BMB Reports
- Volume 28 Issue 4
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- Pages.353-358
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- 1995
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- 1976-670X(eISSN)
Epitope Tagging with a Peptide Derived from the preS2 Region of Hepatitis B Virus Surface Antigen
- Kang, Hyun-Ah (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
- Yi, Gwan-Su (Magnetic Resonance Lab, Korea Basic Science Center) ;
- Yu, Myeong-Hee (Korea Research Institute of Bioscience and Biotechnology, KIST)
- Published : 1995.07.31
Abstract
Epitope tagging is the process of fusing a set of amino acid residues that are recognized as an antigenic determinant to a protein of interest. Tagging a protein with an epitope facilitates various immunochemical analyses of the tagged protein with a specific monoclonal antibody. The monoclonal antibody H8 has subtype specificity for an epitope derived from the preS2 region of hepatitis B virus surface antigen. Previous studies on serial deletions of the preS2 region indicated that the preS2 epitope was located in amino acid residues 130~142. To test whether the amino acid sequence in this interval is sufficient to confer on proteins the antigenicity recognizable by the antibody H8, the set of amino acid residues in the interval was tagged to the amino terminal of