• Title/Summary/Keyword: post-synthesis

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Changes of splenocyte $IFN-{\gamma}$ mRNA synthesis in rats infected with Paragonimus westermani

  • Cho, Jun-Kyong;KWon, Hye-Soo;Joo, Kyoung-Hwan;Lee, Joon-Sang;Cho, Sung-Weon
    • Parasites, Hosts and Diseases
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    • v.37 no.4
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    • pp.285-287
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    • 1999
  • Changes in the expression level of splenocyte $IFN-{\gamma}$mRNA of Sprague-Dawley (SD) rats infected with Paragonimus westermani were analyzed by competitive reverse transcription-polymerase chain reaction (RT-PCR) followed by southern blot. The template RNA was extracted from the splenocytes of rats infected with 20 metacercariae of P. westermani. The products of competitive RT-PCR were subjected to southern blot and enhanced chemiluminescence (ECL), and analyzed with a densitometer. In comparison with that of uninfected control rat splenocytes (value of 1), the levels of mRNA expression of $IFN-{\gamma}$had changed to 0.747 at 1 week post infection (PI), 0.00175 at 2 week PI, 0.0217 at 3 week PI, 0.194 at 4 week PI and then to 0.537 at 5 week PI. The level at 7 week PI had returned to 1.25, comparable with that of uninfected rats. These results show that, when infected with p. westermani, the levels of $IFN-{\gamma}$ mRNA of SD rat splenocytes were remarkably reduced by more than 500 times at 2 week PI and restored to normal level at 7 week PI.

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REVIEW OF 15 YEARS OF HIGH-DENSITY LOW-ENRICHED UMo DISPERSION FUEL DEVELOPMENT FOR RESEARCH REACTORS IN EUROPE

  • Van Den Berghe, S.;Lemoine, P.
    • Nuclear Engineering and Technology
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    • v.46 no.2
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    • pp.125-146
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    • 2014
  • This review aims to provide a synthesis of the knowledge generated and the lessons learned in roughly 15 years of UMo dispersion fuel R&D in Europe through a series of irradiation experiments. A lot of irradiations were also performed outside of Europe, particularly in the USA, Russia, Canada, Korea and Argentina. In addition, a large number of out-of-pile investigations were done throughout the world, providing support to the understanding of the phenomena governing the UMo behaviour in pile. However, the focus of this article will be on the irradiations and Post-Irradiation Examination (PIE) results obtained in European experiments. The introduction of the article provides a historic overview of the evolution and progress in the high density UMo dispersion fuel development. The ensuing sections then provide further details on the various phases of the development, from the UMo dispersion in a pure Al matrix through the addition of Si to the matrix to address the interaction layer formation and finally to the more advanced solutions to the excessive swelling encountered in various experiments. This review was based only on published results or results that are currently in the process of being published.

Eco-Friendly Synthesis of Rod-Like Potassium Hexatitanate Particles (친환경 공정에 의한 봉상형 육티탄산칼륨 입자의 제조)

  • Lee, Chongmin;Chang, Hankwon;Jang, Hee Dong
    • Particle and aerosol research
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    • v.13 no.4
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    • pp.183-189
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    • 2017
  • Potassium hexatitanate ($K_2Ti_6O_{13}$) with high thermal insulating capacity, good mechanical properties, and excellent chemical stability are promising functional materials in the field of reinforcing material, heat insulating paints and automotive brake linings. In this study, we successfully synthesized rod-shaped potassium hexatitanate ($K_2Ti_6O_{13}$) by aerosol spray drying and post heat treatment as an eco-friendly process. The $KHCO_3-TiO_2$ porous particles were firstly synthesized from a colloidal mixture of $K_2CO_3$ and $TiO_2$ via aerosol spray drying. Size of $KHCO_3-TiO_2$ porous particles was ranged from $1{\mu}m$ to $5{\mu}m$. The porous particles were then heated to fabricate rod-type $K_2Ti_6O_{13}$. The length and width of rod-type composites were affected by temperature and heating time. The length and width of $K_2Ti_6O_{13}$ were increased by 830 nm and 500 nm, respectively, as the reaction temperature and time increased.

Depth Video Post-processing for Immersive Teleconference (원격 영상회의 시스템을 위한 깊이 영상 후처리 기술)

  • Lee, Sang-Beom;Yang, Seung-Jun;Ho, Yo-Sung
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.37 no.6A
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    • pp.497-502
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    • 2012
  • In this paper, we present an immersive videoconferencing system that enables gaze correction between users in the internet protocol TV (IPTV) environment. The proposed system synthesizes the gaze corrected images using the depth estimation and the virtual view synthesis algorithms as one of the most important techniques of 3D video system. The conventional processes, however, causes several problems, especially temporal inconsistency of a depth video. This problem leads to flickering artifacts discomforting viewers. Therefore, in order to reduce the temporal inconsistency problem, we exploit the joint bilateral filter which is extended to the temporal domain. In addition, we apply an outlier reduction operation in the temporal domain. From experimental results, we have verified that the proposed system is sufficient to generate the natural gaze-corrected image and realize immersive videoconferencing.

Prospect of plant molecular cytogenetics in the 21st century

  • Mukai, Yasuhiko
    • Proceedings of the Korean Society of Life Science Conference
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    • 2003.10a
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    • pp.14-27
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    • 2003
  • The genomes of Arabidopsis and rice have been fully sequenced. Genomic sequencing provides global information about genome structure and organization. A comprehensive research account of our recent studies conducted on genome painting, comparative genomics and genome fusion is provided in order to project the prospects of plant cytogenetic research in post-genomics era. Genome analysis by GISH using genome painting is demonstrated as an excellent means suitable for visualization of a whole genome, since total genomic DNA representing the overall molecular composition of the genome is used as a probe. FISH on extended DNA fibers has been developed for high-resolution FISH and has contributed to determining the copy number and order of genes. We have also mapped a number of genes involving starch synthesis on wheat chromosomes by FISH and compared the position of these genes on linkage map of rice. Macro synteny between wheat and rice can be observed by comparing the location of these genes in spite of the fact that the size of DNA per chromosome differs by 20 fold in two. Moreover, to approach our goal towards making bread and udon noodles from rice flour in future by incorporating bread making and the noodle qualifies in rice, we have been successful in introducing large genomic DNA fragments containing agronomically important genes of wheat into a rice by successive introduction of large insert BAC clones, there by expanding genetic variability in rice. We call this method genome fusion.

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A Novel UV-Sensitivity Mutation Induces Nucleotide Excision Repair Phenotype and Shows Epistatic Relationships with UvsF and UvsB Groups in Aspergillus nidulans

  • Baptista, F.;Castro-Prado, M.A.A.
    • Journal of Microbiology
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    • v.39 no.2
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    • pp.102-108
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    • 2001
  • DNA damage response has a central role in the maintenance of genomic integrity while mutations in related genes may result in a range of disorders including neoplasic formations. The uvsZl characterized in this report is a navel uvs mutation in Aspergillus nidulans, resulting in a nucleotide excision repair (NER) phenotype: UV-sensitivity before DNA synthesis (quiescent cells), high UV-induced mutation frequency and probable absence of involvement with mitotic and meiotic recombinations. The mutation is recessive and nan-allelic to the previously characterized uvsA101 mutation, also located on the paba-y interval on chromosome I. uvsZl skewed wild-type sensitivity to MMS, which suggests non-involvement of this mutation with BER. Epitasis tests showed that the uvsZ gene product is probably involved in the same repair pathways as UVSB or UVSH proteins. Although mutations in these proteins result in an NER phenotype, UVSB is related with cell cycle control and UVSH is associated with the post-replicational repair pathway. The epistatic interaction among uvsZl and uvsB413 and uvsH77 mutations indicates that different repair systems may be related with the common steps of DNA damage response in Aspergillus nidulans.

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Determination of Development Strategy for a Pepper Harvester (고추수확기의 개발방향 설정)

  • 이종호;박승제;김철수;이중용;김명호;김용현
    • Journal of Biosystems Engineering
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    • v.20 no.1
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    • pp.22-35
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    • 1995
  • Pepper is the most important horticultural plant in Korean farm. Pepper harvesting has been known to be the most difficult process in pepper cultivation so that demand for mechanization is strong. In a research to develop a pepper harvesting machine performance and capacity of the harvester should be determined based on both economical feasibility and machine design concept. In order to accomplish an economical analysis of the pepper harvester, a mathematical model for comparing manual harvesting cost to machine harvest cost was developed. Validity of the model depends on the data used in the model. Economical information for the model variables was acquired from the result of farm survey on pepper cultivation technique and economics of pepper farmer. Technical information on pepper harvester were also collected through literature review and analyzed. Based on the economical analysis and synthesis of the technical information on pepper harvesters, its performance and capacity were determined. The operating performances of the harvester such as cutting, conveying, flipping, pepper removing and post-processing (sorting) were determined. Daisy capacity of the machine was determined to be 0.41 ha. A pepper harvester with the suggested capacity was economically feasible if the price of pepper harvester, pepper recovery ratio and service life of harvester were about 6 million won, 80%, and 4 years, respectively.

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Synthesis of Mixed Phase Vanadium Oxides Thin Films and Their Ethanol Gas Sensing Properties (혼합 상의 바나듐 산화물 박막 제작 및 에탄올 가스 감지 특성 연구)

  • Han, Soo Deok;Kang, Chong-Yun
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.31 no.1
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    • pp.29-33
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    • 2018
  • Using a vanadium dioxide ($VO_2$) source, highly pure and amorphous vanadium oxide (VO) thin films were deposited using an e-beam evaporator at room temperature and high vacuum (<$10^{-7}$ Torr). Then, by controlling the post-annealing conditions such as $N_2:O_2$ pressure ratio and annealing time, we could easily synthesize a homogeneous $VO_2$ thin film and also mixed-phase VO thin films, including $VO_2$, $V_2O_5$, $V_3O_7$, $V_5O_9$, and $V_6O_{13}$. The crystallinity and phase of these were characterized by X-ray diffraction, and the surface morphology by FE-SEM. Moreover, the electrical properties and ethanol sensing measurements of the VO thin films were analyzed as a function of temperature. In general, mixed-phases as a self-doping effect have enhanced electrical properties, with a high carrier density and an enhanced response to ethanol. In summary, we developed an easy, scalable, and reproducible fabrication process for VO thin films that is a promising candidate for many potential electrical and optical applications.

The Synthesis of (Y,Gd)$BO_3:Eu^{3+}$Phosphor by Ultrasonic Spray and Their Photoluminance Properties (초음파 분무에 의한 (Y,Gd)$BO_3:Eu^{3+}$ 형광체의 제조와 이의 발광 특성)

  • 김대수;김성우;이임렬
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1999.11a
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    • pp.107-110
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    • 1999
  • The ultrasonic spray method was employed to make (Y,Gd)BO$_3$:Eu Phosphor, and its optical properties under 147nm VUV and 254 nm UV excitations were characterized and then compared with that produced by the solid-state reaction. The mixed solution of acetate hydrates of Y, Gd, Eu and boric acid diluted in water or methanol was used as the precursor fur the spray. It was found that (Y,Gd)BO$_3$:Eu phosphor made by this ultrasonic spray had a spherical shape and fine particle size of 1${\mu}{\textrm}{m}$. The crystalline structure for the as-sprayed phosphor was amorphous, but it converted into the same polycrystalline phase of solid state reaction after post heat treatment at 110$0^{\circ}C$ for 2hr. The emitting intensity under VUV and UV excitations for the spray-formed (Y,Gd)BO$_3$:Eu phosphor, however, was inferior to the later one. The excitation spectra were also studied and compared under VUV and UV excitations to explain the change of emitting intensity with Gd substitution in (Y$_{1-x}$ Gd$_{x}$)BO$_3$:Eu Phosphors made by spray and solid state reaction.on.

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Mixing Pyroligneous Acids with Herbicides to Control Barnyardgrass (Echinochloa crus-galli)

  • Acenas, Xernan Sebastian;Nunez, John Paolo Panisales;Seo, Pil Dae;Ultra, Venecio Uy Jr.;Lee, Sang Chul
    • Weed & Turfgrass Science
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    • v.2 no.2
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    • pp.164-169
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    • 2013
  • Alternatives to commercial chemical herbicide are currently being searched and tested due to the numerous adverse effects of commercially available herbicides to the environment. Barnyardgrass (Echinochloa crusgalli) is an important weed species around the world, especially in paddy rice fields. This study focuses on the favorable effects of mixing pyroligneous acids with commercial liquid herbicides. Seedlings were transplanted and grown under greenhouse conditions. The effect of treatment time or leaf-stage on herbicide-pyroligneous acid efficacies was checked, coupled with isolation and quantification of biochemical compounds. Results revealed that herbicide treatment at early post emergence (2~3 leaf stage) of Echnochloa crus-galli leads to effective control. Both liquid herbicides affected fatty acid, protein, and amino acid syntheses as reflected on their contents. The influence of wood vinegar (WV) or rice vinegar (RV) on these compounds was not thoroughly verified due to lack of information on the pyroligneous products. We observed that mixing WV or RV with BCB (bentazone + cyhalof-butyl) gives more favorable results than BUC (butachlor + clomazone), mixed with WV or RV. The result would indicate the potential of mixing pyroligneous acid in reducing herbicide application rate.