• 제목/요약/키워드: potential efficacy

검색결과 1,171건 처리시간 0.023초

Doxorubicin에 의한 내인성 산화질소가 인간 대장암 세포주에서의 세포사멸에 미치는 효과 (Endogenous Nitric Oxide Strengthens Doxorubicin-induced Apoptosis in Human Colorectal Cell Lines)

  • 임순재;김지혜;김민영
    • 생명과학회지
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    • 제24권10호
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    • pp.1137-1143
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    • 2014
  • Doxorubicin은 광범위한 암을 치료하는데 사용되는 일반적인 항암제이지만, 내인성 산화질소 생성량과 Doxorubicin의 항암 효과의 상관 관계에 대해서는 아직 명확하게 밝혀지지 않았다. 본 연구에서는 인간 대장암 세포에서 Doxorubicin의 항암 활성에 내인성 산화질소가 미치는 영향을 확인하고자 하였다. HCT116 (p53-WT)과 HT29 (p53-MUT) 세포에서 Doxorubicin 처리에 의해 세포 생존율의 차이를 보였으며, NMA 병행처리는 Doxorubicin의 효과를 감소시켰음을 확인할 수 있었다. 추가 연구를 통해 HCT116과 HT29 세포에서 sub-$G_1$ 기의 세포 빈도와 DNA 단편화의 결과를 통해 내인성 산화질소가 Doxorubicin에 의한 apoptosis를 조절하는 것을 확인하였다. 이러한 결과는 인간 대장암 세포에서 내인성 산화질소와 IAP 발현, p53의 상태에 따른 조절이 Doxorubicin에 의해 유도된다는 것을 보여주며, 이러한 메커니즘은 대장암에서 화학요법의 효율을 향상시키기 위한 전략적인 표적으로 이용할 수 있을 것으로 생각된다.

LPS에 의해 유도된 염증반응에서 섬애약쑥 추출물의 간보호 효과 (Hepatoprotective Effects of Sumaeyaksuk (Artemisia argyi H.) Extract on LPS-mediated Inflammatory Response)

  • 김동규;강민정;신정혜
    • 생명과학회지
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    • 제26권11호
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    • pp.1282-1288
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    • 2016
  • 본 연구는 섬애약쑥의 생리활성 규명을 위한 연구의 일환으로 물 추출물의 항염증 활성 및 간 염증에 대한 보호효과를 세포수준에서 확인하였다. 세포독성이 없는 $100{\sim}400{\mu}g/ml$의 농도 범위에서 섬애약쑥 추출물은 HepG2 세포에서 nitric oxide (NO)와 reactive nitrogen species (ROS)생성을 농도의존적으로 저해하였으며, 간염질환과 밀접한 관계가 있는 cytokine인 M-CSF와 IL-8의 발현을 감소시켰다. 또한 직접적인 간 손상을 나타낼 수 있는 지표인 AST와 ALT의 발현을 유의성 있게 감소시키는 결과를 얻을 수 있었다. 본 실험을 통해서 섬애약쑥물 추출물은 세포독성이 없는 $200{\sim}400{\mu}g/ml$의 범위에서 농도의존적으로 간 염증의 개선 효과를 가질 것이라는 단서를 확인하였다.

정보시스템 개발 프로젝트 참여 만족도 결정요인에 관한 연구: SI 프로젝트 참여인력 만족도 중심으로 (A Study on Determinants of Participants' Satisfaction in Information System Development Project: Focused on Outsourcing Project Participants' Satisfaction)

  • 이주량;서길수
    • 경영정보학연구
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    • 제8권1호
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    • pp.203-222
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    • 2006
  • 아웃소싱 방식의 정보시스템 개발 프로젝트(SI 프로젝트)는 참여인력들의 만족도가 매우 낮은 것으로 알려져 있다. 이로 인해 잦은 전직과 이직, 프로젝트 참여 기피 등의 고질적인 문제가 발생하고, 프로젝트 성패에도 악영향을 미치고 있다. 참여인력들의 만족도 향상을 위해서는 프로젝트 참여만족도를 결정하는 요인들의 명확한 규명을 통한 개선이 필요하다. 프로젝트 참여만족도는 직무만족도의 일종으로 볼 수 있다. 선행연구에 의하면, 직무만족도는 직무특성과 무관한 급여, 보상, 명성 등의 일반 요인과 직무특성에 기인한 특화요인에 의하여 결정된다. 본 연구에서는 현업에서 프로젝트 참여만족도 측정에 널리 사용되는 PMI 설문에 기초하여, 정보시스템 개발 프로젝트의 특성에 기인하는 특화요인으로, 프로젝트 이행약정, 업무량과 업무시간, 프로젝트 통제, 조직내 의사소통, 조직간 의사소통, 방법론(프로세스) 영향도의 6가지 요인에 대하여 확인하고, 프로젝트 참여만족도 결정요인으로 적합한가에 대하여 66개 프로젝트에 참여한 390명의 데이터를 통해 검증했다. 연구 결과, 6개의 결정요인은 모두 프로젝트 참여만족도를 결정하는 요인으로 볼 수 있었다. 즉, 프로젝트 참여 인력들의 만족도는 프로젝트 이행약정과 프로젝트 통제, 의사소통, 프로젝트 방법론 등에 민감하게 반응하는 것으로 나타났다. 특히, 벤더 사(社) 참여인력 들의 만족도는 단순히 많은 업무량과 강한 업무강도 보다는 무리한 프로젝트 이행약정과 계획의 잦은 변경 및 불합리한 프로젝트 통제에 의하여 더욱 저해되는 것을 확인할 수 있었다. 따라서 참여자들의 프로젝트 참여 만족도를 신장시키고, 궁극적으로 프로젝트의 성과를 높이기 위해서는 단편적인 처방과 개선에서 벗어나 프로젝트 이행약정을 비롯한 프로젝트 특화요인들에 대한 종합적인 접근과 해결책이 필요할 것이다.

강황(薑黃)이 고환절제술 및 testosterone으로 유발된 rat의 전립선비대증에 미치는 영향 (The Antihyperplastic Effect of Oral Curcuma longa Ingestion in a Rat Model of Benign Prostatic Hyperplasia)

  • 이상헌;안영민;안세영;김영옥;이병철
    • 대한한방내과학회지
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    • 제30권2호
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    • pp.355-364
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    • 2009
  • Objectives : Benign prostatic hyperplasia (BPH) is one of the most common diseases among elderly men. Though medicines such as 5${\alpha}$-reductase inhibitor (finasteride) have recently been developed for treating BPH, their adverse effects and low efficacy should not be overlooked. Curcuma longa has a long history of use in traditional medicines of Asian countries. Many reports conclude the component curcumin in Curcuma lonfa, has the potential to treat various diseases including prostate cancer. In this study, we investigated the therapeutic effects and action mechanism of Curcuma longa with a BPH rat model. Methods : Sprague-Dawley rats were used with subcutaneous injection of testosterone after castration, which were histologically similar to human BPH. A total of 30 rats were equally divided into five groups: Group 1 served as control (sham-operated group): Group 2 was the model group: Group 3 and Group 4 animals were administered Curcuma longa at dose levels of 0.5g/kg and 1.0g/kg: Group 5 served as a positive control group and was treated with finasteride at a dose of 1 mg/kg. The drugs were administered orally once a day for 30 days consecutively. After 31 days, the prostates were removed, and analyzed for their prostatic weight and histological examination. Results : The oral Curcuma longa ingestion group showed statistically significant decreases in their prostatic weights compared with the BPH-induced group and the oral finasteride ingestion group (p<0.05). Curcuma longa is also very safe in liver and kidney up to a dose of lg/kg. Injected testosterone histologically led to prostatic hyperplasia in rats, but oral Curcuma longa ingestion decreased this change. Conclusions : These results suggest that Curcuma longa has a definite inhibitory effect on BPH and might be an alternative medicine for treatment and prevention of human BPH.

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박테리오파아지 CJø07의 Salmonella enteritidis 감염에 대한 SPF 병아리에서의 효능 평가 (Evaluation of Efficacy of Bacteriophage CJø07 against Salmonella enteritidis Infection in the SPF Chicks)

  • 임태현;이현정;김명섭;김병윤;양시용;송창선
    • 한국가금학회지
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    • 제37권3호
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    • pp.283-287
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    • 2010
  • 본 연구에서는 SE에 특이적인 박테리오파아지($CJ{\phi}07$)의 사료 및 음수 투여가 어린 병아리의 SE 감염에 따른 장내 균증식과 체외 균배출에 미치는 영향을 평가하고자 실시하였다. 박테리오파아지를 투여한 SPF 병아리에 SE균을 공격 접종 한 뒤, 공격 접종 10일 후 채취한 맹장 분변에 존재하는 SE균 수를 측정한 결과, 박테리오파아지를 투여한 병아리의 맹장 분변 내 SE균 수는 양성 대조군에 비하여 유의성 있게 감소하였다(P<0.05). 또한, 공격 접종 10일 후 사육 시설 표면의 SE균 오염도를 측정한 결과, 박테리오파아지를 투여한 그룹의 사육 시설에서 채취한 환경 시료의 SE균 오염율(50%)이 박테리오파아지를 투여하지 않은 그룹의 SE균 환경 오염율(100%)에 비하여 감소되는 것을 통해 박테리오파아지 $CJ{\phi}07$에 의한 장내 균 증식과 체외 균 배출 억제 효능이 확인되었다. 따라서 박테리오파아지 $CJ{\phi}07$는 항생제 대체 물질로서 가금에서의 SE 감염을 효과적으로 방어할 수 있을 것으로 판단된다.

Theileriu sergenti merozoite 수용성 항원의 항원성과 면역성 (Immunogenicity and Protective Efficacy of Solubilized Merozoite-enriched Theileria sergenti Immunogens I: Protection against Homologous Stabilate Challenge)

  • 백병걸;최인혁
    • Parasites, Hosts and Diseases
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    • 제30권2호
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    • pp.133-140
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    • 1992
  • T. sergenti merozoite 수용성 항원을 T. sergenti 감염적혈구로부터 분리하고자 저삼투압액으로 용혈, 조직 분쇄기로 분쇄한 후에 고속 원심분리하여 수용성 항원을 얻었으며, SDS-PAGE와 Western blot의 방법으로 29, 34, 35 그리고 105 kD가 함유된 항원을 본 예방접종실험의 항원성 polypeptide로 정하였다. 본 수용성 항원(0.5 mg/ml)을 준비, Freund's adjunant를 이용하여 한우(5개월령)에 경피 접종하였으며, 다시 4주 후에 추가접종하였다. 추가접종 9주 후에 예방 접종군과 대조군에 동종의 냉동충주(5.6$\times$106RBC/dose, 40% 기생률)을 접종시킨 후에 적혈구용적비, 총적혈구수, 기생률, western biot에 의한 특이항체 그리고 간접형광항체(IFA) 등을 관찰하였던 바, 예방접종 후 18주(충 접종 6주 후)에 있어서 예방접종군의 IFA는 10,240이었으나, 대조군은 1,280이었다. 예방접종군에 있어서의 충접종 전후에 있어서의 총적혈구소와 적혈구웅적비는 유의적 차이 (p<0.05)를 나타내지 않았지만, 대조군에 있어서는 적혈구용적비와 총적혈구수에서 있어서 빈혈 소견을 관찰하였다 (p<0.05). 예방접종군의 충전종 후에 있어서의 western blot 반응에서는 29, 34, 35 그리고 105 kD polypeptide의 물질이 면역반응을 잘 나타내고 있어, 이들 polypeptide는 앞으로 vaccine 제조에 활용 가능성이 충분함을 예견할 수 있었다.

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NIS quality analysis of pre- and post-harvest sugarcane.

  • Johnson, Sarah E.;Berding, Nils
    • 한국근적외분광분석학회:학술대회논문집
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    • 한국근적외분광분석학회 2001년도 NIR-2001
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    • pp.1621-1621
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    • 2001
  • The quality of sugarcane grown on the NE Australian tropical coast ($16^{\circ}$15'- $18^{\circ}$15' S Lat.) has declined markedly in the past seven years. This has been linked to dilution of mill-supply cane with increasing levels of non mature-stalk material consisting of leaves and sucker culms. The prime research objective was to examine the transition from the pre-harvest, in-field crop to harvested material sent for processing, in terms of quality and crop fraction proportions. A secondary objective was to quantify the effects of preharvest-season crop habit and culm condition on crop quality. Ten quadrat samples from each of 54 random crop sites (17 in 1999 and 37 in 2000), covering a wide range of variables (cultivar, crop class, and edaphic, topographic, climatic, and temporal factors) were collected immediately before harvest. Samples were partitioned into four fractions:- sound and unsound mature stalks (culms), sucker culms, and extraneous matter (leaves). Material harvested from each site was sampled and partitioned into four fractions:- sound and unsound billets (culm pieces), culm-spindle pieces, and leaf. In 2000, before harvest, 14 additional sites were sampled monthly, on three occasions, from March - June. Erect and non-erect culms were divided into sound and unsound classes. All samples were disintegrated and presented to a remote reflectance module of a scanning spectrophotometer using the BSES large cassette module. Near infra-red spectroscopic (NIS) analyses were developed for the rapid determination of quality components (Brix, commercial cane sugar (CCS), fibre, moisture, and polariscope reading). Calibrations for three material groups (culm (n = 639), non-culm (n = 496), and combined) were developed for all components using the 1999 data set. Two sub-sets (n = 178, and 190) of about 10% of the preharvest-season and harvest populations scanned in 2000 also were subjected to full routine laboratory analyses. The 1999 combined calibrations were excellent, but the culm calibrations produced consistently lower standard errors. Non-culm calibrations were marginally better than the combined for only CCS and pol. reading. Analysis of the 2000 culm data with calibrations using all 1999 and 2000 culm data resulted in better predictions relative to the 1999 culm calibrations. This also was true for the combined calibrations. Assessment of quality components in pre- and post-harvest sugarcane using NIS calibrations was more cost effective than using routine laboratory techniques. Outcomes from this NIS-facilitated research will have important economic consequences for the Australian sugarcane industry. Potential CCS present in mature culms is being discounted by dilution with leaves and sucker culms, threatening farm viability. The results question the efficacy of current harvesting technology. The CCS of harvested cane is improved only marginally over that of the in-field crop. Current harvesting technology requires either supplementary, innovative pre-mill processing or a design revolution to improve mill-supply cane quality, and therefore whole of industry economics. NIS-facilitated analyses, before the harvest season, highlighted the benefits of growing erect, sound crops. Loss of CCS then, can be minimized only by a combination of crop improvement and agronomic solutions, applied as part of sound on-farm management.

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Enhanced Efficacy of Human Brain-Derived Neural Stem Cells by Transplantation of Cell Aggregates in a Rat Model of Parkinson's Disease

  • Shin, Eun Sil;Hwang, Onyou;Hwang, Yu-Shik;Suh, Jun-Kyo Francis;Chun, Young Il;Jeon, Sang Ryong
    • Journal of Korean Neurosurgical Society
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    • 제56권5호
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    • pp.383-389
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    • 2014
  • Objective : Neural tissue transplantation has been a promising strategy for the treatment of Parkinson's disease (PD). However, transplantation has the disadvantages of low-cell survival and/or development of dyskinesia. Transplantation of cell aggregates has the potential to overcome these problems, because the cells can extend their axons into the host brain and establish synaptic connections with host neurons. In this present study, aggregates of human brain-derived neural stem cells (HB-NSC) were transplanted into a PD animal model and compared to previous report on transplantation of single-cell suspensions. Methods : Rats received an injection of 6-OHDA into the right medial forebrain bundle to generate the PD model and followed by injections of PBS only, or HB-NSC aggregates in PBS into the ipsilateral striatum. Behavioral tests, multitracer (2-deoxy-2-[$^{18}F$]-fluoro-D-glucose ([$^{18}F$]-FDG) and [$^{18}F$]-N-(3-fluoropropyl)-2-carbomethoxy-3-(4-iodophenyl)nortropane ([$^{18}F$]-FP-CIT) microPET scans, as well as immunohistochemical (IHC) and immunofluorescent (IF) staining were conducted to evaluate the results. Results : The stepping test showed significant improvement of contralateral forelimb control in the HB-NSC group from 6-10 weeks compared to the control group (p<0.05). [$^{18}F$]-FP-CIT microPET at 10 weeks posttransplantation demonstrated a significant increase in uptake in the HB-NSC group compared to pretransplantation (p<0.05). In IHC and IF staining, tyrosine hydroxylase and human ${\beta}2$ microglobulin (a human cell marker) positive cells were visualized at the transplant site. Conclusion : These results suggest that the HB-NSC aggregates can survive in the striatum and exert therapeutic effects in a PD model by secreting dopamine.

뇌경색 환자의 운동장애에 대한 주파수별 전침치료효과의 비교 (A Comparative Study of Motor Recovery from Stroke between High and Low Frequency Electrical Acupoint Stimulation)

  • 김영석;정우상;나병조;박성욱;문상관;박정미;고창남;조기호;배형섭;홍진우
    • 대한한의학회지
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    • 제28권3호통권71호
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    • pp.289-298
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    • 2007
  • Objectives : Electrical acupoint stimulation (EAS) has been used to treat motor dysfunction of stroke patients with reportedly effective results. The purpose of this study was to evaluate the efficacy of EAS with different frequencies in treating motor dysfunction of ischemic stroke patients. Methods : The subjects of this study were sixty-two ischemic stroke patients with motor dysfunction at Kyunghee Oriental Medicine Hospital who were hospitalized for one week to one month from onset. They were treated with 2Hz or 120Hz EAS for two weeks, and motor evoked potentials (MEP) were measured before and after EAS treatment. To compare the effect of 2Hz EAS with 120Hz, we measured latency, central motor conduction time (CMCT) and amplitude of MEP before and after EAS treatment. Results : After two weeks of treatment, we compared MEP data of the affected side between the 2Hz group and the 120Hz group. The 2Hz group showed more significant improvement than the 120Hz group in latency, CMCT and amplitude (P=0.008, 0.002, 0.002). In case of the affected side MEP data divided by normal side MEP data, the 2Hz group also showed improvement on latency, CMCT and amplitude with significant differences (P=0.003, 0.000, 0.008). Conclusions : These results suggest that low frequency EAS activates the central motor conduction system better than high frequency EAS, and it means that EAS with low frequency is more helpful for motor recovery after ischemic stroke than that with high frequency.

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Preparation and Evaluation of a 4-Branched Polyethylene Glycol Derivative Modified with Exendin-4 and Stearylamine for Extended Hypoglycemic Action

  • Kim, In-Soo;Ma, Kyung-Wan;Bae, Sung-Ho;Yoon, Jeong-Hyun;Oh, Kyung-Taek;Lee, Eun-Seong;Lee, Don-Haeng;Lee, Kang-Choon;Youn, Yu-Seok
    • Journal of Pharmaceutical Investigation
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    • 제40권3호
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    • pp.175-180
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    • 2010
  • Albumin-modification has been viewed as one of the most effective ways of extending the short in vivo lifetimes of peptide drugs by delaying glomerular filtration. In this study, we describe a new type 2 anti-diabetic exendin-4 (Ex4) peptide derivative with significant binding ability to human serum albumin (HSA). This exendin-4 derivative consists of a 4-branched polyethylene glycol $(PEG)_{5k}$ (Mw: 20 kDa) modified with three stearylamines ($C_{18}-NH_2$) and one exendin-4 on its branches. PEG and stearylamine were selected to provide functionality to increase molecular size and bind to albumin, respectively. This derivative ($3C_{18}-4PEG_{5k}$-Ex4) was shown to have larger molecular size (Ca. 152 kDa) than actual (25.0 kDa) when subjected to size-exclusion chromatography, and the fluorescein-tagged $3C_{18}-4PEG_{5k}$-Ex4 displayed significant binding to the HSA-immobilized Sepharose CL-4B resin using confocal laser scanning microscopy. Furthermore, $3C_{18}-4PEG_{5k}$-Ex4 was found to have acceptable anti-hyperglycemic efficacy via three consecutive oral glucose tolerance testings (OGTT) in fasted type 2 diabetic db/db mice. The $HD_{total}$ value ($57.6{\pm}12.3%$) of $3C_{18}-4PEG_{5k}$-Ex4 at a 50 nmol/kg dose was 2-fold greater than that ($31.0{\pm}8.7%$) of native exendin-4 in non-fasted db/db mice. Especially, the blood glucose levels in the mice group treated with $3C_{18}-4PEG_{5k}$-Ex4 did not rebound to ~150 mg/dL until 24 h after the injection, which obviously shows the extended hypoglycemia. We believe that this derivative has great pharmaceutical potential as a novel long-acting type 2 anti-diabetic injection treatment.