• 미생물학회지
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• 제28권1호
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• pp.6-12
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• 1990

adr 아형 B형 간염바이러스의 preS2염기서열을 베타갈락토시다세 유전자에 연결시켜 클론닝한 후, 융합단백질의 형태로 대장균에서 발현시켰다. 한국인 간염환자로부터 분리된 표면항원으로 유도한 단일 클론항체 H8는 preS2에 특이성을 지니고 있으며, 상기의 융합단백질과 특이적으로결합하였다. 이 단일 클론항체는 adw2아형 preS2 융합단백질과는 단지 낮은 수준의 결합성을 보여주었는데, 이와 같은 단일클론항체 H8의 adr 아형과 adw2아형에 대한 결합성의 차이는 preS2부위의 아미노산 차이에 기인한다고볼 수 있다.

• 미생물학회지
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• 제28권1호
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• pp.13-18
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• 1990

adr아형 B형 간염바이러스의 preS2유전자 부위를lacZ 유전자의 5말단에 연결하여 preS2-$\beta$-galactosidase 융합단백질을 생성하는 플라스미드, pTSZ를 건설하였다. 갈본된 preS2 유전자의 3' 및 5발단을 결손시켜 얻은 재조합 플라스미드를 발현시킨 후 결손된 preS2를 포함하는 융합단백질의 항원성을 단일클론항체 H8을 사용하여 비교하며 보았다. 양말단에서 일정부위까지의 결손은 항원성에 영향을 미치지 않았지만 그 이상의 결손에 의하여는 항윈성이 소실됨을 볼 수 있었다. 이상의 항원성 전한부위를 DNA 염기서열 분석에 의하여 결정할 수 있었다. 그 결과 항원결정인자의 양말단은 preS2 서열 중 아미노산 전기 130-132와 140→142 사이에 각각 존재함을 알 수 있었고, 아미노산 143번의 결손은 항원성의 부분적인 감소를 초래하는 것으로 보아 항원성 결정에 보충적 역할을 한다고 생각된다. 한편 adr과 adw2아형 간의 아미노산서열의 차이가 항원결정부위 중 130, 132 및 141번 위치에 존재하며 단일를론항체 H8이 adr아형에만 특이하게 결합하는 것으로 부터, 세 잔기 중 하나 혹은 그 이상이 아형특이성에 관여한다고 추정된다.

• Current Photovoltaic Research
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• 제10권2호
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• pp.39-48
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• 2022

A Se-containing Cu/SnSe₂/ZnSe precursor was employed to introduce S to the precursor to form Cu₂ZnSn(S,Se)₄ (CZTSSe) film. The morphology of CZTSSe films strongly varied with two different pre-annealing environments: S and N₂. The CZTSSe film with S pre-annealing showed a dense morphology with a smooth surface, while that with N₂ pre-annealing showed a porous film with a plate-shaped grains on the surface. CuS and Cu₂Sn(S,Se)₃ phases formed during the S pre-annealing stage, while SnSe and Cu₂SnSe₃ phases formed during the N₂ pre-annealing stage. The SnSe phase formed during N₂ pre-annealing generated SnS₂ phase that had plate shape and severely aggravated the morphology of CZTSSe film. The power conversion efficiency of the CZTSSe solar cell with S pre-annealing was low (1.9%) due to existence of Zn(S.Se) layer between CZTSSe and Mo substrate. The results indicated that S pre-annealing of the precursor was a promising method to achieve a good morphology for large area application.

• BMB Reports
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• 제28권4호
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• pp.353-358
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• 1995

Epitope tagging is the process of fusing a set of amino acid residues that are recognized as an antigenic determinant to a protein of interest. Tagging a protein with an epitope facilitates various immunochemical analyses of the tagged protein with a specific monoclonal antibody. The monoclonal antibody H8 has subtype specificity for an epitope derived from the preS2 region of hepatitis B virus surface antigen. Previous studies on serial deletions of the preS2 region indicated that the preS2 epitope was located in amino acid residues 130~142. To test whether the amino acid sequence in this interval is sufficient to confer on proteins the antigenicity recognizable by the antibody H8, the set of amino acid residues in the interval was tagged to the amino terminal of ${\beta}$-galactosidase and to the carboxyl terminal of the truncated $p56^{lck}$ fragment. The tagged ${\beta}$-galactosidase, expressed in Escherichia coli, maintained the enzymatic activity and was immunoprecipitated efficiently with H8. The tagged $p56^{lck}$ fragment, synthesized in an in vitro translation system, was also immunoprecipitated specifically with H8. These results demonstrate that the amino acid sequence of the preS2 region can be used efficiently for the epitope tagging approach.

• 한국과학교육학회지
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• 제31권2호
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• pp.164-180
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• 2011

본 연구에서는 초등 예비교사들에게 아동의 과학 개념을 직접 조사하고 분석하는 활동을 안내하고 수행하도록 하면서 과학 교수 학습의 원리에 대해 예비 교사들이 탐구적으로 접근할 수 있는 기회를 제공하였다. 예비교사들의 탐구 과정을 자세히 살펴보고 이 과정에서 어떠한 긍정적 학습 경험이 있었는지 알아보고자 하는 것이 이 연구의 목적이다. 연구 대상은 C 교육대학교 2개 분반 68명이며 매주 90분씩 15주간 진행되는 '초등과학교육2' 강좌에서 실시되었다. 총 20모둠으로 구성하여 모둠별 프로젝트 형식으로 탐구 활동이 진행되었으며 설문, 수업 녹화 비디오, 수업일지, 모둠의 최종 보고서, 심층 면담자료 등이 분석되었다. 데이터 분석은 다중 사례 분석 방법을 통하여 사례 내 자료 분석과 사례 간 자료 분석과정을 거쳤다. 연구 결과 예비교사의 탐구 과정에서 다음과 같은 주요한 긍정적 학습 경험이 관찰되었다. 첫 번째로 예비교사가 아동의 과학 개념 조사를 준비하는 과정은 자신의 과학 개념을 점검하고 수정, 보완하는 계기가 되었다. 두 번째로 면담 질문을 구체화하면서 예비교사의 질문 구성 능력이 향상되었다. 세 번째로 예비교사들은 아동의 과학 개념이 다양하고, 상황에 따라 불안정하다는 것을 인식하였다. 네 번째로, 예비교사들은 아동의 과학 개념에 대한 조사를 바탕으로, 구체적인 지도 방안을 도출할 수 있었다.

• 약학회지
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• 제45권6호
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• pp.708-714
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• 2001

Many studies have provided evidences that hepatitis B surface antigen (HBsAg) including preS region could be an ideal candidate for a new hepatitis B virus (HBV) vaccine with higher efficacy. We established CHO cell lines, IY-CHO-2 and IY-CHO-11 expressing high levels of HBsAg containing preS2 and S protein by stable transfection method. These cell lines expressed the correct size (about 1 kb in length) of HBsAg mRNA as expected. The purified protein from the culture supernatants of the clones showed the same sizes as those expressed in native hepatitis B virus (24 kDa, 27 kDa, 34 kDa and 36 kDa). Antibody productivity of CHO-derived HBsAg protein at lower dose challenge was higher than the protein containing S region alone expressed in yeast system. These results indicate that CHO-derived HBsAg protein containing preS2 and S region can be effectively used for a better immune response as a HBV vaccine.

• 한국보육지원학회지
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• 제16권5호
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• pp.105-126
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• 2020

Objective: This study aimed to reveal pre-service early childhood teachers' perception of children's environmental education using concept mapping and demonstrating its importance. Methods: Based on Kane and Trochim's(2007) procedure, 33 pre-service early childhood teachers conducted brainstorming and statement writing, and then the importance of selected statements were rated by 202 pre-service early childhood teachers. Selected statements were analyzed through multidimensional scaling and hierarchical cluster analysis. Results: Pre-service early childhood teachers perceived children's environmental education as concept mapping with 2-dimensions and eight clusters. The following eight clusters were established (1) daily practice through hands on experience, (2) nature environment, (3) recognizing and coping with environmental issues, (4) environmental preservation practices, (5) environmental sensitivity and consideration, (6) purpose of children's environmental education, (7) necessity for children's environmental education (8) Practice through connection with child's home and community. And then among the eight clusters, the most important was recognized as 'daily practice through hands on experience', and among the statements 'young children learn through the method of playing in nature and experience nature' was considered the most important. Conclusion/Implications: Based on these results, we discuss the importance of children's environmental education of pre-service early childhood teachers and how to support to develop teacher's training program.

• BMB Reports
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• 제29권2호
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• pp.175-179
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• 1996

Viral surface proteins are known to play an essential role in attachment of the virus particle to the host cell membrane. In case of the hepatitis B virus (HBV) several reports have described potential receptors on the target cell side, but no definite receptor protein has been isolated yet. As for the viral side, it has been suggested that the preS region of the envelope protein, especially the preS1 region, is involved in binding of HBV to the host cell. In this study, preS1 region was recombinantly expressed in the form of a maltose binding protein (MBP) fusion protein and used to identify and visualize the expression of putative HBV receptor(s) on the host cell. Using laser scanned confocal microscopy and by FACS analysis, MBP-preS1 proteins were shown to bind to the human hepatoma cell line HepG2 in a receptor-ligand specific manner. The binding kinetic of MBP-preS1 to its cellular receptor was shown to be temperature and time dependent. In cells permeabilized with Triton X-100 and treated with the fusion protein, a specific staining of the nuclear membrane could be observed. To determine the precise location of the receptor binding site within the preS1 region, several short overlapping peptides from this region were synthesized and used in a competition assay. In this way the receptor binding epitope in preS1 was revealed to be amino acid residues 27 to 51, which is in agreement with previous reports. These results confirm the significance of the preS1 region in virus attachment in general, and suggest an internalization pathway mediated by direct attachment of the viral particle to the target cell membrane.

• 한국수학교육학회지시리즈A:수학교육
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• 제57권2호
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• pp.179-196
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• 2018

The purpose of this study was to investigate the measurement equivalence and to suggest application ways in teaching aptitude and personality test results for pre-service mathematics teachers between a graduate school of education and a college of education. This study analyzed the scores of the teaching aptitude and personality test of 36 pre-service mathematics teachers enrolled in a graduate school of education and 111 pre-service mathematics teachers in a college of education by performing a multivariate generalizability analysis. The main results were as follows. First, graduate's pre-service mathematics teachers had a higher level of teaching aptitude and personality than that of college's pre-service mathematics teachers based on the total scores. In addition, graduate's pre-service mathematics teachers had higher levels of teaching aptitude and personality than those of college's pre-service mathematics teachers except for a creativity application domain based on the sub-domain scores. Second, cognitive domains were measured more precisely but affective domains were measured less precisely for graduate's pre-service mathematics teachers than for college's pre-service mathematics teachers. Third, regardless of school levels, Cronbach's ${\alpha}$ values, which might be overestimated by applying the classical test theory, were higher than dependability coefficients. Fourth, this study showed a somewhat negative result in ensuring the measurement equivalence for a problem solving exploration domain. However, regardless of school levels, this study indicated that the overall measurement was generally reliable on composite scores. Based on these results, it was confirmed that multivariate generalizability methodologies' approach can be useful for exploring the measurement equivalence issues. Finally, this study suggests how to utilize the results of the test, how to apply a multivariate generalizability analysis for detecting the measurement equivalence, and how to develop future research based on limitations.

• Animal cells and systems
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• 제11권2호
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• pp.129-134
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• 2007

The initiation of eukaryotic DNA replication requires assembly of the pre-replicative complex (Pre-RC) through the concerted action of Orc, Cdc6, Cdt1 and Mcm2-7 complex during G1 phase. The pre-RC assembly licenses individual replication origins for the initiation of DNA replication and sufficient number of the pre-RC is essential for proper progression of S phase. However, it is not well known how cells recognize the completion of the pre-RC assembly before G1-S transition. In order to understand the cellular responses to the defects in pre-RC assembly, we depleted the known components of pre-RC proteins using the small interference RNAs in HeLa cells. Although the defects of pre-RC assembly by the depletion of the pre-RC proteins such as Orc2, Cdt1, Mcm2 & Mcm10 did not elicit the activation of Chk1- or Chk2-dependent checkpoint pathways, these cells still showed significant decrease in the cellular level of Cdc25A proteins. These results suggests that a novel checkpoint pathway exist in HeLa cells, which is not dependent upon Chk1 or Chk2 proteins and play essential roles in the cellular responses to the defects in the pre-RC assembly. Also, among those four proteins tested in this study, the depletion of Mcm10 and Cdt1 proteins significantly increased the apoptotic cell death in HeLa cells, suggesting that these proteins not only play roles in the pre-RC assembly, but also are involved in the checkpoint responses to the defects in the pre-RC assembly.