• 제목/요약/키워드: polysaccharide-7

검색결과 370건 처리시간 0.034초

큰눈물버섯 (Psathyrella velutina)으로부터 분리한 단백다당체 PVP의 항암 . 면역활성 (Antitumor Immunomodulatory Activity of PVP a Protein-polysaccharide Fraction Prepared from a Wild Mushroom Psathyrella velutina)

  • 정경수;이지선
    • 약학회지
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    • 제45권6호
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    • pp.617-622
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    • 2001
  • A protein-polysaccharide fraction of a Korean wild mushroom Psathyrella velutina, PVP, was prepared and its antitumor immunomodulatory activity was investigated. When PVP was administered once daily for seven days from day 1 to day 7 into male ICR mice implanted with 1 $\times$ 10$^{5}$ cells of sarcom 180 tumor cells into the peritoneum on day 4, it inhibited the growth of sarcoma 180 cells by 92.8%. In XTT assay, PVP also exhorted in vitro anti-proliferation activity on U-937, a human monoblastoid cell line, as well as sarcoma 180 cells. PVP showed marked stimulatory activity on the immune system in that it induced the accumulation of PEC (the stimulation index, Sl=4.90 at 100 mg/kg), stimulated the BALB/c mouse splenic lymphocytes to form lymphoblasts (Sl=5.75 at 100$\mu\textrm{g}$/ml), and upergulated the expression of CD25 molecules. All these results strongly support that PVP exhorts its antitumor activity through stimulation of the immune system as well as anti-proliferative activity on the tumor cells.

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Biopolymer 생산세균의 분리 및 증식패턴 (Isolation of Biopolymer-producing Bacterium and Its Growth Pattern)

  • Yoo, Jin-Young;Chung, Dong-Hyo
    • 한국미생물·생명공학회지
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    • 제17권5호
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    • pp.421-428
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    • 1989
  • 물성조정제로서 고전도의 다당류를 생산하는 토양세균을 분리 선발하여 동정한 결과 Pseudomonas delafieldii로 판단되었다. 이 세균의 증식을 위한 pH와 온도는 6.5 및 3$0^{\circ}C$이었다. 최대 비성장속도는 0.25 h$^{-1}$이며 염류배지에서 다당류의 비생산속도, 증식수율 및 다당류 생산수율은 6.25mg/g-cell/h, 54.5%, 38.39%이었다. 본 다당류는 포도당과 gluconolactone을 몰비로 1.9:1.0로 함유한 $\beta$-glucan 으로 추정되었으며 원인 분석결과 탄소 31.85% 수소 31.85% 수소 5.l5%를 함유하고 아세틸기를 1.35%가지고 있었으며 고유점도는 42.84dl/g, 분자량은 5.64$\times$$10^7$이었다.

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Antitumor Constituents of Polyporus giganteus

  • Kim, Byong-Kak;Shim, Mi-Ja;Kim, Ha-Won;Woo, Myoung-Sik;Choi, Eung-Chil
    • Archives of Pharmacal Research
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    • 제10권3호
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    • pp.179-183
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    • 1987
  • To investigate antitumor constituents of higher fungi, the carpophores of Polyporus giganteus Pers. ex. Fr. (81 g, dry weight) which were collected in Indiana, U. S. A. were examined for antitumor activity. Two protein-bound-polysaccharide fractions (I and II) were prepared from the hot water extract and one fraction (III) from the 0.1 N NaOH extract of the carpophores. The antitumor effect of each fraction was tested against sarcoma 180 implanted subcutaneously in female ICR mice. Of three fractions, Fraction II showed 85.2% inhibition ratio at the dose of 20 mg/kg/day for 10 days and was named gigantan. Gigantan was found to contain 59% polysaccharide and 27% protein. Its polysaccharide moity was a heteroglycan that consisted of mainly glucose (89.3%), galactose (7.7%), minaose (2.0%) and fructose (1.0%).

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홍색 비황 광합성 세균 Rhodopseudomonas sp. KH4의 Extracellular polysaccharide의 중금속 흡착 (Adsorption of Heavy Metal onto the Extracellular Polysaccharide Produced by the Purple Nonsulfur Photosynthetic Bacteria Rhodopseudomonas sp. KH4)

  • 정정화;서필수;공성호;이종렬;이상섭
    • 미생물학회지
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    • 제42권4호
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    • pp.326-331
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    • 2006
  • 본 연구에서는 경기도 안양에 있는 하천에서 분리한 홍색 비황 광합성 세균Rhodopseudomonas sp. KH4에서 생성된 extracellular polysaccharide (EPS)의 중금속 흡착특성에 대해 연구하였다. 100 mg/L의 Cd과 Cu의 최적 흡착조건(온도$40^{\circ}C$ Cd; pH 8, Cu; pH 5)에서 1.0 g/L의 EPS를 첨가하였을 때, Cd의 경우 30분 동안 842 mg/L, Cu의 경우 10분 동안 70.0 mg/L가 흡착되었다. 또한 Cd과 Cu가 각각 100 mg/L씩 공존되어 있는 경우, pH 5, $25^{\circ}C$에서 EPS 1.0 g/L 첨가하였을 때 Cd과 Cu가 각각 16.8 mg/L, 48.7 mg/L가 흡착되었다. 또한 Langmuir 등은 흡착식으로 부터 EPS 1.0 g/L, pH 5, $25^{\circ}C$에서 Cd과 Cu의 흡착량은 각각 76.9 mg/L, 67.1 mg/L로 실제 경험치와 큰 차이를 보이지 않았다. 에탄올 침전법으로 정제된 KH4 strain으로부터 생성된 EPS는 gas-liquid chromatography (GLC) 분석에 의한 중성당 분석 결과, arabinose, glucose, mannose가 각각 2.4%, 7.1%, 90.5%로 mannose가 대부분을 구성하였다.

브로콜리로부터 분리한 추출물의 In vitro 면역증진 활성평가 및 화학적 특성 (Chemical Properties and Assessment of Immunomodulatory Activities of Extracts isolated from Broccoli)

  • 곽봉신;박혜령;이수정;최혁준;신광순
    • 한국식품영양학회지
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    • 제30권6호
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    • pp.1140-1148
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    • 2017
  • For the purpose of developing new immunomodulatory agents from broccoli, ethanol extract (BCEE), hot water extract (BCHW), and crude polysaccharide (BCCP) were isolated from broccoli, and their immunomodulatory activities and chemical properties were examined. In the in vitro cytotoxicity analysis, BCHW and BCCP did not affect the growth of tumor cells and normal cells. Murine peritoneal macrophages stimulated with BCCP showed higher production of IL-6, IL-12, and $TNF-{\alpha}$ cytokines than those stimulated with BCHW. Also, BCHW and BCCP did not show proliferation of splenic lymphocytes. In the in vitro assay for intestinal immunomodulatory activities, only BCCP enhanced GM-CSF secretion and the bone marrow cell-proliferating activity via cells in Peyer's patches at $1,000{\mu}g/mL$. Also, BCHW mainly contained 33.7% neutral sugars, such as arabinose, glucose, and galactose, and 30.7% uronic acid, and BCCP consisted of 42.6% neutral sugars, including arabinose, galactose, and glucose, and 50.5% uronic acid. The above results lead us to conclude that crude polysaccharide (BCCP) isolated from broccoli causes considerably high cytokine production in peritoneal macrophages and bone marrow cell proliferation, and the polysaccharide extraction process is indispensable for separation of new immunomodulatory agents from broccoli.

턱수염 버섯 배양(培養) 균사(菌絲)의 항종양(抗腫瘍) 성분(成分) (An Antitumor Constituent of the Cultured Mycelia of Hydnum repandum)

  • 정경수;최응칠;김병각;김양섭
    • 한국균학회지
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    • 제11권2호
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    • pp.91-95
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    • 1983
  • 한국산 담자균류의 항종양성 성분을 탐색하기 위하여 턱수염버섯의 균사를 분리하여 인공배지에서 진탕배 양후, 그 배양균사체로부터 수용셩 단백성 다당류를 추출하였다. 이 성분이 ICR마우스에 이식한 육종 180에 대하여 54.3%의 종양억제율을 나타내었다. 이 성분은 다당류 42%와 단백질 28%로 구성되어 있었다. 그 중 다당류는 glucose (57.8%), mannose(19.3%), galactose(10.8%), xylose (6.8%), 및 fucose (5.7%)를 함유하고 있었다.

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Bacillus sp. A29에 의한 다당류의 생산과 물성 (Production and Rheological Properties of the Polysaccharide from Bacillus sp. A29)

  • 안성구;서현호;이창호;오희목;권기석;이동희;윤병대
    • 한국미생물·생명공학회지
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    • 제22권2호
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    • pp.175-181
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    • 1994
  • A bacterial strain producing highly viscous polysaccharide(A29 POL) was isolated from soil and identified sa Bacillus sp. A29. The cultural conditions of the Bacillus sp. A29 for the polysaccharide prouction were dextrin 12%, soytone 0.2%, SnCl$_{2}$ $\cdot $2H$_{2}$O 0.02%, Na$_{2}$HPO$_{4}$ $\CDOT $12H$_{2}$O 0.36%, L-alanine 0.01%, initial pH6.8, and 30$\circ $C at pH 3 FOR 4 days. Final viscosity of the culture broth was 65, 000 cp and then the amount of produced polysaccharide was 8.3 g/l. A29 POL was composed of glucose and xylose. A29 POL showed high viscosity at low concentration(0.1%) and in the presence of the salts such as NaCl or CaCl$_{2}$. A29 POL showed high viscosity acid condition and at alkali condition and high pseudoplasticity in the presence of a NaCl or CaCl$_{2}$. It was shown that the viscosity at high temperature(80$\circ $C) was decreased but it was recovered at low temperature (20$\circ $C. A29 POL was able to from film and gel in the presence of MgSO$_{4}$ $\CDOT $7H$_{2}$O, Na$_{2}$CO$_{3}$ \CDOT $H$_{2}$O, MnSO$_{4}$ $\CDOT $ 7H$_{2}$O. A29 POL had anionic charge.

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Polysaccharide Degrading Enzyme을 이용한 참모자반 효소분해 추출물의 생리활성 연구 (Biological Analysis of Enzymatic Extracts from Sargassum fulvellum Using Polysaccharide Degrading Enzyme)

  • 조은경;강수희;최영주
    • KSBB Journal
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    • 제28권6호
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    • pp.349-355
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    • 2013
  • SC092 strain, producing a polysaccharide degrading enzyme, was isolated from the seawater. This strain was identified as Microbulbifer sp. using the comparative sequence analysis against known 16S rRNA sequence. A polysaccharide degrading enzyme from this strain was used to acquire the enzymatic extracts of Sargassum fulvellum. DPPH radical scavenging and SOD activity of the enzyme extracts of S. fulvellum were about 61.9% and 82.9% at 2 mg/mL, respectively. Nitrite scavenging activities was 52.5% at 2 mg/mL on pH 1.2. In addition, ${\alpha}$-glucosidase inhibitory activity was also increased in a dose-dependent manner and was about 52.7% at 2 mg/mL. To determine the influence of enzyme extracts of S. fulvellum on alcohol metabolism, the generating activity of reduced-nicotinamide adenine dinucleotide (NADH) by alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) were measured. ADH and ALDH activities were 118.0% and 177% at 2 mg/mL, respectively. ${\alpha}$-glucosidase inhibitory activity of enzyme extracts of S. fulvellum was remarkably increased in a dose-dependent manner and was about 52.7% at 2 mg/mL. These results indicate alcoholizing and ${\alpha}$-glucosidase inhibitory activities can be enhanced by the enzymatic extracts of S. fulvellum.

군소(Aplysia kurodai)에서 추출한 다당 분획물의 면역 조절 효과 (Immune Regulating Effect of Polysaccharide Fraction from Sea Hare (Aplysia kurodai))

  • 박시향;정세영;최영준
    • 한국식품영양과학회지
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    • 제40권3호
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    • pp.372-378
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    • 2011
  • 본 실험에서는 군소로부터 다당류를 추출 정제한 다당 분획물을 혈액 림프구와 대식 세포주를 사용하여 면역 조절효과를 실험해 보았다. 군소부터 추출 분획한 다당류는 48시간 동안 처리 시 Jurkat cell의 증식률을 40% 이상 증가시켰으며, 혈액암 세포종인 Jiyoye cell에 대하여는 그 성장률이 농도에 따라 감소하였다. 그렇지만 Jurkat cell에 24시간과 48시간 동안 군소 다당 분획물을 처리하였을 때 IL-2와 IFN-$\gamma$ 생성량의 유의적인 증가는 확인할 수 없었다. 그러나 RAW264.7 cell line에 대하여는 IL-12의 경우는 47% 이상 증가하여, 군소 다당 분획물의 면역 조절 효과의 가능성을 보여주었다.

Effects of Selenizing Codonopsis pilosula Polysaccharide on Macrophage Modulatory Activities

  • Qin, Tao;Ren, Zhe;Lin, Dandan;Song, Yulong;Li, Jian;Ma, Yufang;Hou, Xuehan;Huang, Yifan
    • Journal of Microbiology and Biotechnology
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    • 제26권8호
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    • pp.1358-1366
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    • 2016
  • The purpose of the present study was to investigate the immune-enhancing activity of selenizing Codonopsis pilosula polysaccharide (sCPPS5) in nonspecific immune response. In in vitro experiment, the results showed that sCPPS5 could promote the phagocytic uptake, NO production, and TNF-α and IL-6 secretion of RAW264.7 cells. sCPPS5 could also strongly increase the IκB-α degradation in the cytosol and the translocation of NF-κB p65 subunit into the nucleus of RAW264.7 cells. In the vivo experiment, sCPPS5 at medium doses could significantly improve the phagocytic index of peritoneal macrophages and induce the secretion of TNF-α and IL-6. Moreover, the effect of sCPPS5 was significantly better than Codonopsis pilosula polysaccharide (CPPS). These results indicated that selenylation modification could significantly enhance the immune-enhancing activity of CPPS in the nonspecific immune response.