• Korean Journal of Microbiology
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• v.52 no.2
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• pp.192-201
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• 2016

Recently, Psychrobacter sp. ArcL13 strain showing the extracellular lipase activity was isolated from the Chuckchi Sea of the Arctic Ocean. However, due to the low expression levels of the enzyme in the natural strain, the production of recombinant lipase is crucial for various applications. Identification of the gene for the enzyme is prerequisite for the production of the recombinant protein. Therefore, in the present study, a novel lipase gene (ArcL13-Lip) was isolated from Psychrobacter sp. ArcL13 strain by gene prospecting using PCR, and its complete nucleotide sequence was determined. Sequence analysis showed that ArcL13-Lip has high amino acid sequence similarity to lipases from bacteria of some Psychrobacter genus (84-90%) despite low nucleotide sequence similarity. The lipase gene was cloned into the bacterial expression plasmid and expressed in E. coli. SDS-PAGE analysis of the cells showed that ArcL13-Lip was expressed as inclusion bodies with a molecular mass of about 35 kDa. Refolding was achieved by diluting the unfolded protein into refolding buffers containing various additives, and the highest refolding efficiency was seen in the glucose-containing buffer. Refolded ArcL13-Lip showed high hydrolytic activity toward p-nitrophenyl caprylate and p-nitrophenyl decanoate among different p-nitrophenyl esters. Recombinant ArcL13-Lip displayed maximal activity at $40^{\circ}C$ and pH 8.0 with p-nitrophenyl caprylate as a substrate. Activity assays performed at various temperatures showed that ArcL13-Lip is a cold-active lipase with about 40% and 73% of enzymatic activity at $10^{\circ}C$ and $20^{\circ}C$, respectively, compared to its maximal activity at $40^{\circ}C$.

• Journal of the Korean Home Economics Association
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• v.37 no.5
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• pp.61-84
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• 1999

The purpose of this study is to understand the context of cosumers' lives in Korea with a narrative analysis method. The epistemological orientation of eh narrative analysis is Interpretivism, which blends the two polar philosophical perspectives, Empiricism and Rationalism, and includes Narrotology, Hermeneneutics, Semiotics, and Structural Criticism. Narrative analysis takes as its object of investigation the story itself. This study collects eleven narrative plots from four housewives, into which Labov's structural approach is applied. This study shows clearly that the socio-cultural environment in which consumers live has strong influence on their consumption behavior and also reveals that narrativization tells not only about past actions but how individuals understand those actions, that is, meaning.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.2
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• pp.129-142
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• 1986

Fifty-five local collections of buck wheat, Fagopyrum esculentum, were investigated their ratios of long-styled (LS) and short-styled (SS) flowers, fertility, meiosis of megaspore and microspore mother cell, female and male gametogenesis, and egg apparatus in accordance with the sowing seasons (spring, summer), altitudes (20m, 50-100m, 300m), and parent style types (L, S). Also they were embryologically investigated the fertility, fertilizing phenomenon and proembryogenesis by the legitimate and illegitimate pollination. There were no differences in the ratios of long-styled and short-5tyled flowers along with altitudes, but more irregularness was observed in plain area than that in the mountaineous or coastal area. LS versus SS ratios by sowing seasons were significantly separated into 1 : 1 in the summer sowing (P 0.1), but they were irregularly separated in the spring sowing. The segregating ratios by parent style types showed more number of short-styled flower in the spring sowing, and were statistically seperated into 1 : 1 in the summer sowing (P 0.25), regardless to parent style types. In the artificial legitimate union, the seed setting rates of the summer sowing (59-61%) were much higher than those of the spring sowing (about 30%), but in the artificial illegitimate union the seed setting rates were only fructified about 0.8-1.8% in the spring sowing. The seed setting rates in accordance with flowering stages were larger in turn early, middle, late, in the summer sowing. The grain number and grain weight per plant of short-styled flower were more than those of long-styled one regardless to style types. The 1,000 grain weight of long-styled flower was heavier than that of short-styled one in large grain, but it was lighter than that of short-styled flower in small or medium grain. The percentage of normal female and male gametogenesis in the summer sowing were higher than those in the spring sowing. The ovule was atropous and two polar nuclei were a synkarion before flowering. The pollens germinated at 30 minuts after pollination and the pollen tube grew continually and penetrated into micropyle at 1.5-2 hours and the two male nuclei fertilized with egg nucleus at 3 -5 hours after pollination. Flertilizing times in summer were shorter than in autumn. The fertilized egg was divided in a small apical cell toward the interior of the embryo sac and a large basal cell toward the micropyle cell at 15-24 hours after pollination, and division times in summer were shorter than in autumn. The proembryo began the embryogenesis at 7-8 days and formed itself into the perfect embryo at 15 days after pollination.

• The Korean Journal of Pesticide Science
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• v.7 no.3
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• pp.176-181
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• 2003

The leaching behaviour of [aniline-$^{14}C$]mefenacet in soil was investigated using glass columns (5 cm I.D. $\times$ 30 cm. H) packed with two types of soils with different physicochemical properties. $^{14}C$-Mefenacet (8.33 kBq) and mefenacet (in total, 1.05 mg/kg) were treated onto soil columns and rice plants (Oryza sativa L.) were grown for 17 weeks on these columns. Leachates from the columns were collected at the rate of 122.5 mL per week. $^{14}C$-Activities leached from soil A (OM, 3.1%; CEC, 86 mmol(+)/kg; texture, loam) columns with and without rice plants were 1.95 and 4.19% of the originally applied, whereas those from soil B (OM, 1.3%; CEC, 71 mmol(+)/kg; texture, loam) were 2.69 and 7.05%, respectively. These results indicated that larger amounts of $^{14}C$ were percolated from soil B with less organic matter and from the columns without vegetation. $^{14}C$-Activities absorbed by rice plants from soil A and B were 8.95 and 8.47%, respectively, most of which remained in the root and shoot excluding unhulled grains and ears without grains. $62\sim73%$ of the originally applied $^{14}C$ remained in the depth of $0\sim5cm$ in soil. The mass balance indicated that the losses by volatilization and/or mineralization amounted to $3.4\sim9.2%$ of the originally applied. $^{14}C$-Radioactivities in the aqueous phase of the leachates ranged from 59.4 to 97.7% of the radioactivities in leachates, showing the fast transformation of mefenacet to the polar metabolites.

• 한국해양학회지
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• v.27 no.3
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• pp.250-258
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• 1992

The oxygen isotope composition of surface waters in the Bransfield Strait was determined as one extra state variable in order to characterize water masses in the region, since salinity is significantly modified due to the freezing and ice-melting in the polar region. The salinity, temperature, and $\delta$/SUP 18/O values vary from 34.0 to 34.5$\textperthousand$, -.05 to 2.1$^{\circ}C$ and -0.50 t -0.26$\textperthousand$, respectively. The combined effects of evaporation, precipitation, freezing, ice-melting are reflected in the widely scattered data. Although it is small, the distribution of $\delta$/SUP 18/O of the Bransfield Strait is strongly affected by the freezing-ice melting rather than the evaporation-precipitation. The ice melted fresh water which has higher temperature, depleted salinity and nutrients may be injected to the Bransfield Strait from the north. The concentrations of nutrients are decreasing gradually from the north to the south. The waters were characterized by two groups of higher (about 19.4) and lower N/P ratio (about 16.7). The lower N/P ratio is found in the northern part where ice-melted fresh water is injected. and the higher N/P ratio is found in the southern part of the Bransfield Strait. Although more precise work is needed, the deference of N/P ratio can be an evidence of the ice melted water injection to the Bransfield Strait. Chlorophyll a concentrations, in general, increase from northwest (Waddell Sea) to the southeast (Smith and Hosseason Islands). Probably the injection of nutrient depleted fresh water from the ice melting reduce the chlorophyll a concentration.

• Korean Journal of Food Science and Technology
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• v.30 no.4
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• pp.781-786
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• 1998

To assess effect of surface area on the oxidative stability in thermooxidative system, soybean oil and lard with different surface area $(0.04{\;}cm^2/g,{\;}0.08{\;}cm^2/g,{\;}0.12{\;}cm^2/g{\;}and{\;}0.16{\;}cm^2/g)$ were heated 8 hrs/day at $185^{\circ}C$. The iodine values of soybean oil heated for 64 hrs with surface area of $0.12{\;}cm^2/g$ and $0.16{\;}cm^2/g$ were 113.1 and 116.9, while those of the oil heated for the same length time with surface area of $0.04{\;}cm^2/g$ and $0.08{\;}cm^2/g$ were 126.4 and 125.9, respectively. The same trend was observed in lard, but less markedly than in soybean oil. The polar lipid content, dielectric constant and refractive index of soybean oil and lard heated with surface area above $0.12{\;}cm^2/g$ significantly increased as the heating time increased, while those of the oil heated with surface area below $0.08{\;}cm^2/g$ slowly increased as the heating time increased. On the other hand, the conjugated diene content of soybean oil heated with surface area above $0.12{\;}cm^2/g$ oil increased as the heating time increased while ratio of linoleic acid to palmitic acid decreased as the heating time increased. However, the conjugated diene content and ratio of linoleic acid to palmitic acid of lard were not significantly different depending on the surface area. The results obtained from this investigation indicated that the thermooxidative stability of oil heated with surface area below $0.08\;}cm^2/g$ was better than that of oil heated with surface area above $0.12{\;}cm^2/g$ (P<0.05).

• Journal of the Korean Applied Science and Technology
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• v.34 no.4
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• pp.827-838
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• 2017

Twenty male soccer players volunteered as the experimental subjects. The subjects were randomly assigned to the "Winter training with Cornus officinalis extract intake group"(WCI; n=10) and "Winter training group"(WT; n=10). The variables of blood fatigue substance, muscle damage and liver function where measured in all the subjects before the start of the Cornus officinalis intake after at the end of the 4 weeks winter training. Winter training program was proceeded with 6 days a week for 4 weeks. Exercise intensity was measured using the HRR(70~95%). Changes in the heart rate were also measured with the Polar. WCI group were ingested 80 mL of Cornus officinalis extract after every breakfast and dinner for 4 weeks. The test data were analyzed by paired t-test, and independent t-test(%diff), and the alpha level of p<.05 was set for all tests of significance. Lactate in WCI group had significantly decreased, and the changes between the groups were significantly lower in the WCI group than in the WT group. Ammonia in WCI group had significantly increased. CK in WCI group had significantly decreased. LDH in both group had significantly decreased. AST in WCI group had significantly decreased, and the changes between the groups were significantly lower in the WCI group than in the WT group. ALT in WCI group had significantly decreased. In conclusion, the results of this study show that Cornus officinalis extract seem to have a positive influence on blood fatigue substance, muscle damage and liver function. Therefore Cornus officinalis extract is considered to be able to contribute an ergogenic aids.

• Korean Journal of Remote Sensing
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• v.23 no.6
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• pp.507-520
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• 2007

Satellite passive microwave(PM) sensors have been observing polar sea ice concentration(SIC), ice temperature, and snow depth since 1970s. Among them SIC is playing an important role in the various studies as it is considered the first factor for the monitoring of global climate and environment changes. Verification and correction of PM SIC is essential for this purpose. In this study, we calculated SIC from KOMPSAT-1 EOC images obtained from Arctic sea ice edges from July to August 2005 and compared with SSM/I SIC calculated from NASA Team(NT) algorithm. When we have no consideration of sea ice types, EOC and SSM/I NT SIC showed low correlation coefficient of 0.574. This is because there are differences in spatial resolution and observing time between two sensors, and the temporal and spatial variation of sea ice was high in summer Arctic ice edge. For the verification of SSM/I NT SIC according to sea ice types, we divided sea ice into land-fast ice, pack ice, and drift ice from EOC images, and compared them with SSM/I NT SIC corresponding to each ice type. The concentration of land-fast ice between EOC and SSM/I SIC were calculated very similarly to each other with the mean difference of 0.38%. This is because the temporal and spatial variation of land-fast ice is small, and the snow condition on the ice surface is relatively dry. In case of pack ice, there were lots of ice ridge and new ice that are known to be underestimated by NT algorithm. SSM/I NT SIC were lower than EOC SIC by 19.63% in average. In drift ice, SSM/I NT SIC showed 20.17% higher than EOC SIC in average. The sea ice with high concentration could be included inside the wide IFOV of SSM/I because the drift ice was located near the edge of pack ice. It is also suggested that SSM/I NT SIC overestimated the drift ice covered by wet snow.

• Korean Journal of Environmental Agriculture
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• v.40 no.4
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• pp.353-358
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• 2021

BACKGROUND: Prochloraz has been widely used as an imidazole fungicide on fruits and vegetables in Korea. Analytical approaches to evaluate prochloraz residues in herbal medicine are required for their safety management. In this study, we developed a GC-ECD method for quantitative determination of prochloraz in Platycodi Radix. The metabolite 2,4,6-trichlorophenol (2,4,6-T) was used as a target compound to evaluate total prochloraz residues as it is categorized to a representative residue definition of prochloraz. All residues containing 2,4,6-T were converted to 2,4,6-T and subjected to GC-ECD. METHODS AND RESULTS: In order to verify the applicability, the method was optimized for determining prochloraz and it metabolite 2,4,6-T in Platycodi Radix. Prochloraz and its metabolite 2,4,6-T residuals were extracted using acetone. The extract was diluted with and partitioned directly into dichloromethane to remove polar co-extractives in the aqueous phase. The extract was decomposed to 2,4,6-T, and then the partitioned ion-associate was finally purified by optimized aminopropyl solid-phase extraction (SPE). The limits of quantitation of the method (MLOQs) were 0.04 mg/kg and 0.02 mg/kg, respectively for prochloraz and 2,4,6-T, considering the maximum residue level (MRL) of prochloraz as 0.05 mg/kg in Platycodi Radix. Recovery tests were carried out at two levels of concentration (MLOQ, 10 MLOQ) and resulted in good recoveries (82.1-89.7%). Good reproducibilities were obtained (coefficient of variation < 2.8%), and the linearities of calibration curves were reasonable (r² > 0.9986) in the range of 0.005-0.5 ㎍/mL. CONCLUSION(S): The method developed in this study was successfully validated to meet the guidelines required for quantitative determination of pesticides in herbal medicine. Thus, the method could be useful to monitor prochloraz institutionally in herbal medicine.

• Korean Journal of Remote Sensing
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• v.37 no.5_1
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• pp.1177-1186
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• 2021

As the Arctic melt ponds play an important role in determining the interannual variation of the sea ice extent and changes in the Arctic environment, it is crucial to monitor the Arctic melt ponds with high accuracy. Ice, Cloud, and Land Elevation Satellite-2 (ICESat-2), which is the NASA's latest altimeter satellite based on the green laser (532 nm), observes the global surface elevation. When compared to the CryoSat-2 altimetry satellite whose along-track resolution is 250 m, ICESat-2 is highly expected to provide much more detailed information about Arctic melt ponds thanks to its high along-track resolution of 70 cm. The basic products of ICESat-2 are the surface height and the number of reflected photons. To aggregate the neighboring information of a specific ICESat-2 photon, the segments of photons with 10 m length were used. The standard deviation of the height and the total number of photons were calculated for each segment. As the melt ponds have the smoother surface than the sea ice, the lower variation of the height over melt ponds can make the melt ponds distinguished from the sea ice. When the melt ponds were extracted, the number of photons per segment was used to classify the melt ponds covered with open-water and specular ice. As photons are much more absorbed in the water-covered melt pondsthan the melt ponds with the specular ice, the number of photons persegment can distinguish the water- and ice-covered ponds. As a result, the suggested melt pond detection method was able to classify the sea ice, water-covered melt ponds, and ice-covered melt ponds. A qualitative analysis was conducted using the Sentinel-2 optical imagery. The suggested method successfully classified the water- and ice-covered ponds which were difficult to distinguish with Sentinel-2 optical images. Lastly, the pros and cons of the melt pond detection using satellite altimetry and optical images were discussed.