• Clinical and Experimental Pediatrics
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• 제48권5호
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• pp.500-505
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• 2005

Purpose : This study was designed to estimate the prevalence of Mycoplasma pneumoniae infection during an epidemic period by means of examining the antibody conversion rate and to investigate the association of the antibody conversion with age, initial antibody titer, and atopy. Methods : We chose 191 children whose antibody titer to M. pneumoniae was negative, 1 : 40, or 1 : 80 during the first half of 2003. After the second half of 2003 when the M. pneumoniae epidemic occurred, follow-up collection of sera was performed during the first half of 2004. M. pneumoniae antibody titer was measured by Serodia-Myco II particle agglutination test. Results : Of 191 children, antibody conversion was detected in 83 children(43.5 percent). No significant difference was found between the conversion and non-conversion group with respect to age, sex and atopy. Dividing the subjects into four groups by age, results on the antibody conversion rate revealed no significant differences between the groups. Assessed by initial antibody titer, a diminished trend of conversion rate was observed in children with 1 : 80 titer but the difference was not significant. There was no significant difference in the antibody conversion rate between atopic and non-atopic children. Conclusion : Based on the antibody conversion rate in this study, the prevalence of M. pneumoniae infection during an epidemic period was estimated to be 43.5 percent. This high infection rate suggests that during an epidemic, we should bear in mind M. pneumoniae as an important etiologic agent for respiratory infection in children.

• Annals of Clinical Neurophysiology
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• 제13권1호
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• pp.54-57
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• 2011

The Guillain-Barre Sydrome (GBS) is post-infectious autoimmune disease and it could be caused by auto-antibodies produced after infections. Mycoplasma pneumoniae is one of rare cause of GBS and known to be associated with antibody to galactocerebroside (GalC) which is a major neutral glycolipid constituent of myelin. We report a case of GBS with immunoglobulin M GalC antibody after M. pneumoniae infection.

• Journal of environmental and Sanitary engineering
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• 제15권1호
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• pp.34-38
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• 2000

In the screening of phenol-degrading bacteria, a strain showing good growth in media containing phenol was isolated by using enrichment culture from various sample and identified as genus Klebsiella pneumoniae. The optimal temperature and pH for cell growth of Klebsiella pneumoniae was $35^{\circ}C$ and 8.0, respectively. When phenol was added to the minimal media as a sole source of carbon and energy, the concentration of maximum and optimum for cell growth was 1,200ppm and 1,000ppm, respectively. It was observed that Klebsiella pneumoniae was able to degrade 98% of phenol (1,000ppm) after 40hr in culture. The isolated could utilize various kinds of aromatic compounds and showed good growth in presence of phenol, m-cresol and 3-methyl catechol.

• Korean Journal of Food Science and Technology
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• 제24권1호
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• pp.29-30
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• 1992

Three microorganisms were isolated from staled market soybean curd and confirmed their reproducibility of putrefaction. The isolates were identified as Acinetobacter calcoaceticus var. anitrat(97.9%) and Klebsiella pneumoniae subgroup pneumoniae(99.0%). The third microorganism has same characteristics as A. calcoaceticus var. anitrat except mucoid production.

• Childhood Kidney Diseases
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• 제3권1호
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• pp.104-108
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• 1999

Atypical hemolytic uremic syndrome associated with neuraminidase-producing Streptococcus pneumoniae usually associated with invasive infection such as fulminant pneumonia, sepsis, and meningitis and may occur earlier in lift and has a higher mortality rate than typical hemolytic uremic syndrome. We have experienced a 22-month-old female patient with hemolytic uremic syndrome associated with S. pneumoniae pneumonia and empyema. The patient was treated with ceftriaxone and washed red blood cell transfusion. As the disese course could be aggravated by the use of blood products containing anti-Tomsen-Friedenreich antigen, early recognition and sensible use of blood products such as washed RBC might lead to the improved outcome.

• Korean Journal of Veterinary Research
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• 제60권3호
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• pp.183-186
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• 2020

This paper describes the development of neurological signs of two prematurely born calves four days after birth. The pathological examination results indicated fibrinopurulent polyserositis, including meningoencephalitis with suppurative bronchopneumonia. Bovine viral diarrhea virus subtype 2a was detected in most of the internal organs, and the bacterial colonies cultured from the samples were identified as Klebsiella (K.) pneumoniae. Molecular analysis via multilocus sequence typing identified a different K. pneumoniae isolate in each calf-type 14 in calf A and type 65 in calf B. This is the first report identifying K. pneumoniae sequence types 14 and 65 in cattle.

• Journal of Veterinary Clinics
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• 제36권5호
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• pp.245-247
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• 2019

Taylorella equigenitalis (T. equigenitalis), Klebsiella pneumoniae (K. pneumoniae), and Pseudomonas aeruginosa (P. aeruginosa) are sexually transmittable bacteria known to cause venereal diseases (VD) in horses. T. equigenitalis causes contagious equine metritis (CEM), which is a considerable concern for equine breeding industry. K. pneumoniae and P. aeruginosa may cause endometritis and infertility in susceptible mares. The purpose of this study was to investigate the prevalence of these bacteria among breeding Thoroughbreds in South Korea. External genital swabs were collected from 178 breeding Thoroughbreds, including 11 stallions and 167 mares. The samples were tested using a commercial multiplex real-time PCR kit. T. equigenitalis, P. aeruginosa, and K. pneumoniae were present in 5.6%, 7.3%, and 5.6% of tested Thoroughbreds, respectively. The results highlight the need for regular testing of South Korean Thoroughbreds, particularly those used for breeding, for these bacteria. The regular pre-breeding test for these bacteria will prevent health complications for the horse and financial losses for the owner as a result of VD.

• Proceedings of the PSK Conference
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.70.2-71
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• 2003

Quinolone resistance in Streptococcus pneumoniae is related to mutations in the DNA gyrase and topoisomerase IV genes. DW-286a displayed potent activity against S. pneumoniae C9211 (MIC, 0.015 ${\mu}$g/ml) compared with gemifloxacin (MIC, 0.06 ${\mu}$g/ml). This study was performed to analyze the ability of DW-286a to cause resistance development in S. pneumoniae and to establish whether DNA gyrase or topoisomerase IV is primary target. DW-286a resistant mutants of S. pneumoniae C9211 were generated by stepwise selection at increasing drug concentration. (omitted)

• Genomics & Informatics
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• 제20권4호
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• pp.47.1-47.13
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• 2022

Klebsiella pneumoniae is a gram-negative bacterium that is known for causing infection in nosocomial settings. As reported by the World Health Organization, carbapenem-resistant Enterobacteriaceae, a category that includes K. pneumoniae, are classified as an urgent threat, and the greatest concern is that these bacterial pathogens may acquire genetic traits that make them resistant towards antibiotics. The last class of antibiotics, carbapenems, are not able to combat these bacterial pathogens, allowing them to clonally expand antibiotic-resistant strains. Most antibiotics target essential pathways of bacterial cells; however, these targets are no longer susceptible to antibiotics. Hence, in our study, we focused on a hypothetical protein in K. pneumoniae that contains a DNA methylation protein domain, suggesting a new potential site as a drug target. DNA methylation regulates the attenuation of bacterial virulence. We integrated computational-aided drug design by using a bioinformatics approach to perform subtractive genomics, virtual screening, and fingerprint similarity search. We identified a new potential drug, koenimbine, which could be a novel antibiotic.

• Journal of the Korea Society of Computer and Information
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• 제21권5호
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• pp.135-139
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• 2016

Scrophularia ningpoensis hemsl has been traditionally used in China and Vietnam for treatment of bacteria, atopy, pimple, tonsillitis, angina and encephalitis for a long time. The main objectives of this study were to evaluate the antibacterial activity of the Scrophularia ningpoensis hemsl extract on biofilm formation of Klebsiella pneumoniae. Antibacterial activity was conducted using disc diffusion assay and minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) were determined using the broth micro dilution method in accordance to Clinical and Laboratory Standards Institute guidelines(CLSI). Furthermore, cytotoxicity on L929 were assessed using animal cell culture for the proliferation test(MTT cell assay) and the biofilm forming capacity of the K. pneumoniae were determined using the colony forming unit (CFU) assay. The extract exhibited considerable antibacterial activity. K. pneumoniae was susceptible to the extract with the MIC and MBC of 0.1875 and $1.5mg/m{\ell}$ respectively. Cytoxicity test in L929 showed no sign of toxicity at the concentration of $0.75mg/m{\ell}$ and at the same concentration the extract caused inhibition of bacterial biofilm formation. The extract of Scrophularia ningpoensis hemsl possesses an in vitro antibacterial antibiofilm activities against K. pneumoniae, with no sign of cytoxicity on L929.