• Journal of Mushroom
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• v.13 no.3
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• pp.217-222
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• 2015

This study was conducted to obtain a growth correlation of basal information from the development of disease resistant Flammulina velutipes cultivars through back-crossing between the strains of wild-type brown monokaryon 4019-20 and the derivative of commercial quality white monokaryons 3. The two strains were selected to back-cross for further enhancing their latent attributes and growth characteristics. The parents of 4019-$20{\times}M3$ back-crossed to reproduce $F_1$, M3-Sn. Using $F_1$, M3-Sn procured and isolated into 94 monokaryon strains. Further examination of growth characteristics carried out by back-crossing between M3 and $BC_1F_1$ from M3-n dikaryon. Monokaryon exhibited an irregular growth pattern and demonstrated to be sluggish development in the sawdust medium. However $BC_1F_1$(M3-n) dikaryon strains confirmed mostly regular growth pattern and demonstrated ordinary growth in the sawdust medium. The fruitbody of $BC_1F_1$ confirmed as light-brown colour to be the dominant gene. The colour distributions of fruitbody, $BC_1F_1$, resulted as follows; 7% of dark brown, 25% of brown, 27% of light brown, 16% of ivory and 25% of white. The ratio of the other color to white showed 3 to 1 which suggested two major genes were related to fruitbody color.

• Korean Journal of Environmental Biology
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• v.26 no.3
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• pp.159-169
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• 2008

This study was aimed to classify the intertidal macrozoobenthic community status after 2 years of Sea Prince oil spill, and oil spill effects along oil spreading track from heavily impacted to unaffected reference site. Field sampling was initiated in late February and continued through November 1998 seasonally, after 2.5 years of oil spill. 7 rocky sampling sites were selected among coastal regions coated and/or affected by the Sea Prince spilled oil. Identified species was 158 species, 65 family, 24 order, 9 class, 5 phylum. Mollusca was the dominant faunal group comprising 100 species (63.3%), and followed by 38 species of Crustacea (24.1%), 12 species of Echinodermata (7.6%), 5 species of Porifera (3.2%), and 3 species of Cnidaria (1.9%). On Dugpo of Sori Island, the fewest species was collected from 28 species to 35 species seasonally among sampling stations. But far away Dugpo toward Gamak Bay, the number of species increased, collecting the maximum on Sohwoenggan Island. At the wreck site of Sori Island, especially the species number of attached animals such as poriferans and anthozoans was very low compared to another site. The density and biomass on the higher tidal zone increased toward the low affected sites, but biomass on middle tidal zone decreased. The invertebrate biomass of study area was dependent on the sessile animals. The major dominant species were small-sized barnacles, Chthmallus challengeri, periwinkles, Littorina brevicula, mussels, Septifer virgatus, and so on. The biomass of C. challengeri and L. brevicula on the higher tidal zone was highest in the wreck site of Sori Island and decreased further and further. However, mussels on the middle tidal zone showed the inverse trends because of the larger individual size of mussel inhabited in Sori Island than those of another sites. As a result of community analysis, the effect of oil spill was not found distinctly. Several ecological indices and cluster analysis did not show the meaningful variation with oil track despite of the conspicuous differences among tidal heights. These indicate that the macrozoobenthic community level of oil spreaded zone recovered in some degree after the Sea Prince oil spill accident, but population or individual levels of dominant sessile animals took more recovery times.

• Korean Journal of Environmental Biology
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• v.38 no.1
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• pp.1-15
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• 2020

This study was conducted to determine whether invasive muskrats settle in the natural ecosystem of Korea. The study also aimed to provide information on the selection of target areas, basic behavioral characteristics, and habitat use-related data, all of which are essential for effective management strategy establishment and site management. To this end, a trend in international muskrat management was identified to examine the management direction according to the settlement stage. We also secured the location of 72 domestic farmers and classified five regions in Gyeonggi-do, Chungcheongnam-do, Gyeongsangbuk-do, and Sejong Special Self-governing City into regions with high potential for natural leakage to raise the need for a quick survey. We determined that muskrats successfully settled in the natural ecosystem of Korea, based on the fact that we consistently identified a muskrat habitat at the same point for three years, from 2016 to 2018, the age of the captured population was estimated to be 1.2 years, and there had been no additional make-up factors near the habitat since 2012. The muskrats that settled in Korea showed a small home range of 0.0027 ㎢ (MCP 95%) and were estimated to show regular mobility before Spring and Autumn. Also, the areas where the muskrat habitat was observed had a high level of habitat environment disturbance and their ecological and food intake characteristics reflected that disturbance. Muskrat traces were high in hydrophyte vegetation, which can be used as a hideout in open waters and where the growth of both floating leaved plants and floating plants are prevalent. Since international invasive muskrats have already settled in the domestic ecosystem and there is concern about their proliferation in the future, the government must consider a preemptive response and scientific management.

• Korean Journal of Environmental Biology
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• v.19 no.1
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• pp.7-17
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• 2001

Growth optima and tolerance ranges of marine algae species nay vary greatly during the developmental stage. The establishment stage is clearly of great importance for each generation, affecting the performance of the adult population. The present study was aimed to determine the effect of various concentrations of inorganic nutrients and heavy metals on germination and germlings of Ulva pertusa Kjellman (Chlorophyta). Percent germination increased rapidly as irradiance level increased, reaching the maximum at 100 ${\mu}$mol m$\^$-2/s$\^$-1/ in both east and west seawater. Percent germination in the east seawater was higher than that in the west seawater at irradiances lower than 30 ${\mu}$mol m$\^$-2/s$\^$-1/, and there was no difference in percent germination between the two different seawaters at irradiances higher than 60 ${\mu}$mol m$\^$-2/s$\^$-1/. Germling growth increased in the both cases with increasing irradiances but no growth was found in the dark. Overall germling area was larger in the east seawater than in the west seawater. Number of cell increased with increasing irradiance and became light-saturated at 100 ${\mu}$mol m$\^$-2/s$\^$-1/ in the both cases. Germlings grown in the east seawater had more cells than those cultivated in the west seawater at irradiances lower than 60 ${\mu}$mol m$\^$-2/s$\^$-1/. In various combinations of nitrate and phosphate, percent germination increased with increasing nitrate concentrations irrespective of phosphate concentrations. At 3 days after inoculation, nitrate concentration of 2.5 ppm with all phosphate concentrations promoted germination. Area and number of cell of germlings increased with increasing nitrate concentrations in all phosphate concentrations. In various combinations of copper and lead, germination was delayed as copper concentrations increased in all lead concentrations. Percent germination was severely reduced at 1 ppm Cu$\^$2+/. Area and number of cell of germlings decreased with increasing copper concentrations in all lead concentrations. The highest copper concentration (1 ppm) clearly suppressed the germling growth in U. pertusa.

• Korean Journal of Environment and Ecology
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• v.29 no.4
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• pp.570-579
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• 2015

Lindera sericea, which belongs to genus Lindera in the Lauraceae family, is labeled under Least Concerned (LC) among Korean rare plants, floristics specific species IV grade and also flagpole species of the Mudeung National Park. It is distributed in Mt. Mudeung, Mt. Cheonbong and Mt. Chogye within gradients from $12^{\circ}$ to $51^{\circ}$. The slope area is from northeast to northwest sides and the altitude range of distribution site is between 220 m and 533 m. The vascular plants in the quadrate where Lindera sericea were studied were identified as a total of 72 taxa; 37 families, 54 genera, 66 species, 5 variants and 1 forma. Among the floristics specific species, IV grade species was Lindera sericea and III grade species were Stewartia pseudocamellia and Acer palmatum. Korean endemic species were Stewartia pseudocamellia and Carex okamotoi. As for the vegetation group, Quercus mongolica and Q. serratak - Lindera sericea, Styrax japonicus populations were found in Mt. Mudeung area where Lindera sericea appeared, Stewartia pseudocamellia-Lindera sericea and Sasa borealis populations were found in Mt. Cheonbong areas, Carpinus laxiflora - Lindera sericea and Sasa borealis populations were found in Mt. Chogye area, and Stewartia pseudocamellia-Lindera sericea and Sasa borealis populations were found in Songgwangsa area. A total number of 662 Lindera sericea individuals were examined. The number of trees with 1 trunk including younger individuals was 353 (53.32%), and the number of trees with 2 to 5 stems was 270 (40.79%). The number of trees with the greatest number of sprouts was 27. Of 662 trees in total, the total number of sprouts was 1,198. Among these, 699 trees (58.34%) were between 50 cm and 150 cm in height. The tallest tree was 585 cm. The most common root-collar diameter of sprouts (992, 82.81%) was under 1 cm, followed by the sprouts with collar diameter from 1.0 to 1.5 cm(156, 13.2%). Among them, the largest root-collar diameter was 3.2 cm. This Lindera sericea specimen had the tallest trunk (565 cm) which was torn lengthwise on one side. Its root and breast parts were decayed by 50% and 25 respectively. Some branches of the three trunks that were more than 4 m in length were dead in the apical portion. Therefore, it is proposed that the Korean Lindera sericea maintains an apical dominance tree type while showing morphological adaptation as a typical shrub because it autonomously decays some branches and trunks over a certain height while increasing the number of its sprouts.

• Korean journal of applied entomology
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• v.25 no.2 s.67
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• pp.113-120
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• 1986

Soybeans collected from different locations in Korea were planted for tests of seed transmission of soybean mosaic virus (SMV). The percentage of seed transmission ranged from 20.5 to 29.5% in 14 seedlots including soybean cultivar Namhe and 12 to 19% in other 44 seedlots. However, no seed transmission of SMV was observed in soybean cultivar Younkiyongho. SMV was detected from embryo and cotyledon of soybean seeds. The infection of SMV was highly detected from premature seeds than from fully mature seeds, and higher from seeds harvested from plants infected before June 20 than plants infected around July 20 and August 20. No significant relationship was observed between seed transmission of SMV and mottling of seeds. The incidence of soybean mosaic disease followed by one month after peak of aphid population. The number of aphids was less on leaves of soybeans with short and dense trichomes whereas it was higher on leaves of soybean with long and sparse trichomes. Generally, the number of aphids was decreased on leaves with long and dense trichomes as the growth progressed. Soybean cultivar Columbus and 14 cultivars were susceptible, Chief and 14 cultivars were moderate, but Jangbaek and 17 other cultivars were resistant to SMV when inoculated with one isolate of SMV.

• Korean Journal of Environmental Biology
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• v.31 no.4
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• pp.322-332
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• 2013

This study was conducted to understand the control mechanism of Sika deer (Cervus nippon taiouanus) populations which are adapted to the natural forest areas and the semi-natural forest areas of Korea. We monitored and analyzed the behaviour patterns and habitat characteristics of Sika deers. After we released 12 Sika deers (male 3, female 9) each at the two study areas in and around Seoul under the approval of Korean government, we monitored them through our survey researchers' naked eyes from May 2011 to January 2012. We discriminated their habitat patterns to 4 major places - eating food place, drinking water place, daytime rest place, and nighttime rest place. Our results showed that Sika deer preferred the open grassland habitats for feeding and resting in daytime but the closed bushland for their protection and sleep. We also found the recommended sex ratio of adult male to female in a herd was 1 : 4~9 for breeding and they had few cubs in a herd. The average amount of food for 1 adult deer was 391.62 kg of fresh grasses and 286.90 kg of hay per year. Our results suggest that the amount of drinking water required for a deer when grazed on grasses during spring and summer seasons is 256.8 L and when a deer feeds on dry food during autumn and winter seasons, 209.8 L of water is required. From these results, we observe that the amount of drinking water was affected substantially by the moisture content of their food. This observational study must be very valuable for land managers to judge the number of deers that should be released in natural areas without high risk and to ascertain the sex ratio of large mammals including Sika deer. Therefore, we conclude that this population control mechanism study consists of efficient basic data and their values must be very effective for various purposes.

• Journal of the Korean Applied Science and Technology
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• v.36 no.2
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• pp.394-406
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• 2019

The cellular senescence may be due to damage by the reactive oxygen species (ROS). This study has compared the antioxidant activity in the human cell lines of various origins, including 10S and 50S-derived normal skin fibroblasts, and 10S bone marrow, dental tissue and adipose-derived adult stem cells. After being exposed to $H_2O_2$, half inhibitory concentration ($IC_{50}$) values by cytotoxicity assay was significantly (P<0.05) lower in 50S-derived skin fibroblasts, than in 10S-derived skin fibroblasts and various adult stem cell lines. The cell population doubling time (PDT) and the cell frequency with high senescence associated-${\beta}$-galactose activity were remarkably increased in 50S-derived fibroblasts exposed to 50 ppm $H_2O_2$ for 7 days, than those of 10S-derived fibroblasts and various adult stem cell lines. Further, the expression level of antioxidant-related genes, glutathione peroxidase (GPX) and catalase (CAT), was investigated in 10S and 50S-derived skin fibroblasts, and 10S-derived various adult stem cells by reverse transcription polymerase chain reaction (RT-PCR). The expression level of GPX was higher in most of cell lines, compared to CAT, and a significantly (P<0.05) higher expression level of GPX was observed in 10S-derived skin fibroblasts and adult stem cell lines, compared to 50S-derived skin fibroblasts. We concluded that old-aged skin fibroblasts seemed to be less resistant against ROS than young-aged skin fibroblasts and adult stem cells.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.19 no.1
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• pp.12-19
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• 2019

Purpose: Isolated methylmalonic acidemia (MMA) is an autosomal recessive inherited disorder of propionate metabolism. There are two subtypes of MMUT gene defects. $Mut^0$ represents complete loss of methylmalonyl-CoA mutase (MCM) activity while mut- is associated with residual MCM activity, which can be stimulated by hydroxocobalamin (OHCbl) supplementation. The objective of this study is to investigate cobalamin responsiveness and mutations present in Korean MMA population. Methods: We evaluated 10 MMA patients using somatic cell complementation analysis on their fibroblasts to measure MCM activity and vitamin B12 responsiveness for the optimal treatment. MMUT gene was sequenced to identify the MMA mutations. Results: For all patients, the incorporation of $[^{14}C]-propionate$ was low, and there was no response to OHCbl. The incorporation of $[^{14}C]-methyltetrahydrofolate$ and $[^{57}Co]-CNCbl$ fell within the normal range. There was adequate synthesis of methylcobalamin while the synthesis of adenosylcobalamin was low. The complementation analysis showed all patients were $mut^0$. The sequence analysis identified 12 different MMUT mutations, including 2 novel mutations, p.Gln267Ter and p.Ile697Phe, were identified. All the patients in this study had neonatal onset of symptoms, belonged to $mut^0$ complementation class, and as a result, showed no cobalamin responsiveness. Conclusion: No Korean MMA patient showed cobalamin responsiveness.

• Journal of Life Science
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• v.29 no.6
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• pp.724-734
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• 2019

The present study investigated the cytotoxicity of particulate matter (PM) derived from car air filter (outdoor PM) and home cleaner filter (indoor PM) in the various human cell lines. Each outdoor and indoor PM were harvested by ethanol extraction method, subsequently sieved with 10 um filter paper, sterilized with autoclave and added to culture media. The half maximal inhibitory concentration ($IC_{50}$) values was significantly (p<0.05) lower in the outdoor PM, compared with indoor PM, and the significantly (p<0.05) higher $IC_{50}$ values were observed in the cancer cell lines (A-549 lung adenocarcinoma and AGS stomach adenocarcinoma), than those of normal MRC-5 fibroblasts and dental papilla tissue derived-mesenchymal stem cells (DSC). After being exposed to $100{\mu}g/ml$ outdoor PM for 7 days, the population doubling time (PDT) was significantly (p<0.05) increased in especially MRC-5 and DSC cell lines, compared with untreated cell lines. Further, the expression of senescence-associated ${\beta}$-galactosidase activity was up-regulated in all the cells exposed to outdoor PM than those of untreated control. Besides, the expression level of inflammation-associated genes, such as cyclooxygenase-2 (COX-2) and interleukin-6 (IL-6) was found to be significantly (p<0.05) increased in the outdoor PM-treated cell lines than those of untreated cell lines. Our results showed that PM induces the cytotoxicity via arrest of cell growth, cell damage and inflammation response.