• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1978.10a
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• pp.208.4-209
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• 1978

The Chitinase which hydrolyzes the chitin, $\beta-1,$ 4-polymer of N-acetyl glucosamine, was purified from the culture broth of Streptomyces sp. 115-5 strain. The homogeneity of enzyme was reveali by CM-Sephadex C-50 column chromatography and polyacrylamide gel electrophoresis. The purified enzyme hydrolyzed chitin and chitosan, but not cellulose. And with chitin as the substrate, a Km value of 3.6mg per ml and a Vmax of $100\mu$ mole per hr were found. The activation energy for the reaction was 3.66 Kcal per mole. The M. W. was estimated 56,000 daltons, and PI as 3.0. The chitinase was inhibited by the addition of glucose, glucuronic acid, sorbitol and xylose as product inhibitors and its inhibition pattern by glucose was estimated pure competitive type.

• Structural Engineering and Mechanics
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• v.46 no.1
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• pp.111-135
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• 2013

An experimental study has been carried out on square plain concrete (PC) and reinforced concrete (RC) columns strengthened with carbon fiber-reinforced polymer (CFRP) sheets. A total of 78 specimens were loaded to failure in axial compression and investigated in both axial and transverse directions. Slenderness of the columns, number of wrap layers and concrete strength were the test parameters. Compressive stress, axial and hoop strains were recorded to evaluate the stress-strain relationship, ultimate strength and ductility of the specimens. Results clearly demonstrate that composite wrapping can enhance the structural performance of square columns in terms of both maximum strength and ductility. On the basis of the effective lateral confining pressure of composite jacket and the effective FRP strain coefficient, new peak stress equations were proposed to predict the axial strength and corresponding strain of FRP-confined square concrete columns. This model incorporates the effect of the effective circumferential FRP failure strain and the effect of the effective lateral confining pressure. The results show that the predictions of the model agree well with the test data.

• Journal of the Korean Applied Science and Technology
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• v.17 no.2
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• pp.149-156
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• 2000

For the adsorption of polyelectrolyte at the surface of polyacrylamide gel particle, preferential adsorption of the large polyelectrolyte such as DNA is governed by the surface area of an adsorbent. The adsorption equilibrium constant can be varied by surface geometry of porous polymer, and it can be described as a function of ionic strength and surface area. Physical parameters affecting the adsorption were estimated using the theoretical governing equation of polyelectrolyte which electrophoretically moved along the column, and geometrical surface area was estimated by Waldman-Mayer's physical model. The separation of polyelectrolytes was studied using the physical parameters estimated by ionic strength and surface geometry.

• Steel and Composite Structures
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• v.17 no.4
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• pp.371-386
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• 2014

This paper presents a tri-linear restoring force model based on the test results of 12 circular RC columns strengthened by CFRP strips under low cyclic loading. The pre-stress of CFRP strips and axial load ratio of specimens are considered as the affect parameters of the proposed model. All essential characteristics of the hysteretic behavior of the proposed model, including the hysteretic rules, main performance points, strength degradation, stiffness degradation and confinement effects are explicitly analyzed. The calculated results from the proposed model are in good agreement with the experimental results, which shows that the recommended model can be reliably used for seismic behavior predictions of circular RC columns strengthened by pre-stressed CFRP strips.

• Steel and Composite Structures
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• v.14 no.5
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• pp.453-472
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• 2013

This paper presents the structural behavior of CFRP (carbon fiber reinforced polymer) strengthened CFT (concrete-filled steel tubes) columns under axial loads. Circular and square specimens were selected to investigate the retrofitting effects of CFRP sheet on CFT columns. Test parameters are cross section of CFT, D/t (B/t) ratios, and the number of CFRP layers. The load and ductility capacities were evaluated for each specimen. Structural behavior comparisons of circular and rectangular section will be represented in the experimental result discussion section. Finally, ultimate load formula of CFRP strengthened CFT will be proposed to calculate the ultimate strength of CFRP strengthened circular CFT. The prediction values are in good agreement with the test results obtained in this study and in the literature.

• Journal of the Korean Applied Science and Technology
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• v.15 no.1
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• pp.63-78
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• 1998

A series of long chain N-acyl amino acid type anionic surfactants were prepared by treating fatty acid chlorides with three kinds of amino acids, that is, sodium N-acyl-sarcosinates, sodium N-acyl-N-methyl-${\beta}$-alaninates and sodium N-acyl-N-methyl-taurates in an alkaline solution. All prepared biodegradable surfactants were purified by thin layer chromatography and column chromatography, and identified their structures by spectral analysis.

• Journal of the Korean Applied Science and Technology
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• v.15 no.1
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• pp.99-108
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• 1998

Novel type surfactants containing two long-chain hydrophobic alkyl groups and two hydrophilic sulfonate groups were successfully synthesized by reactions of ethylene glycol diglycidyl ether with long-chain fatty alcohols, after then by reactions with 1,3-propane sultone and sodium hydride under THF solvent. All these compounds, so called Gemini surfactants, were purified by thin layer chromatography and column chromatography. Their structures were identified by FT-IR and $^{1}H$-NMR.

• Structural Engineering and Mechanics
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• v.60 no.6
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• pp.979-999
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• 2016

Stainless steel wire mesh (SSWM) is an alternative material for strengthening of structural elements similar to fiber reinforced polymer (FRP). Finite element (FE) method based Numerical investigation for evaluation of axial strength of SSWM strengthened plain cement concrete (PCC) and reinforced cement concrete (RCC) columns is presented in this paper. PCC columns of 200 mm diameter with height 400 mm, 800 mm and 1200 mm and RCC columns of diameter 200 mm with height of 1200 mm with different number of SSWM wraps are considered for study. The effect of concrete grade, height of column and number of wraps on axial strength is studied using finite element based software ABAQUS. The results of numerical simulation are compared with experimental study and design guidelines specified by ACI 440.2R-08 and CNR-DT 200/2004. As per numerical analysis, an increase in axial capacity of 15.69% to 153.95% and 52.39% to 109.06% is observed for PCC and RCC columns respectively with different number of SSWM wraps.

• Steel and Composite Structures
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• v.30 no.1
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• pp.69-79
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• 2019

Numerous problems have always vexed engineers with buckling, corrosion, bending, and over-loading in damaged steel structures. The present study aims to study the possible effects of Carbon Fiber Reinforced Polymer (CFRP) for strengthening deficient Steel Square Hollow Section (SHS) columns. To this end, the effects of axial loading, stiffness values, axial displacement, the shape of deficient on the length of steel SHS columns were evaluated based on a detailed parametric study. Ten specimens were tested to failure under axial compression in laboratory and simulated by using Finite Element (FE) analysis based on numerical approach. The results indicated that the application of CFRP sheets resulted in reducing stress in the damage location and preventing or retarding local deformation around the deficiency location appropriately. In addition, the retrofitting method could increase loading the carrying capacity of specimens.

• Structural Engineering and Mechanics
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• v.78 no.5
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• pp.545-556
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• 2021

The present experimental study evaluated the seismic performance of six engineered cementitious composite (ECC) columns strengthened with carbon fiber reinforced polymer (CFRP) laminates under cyclic lateral loading. The ECC columns damaged and crushed in the first stage of cyclic tests were repaired using the ECC with a certain polyvinyl alcohol (PVA) fiber and strengthened with flexural and sheer CFRP laminates and then re-assessed under the cyclic loading. The effects of some variables were examined on lateral displacement, energy absorption and dissipation, failure modes, crack patterns, load bearing capacity and plasticity, and the obtained results were compared with those of the first stage of cyclic tests. The results showed that retrofitting the ECC columns can improve their performance, plasticity and load-bearing threshold, delayed the concrete failure, changed the failure modes and increased the energy absorbed by the strengthened columns element by over 50%.