• 한국균학회지
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• 제24권2호통권77호
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• pp.89-92
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• 1996

폐면을 이용한 느타리버섯(Pleurotus ostreatus (Fr.) Quel)의 가을재배에서 배지량에 따른 자실체 수량과 초발이 소요일수에 미치는 영향을 시험한 결과 전 배지 공히 배지량이 증가할수록 수량이 증가되었으며, 그 중 폐면 + 볏짚 (6 : 4, V/V) 혼합처리가 증수특이 가장 컸으며$(10{\sim}22%)$, 폐면단용처리가 입상량간 증수특이 가장 완만하였다$(7{\sim}15%)$. 초발이 소요일수는 입상량이 적을수록 빨랐고, 특히 왕겨혼합처리(5 : 5, V/V) $50\;kg/m^2$가 다소 빠른 경향이었다.

• Preventive Nutrition and Food Science
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• 제12권4호
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• pp.222-228
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• 2007

This study was conducted to investigate the minimization of off-flavor occurrence and the maintenance of high quality in modified atmosphere packaged Pleurotus ostreatus during the storage. There are 4 treatments used to preserve high quality and for deodorization of MAP mushroom: Artemisia princeps, Artemisia capillaries, green tea and activated charcoal. The mushrooms were packed in polyethylene film with each treatment and were stored at 5 and $20^{circ}C$. No difference was observed in weight loss, $CO_2\;and\;O_2$ concentration, or color of mushrooms packed with or without treatment. However, the principal component analysis (PCA), electronic nose, revealed differences in off-flavor occurrence between control (MAP mushroom without treatment) and treatment groups at $5^{\circ}C$. This result suggested that Artemisia princeps and Artemisia capillaries was masking the off-flavor in MAP mushroom because the unique flavor of them was strongly revealed and green tea and activated charcoal might have a role of removing the off-flavor by adsorbing ethanol and acetaldehyde, which is known to cause off-flavor. The sensory test showed that Artemisia princeps and Artemisia capillaries dough treatment inhibited microbial growth.

• 한국식품영양과학회지
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• 제29권1호
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• pp.85-92
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• 2000

This study was carried out to storage of Pleurotus ostreatus packed in various trays made by different matrials-expanded polystyrene (EPS), rice straw pulp(RP), and RP trays coated with starch, carboxyl methyl cellulose (CMC), polyacrylamide (PAM), alkylketen dimer (AKD), rosin size (RS) and polyethylene glycol (PEG) at 8~1$0^{\circ}C$ and 70~80% relative hymidity for 6 days. At 6 days storage, weight increase of RP and CMC tray was lower (p<0.05) than those of others and the weight of oyster mushroom packed in RP, CMC and AKD tray decreased lower(p<0.05) than those of others. Carbon dioxide contents in trays were increased from 0.5% to 5.5%~8.6% within 1 day storage. Oxygen contents in trays were sharply decreased from 20% to 0.8%~8.2% within 1 day storage. Lightness of oyster mushroom increased all trays. Off flavor appeared stronly in oyster mushroom packed in EPS, AKD and PEG during storage, but was not detected in mushroom packed in starch and CMC until 4 days storage.

• The Plant Pathology Journal
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• 제21권4호
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• pp.343-348
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• 2005

The partial nucleotide sequences of the genomic dsRNA mycoviruses infecting Pleurotus ostreatus (isolates ASI2596, ASI2597, and Bupyungbokhoe) and Agaricus blazei Murrill were determined and compared with those of the other dsRNA mycoviruses. Partial nucleotide sequences of the purified dsRNA from ASI2596 and ASI2597 revealed RNA-dependent RNA polymerase sequences that are closely related to Oyster mushroom isometric virus 2, while nucleotide sequences and the deduced amino acid sequence from dsRNA mycovirus infecting Agaricus blazei did not show any significant homology to the other dsRNA mycoviruses. Specific primers were designed for RT-PCR detection of these dsRNA viruses and were found to specifically detect each dsRNA virus. Northern blot analysis confirmed the homogeneity of RT-PCR products to each purified dsRNA. Altogether, our results suggest that these virus-specific primer sets can be employed for the specific detection of each dsRNA mycovirus in infected mushrooms.

• Journal of Microbiology
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• 제41권1호
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• pp.52-57
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• 2003

The mutational spectra in the manganese (II) peroxidase gene (mnp) of the Pleurotus ostreatus mutants induced by gamma radiation (Co$\^$60/) give evidence to prove the effect of gamma radiation on the gene. mnp of each mutant was cloned, sequenced and analyzed. Among the 1941 base pairs of the sequenced region of the mnP genes of 4 mutants (PO-5,-6,-15 and -16), nine mutational hotspots on which the same base was mutated simultaneously were found, additionally 6 mutations were also found at different positions in the mnp gene. These mutation-spectra were predominantly A:T\longrightarrowG:C transitions (50.1%). By the analysis of putative amino acid sequences, PO-5 and PO-16 mutants have 3 and 1 mutated residues, respectively. Since the mutational spectra reported herein are specific to the mnp gene, we propose that the mutational hotspots for the gamma radiation could be in the gene(5) within cells.

• 한국버섯학회지
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• 제17권2호
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• pp.41-46
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• 2019

This study was carried out to investigate optimum wind velocity for growth of Pleurotus ostreatus. In the chamber experiments, the divergence of pileus was $63^{\circ}$ in 0.3m/s, and it showed the tendency that the more the air velocity was increased, the more the divergence became small. And the ratio of commercial yields was 96% in the air velocity of 0.3m/s. In the cultivation room experiments, the divergence of pileus was $64^{\circ}$ in 0.3m/s, and it was the tendency that the more the air velocity was fast, the more the divergence became larger. And the commercial yields was similar to the trend of the chamber experiment as 94.8% in 0.3m/s, and the quality characteristics of the fruit-body showed the uniform quality in comparison with other treatment because the standard deviation of the size of the pileus, etc. was the lowest in 0.3m/s like the chamber experiment.

• 한국버섯학회지
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• 제2권1호
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• pp.42-43
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• 2004

Labor required for managing and harvesting the oyster mushroom bed was evaluated. Although vinyl mulching cultivation method needs more hours for spawning, it saves more than 50% of labor for harvesting and managing of the mushroom bed. Harvesting hour of 1st-3rd flush in vinyl mulching method was 48~50% for Plerutus ostreatus and 36~41% for P. sajor-caju. Labor for bed management after harvesting in vinyl mulching method was also 38~50% for P. ostreatus compared to conventional method, and 20~35% for P. sajor-caju. Vinyl mulching is believed to be a very efficient method for saving labor in oyster mushroom cultivation.

• 생명과학회지
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• 제6권3호
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• pp.193-197
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• 1996

The action spectrum for mycelial growth and fruitbody primordium formation in Pleurotus ostreatus has been studied by irradiation at various wavelengths. Effective wavelengths. Effective wavelengths were distributed from near ultraviolet to blue region of spectrum. The most effect of light was observed in the region between 340 to 500 nanometers. The light intensity required to obtain of the maximum effect at the most effective wavelengths(430-500nm), was over 6.8 mW/Cm$^{2}$. Up to 6.8 mW/Cm$^{2}$, the primordium formed about 25 hr after the start of illumination. The higher the light intensity, the earlier the formation of the primordium formation : Up to about 6.8 mW/Cm$^{2}$.

• Journal of Species Research
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• 제3권2호
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• pp.123-126
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• 2014

We present a list of the beetle species associated with the oyster mushroom, Pleurotus ostreatus Fr. in the birch forest of Onon-Balj National Park in Mongolia. A total of 21 species in seven families were found in this fungus, including fungivorous species: Litargus japonicus Reitter and Mycetophagus antennatus (Reitter) (Mycetophagidae), Pseudamblyopus similis (Lewis) (Erotylidae), and Lordithon sp. (Staphylinidae) etc. Both adult and larval forms are presented in the checklist along with the type of fungal association, of which approximately 52 percent are obligate mycetobionts. The main trends of the ecological and trophic specialization of the mycetophilic beetles are discussed.

• 미생물학회지
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• 제25권2호
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• pp.148-156
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• 1987

Extracellular laccase (E.C. 1.10.3.2) from the culture filtrate of Pleurotus ostreatus was purified by ammonium sulfate precipctation, protamine sulfate precipitation, DEAE-Sephadex A-50 ion exchange chromatography and Sephadex G-100 gel permeation chromatography. The molecular weight of the enzyme was estimated by SDS-polyacrylamide gel electrophoresis to be 58,000 and the isoelectric point was 3.75. The optimum temperature for the enzyme was about $45^{\circ}C$ and the optimum pH was 6.5. The enzyme was found to be stable at temperature below $35^{\circ}C$ and rapidly inactivated at higher temperatures. Km values for ferulic acid, vanillic acid, dihydroxyphenylalanine (DOPA) were 48.6.$\mu$M, 0.52mM, and 2.73mM, respectively, which indicates that the enzyme has much higher affinity towards ferulic acid. The reaction products of the enzyme were separated by TLC and HPLC.