgaricus and Streptococcus thermophilus was suspended and diluted in sterile water. After the diluted suspension was spread on three media of tryptone glucose extract agar, MRS agar and MRS-sucrose agar, their colonies appeared and were counted. The viable counts exceeded the minimum number of the three bacteria and showed that the mixed preparation was stable at least for 18 months. The results also showed that a separate quantitation of viable cells of the each strain was feasible.

• 분석과학
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• 제21권1호
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• pp.25-33
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• 2008

Organic disulfide compounds are having higher theoretical capacity than the conventional cathode material and are considered as the important storage material. Here, we are reporting the preparation of poly (2,2'-dithiodianiline) PDDA/multiwall carbon nanotubes, (MWCNTs) composites under different experimental conditions. Amine functionalized and unfunctionalized MWCNTs were independently used for the preparation of composites. Composites were prepared in the presence of cetyl trimethyl bromide (CTAB), a cationic surfactant, and also in the absence of CTAB. A physical mixture of PDTDA and MWCNTs was formed with unfunctionalized MWCNTs. Grafting of PDDA onto MWCNTs was performed by chemical oxidative polymerization of 2, 2'-dithiodianiline in the presence of amine functionalized MWCNTs. The composites of MWCNTs and PDTDA were characterized for structure, morphology and thermal properties through Fourier transform infrared spectroscopy, Fourier transform Raman spectroscopy, scanning electron microscopy and UV-visible spectroscopy. The composite materials prepared by this method are expected to find applications as electrode materials for lithium batteries.

• BMB Reports
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• 제37권3호
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• pp.351-355
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• 2004

Separate protocols are commonly used to prepare plasmid DNA, chromosomal DNA, or total RNA from E. coli cells. Various methods for the rapid preparation of plasmid DNA have been developed previously, but the preparation of the chromosomal DNA and total RNA are usually laborious. We report here a simple, fast, reliable, and cost-effective method to extract total nucleic acids from E. coli by direct lysis of the cells with phenol. Five distinct and sharp bands, which correspond to chromosomal DNA, plasmid DNA, 23S rRNA, 16S rRNA, and a mixture of small RNA, were observed when analyzing the prepared total nucleic acids on a regular 1-2% agarose gel. The simple and high-quality preparation of the total nucleic acids in a singe tube allowed us to rapidly screen the recombinant plasmid, as well as to simultaneously monitor the change of the plasmid copy number and rRNA levels during the growth of E. coli in the liquid medium.

• 여성건강간호학회지
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• 제11권1호
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• pp.38-45
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• 2005

Purpose: This study was to examine the degree of prenatal emotional status, preparation for delivery, postpartum social support, and postpartum blues and to investigate the relationship of the above variables in postpartum women. Method: The subjects were 131 postpartum women. Selection criteria were women who were 2 to 8 weeks post delivery. After Informed consent was obtained, they were asked to fill out a self-administered questionnaire. The instrument was a Postnatal Depression & Anxiety check list. Result: The mean age of the subjects was 29.3 years, and 63.4% of subjects delivered their children by vaginal birth. 18.3 percent had felt depressed during their pregnancy. The mean score of the postpartum blues was 19.2. 61.8 percent of the subjects had postpartum blues and 8.4 percent experienced postpartum depression. Monthly income was negatively correlated to postpartum blues (r=-.189, p<.05), but the emotional status during pregnancy had a significant and positive relationship. However, preparation for delivery had no significant correlation with postpartum blues. While husband support and social support had a tendency to have a negative correlation. Conclusion: Further research is needed to identify the factors that affect the emotional status during pregnancy with various scales. Also, intervention programs to increase emotional support for pregnancy and husband support are needed.

• Journal of Plant Biotechnology
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• 제45권3호
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• pp.273-278
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• 2018

We performed an analysis of the effect of postharvest treatment of rhizomes of Aconitum septentrionale Koelle with the biological preparation of Bacispecin based on a cytokinin producing strain of Paenibacillus ehimensis IB-739 on the content of alkaloid lappaconitine in rhizomes. The total alkaloid content was assayed based on exhaustive extraction method. The lappaconitine content was measured by HPLC. The efficiency of the preparation was dependant on either the concentration or intensity of the growth processes in rhizomes. Both parameters varied depending on the stage of development of plants and the amount of precipitation. In the years with normal precipitation, concentrations of Bacispecin ranging from 5 to 10 g/l were found to be most effective for increasing lappaconitine content in rhizomes of A. septentrionale. Whereas, under local drought conditions, the concentration of preparation should be less than 2 g/l.

• 한국응용과학기술학회지
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• 제20권1호
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• pp.44-50
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• 2003

We studied on the preparation and evaluation of O/W type microemulsion containing "wax, liquid paraffine and quaternary ammonium salt". And also it was obtained to stability of microemulsions by mono ethylene glycol(MEG) addition. The microemulsions were generally prepared at 96${\sim}$97$^{\circ}C$ by the phase inversion method. We used polyoxyethylene(20) sorbitan monooleate(POE(20)SMO) and distearyl dimethyl ammonium chloride(D.D.A.C.) as the emulsifiers at microemulsion preparation. From the results, we could get best condition for microemulsion preparation, in case of oil phase, montanic ester wax ; 1.1wt%, paraffine wax ; 1.1wt%, liquid paraffine ; 3.1wt%, propylene glycol ; 0.6wt% and ethylene glycol monobutyl ether ; 0.6wt%, when the ratio(wt%) of D.D.A.C. and POE(20)SMO were 2 : 3. And also we could obtained that the distributed particle size of the final microemulsions were about 8${\pm}$1.5nm and the mean particle size was 7${\pm}$0.5nm. We got following results from final microemulsions that the percent of transmittance; 96${\sim}$98% at 700nm. And the microemulsion blended with MEG of 5${\sim}$15wt% showed smaller particle size and more stable distribution than non-containing MEG.

• 대한세포병리학회지
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• 제5권2호
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• pp.79-89
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• 1994

This study was performed in order to evaluate the accuracy and the usefulness of the cytology of crush preparation in central nervous system (CNS) lesions. Forty four intraoperative biopsies were performed at the time of craniotomy including 34 benign and 10 malignant lesions. Crush preparations were prepared from tiny tissue fragments of craniotomy products. All cases were stained with toluidine blue. Intraoperative diagnoses made on cytologic examination were compared with the final paraffin section diagnoses. Comparison between the results of the cytologic and histologic findings revealed an overall diagnostic accuracy of 88.6%. This study attests to the diagnostic accuracy of cytologic examination in CNS lesions. The detailed cytologic features are described and important criteria for the cytodiagnosis of CNS lesions are discussed. This review leads us to think that cytologic examination by crush preparation in CNS lesions is considered as a simple, safe and highly diagnostically accurate method.

• Journal of Construction Engineering and Project Management
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• 제5권2호
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• pp.44-52
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• 2015

Various approaches have been used in crack preparations and each of the approaches has advantages and disadvantages. Although the routing method has been widely used and seems to be the best approach among the approaches, it is not a complete solution for crack preparation. This paper compares and evaluates a pneumatic crack cleaning device (CCD) developed by Robotics and Intelligent Construction Automation group at Georgia Tech, over existing devices. Surveys were conducted to discover factors that affect the performance of crack/joint preparation work. Then, data for such information were collected via field tests for devices such as router, heat lancer, air blower and CCD. Performed field test results and follow-up interviews demonstrated that the utilization of CCD has potential to offer improvements in productivity, safety, and maintenance cost. An analytic hierarchical procedure (AHP) and economic analyses were conducted. The AHP analysis considered three factors including safety, quality and productivity while the economic analyses examined the alternatives in various ways. The results indicated that the CCD was ranked first and second for the AHP analysis and economic analysis, respectively. In conclusion, the field tests and results revealed that the utilization of CCD achieved satisfactorily in performance, quality, safety and control, and showed that it has high potential in crack cleaning practice.

• Journal of Pharmaceutical Investigation
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• 제38권6호
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• pp.381-385
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• 2008

Isosorbide dinitrate is an oral assiatant therapy agent of angina pectoris, myocardial infarction and congestive heart failure. The objective of this study was to formulate sustained release containing isosorbide dinitrate and assess their formulation variables. Pellets were prepared by fluid bed process and consist of drug layer and membrane layer. The pellets were coated with ethylcellulose along with $5{\sim}15%$ of plasticizer such as triacetin and diethyl butylrate. In vitro evaluation study was performed by comparative dissolution test between test and reference isosorbide dinitrate preparation. We could prepare sustained pellets of isosorbide dinitrate by fluid bed process which were reduced process time and had high content. The pellet coated with 1% ethylcellulose and triacetin(l5%) had a similar dissolution behavior compare to reference isosorbide dinitrate preparation controlling initial dissolution and those of dissolution at 30 min were 17.25 and 17.09%, respectively. Difference factor and similarity factor were $0{\sim}15$ and $50{\sim}100$ and there was no significant difference in bioequivalence between formulations. It might be concluded that our sustained release pellet of isosorbide dinitrate could be an alternatively delivery system to reference drug preparation.

• Archives of Pharmacal Research
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• 제19권1호
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• pp.30-35
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• 1996

Poly(l-lactic acid)(PLLA) microspheres loaded with ampicillin sodium (AMP-Na_, .betha.-lactam antibiotic, were prepared by a w/o/w multiple emulsion-solvent evaporation method. The amounts of each component in three phases (inner water phase, organic phase, and outer water phase) wre carefully examined in the preparation of PLLA microspheres. The stirring rate, another preparation parameter, was also investigated for study on the effect of mechanical stress on the drug loading and morphology of PLLA microspheres. Most of the preparation parameters had a great influence on the drug loading, surface morphology and size distribution of PLLA microspheres. PLLA microspheres with 15.89 w/w% drug loading were subjected to the in vitro release experimet. The release of ampicillin sodium was constant at a rate of 1.68 $mug/ml/day$ per 1 mg of microspheres for 18 days initial burst effect.