• Parasites, Hosts and Diseases
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• v.24 no.1
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• pp.71-76
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• 1986

Eggs of Echinestoma sp. were found from two patients who were hospitalized between Oct. and Dec. 1985. Single dose of 10 mg/kg of praziquantel was prescribed to each patient and after purgation with magnesium salt seuen and two adult worms of Echinostoma cinetorchis in respect were recovered from patients. The first case was a 54-year-old male residing at Chungju City in Chungbuk Province. He was hospitalized because of severe weight loss and frequent urination. The second case was a 71-year-old male residing at Chungsun-Kun in Kangwon Province, and hospitalized due to suspected lung cancer. The average size of the eggs found in the stools was $98{\times}63{\mu}m$ (the first case) and $100{\times}67{\mu\textrm{m}}$ (the second case) respectively, and total nine worms collected were identified as E. cinetorchis.

• Parasites, Hosts and Diseases
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• v.24 no.1
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• pp.82-88
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• 1986

Two human cases of Heterophyes heterophyes and H. dispar infections were proven by the recovery of their adult worms. The cases were 38-year and 40-year old Korean workers who had been in Saudi Arabia for 4~6 years and returned home in 1983 or 1984 with gastrointestinal troubles. In Saudi Arabia they had eaten raw brackish water fishes such as the mullet. After the treatment with 10 mg/kg praziquantel and purgation with magnesium salt, a total of 19 specimens of H. heterophyes and 140 of H. dispar were collected. It is of interest that the worms persisted in a patient although he had been back in Korea for 14 months. This is the first report on imported heterophyiasis in Korea. Human infection by H. dispar is the first record in the literature.

• Parasites, Hosts and Diseases
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• v.21 no.2
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• pp.150-156
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• 1983

Five cases of Diphyllobothrium latum infection were proved by collection of worms after treatment with bithionol, niclosamide or praziquantel, during 1975~1983. The patients were 4 males and one female aged 10~64 years residing in Seoul or Wando-gun, Chollanam-do, Korea. All of them remembered the history of eating several kinds of raw marine fish and 2 persons said they ate the raw mesh of perch, Lateolabrax japcnicus. Three amcnE them experienced abdominal pain, 2 natural discharge of a chain of segments, but none revealed any sign of anemia. The average egg size in stool varied $59~67{\times}41~48{\mu\textrm{m}}$ according to cases. The eggs were characterised by ovoid to elliptical shape, terminal operculum, and distinct abopercular protuberance. One naturally discharged segment (30cm) and 4 complete strobilae (320~680cm) with scolices obtained after treatments were examined. The morphological characters of proglottids such as rosette-form uterus with 3~6 loops, vaginal pore included in the cirrus sac, separated seminal vesicle from cirrus sac, etc. were all compatible with D. latum. These are the 7th~11th cases of D. latum infection proved by worms in Korea.

• Parasites, Hosts and Diseases
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• v.26 no.2
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• pp.121-126
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• 1988

Efficacy of praBiquantel (CesocideB injection) by intramuscular (1.M.) route against cestode infections was evaluated. Total 93 domestic or laboratory animals such as dogs, cats, rats, mice, goats, deers and chickens were used. Animals were infected with Dipylidium caninum, Spirometra sp. , Taenia pisiformis, Taenia taeniaeformis, Hymenolepis nana, Moniegia expanse, Moniexia sp. or Raillietina sp. A single dose of prasiquantel, 6 mg/kg of body weight, was highly effective (97.9%) against cestodes of various kinds disregarding the host species or their intensity of infection. At higher dose above 6 mg/kg, the cure rate was 100%. All the cestodes treated were expelled from the host within 48 hours. The discharged proglottides were damaged severely except Hymenolepis nana and Moniegia expanse. Intramuscular injection of this drug evoked a brief pain response in a dog, but no other side reactions were observed.

• Parasites, Hosts and Diseases
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• v.38 no.2
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• pp.75-84
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• 2000

Antibody responses in serum and cerebrospinal fluid (CSF) samples from patients with active and chronic paragonimiasis and in sera from patients on whom follow-up studies were done after praziquantel treatment were analyzed using antigens of Paragonimus westermani prepared from eggs, metacercariae, juveniles of 4-and 7-week old, adult worms and recombinant protein of 28 kDa cruzipain-like cysteine protease (rPw28CCP). The patient sera/CSFs of active and chronic paragonimiasis revealed strong antibody reactions against the crude extracts of 4-and 7-week old juveniles as well as against those from egg and adult. rPw28CCP also showed specific reaction to the sera with active paragonimiasis. After the treatment, levels of specific antibodies in the sera gradually decreased to negative range in most patients. In some cases with persisting high antibody levels, however, the reactions at 27 kDa egg Protein were sustained throughout the observation period of 34 months. The reactions at 35 and 32 kDa in adult extract and rPw28CCP disappeared rapidly after the treatment. Persistent antibody reactions even after successful treatment are provoked by continuous antigenic challenge from eggs which were not resolved by treatment.

• Parasites, Hosts and Diseases
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• v.32 no.1
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• pp.13-18
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• 1994

Chronic Clonorchis sinensis (CS) infection Is etiologically related to cholangiocarcinoma (CHCA) in human and animals. This study was carried out to clarify the role of CS Infection on dimethylnitrosamine (DMN)-induced cholanglocarcinogenesis. Fifteen hamsters were administered with 15 ppm DMN for 4 weeks and one week later, the hamsters were infected with 15 metacercariae of CS (DMN→CS group). The other 15 hamsters were infected with CS and after 5 weeks they were treated with the drug, praziquantel. Again one week later, the hamsters were administered with DMN (CS→DMN group). The other IS hamsters were adulnistered with DMN and CS simultaneously (CS + DMN group) . Histopathological examination of the livers showed CHCA with papillary or adenomatous hyperplasia of bile ductules in 3 of 15 hamsters of DMN→CS group and in 11 of 15 hamsters of DMN + CS group. These results suggest that CS infection to hamsters may have a promoting effect on the development of CHCA.

• Parasites, Hosts and Diseases
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• v.23 no.1
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• pp.47-58
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• 1985

An experimental study was performed to observe the in vivo effects of praziquantel on the light and scanning electron microscopic morphology of Fibricola seoulensis. The metacercariae were obtained from the snakes and 1, 000 in each number was orally given to total 15 albino rats; 5 controls and 10 treatment group. Seven days later the 10 rats were treated with 10 mg/kg praziquantcl and sacrificed 1~24 hours later to search for the worms from their small intestines. The major light microscopic changes in the drug-exposed worms were early contraction followed by relaxation of especially their forebody, vacuolization of the tegument and subtegumental parenchymal layers, and narrowing of the intestinal lumens. The scanning electron microscopic findings were characterized by formation of numerous blebs followed by rupture and subsequent destruction of their whole teguments. These results show that the change in worm body is not confined to the tegument but extends to deeper parenchymal portions and also occurs in their intestines. It is suggested that the drug uptake by the worms should be either through their tegument or through the digestive tract.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.2
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• pp.857-862
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• 2016

Background: The prevalence of infection by the liver fluke, Opisthorchis viverrini (O. viverrini), has remained high in Northeast Thailand where it is a major risk factor for the eventual development of cholangiocarcinoma (CCA). The infection is acquired by the consumption of dishes containing unsafely prepared freshwater fish, a dietary tradition which has proved resistant to change. Since many people are aware that dosing with praziquantel (PZQ) is a successful treatment for an episode of the infection, there is a risk that, to avoid the long term consequences, they will engage in a cycle of infection, dosing and reinfection. Objectives: There is a dearth of studies of reinfection by O. viverrini, and the aims of this study were to assess re-infection rates in a typical province of Northeastern Thailand where O. viverrini infection is likely and to investigate factors associated with reinfection. Materials and Methods: A total of infected 607 villagers were treated with PZQ, and those found to be no longer infected were followed up at six-monthly intervals over 12 months. Results: At the end of this period data on 457 subjects were available for analysis using descriptive statistics and logistic regression, and 50 were found to have become reinfected, giving a cumulative reinfection rate of 10.9%. The results of a multiple logistic regression analysis showed that the only factor found to be associated with reinfection was past use of PZQ. Conclusions: Recommendations are made for future larger scale and better designed reinfection studies in the light of limitations of the current study. Further efforts are needed to discourage people from eating fish dishes likely to contain viable metacariae.

• Biomedical Science Letters
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• v.2 no.2
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• pp.283-288
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• 1996

A total of 31 human diphyllobothriasis cases have been reported in Korea. Authors experienced two more human cases of Diphyllobothrium latum infection, especially due to eating raw freshwater trout. Two cases were husband and wife in a famil residing in Chunchon city, Kangwon-do, who raw fish in October 1994. A 43-year old male(husband) was 69kg in body weight and healthy. A worm (6.65m in length; 8-l3mm in width) was expelled from him after, anthelmintic treatment. The second case(wife) was a 39-year old female who weighted 56kg. She complained about gastrointestinal trouble and abdominal discomfort. A worm (5.50m in length; 9-l3mm in width) was Obtained her after anthelmintic treatment. No scolex was collected from the two worms. Two patients were administrated with praziquantel(15mg/kg of body weight) and 15gm of magnesium sulfate as a purgative. Two worms identified as D. latum, .based on the following biological characters: extermal morphologies, coiling of uterus, the number of uterine loops, position of genital opening, morphologies of cirrus and cirrus sac and seminal vesicle on the histological sections, positions of vagina and uterine pore, distribution patterns of vitellaria, and microscopical and SEM morphologies of the egg.

• Parasites, Hosts and Diseases
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• v.45 no.3
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• pp.219-223
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• 2007

A case of Diphyllobothrium latum infection in a 49-year old man is described, and diphyllobothriasis latum in the Republic of Korea is briefly reviewed. An incomplete strobila of a tapeworm, 95 cm in length, without scolex and neck, was spontaneously discharged in the feces of a patient. On the basis of morphologic characteristics of the worm and eggs, the worm was identified as D. latum. The patient was successfully treated with a single dose (15 mg/kg) of praziquantel. The most probable source of infection was salmon flesh according to the past history of the patient. The first case of D. latum infection was documented in 1971, and this is the 43rd recorded case in the Republic of Korea. The 43 cases were briefly reviewed. The patients' main complaints were gastrointestinal troubles, such as mild abdominal pain, indigestion, and diarrhea, and discharge of tapeworm segments in the feces. The suspected infection sources included raw or improperly cooked flesh of fresh or brackish water fish, including the perch, mullet, salmon, and trout.