• FOCUS: LIFE SCIENCE
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• no.1
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• pp.3.1-3.7
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• 2024

The article discusses the critical role of chromatography in the analysis and purification of proteins in biopharmaceuticals, emphasizing the importance of comprehensive characterization for ensuring their safety and efficacy. It highlights the use of Avantor® ACE® HPLC columns for the separation and purification of proteins, focusing on the analysis of intact proteins using reversed-phase liquid chromatography (RPLC) with fully porous particles. This article also details the application of different mobile phase additives, such as TFA and formic acid, and emphasizes the advantages of using type B ultra-pure silica-based columns for efficiency and peak shape in biomolecule analysis. Additionally, it addresses the challenges of analyzing intact proteins due to slow molecular diffusion and introduces the concept of solid-core (or superficially porous) particles, emphasizing their benefits over traditional porous particles for the analysis of therapeutic proteins. Furthermore, it discusses the development of Avantor® ACE® UltraCore BIO columns, specifically designed for the high-efficiency separation of large biomolecules, such as proteins, and demonstrates their effectiveness in achieving high-resolution separations, even for higher molecular weight proteins like monoclonal antibodies (mAbs). In addition, it underscores the complexity of analyzing and characterizing intact protein biopharmaceuticals, requiring a range of analytical techniques and the use of wide-pore stationary phases, operated at elevated temperatures and with relatively shallow gradients. It highlights the comprehensive range of options offered by Avantor® ACE® wide pore columns, including both fully porous and solid-core particles, bonded with a variety of complementary stationary phase chemistries to optimize selectivity during method development. The use of ultrapure and highly inert base silica is emphasized for enabling the use of lower concentrations of mobile phase modifiers without compromising analyte peak shape, particularly beneficial for LC-MS applications. Then the article concludes by emphasizing the significance of reversed-phase liquid chromatography and its compatibility with mass spectrometry as a valuable tool for the separation and analysis of intact proteins and their closely related variants in biopharmaceuticals.

• Journal of The Korean Society of Agricultural Engineers
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• v.59 no.4
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• pp.43-52
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• 2017

This study analyzed pore water pressure, earth pressure and settlement through laboratory model tests in order to suggest the effective remodeling method in the case of reinforcing the upstream and downstream slope of deteriorated reservoirs that has no cores and filters or is not functional. The method of remodeling the upstream slope using dredge soil is first prevent seepage by installing the core, and the leakage water can be rapidly discharged through a filter installed on the downstream slope. Therefore, it is considered a highly efficient method of remodeling that reduces piping phenomena and increasing the storage capacity of the reservoir. The variation of earth pressure without the core and filter was greater than with it, while the change largely showed in the upstream slope, the downstream slope did not show any significant changes. The remodeling method of the downstream slope with the core appeared differently pore water pressure depending on the presence of the vertical and horizontal filters. In the upstream slope, the pore water pressure rises sharply, the base and middle gradually increased, and the downstream slope appeared small. The pore water pressure of embankment with a vertical and horizontal filter will be smaller than without it. The remodeling of deteriorated reservoir that does not have the function of the filter, the vertical filter must be installed in a position that is higher than the expected seepage line by removing portions of the downstream slopes. Since the horizontal filter is an important structure that provides stable drainage during an earthquake or concentrated leak, it is necessary to examine any change in the seepage characteristics depending on the filter intervals via three-dimensional finite element analysis, and it should be connected to the tow-drain to reduce the possibility of the collapse of the reservoir.

• Journal of Powder Materials
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• v.27 no.1
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• pp.37-43
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• 2020

The Fe-22wt.%Cr-6wt.%Al foams were fabricated via the powder alloying process in this study. The structural characteristics, microstructure, and mechanical properties of Fe-Cr-Al foams with different average pore sizes were investigated. Result of the structural analysis shows that the average pore sizes were measured as 474 ㎛ (450 foam) and 1220 ㎛ (1200 foam). Regardless of the pore size, Fe-Cr-Al foams had a Weaire-Phelan bubble structure, and α-ferrite was the major constituent phase. Tensile and compressive tests were conducted with an initial strain rate of 10^-3/s. Tensile yield strengths were 3.4 MPa (450 foam) and 1.4 MPa (1200 foam). Note that the total elongation of 1200 foam was higher than that of 450 foam. Furthermore, their compressive yield strengths were 2.5 MPa (450 foam) and 1.1 MPa (1200 foam), respectively. Different compressive deformation behaviors according to the pore sizes of the Fe-Cr-Al foams were characterized: strain hardening for the 450 foam and constant flow stress after a slight stress drop for the 1200 foam. The effect of structural characteristics on the mechanical properties was also discussed.

• Journal of the Korean Chemical Society
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• v.16 no.6
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• pp.341-348
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• 1972

The significance of the curvature dependency correction of surface tension is studied in calculating the pore volume distribution of porous adsorbent from nitrogen adsorption isotherm. That is, Kelvin radii are calculated with curvature dependent surface tension values calculated by Chang et al, and then with these Kelvin radii, pore volume distributions of three porous adsorbents, silica alumina (steam deactivated), silica gel (Davidson 59), and silica gel (Mallinc-krodt Standard Luminescent), are calculated. The results are compared with those obtained by the previous method in which surface tension is taken as constant and also with the others. obtained by the modelless method proposed by Brunauer et al. The maximum point of the distribution curve shift to the larger pore radius, when the curvature dependency is considered. Furthermore, the relative pressure at which capillary condensation commences is by far the lower than that accepted previously. This effect becomes significant as the pore radius approaches to the micropore range.

• Restorative Dentistry and Endodontics
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• v.46 no.4
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• pp.57.1-57.9
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• 2021

Objectives: This study aimed to compare and evaluate the porosity and pore size distribution of high-viscosity glass ionomer cements (HVGICs) and conventional glass ionomer cements (GICs) using micro-computed tomography (micro-CT). Materials and Methods: Forty cylindrical specimens (n = 10) were produced in standardized molds using HVGICs and conventional GICs (Ketac Molar Easymix, Vitro Molar, MaxxionR, and Riva Self-Cure). The specimens were prepared according to ISO 9917-1 standards, scanned in a high-energy micro-CT device, and reconstructed using specific parameters. After reconstruction, segmentation procedures, and image analysis, total porosity and pore size distribution were obtained for specimens in each group. After checking the normality of the data distribution, the Kruskal-Wallis test followed by the Student-Newman-Keuls test was used to detect differences in porosity among the experimental groups with a 5% significance level. Results: Ketac Molar Easymix showed statistically significantly lower total porosity (0.15%) than MaxxionR (0.62%), Riva (0.42%), and Vitro Molar (0.57%). The pore size in all experimental cements was within the small-size range (< 0.01 mm³), but Vitro Molar showed statistically significantly more pores/defects with a larger size (> 0.01 mm³). Conclusions: Major differences in porosity and pore size were identified among the evaluated GICs. Among these, the Ketac Molar Easymix HVGIC showed the lowest porosity and void size.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.26 no.5A
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• pp.927-934
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• 2006

According to the high demand of concrete structures with high performance, various studies have examined on the high performance concrete, especially high strength concrete. Various admixtures are required to produce high strength concrete and silica fume has been the most popular admixture. Recently, however, metakaolin, which is similar to silica fume in properties but cheaper, has been introduced to high strength concrete. This study conducted XRD and SEM analyses on a cement paste specimens to clarify metakaolin's performance in pozzolan. Additionally, a concrete specimens were fabricated to analyze its pore structure using Mercury Intrusion Porosimetry and its correlation to the compressive strength. In result, it was found that the average diameter of pore reduced and compressive strength increased as more metakaolin content was added. In addition, a regression analysis of $10nm{\sim}10{\mu}m$ pore and compression strength revealed that these two factors had a high correlation of about 0.93 and 10~15% of metakaolin replacement was most appropriate.

• Journal of the Korean Geosynthetics Society
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• v.21 no.1
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• pp.1-10
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• 2022

In this study, a model parameter evaluation method using relative density was proposed to utilize applicable UBC3D-PLM for liquefaction behavior. In addition, dynamic effective stress analysis, that is, liquefaction analysis, was performed on the case of the liquefaction occurrence region where acceleration and pore water pressure were measured, and compared with the actual measurement and the existing Finn analysis results. Through this study, it was found that the proposed method can easily evaluate the necessary parameters required by the related model and predict the pore water pressure behavior in the region where liquefaction occurs. In addition, in the case of the study area, both measurements and numerical analysis showed that liquefaction occurred when a certain amount of time elapsed after the earthquake acceleration reached the maximum value. In the case of UBC3D-PLM applied in this study, the excess pore water pressure behavior similar to the actual measurement was predicted, and the occurrence of liquefaction was evaluated in the same way as the actual measurement. In particular, although the excess pore water pressure in the sand layer was greater, the phenomenon in which liquefaction occurred in the silt layer was accurately realized. It is expected that the proposed model parameter evaluation method and finite element analysis applying UBC3D-PLM can be used to select the liquefaction reinforcement region in the future seismic design and reinforcement by evaluating the liquefaction occurrence region similarly to the real one.

• Journal of the Korean Wood Science and Technology
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• v.36 no.4
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• pp.44-51
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• 2008

In this study, the effects of carbonization temperature on the physico-chemical properties of Korean red-pine wood (Pinus densiflora S. et Z.) powder charcoal are studied by elemental analysis, nitrogen adsorption-desorption and SEM techniques. The surface structure and physico-chemical properties of the wood charcoal greatly depend on the carbonization temperature and their temperature dependences for sapwood (swd) and heartwood (hwd) are qualitatively analogous. Because of the differences in characteristics such as hardness and composition between heartwood and sapwood, charcoals from heartwood have larger specific surface area and smaller average pore diameter than that from sapwood. Because the decomposition reaction mostly proceeds in the precarbonization stage, the charcoal produced in this stage mainly consists of carbon. The second carbonization reaction is insignificant but still proceeds up to $700^{\circ}C$, and the specific surface area continuously increases. Above $800^{\circ}C$, the surface area is reduced by the pore-filling and narrowing effects and especially above $900^{\circ}C$, new carbon phase with hexagonal column rooted into the pore is formed. The nitrogen adsorption-desorption isotherm of the charcoal is classified as type I and its hysteresis loop was as type H4.

• Journal of Korean Tunnelling and Underground Space Association
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• v.5 no.2
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• pp.175-187
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• 2003

This paper presents the effect of groundwater on tunnel performance. Fundamental issues in tunneling under high groundwater table together with an illustrative example are discussed. The effect of groundwater on tunnel excavation was examined using a 3D stress-pore pressure coupled finite-element analysis. The results of the 3D coupled analysis were then compared with those of a total stress analysis. Examined items included earth and pore pressures around lining, and lining stresses. Also the examined include face movement and ground surface movements. The results indicated that the effect of groundwater on tunnel excavation can only be captured through a fully coupled analysis. Implementations of the findings from this study are discussed in great detail.

• Journal of the Korean Geotechnical Society
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• v.30 no.1
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• pp.103-116
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• 2014

This paper presents the results of a numerical investigation on the load carrying capacity and consolidation behavior of suction piles. Three dimensional numerical models which reflect realistic ground conditions and installation procedures including the ground-suction pile interface were adopted to conduct a parametric study on variables such as the length-diameter ratio and the loading configurations, i.e, vertical, horizontal, and combined loads. The results indicated that the load carrying capacity of a suction pile can only be realistically obtained when the interface behavior between the suction pile and the ground is correctly modeled. Also carried out was the stress-pore pressure coupled analysis to investigate the consolidation behavior of the suction pile after the application of a vertical loading. Based on the results, failure envelops and associated equations were developed, which can be used to estimate load carrying capacity of suction piles installed in similar conditions considered in this study. The results of consolidation analysis based on the stress-pore pressure coupled analysis indicate that no significant excess pore pressure and associated consolidation settlement occur for the loading configuration considered in part due to the load transfer mechanism of the suction pile.