Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Immune Regulating Effect of Polysaccharide Fraction from Sea Hare (Aplysia kurodai)

군소(Aplysia kurodai)에서 추출한 다당 분획물의 면역 조절 효과

  • Park, Si-Hyang (Dept. of Seafood Science and Technology/ Institute of Marine Industry, Gyeongsang National University) ;
  • Choung, Se-Young (Dept. of Hygienic Chemistry, College of Pharmacy, Kyung Hee University) ;
  • Choi, Yeung-Joon (Dept. of Seafood Science and Technology/ Institute of Marine Industry, Gyeongsang National University)
  • 박시향 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 정세영 (경희대학교 약학대학 위생약학 및 독성학실) ;
  • 최영준 (경상대학교 해양식품공학과/해양산업연구소)
  • Received : 2011.01.24
  • Accepted : 2011.02.18
  • Published : 2011.03.31
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Abstract

We extracted polysaccharide from the sea hare, Aplysia kurodai, purified it partially, and experimented its immune response using the human blood lymphocytes and macrophage cell lines. Aplysia kurodai polysaccharide fraction (APF) improved the growth of the T cell (Jurkat) up to 40% by treatment for 48 hours, and decreased the growth of blood cancer, Jiyoye cell line. The APF on RAW 264.7 cell also increased interleukin-12 up to 47%. In contrast, the secretion of interleukin-2 and interferon-gamma by treatment of only APF or APF and concanavalin A on Jurkat for 24 hours and 48 hours didn't influence significantly. These results suggest that the APF has possible immune regulating ability.

본 실험에서는 군소로부터 다당류를 추출 정제한 다당 분획물을 혈액 림프구와 대식 세포주를 사용하여 면역 조절효과를 실험해 보았다. 군소부터 추출 분획한 다당류는 48시간 동안 처리 시 Jurkat cell의 증식률을 40% 이상 증가시켰으며, 혈액암 세포종인 Jiyoye cell에 대하여는 그 성장률이 농도에 따라 감소하였다. 그렇지만 Jurkat cell에 24시간과 48시간 동안 군소 다당 분획물을 처리하였을 때 IL-2와 IFN-$\gamma$ 생성량의 유의적인 증가는 확인할 수 없었다. 그러나 RAW264.7 cell line에 대하여는 IL-12의 경우는 47% 이상 증가하여, 군소 다당 분획물의 면역 조절 효과의 가능성을 보여주었다.

Keywords

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