• Title/Summary/Keyword: population variability

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Population Genetics of Plasmodium vivax in Four High Malaria Endemic Areas in Thailand

  • Congpuong, Kanungnit;Ubalee, Ratawan
    • Parasites, Hosts and Diseases
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    • v.55 no.5
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    • pp.465-472
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    • 2017
  • Recent trends of malaria in Thailand illustrate an increasing proportion of Plasmodium vivax, indicating the importance of P. vivax as a major causative agent of malaria. P. vivax malaria is usually considered a benign disease so the knowledge of this parasite has been limited, especially the genetic diversity and genetic structure of isolates from different endemic areas. The aim of this study was to examine the population genetics and structure of P. vivax isolates from 4 provinces with different malaria endemic settings in Thailand using 6 microsatellite markers. Total 234 blood samples from P. vivax mono-infected patients were collected. Strong genetic diversity was observed across all study sites; the expected heterozygosity values ranged from 0.5871 to 0.9033. Genetic variability in this study divided P. vivax population into 3 clusters; first was P. vivax isolates from Mae Hong Son and Kanchanaburi Provinces located on the western part of Thailand; second, Yala isolates from the south; and third, Chanthaburi isolates from the east. P. vivax isolates from patients having parasite clearance time (PCT) longer than 24 hr after the first dose of chloroquine treatment had higher diversity when compared with those having PCT within 24 hr. This study revealed a clear evidence of different population structure of P. vivax from different malaria endemic areas of Thailand. The findings provide beneficial information to malaria control programme as it is a useful tool to track the source of infections and current malaria control efforts.

Population Structure and Genetic Bottleneck Analysis of Ankleshwar Poultry Breed by Microsatellite Markers

  • Pandey, A.K.;Kumar, Dinesh;Sharma, Rekha;Sharma, Uma;Vijh, R.K.;Ahlawat, S.P.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.7
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    • pp.915-921
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    • 2005
  • Genetic variation at 25 microsatellite loci, population structure, and genetic bottleneck hypothesis were examined for Ankleshwar poultry population found in Gujrat, India. The estimates of genetic variability such as effective number of alleles and gene diversities revealed substantial genetic variation frequently displayed by microsatellite markers. The average polymorphism across the studied loci and the expected gene diversity in the population were 6.44 and 0.670${\pm}$0.144, respectively. The population was observed to be significantly differentiated into different groups, and showed fairly high level of inbreeding (f = 0.240${\pm}$0.052) and global heterozygote deficit. The bottleneck analysis indicated the absence of genetic bottleneck in the past. The study revealed that the Ankleshwar poultry breed needs appropriate genetic management for its conservation and improvement. The information generated in this study may further be utilized for studying differentiation and relationships among different Indian poultry breeds.

Characteristics of Morphological and Production from Different Origin of Foeniculum vulgare Mill.

  • Chung, Hae-Gon;Kim, Seong-Min;Nemeth, E.
    • Korean Journal of Medicinal Crop Science
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    • v.11 no.4
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    • pp.259-263
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    • 2003
  • This study was carried out to get basic information about the morphological and production characteristics of fennel populations different origin under different ecological conditions in Hungary. The Korean population can be registered as a medium high genotype and showed a more accelerated growth characters than Hungarian taxon and the two populations have a medium homogeneity $(CV\;=\;4{\sim}22%)$. In the second year plant height in Hungarian taxon was significantly higher than that of the Korean population. The shooting dynamic of individual plants might be heterogeneous in both taxa and the Korean population was not only shorter in the height but had also less shoots by 16% than the Hungarian one. The Hungarian taxon exceeded the umbel size and numbers to the Korean one (by 3.62 cm in the average) and the variability in the populations was high $(CV\;=\;29{\sim}49%)$. Seed size was proved to be also a discriminative feature between the examined taxa. With small deviations (CV < 2) the Hungarian population produced seeds longer by 38%. Homogeneity in the populations was dependent more on the vegetation years and on the characteristics measured but less on the origins, respectively.

Estimation of the Number of Sampling Points Required for the Determination of Soil CO2 Efflux in Two Types of Plantation in a Temperate Region

  • Lee, Na-Yeon(Mi-Sun);Koizumi, Hiroshi
    • Journal of Ecology and Environment
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    • v.32 no.2
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    • pp.67-73
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    • 2009
  • Soil $CO_2$ efflux can vary markedly in magnitude over both time and space, and understanding this variation is crucial for the correct measurement of $CO_2$ efflux in ecological studies. Although considerable research has quantified temporal variability in this flux, comparatively little effort has focused on its spatial variability. To account for spatial heterogeneity, we must be able to determine the number of sampling points required to adequately estimate soil $CO_2$ efflux in a target ecosystem. In this paper, we report the results of a study of the number of sampling points required for estimating soil $CO_2$ efflux using a closed-dynamic chamber in young and old Japanese cedar plantations in central Japan. The spatial heterogeneity in soil $CO_2$ efflux was significantly higher in the mature plantation than in the young stand. In the young plantation, 95% of samples of 9 randomly-chosen flux measurements from a population of 16 measurements made using 72-$cm^2$ chambers produced flux estimates within 20% of the full-population mean. In the mature plantation, 20 sampling points are required to achieve means within $\pm$ 20% of the full-population mean (15 measurements) for 95% of the sample dates. Variation in soil temperature and moisture could not explain the observed spatial variation in soil $CO_2$ efflux, even though both parameters are a good predictor of temporal variation in $CO_2$ efflux. Our results and those of previous studies suggest that, on average, approximately 46 sampling points are required to estimate the mean and variance of soil $CO_2$ flux in temperate and boreal forests to a precision of $\pm$ 10% at the 95% confidence level, and 12 points are required to achieve a precision of $\pm$ 20%.

Pre-service Teachers' Understanding of Statistical Sampling (예비교사들의 통계적 표집에 대한 이해)

  • Ko, Eun-Sung;Lee, Kyeong-Hwa
    • Journal of Educational Research in Mathematics
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    • v.21 no.1
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    • pp.17-32
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    • 2011
  • This study investigated pre-service teachers' understanding of statistical sampling. The researchers categorized major topics related to sampling into representativeness of samples, sampling variability, and sampling distribution, and selected concepts connected to each topic. Findings on this study are as follows: Even though most of the pre-service teachers considered the random sampling bringing unbiased outcomes as a proper sampling method, only 64% of them recognized that sample is a quasi-proportional, small-scale version of population; Few pre-service teachers understood that more important is the size of sample, not the portion of sample to population, and half of them appreciated that the number of sampling has a powerful effect on drawing of reliable results than the size of sample; Few pre-service teachers understood that sampling distribute is irrelevant to the shape of population and has a symmetrical bell-shape.

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Determination of Genetic Divergence Based on DNA Markers Amongst Monosporidial Strains Derived from Fungal Isolates of Karnal Bunt of Wheat

  • Seneviratne, J.M.;Gupta, Atul K.;Pandey, Dinesh;Sharma, Indu;Kumar, Anil
    • The Plant Pathology Journal
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    • v.25 no.4
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    • pp.303-316
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    • 2009
  • Genetic variation among the base isolates and monosporidial strains derived from these isolates of Tilletia indica- the causal agent of Karnal bunt (KB) in wheat, was analyzed by morphological, growth behaviors and RAPD-ISSR based molecular polymorphism. Genetic make up of fungal cultures vary among each other. The magnitude of variation in KBPN group is less (narrow genetic base) when compared to the other groups KB3, KB9 and JK (broad genetic base) reflecting that variability is a genetically governed process. The generation of new variation with different growth characteristics is not a generalized feature and is totally dependant on the original genetic make-up of the base isolate generating new monosporidial strains. Thus, it can be concluded that monosporidial strains derived from mono-teliosporic isolate, consists of genetically heterogeneous population. The morphological and genetic variability further suggests that the variation in T. indica strains is predominantly derived through the genetic rearrangements through para sexual means.

Morphological Studies on the Jack Mackerel Populations

  • Kim, Wan Soo
    • 한국해양학회지
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    • v.4 no.1
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    • pp.17-35
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    • 1969
  • This work concerns morphological studies of the Japaness jack mackerel, Trachurus japonicus(Temminck & Schlegel). A total of 48 random samples consisting of 2669 fish is examined, representing three geographic regions, namely, Kamakura, Nagasaki, and Tottori. Preservation affects length and weight of fish considerably, but the variability after 10 days is shown to be negligible. The variability in measuring the morphometric characters due to different measures and orders is found to be significant. They are relatively small, however, compared to the variability in mean length to be expected in samples drawn from the population. Two meristic characters, namely, anal and second dorsal fin rays and three morphometric characters, namely, head length, first and second dorsal insertion distances are chosen for the study. Results of the statistical analysis reveal that differences in the selected meristic characters among samples within and between regions are found to be significant. In general, it is reasonable to state that the jack mackerel collected in Nagasaki region, on the average, have greatest number of anal fin ray and second dorsal fin ray followed by Kamakura, and Tottori regions in that order. It is found that although no significant differences in the slope of regression line are noticed, the mean differences of the selected morphometric measurements in relation to fish length are found to be significant among samples within each region. Differences in the regression coefficients as well as the adjusted sample means are found to be significant between regions. The analysis suggests that samples from Nagasaki region, on the average, have longest head length and greatest distances from snout to the insertion of first dorsal and second dorsal fins followed by Kamakura, and Tottori regions in that order.

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Statistical evaluation of drift demands of rc frames using code-compatible real ground motion record sets

  • Kayhan, Ali Haydar;Demira, Ahmet
    • Structural Engineering and Mechanics
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    • v.60 no.6
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    • pp.953-977
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    • 2016
  • Modern performance-based design methods require ways to determine the factual behavior of structures subjected to earthquakes. Drift ratio demands are important measures of structural and/or nonstructural damage of the structures in performance-based design. In this study, global drift ratio and interstory drift ratio demands, obtained by nonlinear time history analysis of three generic RC frames using code-compatible ground motion record sets, are statistically evaluated. Several ground motion record sets compatible with elastic design spectra defined for the local soil classes in Turkish Earthquake Code are used for the analyses. Variation of the drift ratio demands obtained from ground motion records in the sets and difference between the mean of drift ratio demands calculated for ground motion sets are evaluated. The results of the study indicate that i) variation of maximum drift ratio demands in the sets were high; ii) different drift ratio demands are calculated using different ground motion record sets although they are compatible with the same design spectra; iii) the effect of variability due to random causes on the total variability of drift ratio demands is much larger than the effect of variability due to differences between the mean of ground motion record sets; iv) global and interstory drift ratio demands obtained for different ground motion record sets can be accepted as simply random samples of the same population at %95 confidence level. The results are valid for all the generic frames and local soil classes considered in this study.

Effect of Grid Cell Size on the Accuracy of Dasymetric Population Estimation (격자크기가 밀도구분적 인구추정의 정확성에 미치는 영향)

  • JUN, Byong-Woon
    • Journal of the Korean Association of Geographic Information Studies
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    • v.19 no.3
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    • pp.127-143
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    • 2016
  • This study explored the variability in the accuracy of dasymetric population estimation with different grid cell sizes. Dasymetric population maps for Fulton County, Georgia in the US were generated from 30m to 420m at intervals of 30m using an automated intelligent dasymetric mapping technique, population data, and original and simulated land use and cover data. The accuracies of dasymetric population maps were evaluated using RMSE and adjusted RMSE statistics. Lumped fractal dimension values were calculated for the dasymetric population maps generated from resolutions of 30m to 420m using the triangular prism surface area (TPSA) method. The results show that a grid cell size of 210m or smaller is required to estimate population more accurately in terms of thematic accuracy, but a grid cell size of 30m is required to meet an acceptable spatial accuracy of dasymetric population estimation in the study area. The fractal analysis also indicates that a grid cell size of 120m is the optimal resolution for dasymetric population estimation in the study area.

Extent of linkage disequilibrium and effective population size of Korean Yorkshire swine

  • Shin, Donghyun;Won, Kyeong-Hye;Kim, Sung-Hoon;Kim, Yong-Min
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.12
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    • pp.1843-1851
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    • 2018
  • Objective: We aimed to characterize linkage disequilibrium (LD) and effective population size ($N_e$) in a Korean Yorkshire population using genomic data from thousands of individuals. Methods: We genotyped 2,470 Yorkshire individuals from four major Grand-Grand-Parent farms in Korea using the Illumina PorcineSNP60 version2 BeadChip, which covers >61,565 single nucleotide polymorphisms (SNPs) located across all chromosomes and mitochondria. We estimated the expected LD and inferred current $N_e$ as well as ancestral $N_e$. Results: We identified 61,565 SNP from autosomes, mitochondria, and sex chromosomes and characterized the LD of the Yorkshire population, which was relatively high between closely linked markers (>0.55 at 50 kb) and declined with increasing genetic distance. The current $N_e$ of this Korean Yorkshire population was 122.87 (106.90; 138.84), while the historical $N_e$ of Yorkshire pigs suggests that the ancestor $N_e$ has decreased by 99.6% over the last 10,000 generations. Conclusion: To maintain genetic diversity of a domesticated animal population, we must carefully consider appropriate breed management methods to avoid inbreeding. Although attenuated selection can affect short-term genetic gain, it is essential for maintaining the long-term genetic variability of the Korean Yorkshire population. Continuous and long-term monitoring would also be needed to maintain the pig population to avoid an unintended reduction of $N_e$. The best way to preserve a sustainable population is to maintain a sufficient $N_e$.