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Isolation and Characterization of Zymomonas mobilis DNA Fragments Showing Promoter Activity in Escherichia coli (Escherichia coli에서 Promoter 활성을 보이는 Zymomonas mobilis DNA 조각의 분리와 분석)

  • Kim, Eun-Joon;Yoon, Ki-Hong;M.Y. Pack
    • Microbiology and Biotechnology Letters
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    • v.17 no.6
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    • pp.600-605
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    • 1989
  • For the purpose of isolation of the Zymomonas mobilis DNA fragments showing promoter activity in Escherichia coli, a promoter screening vector, PCMT215 was constructed by transferring a promoterless chloramphenicol acetyltransferase (CAT) gene of pYEJ001 into pMT21 which contains $\beta$-lactamase gene and multiple cloning sites. A library of Z, mobilis Sau3AI DNA fragments was constructed in E. coli using the newly constructed pCMT215. Fourteen clones showing resistance to chloramphenicol ranging in concentration from 30 to 750 $\mu$g/$m\ell$ were selected. From five clones of them, the Z. mobilis DNA fragments expressing CAT gene of the recombinant plasmids were sequenced and then sites of transcriptional initiation were identified. The nucleotide sequences of the cloned DNA shared AT rich regions, poly A's or T's stretches and palindromic regions. The positions of transcriptional initiation for CAT gene occurred at more than one site spaced over by 4 to 190 base pairs on the cloned fragments in E. coli.

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A Study about TV viewing behavior through Multiple Media under Nscreen circumstances (N스크린 환경에서 다중미디어를 활용한 TV프로그램 이용행태 연구)

  • Kim, Jongha Bell
    • Cartoon and Animation Studies
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    • s.31
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    • pp.177-208
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    • 2013
  • This research analyzes the media which is simultaneously used with TV viewing, the behaviour of multiple media users, and the characteristics of TV programs under Nscreen circumstances. According to the results, TV provides stories and positions it as the main screen (84.5%, n=566), under the Nscreen circumstances. Personal media reproduces stories from TV by utilizing aspects of social media functions such as participation, open communication, community, connectivity, etc. The content is classified under three categories: 1) elicit emotion(point feeling), 2) information production, 3) participation, which is further divided into three subcategories; watch, wish, and result guarantee based on the level of interactivity. This inquiry is based upon diary survey and focus group interviews emphasized the role change of TV, specifically that TV takes a key role in generating original stories with the expansion of screen and social media. Even though users were able to watch the same TV programs at the same time and same place, the different methods of viewing: smartphone, tablet PC, web based mp3 and etc., it reproduced different stories and elicited different characteristics from its TV viewers.

Hand Gesture Recognition Algorithm Robust to Complex Image (복잡한 영상에 강인한 손동작 인식 방법)

  • Park, Sang-Yun;Lee, Eung-Joo
    • Journal of Korea Multimedia Society
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    • v.13 no.7
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    • pp.1000-1015
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    • 2010
  • In this paper, we propose a novel algorithm for hand gesture recognition. The hand detection method is based on human skin color, and we use the boundary energy information to locate the hand region accurately, then the moment method will be employed to locate the hand palm center. Hand gesture recognition can be separated into 2 step: firstly, the hand posture recognition: we employ the parallel NNs to deal with problem of hand posture recognition, pattern of a hand posture can be extracted by utilize the fitting ellipses method, which separates the detected hand region by 12 ellipses and calculates the white pixels rate in ellipse line. the pattern will be input to the NNs with 12 input nodes, the NNs contains 4 output nodes, each output node out a value within 0~1, the posture is then represented by composed of the 4 output codes. Secondly, the hand gesture tracking and recognition: we employed the Kalman filter to predict the position information of gesture to create the position sequence, distance relationship between positions will be used to confirm the gesture. The simulation have been performed on Windows XP to evaluate the efficiency of the algorithm, for recognizing the hand posture, we used 300 training images to train the recognizing machine and used 200 images to test the machine, the correct number is up to 194. And for testing the hand tracking recognition part, we make 1200 times gesture (each gesture 400 times), the total correct number is 1002 times. These results shows that the proposed gesture recognition algorithm can achieve an endurable job for detecting the hand and its' gesture.

Comparison of Load Ratio of Load-cell type Anemometer with Windswept Shape Variation (수풍부 형상에 따른 로드 셀형 풍향풍속계의 하중 비 비교)

  • Kim, Tae-Hyeong;Han, Dong-Seop
    • Journal of Navigation and Port Research
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    • v.36 no.10
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    • pp.839-844
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    • 2012
  • Anemometer is a meteorological instrument that measures wind direction and wind speed in real time, and is mounted to the cranes that are used at ports, shipbuilding yards, off-shore structure, or construction sites that are influenced by wind, and it is used in conjunction with the safety system. Load cell-type anemometer measures the wind direction through the ratio of load between 4 positions by mounting the thin plate to 4 load cells, and measures wind velocity through the summation of loads. According to previous research, the load ratio between two adjacent windswept with respect to the wind direction has unstable value due to vortex around windswept. This causes the result that increases an error on the wind direction. In this study we compared and analyzed the difference between the load ratio with respect to three type windswept shapes in order to suggest the proper windswept shape to reduce this error. The computational fluid flow analysis is carried out with ANSYS CFX to analyze the load ratio between three windswept shapes. Wind direction was adopted as the design variable, and selected 9 wind direction conditions from $0^{\circ}{\sim}90^{\circ}$ with $11.25^{\circ}$ interval for computational fluid flow analysis.

Improved Trajectory Calculation on the Semi-Lagrangian Advection Computation (Semi-Lagrangian 이류항 계산의 추적법 개선)

  • Park, Su-Wan;Baek, Nak-Hoon;Ryu, Kwan-Woo
    • The KIPS Transactions:PartA
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    • v.16A no.6
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    • pp.419-426
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    • 2009
  • To realistically simulate fluid, the Navier-Stokes equations are generally used. Solving these Navier-Stokes equations on the Eulerian framework, the non-linear advection terms invoke heavy computation and thus Semi-Lagrangian methods are used as an approximated way of solving them. In the Semi-Lagrangian methods, the locations of advection sources are traced and the physical values at the traced locations are interpolated. In the case of Stam's method, there are relatively many chances of numerical losses, and thus there have been efforts to correct these numerical errors. In most cases, they have focused on the numerical interpolation processes, even simultaneously using particle-based methods. In this paper, we propose a new approach to reduce the numerical losses, through improving the tracing method during the advection calculations, without any modifications on the Eulerian framework itself. In our method, we trace the grids with the velocities which will let themselves to be moved to the current target position, differently from the previous approaches, where velocities of the current target positions are used. From the intuitive point of view, we adopted the simple physical observation: the physical quantities at a specific position will be moved to the new location due to the current velocity. Our method shows reasonable reduction on the numerical losses during the smoke simulations, finally to achieve real-time processing even with enhanced realities.

A study on monitoring the inner structure of dam body using high resolution seismic reflection method (고분해능 탄성파 반사법을 이용한 댐체 내부구조 모니터링 연구)

  • Kim Jungyul;Kim Hyoungsoo;Oh Seokhoon;Kim Yoosung
    • 한국지구물리탐사학회:학술대회논문집
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    • 2005.05a
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    • pp.15-20
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    • 2005
  • Defects of dam body which can be induced in seepage or leakage procedure can directly affect dam safety. Therefore, a proper inspection method should be carried out in the first place to find out their positions and sizes, After that, some reinforcement works such as grouting and the corresponding assessment could be taken in a proper way. The dam(center core type earth dam) issued in this study has been in need for intensive diagnosis and reinforcement work, because a lot of slumps similar to cracks, seepage and some boggy area have been observed on the downstream slope. High resolution seismic reflection method was performed on the crest profile twice before and after grouting work(Aug. 2001 and Nov. 2004) aimed at the dam inspection and the assessment of grouting efficiency as well. To enhance the data resolution, P-beam energy radiation technique which can reduce the surface waves and hence to reinforce the reflection events was used. Strong reflection events were recognized in the stack section before grouting work, It seems that the events would be caused by e.g. horizontal cracks with a considerable aperture, Meanwhile such strong reflection events were not observed in the section after grouting. That is, the grouting work was dear able to reinforce the defects of dam body. Hence, the section showed an well arranged picture of dam inner structure. In this sense, seismic reflection method will be a desirable technique for dam inspection and for monitoring dam inner structure as well.

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A Study on the Compensation of the Difference of Driving Behavior between the Driving Vehicle and Driving Simulator (가상주행과 실차주행의 운전자 주행행태 차이에 관한 연구)

  • Park, Jinho;Lim, Joonbeom;Joo, Sungkab;Lee, Soobeom
    • International Journal of Highway Engineering
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    • v.17 no.2
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    • pp.107-122
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    • 2015
  • PURPOSES : The use of virtual driving tests to determine actual road driving behavior is increasing. However, the results indicate a gap between real and virtual driving under same road conditions road based on ergonomic factors, such as anxiety and speed. In the future, the use of virtual driving tests is expected to increase. For this reason, the purpose of this study is to analyze the gap between real and virtual driving on same road conditions and to use a calibration formula to allow for higher reliability of virtual driving tests. METHODS : An intelligent driving recorder was used to capture real driving. A driving simulator was used to record virtual driving. Additionally, a virtual driving map was made with the UC-Win/Road software. We gathered data including geometric structure information, driving information, driver information, and road operation information for real driving and virtual driving on the same road conditions. In this study we investigated a range of gaps, driving speeds, and lateral positions, and introduced a calibration formula to the virtual record to achieve the same record as the real driving situation by applying the effects of the main causes of discrepancy between the two (driving speed and lateral position) using a linear regression model. RESULTS: In the virtual driving test, driving speed and lateral position were determined to be higher and bigger than in the real Driving test, respectively. Additionally, the virtual driving test reduces the concentration, anxiety, and reality when compared to the real driving test. The formula includes four variables to produce the calibration: tangent driving speed, curve driving speed, tangent lateral position, and curve lateral position. However, the tangent lateral position was excluded because it was not statistically significant. CONCLUSIONS: The results of analyzing the formula from MPB (mean prediction bias), MAD (mean absolute deviation) is after applying the formula to the virtual driving test, similar to the real driving test so that the formula works. Because this study was conducted on a national, two-way road, the road speed limit was 80 km/h, and the lane width was 3.0-3.5 m. It works in the same condition road restrictively.

DEVELOPMENT OF AN AMPHIBIOUS ROBOT FOR VISUAL INSPECTION OF APR1400 NPP IRWST STRAINER ASSEMBLY

  • Jang, You Hyun;Kim, Jong Seog
    • Nuclear Engineering and Technology
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    • v.46 no.3
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    • pp.439-446
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    • 2014
  • An amphibious inspection robot system (hereafter AIROS) is being developed to visually inspect the in-containment refueling storage water tank (hereafter IRWST) strainer in APR1400 instead of a human diver. Four IRWST strainers are located in the IRWST, which is filled with boric acid water. Each strainer has 108 sub-assembly strainer fin modules that should be inspected with the VT-3 method according to Reg. guide 1.82 and the operation manual. AIROS has 6 thrusters for submarine voyage and 4 legs for walking on the top of the strainer. An inverse kinematic algorithm was implemented in the robot controller for exact walking on the top of the IRWST strainer. The IRWST strainer has several top cross braces that are extruded on the top of the strainer, which can be obstacles of walking on the strainer, to maintain the frame of the strainer. Therefore, a robot leg should arrive at the position beside the top cross brace. For this reason, we used an image processing technique to find the top cross brace in the sole camera image. The sole camera image is processed to find the existence of the top cross brace using the cross edge detection algorithm in real time. A 5-DOF robot arm that has multiple camera modules for simultaneous inspection of both sides can penetrate narrow gaps. For intuitive presentation of inspection results and for management of inspection data, inspection images are stored in the control PC with camera angles and positions to synthesize and merge the images. The synthesized images are then mapped in a 3D CAD model of the IRWST strainer with the location information. An IRWST strainer mock-up was fabricated to teach the robot arm scanning and gaiting. It is important to arrive at the designated position for inserting the robot arm into all of the gaps. Exact position control without anchor under the water is not easy. Therefore, we designed the multi leg robot for the role of anchoring and positioning. Quadruped robot design of installing sole cameras was a new approach for the exact and stable position control on the IRWST strainer, unlike a traditional robot for underwater facility inspection. The developed robot will be practically used to enhance the efficiency and reliability of the inspection of nuclear power plant components.

Four Year Experience with Valve Replacement of Valvular Heart Diseases (심장판막 치환술후 단기 추적성적)

  • 류한영
    • Journal of Chest Surgery
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    • v.23 no.6
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    • pp.1180-1190
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    • 1990
  • 96 patients underwent cardiac valve replacement for valvular heart diseases consecutively between February 1986 to February 1990 in the Department of Thoracic and Cardiovascular Surgery of Yeungnam University Hospital. The follow up period was between 6 months and 4.5 years postoperatively[mean 23.4$\pm$13.1 months]. 75 cases got mitral valve replacement, 6 cases, aortic valve replacement, 15 cases, double valve replacement. 30[31.2%] patients were male and 66[68.8%] were female and the age ranged from 14 to 66 years old. Early hospital death within 30 days postoperation were 5 patients[5.2%], consisting of by low cardiac output in 2, infective endocarditis in 1, multiple organ failure with sepsis in 1 patient. There was no late postoperative death. Most common early postoperative complication was wound disruption [8.7%] and then low cardiac output, pneumothorax, pleural effusion in order. Most common late postoperative complications were minor bleeding episodes[8.7%] related to anticoagulant therapy which were consisted of frequent epistaxis in 3, gum bleeding in 2, hemorrhagic gastritis in 1, hypermenorrhea in 1, hematoma in right arm in 1 patient. Valve-related complications included valve thrombosis [1.6%/ patient-year], valve failure due to pannus formation[1.1% /patient-year], prosthetic valve endocarditis[1, 1%o/patient-year] and minor anticoagulant hemorrhage[4.4% /patient-year]. 5 cases of reoperations were performed in 4 patients due to valve failure and all of them were in the mitral positions[2.7% /patient-year]. Cardiothoracic ratios in the chest X-ray decreased at the 6th month and 1st year postoperation in all patients. But in New York Heart Association[NYHA] functional class IV, no change in cardiothoracic ratio was found between 6 months and 1 year postoperation. In the echocardiogram, the size of the cardiac chambers decreased, but ejection fraction increased postoperatively in each functional class. In the electrocardiogram, decreases were found in the incidence of atrial fibrillation, left atrial enlargement, left ventricular hypertrophy with right bundle branch block increasing postoperatively in each functional class. The actuarial survival rate was 98.4% for all patients, 98.7% for mitral valve replacement, 83.8% for aortic valve replacement, and 80% for double valve replacement at the end of a 4.5 year follow up period. Meanwhile the actuarial freedom rate was 91.5% for prosthetic valve endocarditis, 91.6% for thromboembolism, 89.0% for prosthetic valve failure and 83.7% for minor anticoagulant hemorrhage. Preoperative NYHA class III and IV were 75% of all patients, but 95% of all patients were up graded to NYHA class I and II postoperatively.

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A Study on the development of leadership training program for first-line nurse managers (일선 간호관리자를 위한 리더십 훈련 프로그램 개발)

  • Koh, Myung-Suk;Han, Sung-Suk
    • Journal of Korean Academy of Nursing Administration
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    • v.6 no.3
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    • pp.333-345
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    • 2000
  • The health care environment becomes more competitive every day. It has fallen to nurse managers - from vice presidents of patient care to nurse managers and their assistants - to recruit and develop a workforce that successfully meets the needs of both patients and the organization. This means employees who demonstrate advanced critical thinking skills, creative problem solving, and sound decision making skills combined with clinical skills and patient advocacy. The environment which nurse managers create and the way they relate to their workforce, are pivotal to organizational viability. Especially leadership of first -line nurse managers contributes to the success of their organizations. First-line nurse managers are deserved to be one of the most administrative supervisors through the middle stratum in a hospital organization as being a manager in the field service if assessed from the overall aspects of hospital, as being an interim managers in the nursing department as well as being a supreme supervisor in a unit in terms of an organizational structure in the hospital. Similarly, as a compete leader, the first-line nurse managers have not only a professional which is qualified to perform a role of appropriate coordination with medical staff and key personnel but also hold an important key position a being responsible for performing his or her given role. The first-line nurse manager is expected to manage human and fiscal resources in ways not required before. While an identified need for well-prepared first-line nurse manager continues to plague the profession, first-line nurse managers often have difficulty providing the leadership required. The need leadership training to function effectively in their positions. But we hardly find a useful leadership training program for first-line nurse managers, therefore the purpose of this study was to developed the leadership training program for them. The steps of leadership program development were below: 1st step, 2 studies were done before develop a leadership program. One was done to ask to first-line nurse managers what they want to learn through leadership training, the other one was to ask the staff nurses what their opinions are for their first-line nurse managers leadership. 2nd step was searching other leadership programs contents. The results of this study were below: The total amount of hours is 24. Leadership training program contents are : Future of nursing profession (210min), understanding basic factor's of leadership and leadership theories(310 min), self understanding as first- line nurse managers(320 min), basic principle and practice of interpersonal relationship(210 min), assertiveness training, conflict management (180min), and group study(210min). This is challenging time to be a leader, especially in nursing. As nurse managers look toward the new millennium, it seems as through the same struggles are ahead that are behind. So nurse managers need to embrace change with a positive attitude. They need to demonstrate risk taking and support it in their staffs. All these things are possible that after they participate the leadership training program.

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