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Chitin의 효소적 가수분해 특성에 대한 속도론적 연구 (Kinetics on the Specificity of Enzymatic Hydrolysis of Chitin)

  • 이은영;김광
    • KSBB Journal
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    • 제13권1호
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    • pp.44-51
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    • 1998
  • Hydrolysis and adsorption reversibility experiments were run for initial enzyme activity of 4.48, 9.65, 11.19 and 17.14U/mL at a temperature 30$^\circ C$. The chitin particle size corresponded to a mean particle diameter of 0.127mm, and the initial concentration of chitin was 10mg/mL. After approximately 2hrs, the enzyme activity remained constant in a speudo-steady state. The amounts in the bulk [E] and the amounts of enzyme adsorbed on the chitin surface [E] are plotted on Lineweaver-Burk plot to yield a linear relationship with a correlation coefficient of 0.99, a slope of 2.79cm$^-1$ and an intercept of 0.08$\textrm{cm}^2$/U. From this parameters, the values of [E$_T$] and $K_E$ were calculated to be 12.5U/cm$^2$ and 34.88U/mL. respectively, Adsorption isotherm of the enzyme on the particles showed a well developed plateau of 1.35$\times$10$^-3$, 4.72$\times$10$^-3$, 4.42$\times$10$^-3$, 8.58$\times$10$^-3$U/cm$^2$ at 30$^\circ C$. To determine the specificity of chitinase for crystalline chitin, the free energy of adsorption was measured, and its was determined as about -14.62~-18.8kJ/mol.

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간호사간 직장 내 괴롭힘 관련변인에 대한 체계적 문헌고찰과 메타분석 (The Related Factors to Workplace Bullying in Nursing: A Systematic Review and Meta-analysis)

  • 강지연;이민주
    • 성인간호학회지
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    • 제28권4호
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    • pp.399-414
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    • 2016
  • Purpose: The purpose of this study was to review and identify factors relevant to workplace bullying in nursing. Methods: Twenty-three studies that met the criteria were selected from a sample of twenty-six studies. These articles were retrieved from a central literature databases (N=13,241). The total correlational effect size (ESr) for each related factor was calculated from Fisher's Zr. A funnel plot inspection (similar to scatter plot) with a trim-and-fill method was used to assess the publication bias of the meta-analyzed studies. Results: From the systematic review, fifty-one factors were identified as having an influencing effect. Fourteen factors (five individual and nine organizational factors) were eligible for meta-analysis. The individual factors included, self-esteem (ESr=-.31), psychological capital (ESr=-.26), and marital status (ESr=-.06) which were significantly correlated with workplace bullying. Organizational factors included, organizational tolerance (ESr=.48), supervisor incivility (ESr=.47), job stress (ESr=.46), group morale (ESr=-.36), group support (ESr=-.35), supervisor leadership (ESr=-.35), group identity (ESr=-.33), and structural empowerment (ESr=-.27). These factors were significantly correlated with workplace bullying. There were no publication biases except for both individual and organizational factors. Conclusion: Organizational factors have more of an greater impact than individual factors on workplace bullying. The results of this study support the need for intervention at the organizational level.

자생 수목 그루터기를 이용한 자연식생복원 녹화공법 연구(I) (A Study on Revegetation Measures with Recycling Root-stock of Native Tree(I))

  • 오구균;권태호;배중남;박석곤
    • 한국환경복원기술학회지
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    • 제6권5호
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    • pp.28-39
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    • 2003
  • This study was carried out to elucidate effective restoration measures for natural forest with recycling native tree un site from November 2001 to October 2002 to obtain a basic information for revegetation measure, eight experimental treatment was done and the length of stump, root-ball size of stump, antisepsis treatment of trunk cut, Planting season and contents of organic matter in soil were effective on regrowth of root-stock. Thirteen tree species including Quercus acutissima among twenty tree species showed outstanding sprout and survival rate(over 90 percent), Planting in November and combinated planting with 5 trees and 9 shrubs of root-stock per 100$m^2$ plot showed a good growth. And 10 percent of organic matter plot showed a good crown coverage.

Effects of hippotherapy on children with cerebral palsy: systematic review and meta-analysis

  • Kim, Kwon-Hoi;Lee, Suk-Min
    • Physical Therapy Rehabilitation Science
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    • 제9권1호
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    • pp.55-65
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    • 2020
  • Objective: The most important goal of hippotherapy is to improve function, gait, and balance. The purpose of this study was to investigate the effects of hippotherapy on function, gait, and balance of children with cerebral palsy (CP). Design: A systematic review and meta-analysis. Methods: We've searched the PubMed, EMBASE, and Cochrane Library CENTRAL databases for English experimental studies published up until September 2018. To analyze the effects of hippotherapy on the function, gait, and balance of children with cerebral palsy, the systematic review and meta-analysis were performed on Patient/Participants/Population Problem, Intervention, Comparison, Outcome with Timing, Setting Study Design. Papers retrieved from the database were removed from the literature management database. Methodological quality evaluation was evaluated using Cochrane's risk of bias. Data was analyzed using the Revman 5.3 program of the Cochrane library. Results: In this study, we found that the effect size of hippotherapy, denoted as d, was -3.82, and that hippotherapy had the most effect on gait, but no statistical significant difference was observed. We also found no significant difference in function and balance after hippotherapy. It was found from the funnel plot that there was no publication bias as the plot was symmetrically distributed around the dotted line. Conclusions: There are many unclear studies and a lack of randomized controlled trials (RCTs) in this research area. More RCTs on the effects of hippotherapy on children with CP should be conducted in the future.

생태적 특성을 고려한 도시환경림 조성기법연구( I ) -서울시 개포 근린공원을 중심으로- (A Study on the Plannig Methods of Urban Environmental Forest for Considering Ecological Characteristics( I )-In the case of Gaepo neighborhood Park in the Seoul City-)

  • 이경재
    • 한국조경학회지
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    • 제23권3호
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    • pp.48-58
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    • 1995
  • This study was conducted to investigate the plant community and to propose ecological planning methods for urban environmental forest on Gaepo neighborhood park, Gangnamgu in Seoul. Thirty-six plots that the size of each plot was 10$\times$10m(100\ulcorner) were set up and the vegetation analysis was carried out. Field survey was executed Apirl, 1994. The actual vegetation was consisted of Poplulus$\times$albaglandulosa community(73.03%), Robinia pseudoacacia community(8.30%) P. $\times$ albaglandulosa-R.pseudoacacia community(1.81%) and environmental forest (15.22%) in the survey site. According to mean importance values of each plot, survey area were divided into ten forest communities. As for the analysis of plant community structure which was classified into ten forest communities, the importance values, ecological diversity, number of species, number of individuals, basal area and crown coverage of urban environmental forest were relatively lower than those of the native community. In urban environmental forest of Gaepo neighborhood park, it showed problems for the planting method. So urban environmental forest should be constructed considering ecological characteristics based on above results.

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An Optimal Model Prediction for Fruits Diseases with Weather Conditions

  • Ragu, Vasanth;Lee, Myeongbae;Sivamani, Saraswathi;Cho, Yongyun;Park, Jangwoo;Cho, Kyungryong;Cho, Sungeon;Hong, Kijeong;Oh, Soo Lyul;Shin, Changsun
    • 스마트미디어저널
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    • 제8권1호
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    • pp.82-91
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    • 2019
  • This study provides the analysis and prediction of fruits diseases related to weather conditions (temperature, wind speed, solar power, rainfall and humidity) using Linear Model and Poisson Regression. The main goal of the research is to control the method of fruits diseases and also to prevent diseases using less agricultural pesticides. So, it is needed to predict the fruits diseases with weather data. Initially, fruit data is used to detect the fruit diseases. If diseases are found, we move to the next process and verify the condition of the fruits including their size. We identify the growth of fruit and evidence of diseases with Linear Model. Then, Poisson Regression used in this study to fit the model of fruits diseases with weather conditions as an input provides the predicted diseases as an output. Finally, the residuals plot, Q-Q plot and other plots help to validate the fitness of Linear Model and provide correlation between the actual and the predicted diseases as a result of the conducted experiment in this study.

Analyzing performance of time series classification using STFT and time series imaging algorithms

  • Sung-Kyu Hong;Sang-Chul Kim
    • 한국컴퓨터정보학회논문지
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    • 제28권4호
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    • pp.1-11
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    • 2023
  • 본 논문은 순환 신경망 대신 합성곱 신경망을 사용하여 시계열 데이터 분류 성능을 분석한다. TSC(Time Series Community)에는 GAF(Gramian Angular Field), MTF(Markov Transition Field), RP(Recurrence Plot)와 같은 전통적인 시계열 데이터 이미지화 알고리즘들이 있다. 실험은 이미지화 알고리즘들에 필요한 하이퍼 파라미터들을 조정하면서 합성곱 신경망의 성능을 평가하는 방식으로 진행된다. UCR 아카이브의 GunPoint 데이터셋을 기준으로 성능을 평가했을 때, 본 논문에서 제안하는 STFT(Short Time Fourier Transform) 알고리즘이 최적화된 하이퍼 파라미터를 찾은 경우, 기존의 알고리즘들 대비 정확도가 높고, 동적으로 feature map 이미지의 크기도 조절가능하다는 장점이 있다. GAF 또한 98~99%의 높은 정확도를 보이지만, feature map 이미지의 크기를 동적으로 조절할 수 없어 크다는 단점이 존재한다.

잉크의 중첩인쇄순서에 기인하는 색차의 광학적 해석에 관한 연구 (A Study on Optical Analysis of the Color Difference Caused by the Overprinting Sequence of Ink)

  • 강상훈
    • 한국인쇄학회지
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    • 제16권1호
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    • pp.39-53
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    • 1998
  • The extraction rate of ginger from Korean-grown ginger root with supercritical carbon dioxide was measured as a function of flow rate of supercritical carbon dioxide, particle size, temperature and pressure. the extraction rate increased as the particle size decreased due to a decrease in the diffusion path. The extraction rate were independent of flow rate of supercritical carbon dioxide in a plot of ginger oil yield versus extraction time. This indicated that the extraction process is controlled by intraparticle diffusion within a particle of ginger root. In the case of temperature and pressure effect, the experimental results showed that the extraction rate decreased with an increase in temperature and increased with an increase in pressure.

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비혼탁도 법을 이용한 나노 콜로이드 입자의 크기 결정 (Determination of Sizes of Nano-Particles by Specific Turbidimetry)

  • 임경희
    • 한국응용과학기술학회지
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    • 제25권1호
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    • pp.107-114
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    • 2008
  • In this article a reliable and rapid method based on specific turbidimetry is proposed for the determination of sizes of nanoparticles. Conventionally in specific turbidimetry specific turbidities for a colloidal dispersion are measured as a function of light wavelength, and compared to theoretical values calculated from Mie scattering theory for presumed particle sizes. In contrast specific turbidity at a fixed wavelength is measured in the proposed method, and particle sizes are determined from the prepared calibration curve. The calibration curve is a plot of specific turbidity vs particle size and in this case the specific turbidities are measured for a couple of samples of known sizes.

Evaluation of spatial pressure distribution during ice-structure interaction using pressure indicating film

  • Kim, Hyunwook;Ulan-Kvitberg, Christopher;Daley, Claude
    • International Journal of Naval Architecture and Ocean Engineering
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    • 제6권3호
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    • pp.578-597
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    • 2014
  • Understanding of 'spatial' pressure distribution is required to determine design loads on local structures, such as plating and framing. However, obtaining a practical 'spatial' pressure distribution is a hard task due to the sensitivity of the data acquisition frequency and resolution. High-resolution Pessure-Idicating Flm (PIF) was applied to obtain pressure distribution and pressure magnitude using stepped crushing method. Different types of PIF were stacked at each test to creating a pressure distribution plot at specific time steps. Two different concepts of plotting 'spatial' pressure-area curve was introduced and evaluated. Diverse unit pixel size was chosen to investigate the effect of the resolution in data analysis. Activated area was not significantly affected by unit pixel size; however, total force was highly sensitive.