• Korean Journal of Environmental Agriculture
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• v.34 no.3
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• pp.223-229
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• 2015

BACKGROUND: It is important to understand residual patterns of pesticides applied on crops for ensuring their safety in agricultural products. However, there are few studies on the residual patterns of pesticides in minor crops, which are small in cultivation area. In this study, residual amounts of bifenthrin and chlorfenapyr sprayed on perilla leaf as a minor crop were investigated to know their residual patterns. METHODS AND RESULTS: Bifenthrin and chlorfenapyr were sprayed 2 or 3 times on perilla leaves at a week interval prior to harvest, and the perilla leaves were collected at 0, 1, 3, 5 and 7 days after the final application of pesticides. Recoveries for residual analysis of pesticides spiked on perilla leaves with concentrations of 0.1 and 0.5 mg/kg were 81.9-104.8%. The residual amounts of pesticides interpreted using first order kinetics model show that dissipation constants of bifenthrin and chlorfenapyr in perilla leaves were 0.0724-0.0535 and $0.0948-0.0821day^{-1}$, respectively. In addition, the dissipation half-lives in perilla leaves were 9.6-12.9 days for bifenthrin and 7.3-8.4 days for chlorfenapyr. When pre-harvest residue limits (PHRL) of bifenthrin and chlorfenapyr at 10 days before harvest calculated on the basis of the dissipation constants and maximum residue limits of the pesticides were calculated as 17.1 for bifenthrin and 15.9 mg/kg for chlorfenapyr. CONCLUSION: Therefore, the PHRL calculated using the time-dependant residual patterns of pesticides in perilla leaves and their regression analysis may be used as experimental evidences in order to ensure the safety of pesticides in perilla leaves before harvest.

• Korean Journal of Environmental Agriculture
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• v.36 no.1
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• pp.57-62
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• 2017

BACKGROUND: Pesticide residue analysis is essentially required for safety evaluation of agricultural products. Bistrifluron and chlorantraniliprole have been currently considered as potentials to deeply evaluate their residues in agricultural products because they are frequently found in strawberry. This work was performed to investigate the residual patterns of bistrifluron and chlorantraniliprole in strawberry after harvest. METHODS AND RESULTS: Strawberry was treated with bistrifluron and chlorantraniliprole 0, 1, 2, 3, 5, 7 and 10 days before harvest under greenhouse conditions. The strawberry samples were subjected to solvent and solid phase extractions followed by LC-MS/MS analysis. There covery percentages of bistrifluron and chlorantraniliprole for tified in the control samples ranged from approximately 82 to 103% with the method limit of 0.005 mg/kg. The concentrations of bistrifluron and chlorantraniliprole in strawberry samples decreased significantly in 10 days after treatment, giving the safety levels of 0.04 to 0.06 mg/kg at 10 days after application, as considered maximum residue limit. The half-lives of bistrifluron and chlorantraniliprole based on first order kinetics were determined to 6.3 days and 6.4 days, respectively. CONCLUSION: Bistrifluron and chlorantraniliprole are suggested to use in strawberry 10 days before harvest to reach residual safety levels.

• Korean Journal of Environmental Agriculture
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• v.41 no.4
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• pp.355-364
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• 2022

BACKGROUND: Pre-harvest interval and decline pattern of thiamethoxam were determined in kiwifruit using liquid chromatography-tandem mass spectrometry (LCMS/MS). The study was carried out to propose import tolerance using OECD maximum residue limit (MRL) calculator for the export promotion of kiwifruit to Taiwan. METHODS AND RESULTS: The thiamethoxam residue in kiwifruit was determined by using the LC-TriQ-MS/MS with the analytical process to set up the import tolerance under greenhouse conditions for Taiwan. Excellent linearity was observed for all of the analytes with a determination coefficient (R²)≥0.99. The limit of quantification was determined to be 0.01 mg/kg for both thiamethoxam and clothianidin in kiwifruit. Linearity was determined from the co-efficient of determinants (R²) obtained from the seven-point calibration curve. The standard calibration curve showed as follows; 1) Site 1 (Gimje): y = 944,406X + 1,583 (R²=0.9995), 2) Site 2 (Goheung): y = 1,356,205X + 934 (R²=0.9983), and 3) Site 3 (Jangheung): y = 1,239,937X - 3,090 (R²=0.9908). The residue of thiamethoxam in the kiwifruit for three decline trials showed the range of 0.35 to 0.56 mg/kg in site 1 (Gimje), 0.24 to 0.55 mg/kg in site 2 (Goheung), and 0.28 to 0.42 mg/kg in site 3 (Jangheung), respectively. However, clothianidin was not detected in all of the treatments. The maximum residual amounts (decline) in the samples, sprayed according to the safe-use standard for thiamethoxam 10% WG in kiwifruit (30 days before harvest, 3 sprays every 7 days) were 0.56 mg/kg in site 1, 0.55 mg/kg in site 2, and 0.42 mg/kg in site 3, respectively. CONCLUSION(S): The import tolerance (IT) of thiamethoxam for kiwifruit may be proposed to be 0.9 mg/kg by using the OECD MRL calculator.

• The Korean Journal of Pesticide Science
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• v.15 no.3
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• pp.269-277
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• 2011

Recent outbreak of new diseases and pests which were introduced from abroad, seriously hampered both quality and safety of paprika fruits. This study has been carried out to aid an establishment of guideline for safe use of pesticides and reduction of their residues on paprika. Systemic fungicides boscalid and pyraclostrobin of either mixed (a.i.; 13.6+6.8%) or single (a.i.; 47 and 18.8%, respectively) water dispersible granule formulation(WG) products were sprayed with recommended or double dosage on paprika grown in green house at March and June. To draw pre-harvest residue limit, residues of each fungicide were analyzed from fruits collected eight times from 18 to 1 day pre-harvest. The biological half-lives of both boscalid and pyraclostrobin in mixed formulation in March and June were slightly shorter than those of single formulation which ranged from 14.4 to 20.1 days. Residue levels of both fungicides of single formulation in fruits in June were about one lower compared to those in March. However, application of double dosage frequently exceeded MRLs from fruits grown both seasons. These results showed that residue levels on fruits persisted longer period of time, more than two weeks, and so the case applied in winter season. The dissipation of fungicides on leaves and fruits was compared. The distribution of both fungicides in leaves was 20-200 times higher than that of fruits and persisted up to 18 days of pre-harvest period at the concentration of 10-40 ${\mu}g\;g^{-1}$. This study indicated that the mixed formulation product exhibited low residues in fruits, but high and long enough to pathogen growth in leaves.

• Korean Journal of Environmental Agriculture
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• v.36 no.1
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• pp.50-56
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• 2017

BACKGROUND: The present study was carried out to identify the residue patterns of insecticide pyridalyl and fungicide fluopicolide in watermelon and calculate the biological half-lives for establishing the pre-harvest residue limits (PHRLs). METHODSANDRESULTS:The watermelon samples for residue analysis were harvested 7 times during 0~10 days (Field 1) and 0~20 days (Field 2) after treatment of pesticides on watermelon in two different fields at the recommended dose, respectively. The residue analysis was conducted with HPLC/UVD. The method limit of quantitation (MLOQ) were set at 0.05 and 0.02 mg/kg, respectively, and overall mean recoveries were 81.2~90.5% for pyridalyl and fluopicolide. The residues in sample were stable for 43~47 days. The initial residue amount in field 1 and 2 were 0.12~0.16 mg/kg for pyridalyl and 0.23~0.24 mg/kg for fluopicolide, which were below maximum residue limit (MRL). The biological half-lives in field 1 and 2 were 26.9 and 17.9 days for pyridalyl and 16.6 and 94.2 days for fluopicolide, respectively. CONCLUSION: The PHRL for watermelon were estimated as 0.21 and 1.03 mg/kg for pyridalyl and flopicolide at 10 days before harvesting. The residue patterns of pyridalyl and fluopicolide were characterized by a very slow decrease of residue levels in watermelon.

• The Korean Journal of Pesticide Science
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• v.12 no.1
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• pp.67-73
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• 2008

The dissipation patterns of a boscalid in cucumber under greenhouse condition was investigated to establish pre-harvest residue limit (PHRL) and biological half-life. Initial concentration of boscalid in cucumber at standard application rate was $7.29\;mg\;kg^{-1}$ and decreased to $0.04\;mg\;kg^{-1}$ after 15 days with half-life of 1.9 day, while the initial concentration was $14.69\;mg\;kg^{-1}$ and decreased to $0.11\;mg\;kg^{-1}$ after same period with half lift of 2.0 day at double application rate. PHRL was suggested by prediction curve derived from the decay curve of boscalid at double rate treatment. For example, $10.39\;mg\;kg^{-1}$ was calculated for 10 days before harvest, and $1.73\;mg\;kg^{-1}$ for 5 days. Dilution effect was major factor far the decrease of boscalid residue due to fast increasement of weight of cucumber during cultivation. Final residues level of boscalid was predicted based on the dissipation curve and guideline on safe use, when boscalid was used to control powdery mildew and gray mold. At standard rate application, $1.26\;mg\;kg^{-1}$ and $1.33\;mg\;kg^{-1}$ were calculated as final residue levels for control powdery mildew and gray mold, respectively, which are above the MRL(Meximum Residue Limit).

• Korean Journal of Environmental Agriculture
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• v.39 no.1
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• pp.75-82
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• 2020

BACKGROUND: This study was carried out to establish pre-harvest residue limits (PHRLs) of indoxacarb and pymetrozine in broccoli under greenhouse conditions, based on dissipation patterns and biological half-lives of pesticides during 10 days after application. METHODS AND RESULTS: The field studies were conducted in two different greenhouse, located in Chungju-si (Field 1) and Gunsan-si (Field 2). Samples were collected at 0, 1, 2, 3, 5, 7 and 10 days after spraying pesticide suspension. The analytical methods for indoxacarb and pymetrozine using HPLC-DAD were validated by recoveries ranging of 94.3-105.4% and 81.8-96.0%, respectively, and MLOQ (Method Limit of Quantification) of 0.05 mg/kg. Biological half-lives of indoxacarb and pymetrozine were 2.9 and 3.2-3.8 days in broccoli, respectively. The lower 95% confidence intervals of dissipation rate constant of indoxacarb were determined as 0.1508 (Field 1) and 0.2017 (Field 2), whereas those of pymetrozine were calculated as 0.1489 (Field 1) and 0.1577 (Field 2). CONCLUSION: The significant differences were not observed between the dissipation rates of indoxacarb and pymetrozine in broccoli. The major factor affecting residue dissipation was the dilution effect by fast growth. The PHRLs for 10 days prior to harvest were recommended as 30.06 (Field 1) and 18.07 (Field 2) mg/kg for indoxacarb, and 4.84 (Field 1) and 4.43 (Field 2) mg/kg for pymetrozine, respectively.

• Journal of Food Hygiene and Safety
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• v.34 no.2
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• pp.191-198
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• 2019

This study investigated the residual characteristics of bifenthrin and chlorothalonil in crown daisy and suggested pre-harvest residue limits (PHRLs) based on their dissipation patterns and biological half-lives. The samples for residue analysis were harvested at 0 (3 hr), 1, 3, 5, 7, 9, 11, 13, 15, 18, 22 and 26 days after treatment, and analyzed by $GC/{\mu}-ECD$ and TOF/MS. The limit of quantitation (LOQs) of bifenthrin and chlorothalonil were 0.0046 mg/kg and 0.0007 mg/kg, respectively. Recoveries ranged from $88.67{\pm}7.97%$ and $99.90{\pm}16.03%$, showing that this method is appropriate for the analysis of the pesticide residues in crown daisy. Being well within first order kinetics, the biological half-lives of the pesticide residues in crown daisy were 9.63 days for bifenthrin and 6.54 days for chlorothalonil. The PHRLs of bifenthrin and chlorothalonil were recommended as 11.70 mg/kg and 24.10 mg/kg for 26 days before harvest, respectively.

• The Korean Journal of Pesticide Science
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• v.20 no.1
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• pp.23-29
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• 2016

This study was carried out to survey residual characteristics of pesticide in fresh ginsengs collected from 45 markets at 15 regions in Korea using multiresidue analysis with a GC-MS/MS and an LC-MS/MS. After residue analysis was performed, the pesticides detected from ginsengs were quantitated using their analytical methods validated by recovery tests with a GC-ECD/NPD. As a results of analysis of pesticide residue, cypermethrin, fenitrothion, fludioxonil, thifluzamide, and tolclofos-methyl were detected from 16 samples among 45 samples in total, indicating detection rate was 35.6%. Tolclofos-methyl was found to be highest in detection frequency in ginseng. Fenitrothion that has not established maximum residue limit and pre-harvest interval for ginseng was detected. The amounts of all pesticides detected were less than their MRLs. Ratios of estimated daily intakes to acceptable daily intakes of the detected pesticides in ginseng were found to be from 0.03 to 16.67%.

• The Korean Journal of Pesticide Science
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• v.16 no.2
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• pp.131-136
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• 2012

This study was carried out to elucidate residual characteristics of azoxystrobin and difenoconazole in fresh ginseng. Test pesticides were sprayed onto ginseng both in 2009 for 3-year-old ginseng and in 2010 for 4-year-old ginseng according to their pre-harvest intervals (PHIs). Limit of quantitation (LOQ) of both azoxystrobin and difenoconazole was 0.003 mg/kg. Analytical methods set up for the test pesticides were considered to be suitable for the analysis of their residues in fresh ginseng, considering that their recoveries ranged from 87.58 to 112.79%. Concentration of azoxystrobin in 3-year-old ginseng ranged from 0.004 to 0.011 mg/kg and that in 4-year-old ginseng ranged from 0.007 to 0.016 mg/kg. Amounts of difenoconazole in 3- and 4-year-old ginsengs were from 0.003 to 0.007 and from 0.007 to 0.01 mg/kg, respectively, representing no accumulation effect observed in residue amount between them.