• 한국발생생물학회지:발생과생식
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• 제17권4호
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• pp.379-387
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• 2013

Embryonic stem (ES) cells can self-renew and differentiate to various cells depending on the culture condition. Although ES cells are a good model for cell type specification and can be useful for application in clinics in the future, studies on ES cells have many experimental restraints including low transfection efficiency and transgene expression. Here, we observed that transgene expression after transfection was enhanced by treatment with histone deacetylse (HDAC) inhibitors such as trichostatin A, sodium butyrate, and valproic acid. Transfection was performed using conventional transfection reagents with a retroviral vector encoding GFP under the control of CMV promoter as a reporter. Treatment of ES cells with HDAC inhibitors after transfection increased population of GFP positive cells up to 180% compared with untreated control. ES cells showed normal expression of stem cell markers after treatment with HDAC inhibitors. Transgene expression was further enhanced by modifying transfection procedure. GFP positive cells selected after transfection were proved to have the stem cell properties. Our improved protocol for enhanced gene delivery and expression in mouse ES cells without hampering ES cell properties will be useful for study and application of ES cells.

• Asian-Australasian Journal of Animal Sciences
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• 제29권6호
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• pp.782-792
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• 2016

As the most common neurodegenerative diseases, Alzheimer's disease (AD) and Parkinson's disease (PD) are two of the main health concerns for the elderly population. Recently, microRNAs (miRNAs) have been used as biomarkers of infectious, genetic, and metabolic diseases in humans but they have not been well studied in domestic animals. Here we describe a computational biology study in which human AD- and PD-associated miRNAs (ADM and PDM) were utilized to predict orthologous miRNAs in the following domestic animal species: dog, cow, pig, horse, and chicken. In this study, a total of 121 and 70 published human ADM and PDM were identified, respectively. Thirty-seven miRNAs were co-regulated in AD and PD. We identified a total of 105 unrepeated human ADM and PDM that had at least one 100% identical animal homolog, among which 81 and 54 showed 100% sequence identity with 241 and 161 domestic animal miRNAs, respectively. Over 20% of the total mature horse miRNAs (92) showed perfect matches to AD/PD-associated miRNAs. Pigs, dogs, and cows have similar numbers of AD/PD-associated miRNAs (63, 62, and 59). Chickens had the least number of perfect matches (34). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses suggested that humans and dogs are relatively similar in the functional pathways of the five selected highly conserved miRNAs. Taken together, our study provides the first evidence for better understanding the miRNA-AD/PD associations in domestic animals, and provides guidance to generate domestic animal models of AD/PD to replace the current rodent models.

• 대한치과교정학회지
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• 제42권6호
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• pp.307-317
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• 2012

Objective: The purpose of this study was to investigate the isolation and characterization of multipotent human periodontal ligament (PDL) stem cells and to assess their ability to differentiate into bone, cartilage, and adipose tissue. Methods: PDL stem cells were isolated from 7 extracted human premolar teeth. Human PDL cells were expanded in culture, stained using anti-CD29, -CD34, -CD44, and -STRO-1 antibodies, and sorted by fluorescent activated cell sorting (FACS). Gingival fibroblasts (GFs) served as a positive control. PDL stem cells and GFs were cultured using standard conditions conducive for osteogenic, chondrogenic, or adipogenic differentiation. Results: An average of $152.8{\pm}27.6$ colony-forming units was present at day 7 in cultures of PDL stem cells. At day 4, PDL stem cells exhibited a significant increase in proliferation (p < 0.05), reaching nearly double the proliferation rate of GFs. About $5.6{\pm}4.5%$ of cells in human PDL tissues were strongly STRO-1-positive. In osteogenic cultures, calcium nodules were observed by day 21 in PDL stem cells, which showed more intense calcium staining than GF cultures. In adipogenic cultures, both cell populations showed positive Oil Red O staining by day 21. Additionally, in chondrogenic cultures, PDL stem cells expressed collagen type II by day 21. Conclusions: The PDL contains multipotent stem cells that have the potential to differentiate into osteoblasts, chondrocytes, and adipocytes. This adult PDL stem cell population can be utilized as potential sources of PDL in tissue engineering applications.

• 한국습지학회지
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• 제17권1호
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• pp.19-25
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• 2015

본 연구에서는 정수 환경에 정착한 3생활형 9분류군의 식물을 대상으로 1년동안 정기적으로 모니터링을 실시하였으며, 이를 통해 식물 생활형이 수위 조건에 따라 식물 정착에 어떠한 영향을 미치는지 밝히고자 하였다. 18개의 실험구를 셋으로 나누어 16주 범람 기간 동안 0, 20, 60cm로 수위를 각각 유지하였으며, 범람 기간을 제외한 기간은 0cm로 수위를 모두 동일하게 유지하였다. 1차년에 식재한 다년생 식물인 큰고랭이(Scirpus tabernaemontani), 줄(Zizania caduciflora), 부들속 2종(Typha angustifolia, T. orientalis)은 종자에 의한 추가적인 정착은 없었으며, 수문 조건이 밀도 및 초고 생장에 큰 영향을 미치지 않았다. 그러나 1년생 식물인 고마리(Persicaria thunbergii), 여뀌(Persicaria hydropiper), 사마귀풀(Aneilema japonicum)과 2년생인 벼룩나물(Stellaria uliginosa), 뚝새풀(Alopecurus aequalis), 개피(Beckmannia syzigachne)는 상대적으로 수문 조건에 의한 영향을 크게 받았다. 정수 환경에서 침수 수위 및 침수 기간은 상대적으로 지하경을 형성하지 못하는 1, 2년생 식물 종들의 정착 및 생육에 큰 영향을 미치는 것으로 보인다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권5호
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• pp.2499-2506
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• 2016

Background: Gastric cancer (GC) is the second leading cause of cancer-related death worldwide. Several environmental, genetic and epigenetic factors have been suggested to have a role in GC development. Epigenetic mechanisms like histone changes and promoter hyper-methylation are now being increasingly studied. Associations between methylation of many gene promoters with the risk of gastric cancer have been investigated worldwide. Such aberrant methylation may result in silencing of specific genes related to cell cycling, cell adhesion, apoptosis and DNA repair. Thus this molecular mechanism might have a key role in proliferation and migration of cancerous cells. Materials and Methods: In this review article we included studies conducted on DNA methylation and gastric cancer in Iranian populations. Using Science direct, Pubmed/PMC, Springer, Wiley online library and SciELO databases, all published data until 31 January 2016 were gathered. We also searched Science direct data base for similar investigations around the world to make a comparison between Iran and other countries. Results: By searching these databases, we found that the association between methylation of seven gene promoters and gastric cancer had been studied in Iran until 31 January 2016. These genes were p16, hLMH1, E-cadherin, CTLA4, $THR{\beta}$, mir9 and APC. Searching in science direct database also showed that 92 articles had been published around the world till January 2016. Our investigation revealed that despite the importance of GC and its high prevalence in Iran, the methylation status of only a few gene promoters has been studied so far. More studies with higher sample numbers are needed to reveal the relation of methylation status of gene promoters to gastric cancer in Iran. Conclusions: Further studies will be helpful in identifying associations of DNA methylation in candidate genes with gastric cancer risk in Iranian populations.

• Animal Systematics, Evolution and Diversity
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• 제17권1호
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• pp.1-11
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• 2001

동아시아의 계류와 하천에 우점적으로 서식하는 딱정장수노벌레속 Canthocamptus (요각아강, 갈고리노벌레목)의 mirabilis 종군은 Attheyella속과의 중간 형질을 나타내는 특이한 분류군으로서, 이소적 또는 측소적으로 매우 빠르고 다양하게 종분화한 것으로 최근 연구되고 있다. 그러나, 대표종이라 할 수 있는 C. mirabilis Sterba가 매우 불완전하게, 그것도 암컷만으로 기재되었고 모식이 남아있지 않아서 이 종군의 분류에 많은 지장과 혼란이 있어 왔다. 저자는 중국 북경 근교의 모식산지에서 새롭게 채집한 표본을 근거로 재기재를 하였고, 이를 통해 앞서 일본산 표본을 근거로 하여 작성되었던 과거의 재기재와의 차이점을 열거함으로써 일본산 표본들은 별개의 종임을 제시하였다. 아울러 본 종군에 속하는 여하 종과의 형태적 차이와 유연관계에 대하여서도 간략히 고찰하였다.

• Animal Systematics, Evolution and Diversity
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• 제18권2호
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• pp.233-244
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• 2002

딱정장수노벌레 (Canthocamptus)속의 mirabilis 종군에 대한 분류학적 연구의 일환으로. 극동아시아의 남부에 널리 분포하는 반달딱정장수노벌레 (C. semicircuius Kikuchi)와 남한의 중서부 지역의 구릉지대 계류에 분포하는 1신종. 고려딱정장수노벌레 (C. coreensis)를 보고한다 반달딱정장수노벌레의 한국산 표본에서는 암컷의 바깥꼬리털과 수컷의 제3다리 외지의 돌기형 가시와 같은 중요 형질에서 변이가 흔하게 나타났기에 종내변이에 대해 특기하였다. 고려딱정장수노벌레는 암컷에서 수컷모사변이형의 꼬리마디가 나타나지 않고 항문판의 투명막이 둥근모양을 유지한다는 점 등의 조상형 형질을 유지하는 반면. 수컷 제3다리 외지 제2마디의 바깥가시와 수컷 제4다리 외지 끝마디 바깥강모가 심하게 변형되는 등의 파생형질을 소유한다. 현재까지 기록된 5종의 한국산 딱정장수노벌레속 mirabilis 종군에 대한 종검색표를 작성하였다.

• 한국약용작물학회지
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• 제13권5호
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• pp.213-218
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• 2005

Phellinus linteus (PL), one of the immune-regulatory substances, is recognized to play the role in the metabolic process on inflammation and immunity. It has been traditionally used in the oriental medicine to treat inflammatory related disease. The purpose of this study was to evaluate the effects of water extracts of PL on the mesenteric lymph node lymphocytes immune function in the ICR male mice. Control mice received vehicle only. The PL treated mice were administered the respective extract by oral gavages for 4 weeks. IgE concentrations in serum and MLN lymphocytes were significantly lower in PL treated mice than in control mice. PL increased the proportion of $CD4^+\;and\;CD8^+$ T cells in MLN lymphocytes. PL significantly decreased Th2 cytokine concentrations and mRNA expression levels in cytokine secretions. Therefore, water extracts of PL modulate inflammatory parameters through regulation of immunoglobulin production resulting from decreased Th2 cytokine secretion and mRNA expression levels and reduce pro-inflammatory cytokine secretion and mRNA expression in MLN lymphocytes.

• The Korean Journal of Ecology
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• 제12권1호
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• pp.51-65
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• 1989

Some aspects of limno-biology of the five dammed lakes such as Tamyang, Changsong, Kwangju, Naju, and Youngsan Lake along the Yongsan River were investigated from June 1986 to July 1988 for the comparison on the lentic ecosystems in relation to the fish fauna. Sixty four fish species representing 48 genera and 21 families of fishes were collected in these dammed areas, which included 29 species of the family Cyprinidea and 40 speaies of the primary fresh-water fishes. Among, these, 12 species such as Rhodeus Acheilognothus yamatsutae, A canathorhodeus gracilis, Sarcocheilichthys nigripinis morii, S. variegatus wakiyae, Gnathopogon strigatus, Squalidus gracilis majimae, Microphysogobio yaluensis, Cobitis longicorpus sp, Liobagrus mediadiposalis and Odontobutis platycephala are enddmic to Korea. Leiocassis nitidus and Gasterosteus aculeatus which occur in these water areas take note of the zooge rographic study of Korea. The fish species in each of the dammed lakes is in proportion of the scale of the lakes such as 32 species in Thamyang, 40 species in Changsong, 24 species in Kwangju, 35 species in Jaju, and 52 species in Yongsan Lake. The dominant fish species of the 4 upper dammed lakes of Thamyang, Changsong, Kwangju and Naju Lake are Zacco platypus and Rhodeus uyekii, while those of Youngsan Lake are Hemiculter eigenmanni and Carassius auratus. This difference of fish species of those areas suggests that the upper damed lake differ from the lower dammed lakes in the habitable condition of fishes. The migratory fish species have significantly declined and will continue to decline in both population size and number of species in the dammed lakes in near future as a result of the dam construction barriers in the river estuary. The major migratory fishes of this water area are as follows; Anguilla japonica, Plecoglossus altivelis, Hypomesus olidus, Gasterosteus aculeatus, Konosirus Punctatus, Coilia ectens, Hemirhampus sajori, Mugil cephalus. Acanthogobius flavimanus, and Takifugu ocellatus.

• 환경정책연구
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• 제10권3호
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• pp.73-91
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• 2011

2000년대 초반의 동아시아 수산활동을 기후변화와 사회경제적 개발과 연관하여 연구하였다. 세계 인구의 약 1/3이 동아시아 지역에 살고 있는데, 이들은 많은 수산물을 생산하고, 소비하며, 국제무역에 기여하고 있다. 지역적으로, 전지구적으로 진행되고 있는 기후변화와 더불어 해수의 온난화 및 산성화는 수산생물과 수산업에 심대한 영향을 미칠 것이다. 동아시아 지역의 잦은 태풍은 연안의 사회집단에 막대한 손실과 희생을 유발할 것이며, 이러한 환경의 변화속도는 우리가 효과적으로 대응하기에 벅찰 정도로 빠르게 진행 중이다. 과학적 활동은 기후변화의 상황에서 발생하는 문제점들을 찾아내고, 더 나아가 효과적인 해결책을 만들기 위한 기본 지침을 제공하여야 한다. 또한 수산관리 계획은 기후변화와 더불어 사회체제의 변화를 고려하여 수립되어야 한다. 그러므로 이 해역에서 공동과학연구를 추진하고, 수산자원을 보존하고 관리하는 국제적 공조체제를 구축하여야 한다는 제안은 매우 논리적이다.