• Analytical Science and Technology
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• v.19 no.5
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• pp.433-440
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• 2006

A HPLC/UV method was developed and validated for the rapid and simultaneous determination of urinary metabolites of organic solvents, mandelic acid, hippuric acid, phenylglyoxylic acid, ortho-, meta- and para-methylhippuric acid, using a monolithic column. The mobile phase was composed of tetrabutylammonium bromide as ion-pairing reagent with a flow rate of 2.4 mL/min. The total run time was less than 2.5 min for all six analytes. Good linearities were obtained for all the metabolites with correlation coefficients above 0.9993. Intra-day precision, accuracy and inter-day precision was 0.01~7.32%, 83.9~116.3% and 0.01~7.16%, respectively. The method was validated and confirmed by quantification of the quality assurance samples of Industrial Safety and Health Research Institute, Korea Occupational Safety and Health Agency.

• Korean Journal of Soil Science and Fertilizer
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• v.35 no.3
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• pp.162-168
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• 2002

The silica uptake of rice plant(Oryza sativa L.) and a potential capacity of paddy soil as a source of silica supply for rice plant was studied under field experimental condition. Amount of Si from irrigation water, rainfall, which was a source of silica supply, and percolating rate of water through paddy soil profile as a source of silica loss from paddy soil, was investigated. Rice plants were grown in three different conditions, which included Control as non-fertilized, RDA's recommandation(NPK, Urea-Fused phophate-Potassium chloride=$110-45-57kg\;ha^{-1}$) and RDA's recommandation with supplement of silicate fertilizer(NPK+Si, Urea-Fused phosphate-Potassium chloride+Si=$110\;N-45\;P_2O_5-57\;K_2O+267.2\;Si\;kg\;ha^{-1}$). An amount of silica supply from rainfall was $0.5kg\;ha^{-1}$ and average amount of silica supplied from irrigation water $42.5kg\;ha^{-1}$, ranging from $28.1kg\;ha^{-1}$ to $58.8kg\;ha^{-1}$. Silica amount percolated through the soil profile have uniform trend comparatively showing $62.9kg\;ha^{-1}$ in Control, $64.8kg\;ha^{-1}$ in NPK treatment and $62.9kg\;ha^{-1}$ in NPK+Si treatment. Silica uptaked by Rice plant was $335.6kg\;ha^{-1}$ in Control, $406.6kg\;ha^{-1}$ in NPK+Si treatment and $471.1kg\;ha^{-1}$ in NPK+Si treatment. The difference between an amount of Si input(from rainfall, irrigation water and silicate fertilizer) and an amount of Si output(percolated Si in soil, uptaked Si by rice plant) was $357.4kg\;ha^{-1}$ in control, $412.1kg\;ha^{-1}$ in NPK treatment and $238.2kg\;ha^{-1}$ in NPK+Si treatment. Results of our study imply that paddy soil is a potential pool as a source of Si supply during growing periods of rice plant.

• Journal of Life Science
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• v.17 no.11
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• pp.1562-1570
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• 2007

This experiment was conducted to develop fertilizer which promotes plant growth as well as suppressing pathogenic fungi. The fertilizer was made from the mixture of Ju-Back (Korean rice wine cake) and indigenous rhizosphere-bacterium. The main ingredients of Ju-Back were investigated as 6.04% total nitrogen, 42.59% total carbohydrate, 1.01% available phosphate, 73.42% organic matter, 7.72% potassium oxide, 1.35% calcium oxide, 0.53% magnesium oxide. The enzyme activities of Ju-Back were estimated to be 980 units/g for ${\alpha}-amylase$, 300 units/g for glucoamylase, and 1800 units/g for acid pretense. Indigenous rhizosphere bacteria which produced antifungal agent were isolated from soil, and was selected KMU-13 strain which can antagonize against various plant pathogenic fungi (Botrytis cinerea KACC 40573, Sclerotinia sclerotiorum KACC 41065, Fusairum oxysporum KACC 40052, Pythium aphanidermatum KACC 40156, Phytophthora capsici KACC 40476 and Glomerella cingulata KACC 40299). KMU-13 strain was identified as Bacillus subtilis KMU-13 by biochemical and 16s rDNA analysis. The organic fertilizer was made as prototype which was composed 20% Ju-Back, 70% carrier, 9.7% microorganism cultivated solution, 0.3% trace-element. We also investigated an application of fertilizer using Ju-Back for cultivating lettuce (Lactuca sativar) which were grown in three soil conditions that had chemical fertilizer, barnyard manure, lime power, urea, potassium chloride and superphosphate as a control, the whole quantity (80 kg/10a) of posted fertilizer with the control and the half quantity (40 kg/10a) with the control. The growth characteristics were examined and analysed with several weeks interval from 3 weeks to 8 weeks on head length (cm), head width (cm/head), number of leaf and fresh weight (g/plant). The results are summarized as follows. The head width and fresh weight of lettuce were the highest at posted fertilizer 1 (whole quantity) was applied chemical, organic matter (Ju-Back) and carrier. The head length was the highest at posted fertilizer 2 (whole quantity) was applied Ju-Back only.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.6
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• pp.930-936
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• 2010

There is very important to investigate long-term trend of soil chemical properties and quality index for sustainable agriculture and production of agricultural safety products. Monitoring on soil chemical properties in paddy soils was conducted as one cycle with 4 years from 1999 to 2007. Paddy soil samples were taken from 4,007, 1,970, 2,070 sites in 1999, 2003 and 2007, respectively. With these data, soil quality index (SQI) was evaluated by method that Yoon et al suggested in 2004. Chemical properties of paddy soils were 5.8 for pH, 24 g $kg^{-1}$ for organic matter, 132 mg $kg^{-1}$ for available phosphate, 0.29 cmol_c\; kg-1 for exchangeable potassium, 4.7 $cmol_c\;kg^{-1}$ for exchangeable calcium, 1.3 $cmol_c\;kg^{-1}$ for exchangeable magnesium and 126 mg $kg^{-1}$ for available silicate in 2007. Long-term change was shown that pH has increased gradually whereas exchangeable potassium has decreased. However, reasonably large changes were found. Exchangeable calcium and available silicate level in 1999 was 4.0 $cmol_c\;kg^{-1}$, 86 mg $kg^{-1}$, but had risen to 4.7 $cmol_c\;kg^{-1}$, 126 mg $kg^{-1}$ in 2007, respectively. The change of paddy soils quality index was increased gradually and increasement of silicate quality index was higher than other quality indicators.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.8 no.1
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• pp.129-177
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• 1970

For the purpose of establishing the systematized technical scheme of the cooperative rice cultivation which has most significant impact to improve rice productivity and the farm management, the author have studied the cultivation practices, and the variation of rice growth and yield between the cooperative rice cultivation and the individual rice cultivation at random selected 18 paddy fields. The author also have investigated through comparative method on the cultivation practices, management, organization and operation scheme of the two different rice cultivation methods at 460 paddy fields. The economic feasibility has been ana lysed and added in this report. The results obtained from this study are summarized as follows; 1. In the nursery, the average amount of fertilizer application, especially, phosphate and potassium, and the frequency of chemicals spray for the disease, insect and pest control at the cooperative rice cultivation are significantly higher than those of the individual rice cultivation. 2. The cultivation techniques of the cooperative rice farming after the transplanting can be characterized by a) the earlier transplanting of rice, b) the denser hills per unit area and the lesser number of seedlings per hill, c) the application of larger quantities of fertilizer including nitrogen, phosphate and potassium, d) more divided application of fertilizers, split doses of the nitrogen and potassium, e) the increased frequencies of the chemicals spray for the prevention of disease, insect and pest damages. 3. The rate of lodging in the cooperative rice cultivation was slightly higher than that of the individual rice cultivation, however, the losses of rice yield owing to the occurrence of rice stem borer and grass leaf roller in the cooperative rice cultivation were lower than that of the individual rice cultivation. 4. The culm length, panicle length, straw weight and grain-straw ratio are respectively higher at the cooperative rice cultivation, moreover, the higher variation of the above factors due to different localities of the paddy fields found at the individual rice cultivation. 5. The number of panicles, number of flowers per panicle and the weight of 1, 000 grains, those contributing components to the rice yield were significantly greater in the cooperative rice cultivation, however, not clear difference in the maturing rate was observed. The variation coefficient of the yield component in the cooperative cultivation showed lower than that or the individual rice cultivation. 6. The average yield of brown rice per 10 are in the cooperative rice cultivation obtained 459.0 kilograms while that of the individual rice cultivation brought 374.8 kilograms. The yield of brown rice in the cooperative rice cultivation increased 84.2 kilogram per 10 are over the individual rice cultivation. With lower variation coefficient of the brown rice yield in the cooperative rice cultivation, it can be said that uniformed higher yield could be obtained through the cooperative rice cultivation. 7. Highly significant positive correlations shown between the seeding date and the number of flowers per panicle, the chemical spray and the number of flowers per panicle, the transplanting date and the number of flowers per panicle, phosphate application and yield, potassium application and maturing rate, the split application of fertilizers and yield. Whilst the significant negative correlation was shown between the transplanting date and the maturing rate 8. The results of investigation from 480 paddy fields obtained through comparative method on the following items are identical in general with those obtained at 18 paddy fields: Application of fertilizers, chemical spray for the control of disease, insects and pests both in the nursery and the paddy field, transplanting date, transplanting density, split application of fertilizers and yield n the paddy fields. a) The number of rice varieties used in the cooperative rice cultivation were 13 varieties while the individual rice cultivation used 47 varieties. b) The cooperative rice cultivation has more successfully adopted improved cultivation techniques such as the practice of seed disinfection, adoption of recommended seeding amount, fall ploughing, application of red soil, introduction of power tillers, the rectangular-type transplanting, midsummer drainage and the periodical irrigation. 9. The following results were also obtained from the same investigation and they are: a) In the cooperative rice cultivation, the greater part of the important practices have been carried out through cooperative operation including seed disinfection, ploughing, application of red soil and compost, the control of disease, insects and pests, harvest, threshing and transportation of the products. b) The labor input to the nursery bed and water control in the cooperative rice cultivation was less than that of the individual rice cultivation while the higher rate of labor input was resulted in the red soil and compost application. 10. From the investigation on the organization and operation scheme of the cooperative rice cultivation, the following results were obtained: a) The size of cooperative rice cultivation farm was varied from. 3 ha to 7 ha and 5 ha farm. occupied 55.9 percent of the total farms. And a single cooperative farm was consisted of 10 to 20 plots of paddies. b) The educational back ground of the staff members involved in the cooperative rice cultivation was superior than that of the individual rice cultivation. c) All of the farmers who participated to the questionaires have responded that the cooperative rice cultivation could promise the increased rice yield mainly through the introduction of the improved method of fertilizer application and the effective control of diseases, insects and pests damages. And the majority of farmers were also in the opinion that preparation of the materials and labor input can be timely carried out and the labor requirement for the rice cultivation possibly be saved through the cooperative rice cultivation. d) The farmers who have expressed their wishes to continue and to make further development of the cooperative rice cultivation was 74.5 percent of total farmers participated to the questionaires. 11. From the analysis of economical feasibility on the two different methods of cultivation, the following results were obtained: a) The value of operation cost for the compost, chemical fertilizers, agricultural chemicals and labor input in the cooperative rice cultivation was respectively higher by 335 won, 199 won, 288 won and 303 won over the individual rice cultivation. However, the other production costs showed no distinct differences between the two cultivation methods. b) Although the total value of expenses for the fertilizers, agricultural chemicals, labor input and etc. in the cooperative rice cultivation were approximately doubled to the amount of the individual rice cultivation, the net income, substracted operation costs from the gross income, was obtained 24, 302 won in the cooperative rice cultivation and 20, 168 won was obtained from the individual rice cultivation. Thereby, it can be said that net income from the cooperative rice cultivation increased 4, 134 won over the individual rice cultivation. It was revealed in this study that the cooperative rice cultivation has not only contributed to increment of the farm income through higher yield but also showed as an effective means to introduce highly improved cultivation techniques to the farmers. It may also be concluded, therefore, the cooperative rice cultivation shall continuously renovate the rice production process of the farmers.

• Korean Journal of Soil Science and Fertilizer
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• v.15 no.4
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• pp.258-269
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• 1982

The nutritional status of fruit leaves of sweet persimmon and soil conditions were observed in the fifty five blocks of fruit farms of sweet persimmon in Gimhae, Gyeong-nam area. 1. The soil of fruit farm was composed of more than 20% of gravel in the most part of the fruit farms. 2. The strong acidity was observed in the soil of young fruit farms but the acidity of the farm soils was improved to week acid with increase of age of fruit trees. 3. The great variation in the content of available phosphate were observed in the soil from different fruit farm blocks and the lime content were relatively low in the most of the farm soils. 4. The contents of nitrogen, phosphate and potassium in leaves of sweet persimmon collected in July and October were lower than those collected in May but reverse tendency was observed in the content of calcium, magnesium and sodium. 5. The content of boron in leaves collected in May was similar to that collected in July but extremely high content of boron was observed in the loaves collected in October, harvesting period of sweet persimmon. 6. The contents of iron and manganese in the leaves of sweet persimmon were observed to be relatively high compared to the content in the leaves of other fruit trees. 7. The relatively great variation in such inorganic components as iron, copper and boron was observed in the fruit leaves of sweet persimmon according to the different fruit farm blocks. 8. The ages of fruit trees didn't have an effect on the content of inorganic components in the fruit leaves except the content of nitrogen which was decreased with the increase of the ages of fruit trees.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.5
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• pp.744-748
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• 2012

This study was carried out to investigate the incorporation effect of green manure crops (GMC) such as the hairy vetch on improvement of soil environment in reclaimed land during sorghum${\times}$sudangrass hybrid (SSH) cultivation over the past three years from 2009 to 2011. Plots consisted of conventional fertilization (CF) and incorporation of GMC were divided by rates of additional nitrogen fertilizer ($100kg\;ha^{-1}$) and decreased percentage of 30 50 70 100 fertilization in addition to non nitrogen fertilization (NNF). Soil physico-chemical properties, growth and yield potential were examined. The results were as follows. The testing soil was showed strong alkaline saline soil with low organic matter contents and less available phosphate while exchangeable sodium and magnesium were higher. Soil salinity was increased during cultivation of summer crop. However, SSH was not affected by salt content. The fresh weight of GMC at incorporation time was $18,345kg\;ha^{-1}$. Content of total nitrogen at incorporation time was 3.09% and the C/N ratio was 12.8. Fresh and dry matter yield of SSH were higher in the order of 30%, CF, N50%, N70%d, N100%, and NNF. Fresh and dry matter yield of SSH increased in the order of CF ($55,050kg\;ha^{-1}$, $16,250kg\;ha^{-1}$), N contents from 30% to 9%. Soil physical properties, such as bulk density were decrease with incoporation of GMC, while porosity was increased. Soil chemical properties, such as pH was decreased while content of exchangeable calcium, available phosphate, and organic matter were increased. Also contents of exchangeable sodium and potassium were decreased with incorporation of GMC than those before experiment. Thus, we assumed that incorporation of hairy vetch was more effective that can lead to reduce chemical nitrogen fertilizer and to improve soil environment in cultivating SSH on Saemangeum reclaimed land.

• Korean Journal of Organic Agriculture
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• v.23 no.4
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• pp.875-886
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• 2015

The upland soils (56 samples) from organic farms in Gyeonggi-do (12 sites), Gangwon-do (8 sites), Chungcheong-do (14 sites), Gyeongsang-do (4 sites), Jeollado (18 sites) in Korea were collected and their physical and chemical properties were analyzed by RDA's methods. In the results of physical property, the bulk density of soils averaged $1.14Mgm^{-3}$ (surface soil), $1.38Mgm^{-3}$ (subsoil), respectively. The porosity of them was 57%, 48%. Organically managed soil's (OS) bulk density was lower than conventional soil's but OS's porosity was a little higher than conventionally managed soil in surface soil. The depth of plough layer in organically managed soils was 21.2 cm indicating that the organic farming had good effect on soil physical property. In the results of chemical property, the surface soil pH was 6.9 and the contents of organic matter (OM) was $26gkg^{-1}$, available phosphate (Avail. $P_2O_5$) was $554mgkg^{-1}$, exchangeable calcium (Exch. Ca) was $8.9cmol_ckg^{-1}$, exchangeable potassium (Exch. K) was $0.89cmol_ckg^{-1}$, exchangeable magnesium (Exch. Mg) was $2.0cmol_ckg^{-1}$. The subsoil pH was 6.8 and the contents of OM was $21gkg^{-1}$, avail. $P_2O_5$ was $491mgkg^{-1}$, exch. Ca was $7.9cmol_ckg^{-1}$, exch. K was $0.68cmol_ckg^{-1}$, exch. Mg was $1.8cmol_ckg^{-1}$. The nutrient accumulation emerged in organic farming. Compared to the optimum nutrient range for the conventional upland soils, the exceed rate of pH, OM, available phosphate, and exchangeable Ca, K, and Mg was 79, 52, 64, 84, 66% and 55%, respectively, which mainly resulted from the over-application of lime materials or livestock manure compost. With these results it is suggested that organic farm need to reduce the use of inputs, which make soil alkalification or nutrient accumulation. More study on effects of inputs on lowering soil pH from alkalification could help organically managed soil to be improved.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.14
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• pp.173-189
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• 1973

The followings are the results obtained in a series of experiments concerning of the varieties, the short-day-treatment, the fertilizer application, and the planting space of soybean, which were carried out to investigate the methods best suited for cultivating green soybean as the preceding crop of the rice. in the paddy-field in the middle parts of Korea at the practice farm attached to Agriculture College of Kon Kuk University in 1972. 1. Though the varieties of soybean was planted on the hot-bed on March 15 and then set in the main plot, none of them did flower within May 15, which is the limit time of flowering in growing soybean as the preceding crop of the rice in the paddy-field without the short-day-treatment being applied during raising seedlings. 2. The earliest-maturing variety groups such as HOKKAl#l, WASEMIDORI, YAEHUSANARI, MIT AKARAHAKUCHO, and VERDE flowered within May 15 by the short-day-treatment during raising seedlings. 3. The optimum hours of the day length was known to be 7 to 9 in the medium-maturing and late-maturing variety groups and 7 to 11 in the early-maturing and the earliest-maturing variety groups in the case of appling the short-day-treatment for 10 days from the beginning of the primary regular compound leaf development. 4 The optimum days in appling the short-day-treatment for 11 hours a day was recognized to be about 10 days regardless of the maturity of varieties. 5. Reduction of days required to flower by the short-clay-treatment, that is, light-sensitivity was remarkably higher in the medium-maturing and the late-maturing variety groups than in the earliest-maturing and the early-maturing variety groups. 6. The yield showed an increase of about 17 per cent in the case of appling the standard amount of nitrogen(4.0 kg/10 a), but it tended to reduce on the contrary in appling the increased amount of nitrogen. 7. The application of increased amount of phosphate had less significant effect on the yield increase than in the case of application of its standard amount( 4.0 kg/l0 a). 8. When the number of transplantation plant was changed from 54 to 130 per 3.3 $m^2$, the yield in 130 plant plot was about two times so higher than in 54 plant plot that the effect of close planting cultivation on the yield was proved to be remarkable. 9. Conditions possible for cultivating green soybean as the preceding crop of the rice in the paddy-field in the middle parts of Korea are turned to be as follows: (a) to plant the earliest-maturing-variety groups on the hot-bed on March 15. (b) to apply the short-day-treatment by 11 hours a day for 16 days from April 16, which is about the time when the primary regular leaf begin to develop. And it was, found to be a most remarkable in the increased yield that apply nitrogen 4.0 kg, phosphate 4.0 kg, and potassium 6.0 kg per 10 a as basic manuring totally and apply the close planting by 130 plants per $3.3{m^2}$(50 cm $\times$ 5 cm).

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.3
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• pp.245-252
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• 2004

Experiment was carried out to find the fertilizing effects of 8 different application rates of swine compost fermented with sawdust(SCS) including Chemical fertilizer(CF) on forage yield and soil chemical characteristics of mixed pastures sown in Sep. 1993 at National Livestock Research Institute, RDA., in Suwon during low years. It was arranged in a randomized complete block design with three replicates. Dry matter yield were shown at similar among treatments except Control and $50\%$ SCS of standard amount plot. In botanical composition, the legume and weeds percentages of each treatment were increased as advancing year. The final year's legume percentage were high in line with SCS fertilizing plots($39\%{\sim}43\%$), SCS + CF plots($30\%{\sim}41\%$) and CF plot($32\%$). In productions of TDN, NE and crude protein yield, SCS or SCS($75\%$) + CF($25\%$) were nearly same comparing those of CF, respectively. Phosphate, potassium, magnesium contents and K/(Ca + Mg) except calcium contents of those SCS fertilizing plots in plant were generally high with comparing CF. Those contents were proportional according to the fertilizing amount These result indicate the possibility to substitute chemical fertilizer for SCS($75\%$, 25ton/ha) + CF, $25\%$) as manure-N 210 kg/ha, but might be considered accumulation phosphate in the soil.