• 미생물학회지
• /
• 제54권1호
• /
• pp.18-23
• /
• 2018

히말라야산의 동토에서 발견된 Preussia sp.로부터 신규 벤조산 유도체 benzyl 2,4-di(benzyloxy)benzoate (1)와 6개의 알려진 화합물들(2-7)이 분리되었다. 신규물질 및 다른 화합물들의 구조는 1D-와 2D NMR실험 및 문헌값과의 비교에 의하여 결정되었다. 화합물 2-7은 동 속 및 Sporormiaceae과에서 처음으로 얻어졌다. 분리된 물질들은 쥐의 대식세포 RAW 264.7 cells에서 lipopolysaccharide (LPS) 처리에 의하여 유도된 nitric oxide (NO)의 생성량 억제를 측정하였다. 화합물 1번과 2번은 100 mg/ml의 농도로 처리했을 때 LPS에 의하여 유도된 NO 생성량을 각각 50.7% 및 88.5%까지 억제하는 것을 보였다.

• 한국과학교육학회지
• /
• 제43권3호
• /
• pp.209-223
• /
• 2023

이 연구는 초·중등 학생의 극지 소양 함양을 위해 개발된 극지 소양 교육 프로그램을 적용한 효과를 평가하고, 기후 변화 대응 교육을 위한 시사점을 도출하기 위해 수행되었다. Polar-ICE 단체가 정립한 7가지 극지 소양 원리를 중심으로 개발한 모듈식 프로그램 중 과학개념을 강조한 프로그램과 인문학과 사회학적 쟁점을 강조한 프로그램 등 두 과정에 적합한 세부 프로그램을 선정해서 구조화했다. 이들 두 가지 프로그램은 서울 소재 대학 과학교육과에서 주최한 토요 과학교실에서 초등학생과 중학생 26명에게 약 69시간에 걸쳐 적용되었다. 26명의 학생을 세 집단으로 나누어 두 집단에게는 각각 극지 소양을 위한 과학 교육 프로그램과 인문 사회학적 쟁점 교육 프로그램을 실시했고, 나머지 한 집단은 극지 소양과 관련 없는 일반 과학 교육을 적용하여 대조군이 되었다. 프로그램을 적용하기 전후에 세 집단의 학생들은 모두 동일하게 극지 소양 검사와 극지에 대한 연상 단어와 장면을 진술하는 설문에 응답했다. 검사 결과는 비모수검정 방법인 Wilcoxon의 대응쌍 부호순위로 나타내어 프로그램 적용 전후의 향상도를 비교했다. 연구 결과 인지적 측면에서 실험군과 대조군 모두 지식 측면에서 프로그램 적용 후 향상이 나타났으나 실험군이 대조군 보다 향상의 정도가 더 컸으며, 명시적으로 다룬 내용이나 소재에서 특히 분명한 차이를 나타냈다. 정의적 측면에서 프로그램 전후의 차이는 그다지 큰 차이를 나타내지 않았지만, 인문 사회학적 쟁점을 다루었던 집단은 통계적으로 유의미한 향상을 보였다. 극지 심상의 변화는 실험 집단인 두 집단이 대조 집단에 비해 단조로운 이미지에서 좀 더 다양한 이미지로 분산되는 경향이 보였다. 이상의 연구 결과를 바탕으로 극지 소양 원리 과학 교육 프로그램의 효과를 높이기 위해 유의할 점, 과학적 사고를 함양하고 지구계 교육에 도움이 되는 소재로 활용할 필요성, 극지에 대한 태도를 향상하기 위한 방안, 기후 위기 대응을 준비하는 학교 교육 과정과의 연계 등의 시사점을 제안했다.

• Parasites, Hosts and Diseases
• /
• 제32권1호
• /
• pp.57-60
• /
• 1994

Thelohanellus kitauei 포자의 운명을 조사하기 위하여 in vitro에서 극사탈출률을 경시적으로 조사하였다. 포자를 0.45% 및 0.9%식염수, 증류수 그리고 Tyrode액에 현탁시켜 $-70^{\circ}C$에 냉동하여 장기간 보존한 바 거의 모든 포자의 활성은 최초 냉동후 1.750일 째까지 초기와 거의 같았다. 0.45% 및 0.9%식염수 그리고 증류수에 현탁시켜 $5^{\circ}C$에 보존한 포자는 각각 1,628일, 1,614일 그리고 1,721일, 항생제를 첨가한 위의 현탁액에서는 각각 1,628일, 1.614일 그리고 1,714일에 모든 포자가 사멸하였다. 한편, Tyrode액에 현탁시킨 것은 모두 235일에 모든 포자가 사멸하였다.

• Ocean and Polar Research
• /
• 제28권2호
• /
• pp.107-117
• /
• 2006

Freshwater algal studies in North polar environments are relatively few. This study presented the algal-flora, -biomass and genetic features of dominant cells collected from temporary ponds around the Polar Research Station (PRS), Norway. Water samples were collected from 4 stations around PRS, and analyzed for their environmental and biological variables. Water temperature, salinity and conductivity ranged from 5 to $10^{\circ}C$, 0.1 to $0.3%_{\circ}$ and 0.21 to $0.36{\mu}S/cm$, respectively. Chlorophyll a concentration ranged from 1.8 to $11.1{\mu}g/l$, and that of the size-fractionated cells was recorded from 0.7 to $1.1{\mu}g/l$ in picoplankton 0.3 to $6.5{\mu}g/l$ in nanoplankton, and 0.4 to $3.9{\mu}g/l$ in microplankton respectively. Algal flora in the present study was recorded as 10 genera, in which Chlamydomonas, particularly, was dominant in all studied sites. By comparison of Chlamydomonas 18S rDNA sequences, including two isolates from PRS, they formed a distinct clade against others: sequence similarity was significantly low (<97.2%) with C. noctigama, being the highest score by BLAST search in GenBank. This study was valuable for basic knowledge regarding the freshwater algae around PRS and their genetic information.

• Asian Journal of Atmospheric Environment
• /
• 제12권3호
• /
• pp.274-288
• /
• 2018

Quantitative data of 50 non-polar organic compounds constituting $PM_{2.5}$ were continuously collected and analyzed from June 2016 to October 2017 (approximately 17 months) at Ichihara, one of the largest industrial areas in Japan. Target non-polar organic compounds including 21 species of polycyclic aromatic hydrocarbons (PAHs), 24 species of n-alkanes and 5 species of phthalate esters(PAEs) were simultaneously measured by gas chromatography/mass spectrometry. Basically, the average concentrations of the total PAHs, n-alkanes and PAEs in each season remained nearly level, and seasonal variations were little throughout the study period. These results suggest that the emission sources, which are not influenced by the seasons, are the dominant inputs for the target organic compounds. Diagnostic ratios of PAHs, assessment of n-alkane homologue distributions, carbon preference index, and the contribution of wax n-alkanes from plants were used to estimate source apportionments. These results indicate that anthropogenic sources were the main contributor for most PAHs and n-alkanes throughout the study period. The concentrations of PAEs selected in this study were low because emission amounts of these chemicals were little within the source areas of the sampling site. To our knowledge, this study is the first attempt to simultaneously measure a high number of non-polar organic compounds in $PM_{2.5}$ collected from the ambient air of Japan, and the resultant data will provide valuable data and information for environmental researchers.

• 수산해양기술연구
• /
• 제58권2호
• /
• pp.130-140
• /
• 2022

Recently, wideband acoustic technology has been introduced and started to be used in fisheries acoustic surveys in various waters worldwide. Wideband acoustic data provides high vertical resolution, high signal-to-noise ratio and continuous frequency characteristics over a wide frequency range for species identification. In this study, the main characteristics of wideband acoustic systems were elaborated, and a general methodology for wideband acoustic data analysis was presented using data collected in frequency modulation mode for the first time in Republic of Korea. In particular, this study described the data recording method using the mission planner of the wideband autonomous acoustic system, wideband acoustic data signal processing, calibration and the wideband frequency response graph. Since wideband acoustic systems are currently installed on many training and research vessels, it is expected that the results of this study can be used as basic knowledge for fisheries acoustic research using the state-of-the-art system.

• ALGAE
• /
• 제38권4호
• /
• pp.253-264
• /
• 2023

The Ceramiales is the most diverse and species-rich group (2,669 spp.) of red algae, and it is widely distributed from tropical to polar oceans. Mitochondrial genomes (mitogenomes) and other genes have contributed to our knowledge regarding the classification and phylogeny of this diverse red algal group; however, the mitogenome architecture remains understudied. Here, we compared 42 mitogenomes, including 19 newly generated in this study, to expand our knowledge. The number of genes in mitogenome varied from 43 to 68 due to gene duplication. The mitogenome architecture was also variable, categorized into four types (A-D): type A = ancestral type with a basic composition; type B = those with inverse transpositions; type C = those with inverted duplications; and type D = those with both inversion and duplication. The palindromic and inverted repeats were consistently found in flanking regions of the rearrangement, especially near the cob and nad6 genes. The three rearranged mitogenome architectures (types B, C, D) are the first report of these in red algae. Phylogenetic analyses of 23 protein-coding genes supported the current familial classification of the Ceramiales, implying that the diversity of mitogenome architecture preceded the phylogenetic relationships. Our study suggests that palindromic and inverted repeats may drive mitogenome architectural variation.

• Ocean and Polar Research
• /
• 제38권2호
• /
• pp.129-137
• /
• 2016

This study aimed to isolate and characterize sulfated polysaccharides (SPs) from Iridaea cordata and evaluate their anticancer activity. SPs of the Antarctic red seaweed were obtained by $CaCl_2$ (SP1) and ethanol precipitations (SP2) following diluted acid extraction at room temperature. Yields of SP1 and SP2 were approximately 14% and 23%, respectively, of the dry weight of red seaweed. The average molecular mass of the SP1 and SP2 was estimated about $1.84{\times}10^3$ and $1.42{\times}10^3kDa$, respectively, by size-fractionation High-Performance Liquid Chromatography (HPLC). From the High-Performance Anion-Exchange Chromatography-Pulsed Amperometric Detection (HPAEC-PAD) analysis, the main monosaccharide was galactose with glucose and fucose as minor components. The sulfate content of SP2 (40.4%) was slightly higher than that of SP1 (33.8%). The FT-IR spectra also showed characteristic band of carrageenan-like sulfated polysaccharides. Taken together the SPs are thought to be carrageenan-like sulfated galactan. The polysaccharides (SPs) from I. cordata exhibited weak antitumor activity against PC-3 (prostate cancer), HeLa (cervical cancer), and HT-29 (human colon adenocarcinoma). To our knowledge, this is the first data on biological activity of the Antarctic red seaweed I. cordata.

• 대한원격탐사학회지
• /
• 제21권1호
• /
• pp.91-98
• /
• 2005

On board satellite magnetometer measures all possible magnetic components, such as the core and crustal components from the inner Earth, and magnetospheric, ionospheric and' its coupled components from the outer Earth. Due to its dipole and non-dipole features, separation of the respective component from the measurements is most difficult unless the comprehensive knowledge of each field characteristics and the consequent modeling methods are solidly constructed. Especially, regional long wavelength magnetic signals of the crust are strongly masked by the main field and dynamic external field and hence difficult to isolate in the satellite measurements. In particular, the un-modeled effects of the strong auroral external fields and the complicated behavior of the core field near the geomagnetic poles conspire to greatly reduce the crustal magnetic signal-to-noise ratio in the polar region relative to the rest of the Earth. We can, however, use spectral correlation theory to filter the static lithospheric and core field components from the dynamic external field effects that are closely related to the geomagnetic storms affecting ionospheric current disturbances. To help isolate regional lithospheric anomalies from core field components, the correlations between CHAMP magnetic anomalies and the pseudo-magnetic effects inferred from satellite gravity-derived crustal thickness variations can also be exploited, Isolation of long wavelengths resulted from the respective source is the key to understand and improve the models of the external magnetic components as well as of the lower crustal structures. We expect to model the external field variations that might also be affected by a sudden upheaval like tsunami by using our algorithm after isolating any internal field components.

• Ocean and Polar Research
• /
• 제29권1호
• /
• pp.43-53
• /
• 2007

Recently, controversy over intellectual property rights for protecting genetic resources and traditional knowledge has been emerging. Very active debates and global discussions are being carried out in various international organizations for possible approaches to be taken for these properties, and for the fair and equal sharing of the benefits from these intellectual properties. There is a need to evaluate adopting a sui generis system which is being pushed by developing nations, or adopting a policy which will guaranteee benefit sharing such as sharing royalties from marketing final products, technical transfers, capacity building, and participating in research activities. Also, it is very important to examine the legal issues concerning genetic resources based on Convention on Biological Diversity for the fair and equal sharing of the benefits with developing nations, at the same time assuring developed nations of access to genetic resources.