• Title/Summary/Keyword: predominant

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Identifications of Predominant Bacterial Isolates from the Fermenting Kimchi Using ITS-PCR and Partial 16S rDNA Sequence Analyses

  • CHIN HWA SUP;BREIDT FRED;FLEMING H. P.;SHIN WON-CHEOL;YOON SUNG-SIK
    • Journal of Microbiology and Biotechnology
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    • v.16 no.1
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    • pp.68-76
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    • 2006
  • Despites many attempts to explore the microbial diversity in kimchi fermentation, the predominant flora remains controversial to date. In the present study, major lactic acid bacteria (LAB) were investigated in Chinese cabbage kimchi in the early phase of fermention. For the samples over pH 4.0, viable cell counts of Leuconostoc and Pediococcus were $10^6\;cfu/ml$ and below $10^2\;cfu/ml$, respectively, and 20 isolates out of 172 were subjected to a biochemical identification (API 50 CH kit) as well as molecular-typing methods including ITSPCR with a RsaI digestion and 16s rRNA gene sequence analysis for species confirmation. Seven isolates were nicely assigned to Lb. brevis, 6 to Leuconostoc spp. (2 mesenteroides, 2 citreum, I carnosum, I gasicomitatum), 4 to Weissella (3 kimchii/cibaria, 1 hanii) and 2 to other Lactobacillus spp. (1 farciminis, 1 plantarum). On the other hand, the biochemical identification data revealed 9 strains of Lb. brevis, 6 strains of Leuconostocs,2 strains of Lb. plantarum and 1 strain each of Lb. coprophilus and Lactococcus lactis. However, a single isolates, YSM 16, was not matched to the ITS-PCR database constructed in the present study. Two Lb. brevis strains by API 50 CH kit were reassigned to W kimchii/cibaria, Lb. coprophilus or W hanii, respectively, judging from the results by the above molecular typing approaches. As a whole, the identification data obtained by the biochemical test were different from those of ITS-PCR molecular method by about $63\%$ at genus-level and $42\%$ at species-level. The data by the ITS-PCR method conclusively suggest that predominant LAB species is probably heterolactic Lb. brevis, followed by W kimchii/cibaria, Leuc. mesenteroides, and Leuc. citreum, in contrast to the previous reports [3] that Leuc. mesenteroides is the only a predominant species in the early phase kimchi fermentation.

Extracting Predominant Melody from Polyphonic Music using Harmonic Structure (하모닉 구조를 이용한 다성 음악의 주요 멜로디 검출)

  • Yoon, Jea-Yul;Lee, Seok-Pil;Seo, Kyeung-Hak;Park, Ho-Chong
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.47 no.5
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    • pp.109-116
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    • 2010
  • In this paper, we propose a method for extracting predominant melody of polyphonic music based on harmonic structure. Since polyphonic music contains multiple sound sources, the process of melody detection consists of extraction of multiple fundamental frequencies and determination of predominant melody using those fundamental frequencies. Harmonic structure is an important feature parameter of monophonic signal that has spectral peaks at the integer multiples of its fundamental frequency. We extract all fundamental frequency candidates contained in the polyphonic signal by verifying the required condition of harmonic structure. Then, we combine those harmonic peaks corresponding to each extracted fundamental frequency and assign a rank to each after calculating its harmonic average energy. We finally run pitch tracking based on the rank of extracted fundamental frequency and continuity of fundamental frequency, and determine the predominant melody. We measure the performance of proposed method using ADC 2004 DB and 100 Korean pop songs in terms of MIREX 2005 evaluation metrics, and pitch accuracy of 90.42% is obtained.

Developed empirical model for simulation of time-varying frequency in earthquake ground motion

  • Yu, Ruifang;Yuan, Meiqiao;Yu, Yanxiang
    • Earthquakes and Structures
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    • v.8 no.6
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    • pp.1463-1480
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    • 2015
  • This research aims to develop an empirical model for simulation of time-varying frequency in earthquake ground motion so as to be used easily in engineering applications. Briefly, 10545 recordings of the Next Generation Attenuation (NGA) global database of accelerograms from shallow crustal earthquakes are selected and binned by magnitude, distance and site condition. Then the wavelet spectrum of each acceleration record is calculated by using one-dimensional continuous wavelet transform, and the frequencies corresponding to the maximum values of the wavelet spectrum at a series of sampling time, named predominant frequencies, are extracted to analyze the variation of frequency content of seismic ground motions in time. And the time-variation of the predominant frequencies of 178 magnitude-distance-site bins for different directions are obtained by calculating the mean square root of predominant frequencies within a bin. The exponential trigonometric function is then use to fit the data, which describes the predominant frequency of ground-motion as a function of time with model parameters given in tables for different magnitude, distance, site conditions and direction. Finally, a practical frequency-dependent amplitude envelope function is developed based on the time-varying frequency derived in this paper, which has clear statistical parameters and can emphasize the effect of low-frequency components on later seismic action. The results illustrate that the time-varying predominant frequency can preferably reflect the non-stationarity of the frequency content in earthquake ground motions and that empirical models given in this paper facilitates the simulation of ground motions.

Prognostic Significance of Cigarette Smoking in Association with Histologic Subtypes of Resected Lung Adenocarcinoma

  • Yi, Jung Hoon;Choi, Pil Jo;Jeong, Sang Seok;Bang, Jung Hee;Jeong, Jae Hwa;Cho, Joo Hyun
    • Journal of Chest Surgery
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    • v.52 no.5
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    • pp.342-352
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    • 2019
  • Background: Smokers with lung adenocarcinoma have a worse prognosis than those who have never smoked; the reasons for this are unclear. We aimed to elucidate the impact of smoking on patients' prognosis and the association between smoking and clinicopathologic factors, particularly histologic subtypes. Methods: We reviewed the records of 233 patients with pathologic stage T1-4N0-2M0 lung adenocarcinomas who underwent surgery between January 2004 and July 2015. The histologic subtypes of tumors were reassessed according to the 2015 World Health Organization classification. Results: In total, 114 patients had a history of smoking. The overall survival probabilities differed between never-smokers and ever-smokers (80.8% and 65.1%, respectively; p=0.003). In multivariate analyses, the predominant histologic subtype was an independent poor prognostic factor. Smoking history and tumor size >3 cm were independent predictors of solid or micropapillary (SOL/MIP)-predominance in the logistic regression analysis. Smoking quantity (pack-years) in patients with SOL/MIP-predominant tumors was greater than in those with lepidic-predominant tumors (p=0.000). However, there was no significant difference in smoking quantity between patients with SOL/MIP-predominant tumors and those whose tumors had non-predominant SOL/MIP components (p=0.150). Conclusion: Smoking was found to be closely associated with SOL/MIP-predominance in lung adenocarcinoma. Greater smoking quantity was related to the presence of a SOL/MIP component.

Properties of Mechanical Joint by Carbon Fiber/Epoxy Sandwich Composite Panels (탄소섬유/Epoxy 샌드위치 복합재판넬의 기계적 취부특성평가)

  • Oh, K.;Lee, S.;Jeong, J.;Cho, S.;Kim, J.
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2005.04a
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    • pp.121-124
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    • 2005
  • This paper was about experimental test properties by mechnical joint of CF1263/Epoxy Al honeycomb panels. In case of mechanical joint using screw, nut shall be secured over than minimize third screw pitch. In case of insert backsheet for increase of joint force, increase weight for assemble by screw pitch. In case of insert backsheet with CF1263/Epoxy, predominant save weight and minimazer of displacement by tensile weight moreover predominant strength. In case of mechanical joint by rivet, rivet of Monobolt has over-hole in hole of CF1263/Epoxy but rivet of PROTRUDING has predominant of mechanical joint.

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Leprous Neuropathy Associated with Predominant Proprioceptive Sensory Loss (고유감각소실을 주증상으로 보인 나병성 말초신경병증)

  • Yoon, Tae-Kwan;Jo, Hee-Young;Yuk, Ji-Won;Shin, Jin-Hong;Kim, Dae-Seong
    • Annals of Clinical Neurophysiology
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    • v.15 no.1
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    • pp.19-23
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    • 2013
  • Some patients with leprosy may present with atypical features, such as isolated peripheral neuropathy without skin lesions, or marked proprioceptive dysfunction. We report a 56-year-old female who presented with predominant proprioceptive loss without skin lesion, but was finally confirmed as leprous neuropathy by sural nerve biopsy. It is postulated that large myelinated fibers were affected by chronic immunological reactions triggered by inactive bacterial particles, producing a peripheral neuropathy presenting as predominant proprioceptive sensory loss without typical skin lesions.

Quantifying of the Persistent Periods of the Positive and Negative Sea Surface Temperature Anomalies at the Coastal Areas of the Korean Peninsula (한국연안 이상고수온과 저수온의 지속성 기간의 정량화)

  • 서영상;황재동;장이현;강용균
    • Journal of Environmental Science International
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    • v.10 no.2
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    • pp.167-171
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    • 2001
  • The magnitudes of sea surface temperature (SST) anomalies at 13 coastal stations along the Korean peninsula in the summer and winter for the past 29years (1969-1997) are more larger than those in the spring and autumn. The periods of positive SST anomalies (negative SST anomalies) longer than 1$^{\circ}C$ were 75(74.5) months in the eastern coast of Korea, 47.8(51.6) months in the southern coast of Korea and 69.5(69.8) months in the western coast of Korea during the past 348 months (1969-1997). The predominant periods of the low-pass filtered monthly SST anomalies are 3 years or 13 months, even another predominant period is 24 months. The spatial variation of SST anomalies were confined by regional seas of the Korean peninsula, such as the East Sea, the South Sea and the West Sea itself.

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Two Case Reports of Patients with Diarrhea-predominant Irritable Bowel Syndrome Treated with Jeoreongchajeonja-tang (설사형 과민성대장증후군 환자에 대한 저령차전자탕의 효과 2례)

  • Kim, Ki-tae
    • The Journal of Internal Korean Medicine
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    • v.40 no.5
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    • pp.948-956
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    • 2019
  • Objectives: The purpose of this study was to describe the effectiveness of Jeoreongchajeonja-tang as a treatment for patients with diarrhea-predominant irritable bowel syndrome (IBS-D). Methods: Two irritable bowel syndrome patients who had visited Semyung University Korean Medical Hospital were enrolled. The patients were diagnosed according to the Rome III criteria, and the treatment effect was assessed using the Gastrointestinal Symptom Rating Scale (GSRS) and the colon transit time. Results: After treatment with Jeoreongchajeonja-tang for 4 weeks, the GSRS score was improved and colon transit time was delayed. Conclusions: This study suggests that Jeoreongchajeonja-tang is effective for improving diarrhea-predominant irritable bowel syndrome. More extensive study is needed in the future.

A STUDY ON THE DEPOSITION PATTERN OF SUBGINGIVAL CALCULUS (치은연하 치석의 침착양상에 관한 연구)

  • Kang, In-Ku;Kim, Byung-Ok;Han, Kyung-Yoon
    • Journal of Periodontal and Implant Science
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    • v.24 no.1
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    • pp.1-14
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    • 1994
  • Dental calculus which is calcifing and/or calcified dental plaque is divided into supragingival calculus and subgingival calculus according to the position of deposit to gingival margin. Subgingival calculus has more important clinical significance in diagnosis and treatment of periodontal disease than supragingival calculus. In order to investigate the deposition pattern of subgingival calculus on each root surface of different tooth type, extracted 192 teeth due to excessive destruction of periodontal tissue were divided according to tooth type and the deposition pattern of subgingival calculus was classified into linear type, veneer type, scattered type, and aggregated type according to the configuration and the extent of deposit. The difference of percentage between each deposition pattern was statistically analyzed by Chi-Square test. Following results were obtained : l. In maxillary incisors, linear type and aggregated type were predominant deposition pattern of subgingival calculus on labial(45.5%, 36.4%) and palatal(36.4%, 36.4%) root surface, aggreated type(72.7%) was on mesial surface, and aggregated type(54.5%) and scattered type(36.4%) was on distal suface. 2. In mandibular incisors, scattered type, linear type and aggregated type were predominant deposition pattern of subgingival calculus on labial(33.3%, 30.6%, 27.8%) and lingual(36.1%, 30.6%, 25.0%) root surface, aggregated type(33.3%), scattered type(27.8% ), and veneer type(27.8%) were on mesial surface, and aggregated type(38.9%) and scattered type(33.3%) on distal surface. 3. In maxillary peremolars, the predominant deposition patterns of subgingival calculus were linear type(28.6%) on buccal root suface, scattered type(35.7%) and linear type(28.6%) on palatal surface, scattered type(39.3%) on mesial surface, aggregated type(46.4%) on distal surface, and aggregated type(53.6%) on furcation area. 4. In mandibular premolars, scattered type was predominant deposition pattern of subgingival calculus on buccal(39.3%) and lingual(50.0%) root surface, scattered type(32.1%) and aggregated type(32.1% ) were on mesial surface, and aggregated type(42.9%) was on distal surface. 5. In maxillary molars, aggregated type(40.0%) and scattered type(32.5%) were predominat deposition pattern of subgingival calculus on buccal root surface, aggregated type was on distal(40.0%) and furcation area(50.0%), but there was no predominat pattern on palatal and mesial root surfaces. 6. In mandibular molars, aggregated type(39.5%) and scattered type(28.9%) were predominant deposition patterns of subgingival calculus on buccal root surface, aggregated type(36.8%) was on lingual surface, linear type(39.5%) and aggregated type(34.2%) were on furcation area, but there was no predominant pattern on mesial and distal root surfaces.

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Isotyping of Immunoglobulin G Responses of Ruminants and Mice to Live and Inactivated Antigens of Cowdria ruminantium the Causative Agent of Cowdriosis in Ruminants

  • Kibor, A.C.;Sumption, K.J.;Paxton, E.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.4
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    • pp.541-548
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    • 2003
  • The Immunoglobulin $IgG_1$ and $IgG_2$ isotype immune responses of domestic ruminants and mice to Cowdria. ruminantium live infection or by immunization with inactivated organisms were determined by the enzyme linked immunosorbent assay and Western blotting. Immunization of goats with inactivated elementary bodies (IEBs) led to a predominant $IgG_1$ isotype response. This indicated that a Th2 response was induced. After challenge, the IgG isotype responses were mixed whereby both $IgG_1$ and $IgG_2$ antibodies were detected. Two goats that survived virulent challenge had a predominant $IgG_2$ isotype response. In cattle live infection by natur l challenge or experiment led to a predominant $IgG_1$ isotype response. Immunization of cattle with IEBs however led to mixed IgG responses characterized by similar $IgG_1$ and $IgG_2$ ratios. In the mouse live infection led to a predominant $IgG_2$ isotype response. This indicated the mouse developed a true Th1 type cell mediated immune response when inoculated with live organisms. Immunization with inactivated organisms on the other hand led to a dominant $IgG_1$ response. It is evident from this work that the immune responses of ruminants and mice to C. ruminantium are different and that using mice as the experimental model for immune responses to Cowdria ruminantium. is not the appropriate.