• The Korean Journal of Food And Nutrition
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• v.33 no.3
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• pp.309-316
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• 2020

In this study, we investigated the protective effects of Ulva lactuca methanol extracts against ultraviolet B (UVB)-induced DNA damage in HaCaT cells. First, the contents of general and antioxidative nutrient contents of Ulva lactuca were measured. The moisture, carbohydrate, crude protein, crude fat and ash were 14.01%, 44.80%, 23.19%, 3.10% and 14.90%, respectively. Magnesium that acts as DNA repair enzyme cofactor was the most abundant mineral followed by Ca, P and Fe. The total phenolic and anthocyanoside contents of Ulva lactuca were 2.69 mg/g and 0.13 mg/g, respectively. Cells treated with Ulva lactuca methanol extracts for 24 hours post UVB exposure increased cell viability in a concentration-dependent manner compared to the non-treated control. Also, Ulva lactuca methanol extracts decreased the levels of UVB-induced DNA damage such as cyclobutane pyrimidine dimer and DNA damage response (DDR) proteins such as p-p53 and p21. These results suggest that Ulva lactuca methanol extracts comprising physiological active substances such as Mg, polyphenols and anthocyanosides promote DNA repair by regulating genes related with DDR.

• Steel and Composite Structures
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• v.17 no.2
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• pp.199-213
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• 2014

The damage characters of scarf repaired composite laminates subjected to low-velocity impact with various energy levels at different locations are studied experimentally. The results are compared with those of the original laminates which have no initial damage and don't need repair. The impact load-time history of the specimens, the velocity-time curves of the impactor, the post impact compressive strength of the specimens and the C-scan photographs of the damaged regions are obtained. The delamination threshold load and damage character of the specimen section at impact point are also studied. The results have shown that the impact response of a repaired composite laminate is sensitive to the location of the impact. The impact load and the delamination threshold load have shown different characters for specimens with different impact locations. The debonding characters of the adhesive and compressive strength after impact of the specimens are also influenced by impact locations.

• Steel and Composite Structures
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• v.26 no.6
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• pp.745-757
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• 2018

The inclusion of a ductile steel bracing as means of repairing an earthquake-damaged bridge bent is evaluated and experimentally assessed for the purposes of restoring the damaged bent's strength and stiffness and further improving the energy dissipation capacity. The study is focused on substandard reinforced concrete multi-column bridge bents constructed in the 1950 to mid-1970 in the United States. These types of bents have numerous deficiencies making them susceptible to seismic damage. Large-scale experiments were used on a two-column reinforced concrete bent to impose considerable damage of the bent through increasing amplitude cyclic deformations. The damaged bent was then repaired by installing a ductile fuse steel brace in the form of a buckling-restrained brace in a diagonal configuration between the columns and using post-tensioned rods to strengthen the cap beam. The brace was secured to the bent using steel gusset plate brackets and post-installed adhesive anchors. The repaired bent was then subjected to increasing amplitude cyclic deformations to reassess the bent performance. A subassemblage test of a nominally identical steel brace was also conducted in an effort to quantify and isolate the ductile fuse behavior. The experimental data from these large-scale experiments were analyzed in terms of the hysteretic response, observed damage, internal member loads, as well as the overall stiffness and energy dissipation characteristics. The results of this study demonstrated the effectiveness of utilizing ductile steel bracing for restoring the bent and preventing further damage to the columns and cap beams while also improving the stiffness and energy dissipation characteristics.

• International Journal of Highway Engineering
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• v.15 no.4
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• pp.127-133
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• 2013

PURPOSES : The impact performance of flexible barrier system such as structural response, vehicular motion and occupant safety vary depending on the impact point. Thus, to properly evaluate the performance of a flexible barrier system, impact should be made to a point which will lead to the worst possible results. This point is called the Critical Impact Point (CIP). This paper presents the way to determine the CIP for a SB2 class flexible barrier system which is consisted of Thrie-Beam rail and circular hollow tube post of 2m span. METHODS: Barrier VII simulations were made for impact points; Case 1 at a post, Case 2 at 1/3 span downstream from a post, Case 3 at middle of the span, Case 4 at 2/3 span downstream from a post. For the structural performance (deflections), impact simulation of 8000kg-65km/h-15degree was used, and for vehicle motion and occupant safety, simulation of 1300kg-80km/h-20degree impact was made and analysed. RESULTS: Case 1 gave the largest dynamic deflection of 75.72cm and also gave the largest snag value of 44.3cm. Occupant safety and exit angle of the vehicle after the impact were not sensitive to the impact point and were all below the allowable limit. CONCLUSIONS : For the SB2 class flexible barrier system's CIP can be regarded as a post which is sufficiently away from the end of Length of Need in order to avoid the end-effect of the barrier system. It can be more economic in the long run because the normal concrete pavement material is likely to cost more due to higher probability of maintenance and repair and higher social cost due to traffic accident, etc.

• Molecules and Cells
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• v.40 no.2
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• pp.83-89
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• 2017

Error-free replication and repair of DNA are pivotal to organisms for faithful transmission of their genetic information. Cells orchestrate complex signaling networks that sense and resolve DNA damage. Post-translational protein modifications by ubiquitin and ubiquitin-like proteins, including SUMO and NEDD8, are critically involved in DNA damage response (DDR) and DNA damage tolerance (DDT). The expression of interferon-stimulated gene 15 (ISG15), the first identified ubiquitin-like protein, has recently been shown to be induced under various DNA damage conditions, such as exposure to UV, camptothecin, and doxorubicin. Here we overview the recent findings on the role of ISG15 and its conjugation to target proteins (e.g., p53,$ {\Delta}Np63{\alpha}$, and PCNA) in the control of cellular responses to genotoxic stress, such as the inhibition of cell growth and tumorigenesis.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.11
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• pp.6419-6425
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• 2013

Background: Neoadjuvant chemotherapy (NACT) is a treatment modality whereby chemotherapy is used as the initial treatment of HNSCC in patients presenting with advanced cancer that cannot be treated by other means. It leads to shrinkage of tumours to an operable size without significant compromise to essential oro-facial organs of the patients. The molecular mechanisms behind shrinkage due to NACT is not well elucidated. Materials and Methods: Eleven pairs of primary HNSCCs and adjacent normal epithelium, before and after chemotherapy were screened for cell proliferation and apoptosis. This was followed by immunohistochemical analysis of some cell cycle (LIMD1, RBSP3, CDC25A, CCND1, cMYC, RB, pRB), DNA repair (MLH1, p53) and apoptosis (BAX, BCL2) associated proteins in the same set of samples. Results: Significant decrease in proliferation index and increase in apoptotic index was observed in post-therapy tumors compared to pre-therapy. Increase in the RB/pRB ratio, along with higher expression of RBSP3 and LIMD1 and lower expression of cMYC were observed in post-therapy tumours, while CCND1 and CDC25A remained unchanged. While MLH1 remained unchanged, p53 showed higher expression in post-therapy tumors, indicating inhibition of cell proliferation and induction of apoptosis. Increase in the BAX/BCL2 ratio was observed in post-therapy tumours, indicating up-regulation of apoptosis in response to therapy. Conclusions: Thus, modulation of the G1/S cell cycle regulatory proteins and apoptosis associated proteins might play an important role in tumour shrinkage due to NACT.

• Transactions of the Korean Society of Automotive Engineers
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• v.22 no.4
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• pp.10-19
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• 2014

Despite the movement of safe traffic by the Korean government to reduce deaths in traffic accidents, the casualties increase year by year. In particular, more and more accidents and casualties are reported from car collisions from the back of the vehicles parked for managing traffic accidents on the road, cleaning main roads and medial strips, repairing roads. In order to response to these accidents, the government should take protective measures for road users. In the last decade, seventy-one cases have been reported to occur during highway repair and maintenance. As a result, eight persons were killed and seventy-six were injured, showing the high death rate of 11.3 percent. Therefore, it seems urgent to take some actions against it. The United States and European countries legislate that vehicles of road repair and maintenance should be mandatorily equipped with shock absorber at the back. Korea, however, does not have such legislative measures, which are needed at this time to protect workers on the road. This study compares the performance of the traditional shock absorber for road maintenance vehicles with that of the rear safety guard using air bag, manufactured in accordance with related laws in Korea. Based on the results of the 60km/h rear collision test, this paper proposes improvements in related laws and regulations in an attempt to reduce casualties.

• International conference on construction engineering and project management
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• 2015.10a
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• pp.339-343
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• 2015

To rapidly recover ceased functionality of a facility after a catastrophic seismic event, critical decisions on facility repair works are made within a limited period of time. However, prolonged damage assessment of facilities, due to massive damage in the surrounding region and the complicated damage judgment procedures, may impede restoration planning. To assist reliable structural damage estimation without a deep knowledge and rapid interactive analysis among facility damage and restoration operations during the approximate restoration project planning phase, we developed a prototype of distributed facility restoration simulations through the use of high-level architecture (HLA) (IEEE 1516). The simulation prototype, in which three different simulations (including a seismic data retrieval technique, a structural response simulator, and a restoration simulation module) interact with each other, enables immediate damage estimation by promptly detecting earthquake intensity and the restoration operation analysis according to estimated damage. By conducting case simulations and experiments, research outcomes provide key insights into post-disaster restoration planning, including the extent to which facility damage varies according to disaster severity, facility location, and structures. Additional insights arise regarding the extent to which different facility damage patterns impact a project's performance, especially when facility damage is hard to estimate by observation. In particular, an understanding of required type and amount of repair activities (e.g., demolition works, structural reinforcement, frame installation, or finishing works) is expected to support project managers in approximate work scheduling or resource procurement plans.

• Structural Engineering and Mechanics
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• v.61 no.6
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• pp.701-709
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• 2017

Seismic dissipation devices can play a crucial role in mitigating earthquake damages, loss of life and post-event repair and downtime costs. This research investigates the use of ring springs with high-force-to-volume (HF2V) dissipaters to create damage-free, recentring connections and structures. HF2V devices are passive rate-dependent extrusion-based devices with high energy absorption characteristics. Ring springs are passive energy dissipation devices with high self-centring capability to reduce the residual displacements. Dynamic behaviour of a system with nonlinear structural stiffness and supplemental hybrid damping via HF2V devices and ring spring dampers is used to investigate the design space and potential. HF2V devices are modelled with design forces equal to 5% and 10% of seismic weight and ring springs are modelled with loading stiffness values of 20% and 40% of initial structural stiffness and respective unloading stiffness of 7% and 14% of structural stiffness (equivalent to 35% of their loading stiffness). Using a suite of 20 design level earthquake ground motions, nonlinear response spectra for 8 different configurations are generated. Results show up to 50% reduction in peak displacements and greater than 80% reduction in residual displacements of augmented structure compared to the baseline structure. These gains come at a cost of a significant rise in the base shear values up to 200% mainly as a result of the force contributed by the supplemental devices.

• Steel and Composite Structures
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• v.43 no.3
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• pp.375-388
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• 2022

Nibellen structural system is a novel resilient bracing system based on the application of Bellville disks and Nitinol rods. The cyclic behavior of Nibellen assembly was obtained, and the design equations were developed based on the available literature. Seismic performance of the system was then studied analytically. Two groups of buildings with different lateral force resisting systems were designed and studied: one group with the Nibellen system, and the other with the special concentrically braced frame system. Each building group consisted of 5-, 10-, and 15-story buildings. The Design-Base-Event (DBE) and Maximum Considered Event (MCE) were considered as the seismic hazard, and a suite of seven ground motions were scaled accordingly for response history analyses. Finally, the resiliency of the buildings was studied by obtaining the functionality curve of the buildings before and after the seismic event. The construction cost of the 5-story building with Nibellen bracing system increased but the post-earthquake cost decreased significantly. The application of Nibellen system in the 10- and 15-story buildings reduced both the construction and repair costs, considerably. Resiliency of all the buildings was improved when Nibellen system was used as the lateral force resisting system.