• Molecules and Cells
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• v.26 no.6
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• pp.536-547
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• 2008

Anthocyanidin synthase (ANS, leucoanthocyanidin oxygenase), a 2-oxoglutarate iron-dependent oxygenase, catalyzed the penultimate step in the biosynthesis of the anthocyanin class of flavonoids, from the colorless leucoanthocyanidins to the colored anthocyanidins. The full-length cDNA and genomic DNA sequences of ANS gene (designated as GbANS) were isolated from Ginkgo biloba for the first time. The full-length cDNA of GbANS contained a 1062-bp open reading frame (ORF) encoding a 354-amino-acid protein. The genomic DNA analysis showed that GbANS gene had three exons and two introns. The deduced GbANS protein showed high identities to other plant ANSs. The conserved amino acids (H-X-D) ligating ferrous iron and residues (R-X-S) participating in 2-oxoglutarate binding were found in GbANS at the similar positions like other ANSs. Southern blot analysis indicated that GbANS belonged to a multi-gene family. The expression analysis by real-time PCR showed that GbANS expressed in a tissue-specific manner in G. biloba. GbANS was also found to be up-regulated by all of the six tested abiotic stresses, UV-B, abscisic acid, sucrose, salicylic acid, cold and ethylene, consistent with the promoter region analysis of GbANS. The recombinant protein was successfully expressed in E. coli strain with pET-28a vector. The in vitro enzyme activity assay by HPLC indicated that recombinant GbANS protein could catalyze the formation the cyanidin from leucocyanidin and conversion of dihydroquercetin to quercetin, suggesting GbANS is a bifunctional enzyme within the anthocyanidin and flavonol biosynthetic pathway.

• Molecules and Cells
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• v.39 no.8
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• pp.594-602
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• 2016

Alkyl hydroperoxide reductase subunit C from Pseudomonas aeruginosa PAO1 (PaAhpC) is a member of the 2-Cys peroxiredoxin family. Here, we examined the peroxidase and molecular chaperone functions of PaAhpC using a site-directed mutagenesis approach by substitution of Ser and Thr residues with Cys at positions 78 and 105 located between two catalytic cysteines. Substitution of Ser with Cys at position 78 enhanced the chaperone activity of the mutant (S78C-PaAhpC) by approximately 9-fold compared with that of the wild-type protein (WT-PaAhpC). This increased activity may have been associated with the proportionate increase in the high-molecular-weight (HMW) fraction and enhanced hydrophobicity of S78C-PaAhpC. Homology modeling revealed that mutation of $Ser^{78}$ to $Cys^{78}$ resulted in a more compact decameric structure than that observed in WT-PaAhpC and decreased the atomic distance between the two neighboring sulfur atoms of $Cys^{78}$ in the dimer-dimer interface of S78C-PaAhpC, which could be responsible for the enhanced hydrophobic interaction at the dimer-dimer interface. Furthermore, complementation assays showed that S78C-PaAhpC exhibited greatly improved the heat tolerance, resulting in enhanced1 survival under thermal stress. Thus, addition of Cys at position 78 in PaAhpC modulated the functional shifting of this protein from a peroxidase to a chaperone.

• Research in Plant Disease
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• v.22 no.1
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• pp.25-31
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• 2016

During a field survey in 2014, a Rorippa palustris plant showing virus-like symptom was collected from a pepper field in Dangjin in Korea. The collected sample was subjected to examine infection with pepper-infecting viruses. Molecular diagnosis assay showed that the collected R. palustris sample was co-infected with Pepper mild mottle virus (PMMoV) and Cucumber mosaic virus (CMV). This is the first identification of PMMoV from R. palustris and the first report of CMV infection of R. palustris in Korea. To examine phylogenetic positions of the identified PMMoV and CMV isolates, their complete genome sequences of were determined and compared with those of previously reported isolates of the cognate viruses. Phylogenetic analyses revealed the isolates of PMMoV and CMV obtained from R. palustris are closely related to the pepper isolates of the cognate viruses. Our results suggest that R. palustris could act a weed reservoir of PMMoV and CMV.

• The Plant Pathology Journal
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• v.36 no.4
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• pp.335-345
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• 2020

Tomato grey mould has been one of the destructive fungal diseases during tomato production. Ten mM of menadione sodium bisulfite (MSB) was applied to tomato plants for eco-friendly control of the grey mould. MSB-reduced tomato grey mould in the 3rd true leaves was prolonged at least 7 days prior to the fungal inoculation of two inoculum densities (2 × 10⁴ and 2 × 10⁵ conidia/ml) of Botrytis cinerea. Protection efficacy was significantly higher in the leaves inoculated with the lower disease pressure of conidial suspension compared to the higher one. MSB-pretreatment was not effective to arrest oxalic acid-triggered necrosis on tomato leaves. Plant cell death and hydrogen peroxide accumulation were restricted in necrotic lesions of the B. cinereainoculated leaves by the MSB-pretreatment. Decreased conidia number and germ-tube elongation of B. cinerea were found at 10 h, and mycelial growth was also impeded at 24 h on the MSB-pretreated leaves. MSB-mediated disease suppressions were found in cotyledons and different positions (1st to 5th) of true leaves inoculated with the lower conidial suspension, but only 1st to 3rd true leaves showed decreases in lesion sizes by the higher inoculum density. Increasing MSB-pretreatment times more efficiently decreased the lesion size by the higher disease pressure. MSB led to inducible expressions of defence-related genes SlPR1a, SlPR1b, SlPIN2, SlACO1, SlChi3, and SlChi9 in tomato leaves prior to B. cinerea infection. These results suggest that MSB pretreatment can be a promising alternative to chemical fungicides for environment-friendly management of tomato grey mould.

• MALSORI
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• no.19_20
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• pp.58-87
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• 1990

According to Government Phonology, at 1 phonological positions save the domain's head must be licensed in order to appear in the syllable structure. A non-nuclear head is licensed by the following nucleus, and the nuclei with phonetic content are licensed through government by the nuclear head of the domain at the level of the nuclear projection. Therefore, in the theory of Government Phonology it is claimed that words always end with a nucleus. With regard to the licensing of empty nuclei, Kaye(1990a) proposes the 'Empty Category Principle' and its sub-theory of 'Projection Government'. Government Phonology claims that a nucleus which dominates a vowel that regularly undergoes elision in certain contexts is underlyingly empty. This underlying empty nucleus is not manifested phonetically when it is properly governed by an unlicensed(i, e, a nucleus filled with a full vowel). It is when proper government fails to apply, that the empty nucleus is phonetically Interpreted. The purpose of this paper is to present a principled account of the process of $[i]{\Leftrightarrow}{\emptyset}$ alternation in Korean. Following Kaye's proposal, we assume that [i] of Korean is underlyingly empty. This position is pronounced as [i] if it is unlicensed, and is not phonetically realized if is licensed. Empty nuclei ape devided into two categories: domain-internal and domain-final. Firstly, we consider the question why Korean has little word ending with [i]. As for this, ECP states that domain-final empty nuclei are not pronounced if the language licenses domain-final empty nuclei. Whether a final empty nucleus may occur in the structure is parametric variation. This property is seen from the fact that words may appear to end in consonants in this language. Since Korean abounds with words ending in a consonant, it licenses domain-final empty nuclei. Therefore, it is quite natural that Korean has little word ending with [i]. Secondly, word-internal empty nuclei of Korean respect proper government and inter-onset government. That is, an empty nucleus in word-internal position will be pronounced with the vowel [i] if either proper government or inter-onset government fail to apply. Inter-onset government refers to the government established between two onsets across an empty nucleus. Thirdly, we consider words ending with [i], which seems to be exceptional to the final licensing. Host of them are. either mono-syllabic verbs(for instance, [s'i-] 'to write') or derived adjectives ending with [p'i] (for instance, [kip'i-] 'be happy'). As for the former, the 'inaccessibility for proper government' is applied because the empty nucleus appears in the first syllable. In latter case, domain-final empty nuclei are pronounced as [i] because of government-licensing. That is, final empty nucleus is pronounced to license the preceding onset dominating negatively charmed segments which empty nucleus of Korean cannot license.

• Journal of Mushroom
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• v.10 no.1
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• pp.15-20
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• 2012

Ventilation effects of bottles($1,100m{\ell}$) for culturing Flammulina velutipes on its mycelial growth and mushroom production were investigated. The degree of ventilation were controlled with hole positions, upper and under, and hole sizes in the bottle lids. The ventilation effects were measured with the contents of carbon dioxide, free sugars, chitin, moisture in the bottles and with the amount of produced mushrooms from the bottles. Carbon dioxide concentrations within the culturing bottles at exponential mycelial growth period vertex were relatively high in the bottles with lids without both a sponge and an aeration hole, and in those with a smaller hole. Free sugar contents in the mycelia were the highest in those with a 47mm hole on both sides, and in those with 26~33mm holes only underside. Chitin content was the highest in those with a 26mm hole only underside. On the other hand, the lids with 42mm~47mm holes on the both sides greatly lost water and decreased the mushroom production. In conclusion, the most efficient ventilation hole sizes on the lids for bottle($1,100m{\ell}$) cultivation of Flammulina velutipes using $1,100m{\ell}$ polypropylene bottle were 19mm on both sides of the lid and 26mm on only underside. They produced more mushrooms than the control by 6~9 %.

• Korean Journal of Acupuncture
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• v.23 no.4
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• pp.15-25
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• 2006

Objectives : ${\ulcorner}HuaTaHyunMunNeJoDo{\lrcorner}$ is a medical literature dealing deeply with the theory of internal organs thought to be the core of theories in Chinese medicine. The aim of this study was to explorer on the formation and the organization of ${\ulcorner}HuaTaHyunMunNeJoDo{\lrcorner}$. Methods : We investigated the process of the formation of ${\ulcorner}HuaTaHyunMunNeJoDo{\lrcorner}$ and analyzed the organization of ${\ulcorner}HuaTaHyunMunNeJoDo{\lrcorner}$ on the medical-historical points of view. Results : As a result from studies. Main contents include 11 pieces of pictures about the positions of the regions of acupuncture and moxibustion as well as the internal organs, in addition to descriptions in detail regarding each disease in the internal organs, problems occurred among them, obstinate diseases, etc. Conclusions : A published book in Ming Dynasty and another transcribed by someone in Qing Dynasty as the xylographic books of this literature in China, which turned out to books referred considerably to contents of YangGae's ${\ulcorner}JonJinDo{\lrcorner}$, a famous doctor in Northern Song Dynasty in China. Moreover, the main contents of ${\ulcorner}HuaTaHyunMunNeJoDo{\lrcorner}$ greatly affected the theories of the internal organs for the following generations as they were quoted in Yicheon's ${\ulcorner}UiHakYipMun{\lrcorner}$ in Ming Dynasty in China.

• Fire Science and Engineering
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• v.24 no.5
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• pp.32-38
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• 2010

Multi-dimensional fire dynamics were studied numerically with the change in ventilation conditions in a full-scale ISO 9705 room. Fire Dynamic Simulator (FDS) was used for the identical conditions conducted in previous experiments. Flow rate and doorway width were changed to create over-ventilated fire (OVF) and under-ventilated fire (UVF). From the numerical simulation, it was found that the internal flow pattern rotated in the opposite direction for the UVF relative to the OVF so that a portion of products recirculated to the inside of compartment. Significant change in flow pattern with ventilation conditions may affect changes in the complex process of CO and soot formation inside the compartment due to increase in the residence time of high-temperature products. The fire behavior in the UVF created complex 3D characteristics of species distribution as well as thermal and flow structures. In particular, additional burning near the side wall inside the compartment significantly affected the flow pattern and CO production. The distribution of CO inside the compartment was explained with 3D $O_2$ distribution and flow patterns. It was observed that gas sampling at local positions in the upper layer were insufficient to completely characterize the internal structure of the compartment fire.

• Journal of the Korean Society of Marine Environment & Safety
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• v.22 no.1
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• pp.129-137
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• 2016

In the present study we verified performance of feed-forward control algorithm using short term prediction of ship motion information by taking advantage of developed numerical simulation model of FPSO motion. Up until now, various studies have been conducted about thrust control and allocation for dynamic positioning systems maintaining positions of ships or marine structures in diverse sea environmental conditions. In the existing studies, however, the dynamic positioning systems consist of only feedback control gains using a motion of vessel derived from environmental loads such as current, wind and wave. This study addresses dynamic positioning systems which have feedforward control gain derived from forecasted value of a motion of vessel occurred by current, wind and wave force. In this study, the future motion of vessel is forecasted via Brown's Exponential Smoothing after calculating the vessel motion via a selected mathematical model, and the control force for maintaining the position and heading angle of a vessel is decided by the feedback controller and the feedforward controller using PID theory and forecasted vessel motion respectively. For the allocation of thrusts, the Lagrange Multiplier Method is exploited. By constructing a simulation code for a dynamic positioning system of FPSO, the performance of feedforward control system which has feedback controller and feedforward controller was assessed. According to the result of this study, in case of using feedforward control system, it shows smaller maximum thrust power than using conventional feedback control system.

• Journal of the Korean Society of Marine Environment & Safety
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• v.18 no.5
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• pp.406-415
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• 2012

The research aims to provide its results to each municipal corporation after an in-depth analysis of its optimum demand and also the significance of post-development location selection. The research results, related to the future development of Marina from the municipal corporation, are as follows: There are approximately 49 proper domestic demands after the Marina development. In further detail, there must be a development of the coastal zone Marina concept after classifying the regions to their Metropolitan areas and networking the Marina to each section. Moreover, the classification of the Marinas to small, medium, and large size is of absolute necessity. Regionally, 10 large Marinas must be developed for metropolitan area (2), Chungcheong area (1), Jeolla area (2), Gyeongsang Provinces (3), Gangwon area (1), and Jeju (1). The 17 mid-sized leisure sports Marinas must be developed for metropolitan area (3), Chungcheong area (2), Jeolla area (2), Gyeongsang Provinces (6), Gangwon area (2), and Jeju (2). The rest of 22 small Marinas must be developed for metropolitan area (2), Chungcheong area (1), Jeolla area (6), Gyeongsang Provinces (9), Gangwon area (2), and Jeju (2). In addition, there is an evident result of significant importance in the domestic positions of appropriate Marina development. A group of professionals suggested five key factors contributed to the importance including accessibility, marketability, usability, validity, and natural environment. The results, aimed to show comprehensive importance based on the five factors, ranks in the following order from highest to lowest: usability, accessibility, natural environment, marketability, and validity.