• 한국콘텐츠학회논문지
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• 제20권9호
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• pp.169-186
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• 2020

이 연구는 지역 지상파 방송사의 유튜브 시사뉴스 콘텐츠의 특성과 이용자 반응 간의 상관관계를 살펴보고 대응 방안을 탐색하는 데 목적이 있다. 유튜브 오픈 API를 활용하여 전국 31곳의 지역 지상파 방송사의 유튜브 뉴스 채널에서 한 달 간 업로드 된 3,950건의 동영상을 표본으로 추출하였다. 개별 동영상을 분석 단위로 설정하고 형식적 특성과 내용적 특성, 이용자 반응 등을 내용분석 하였다. 연구결과, 디지털 전용 콘텐츠를 생산하고 있었지만 매우 낮은 비율이었으며, 대부분은 제목과 형식이 그대로 업로드 되는 방송 다시보기 영상이었다. 또한, 상당수의 채널이 TV편성과 같은 종합 채널의 형태로 운영되고 있어 독자적인 플랫폼으로서의 전문성을 보여주지 못하고 있었다. 이는 지역 방송사의 유튜브 채널은 TV나 자사 홈페이지와의 차별성이 부재하며, 여전히 TV플랫폼의 보조적 역할이나 아카이브 기능에 편향되어 있음을 알 수 있는 부분이다. 그럼에도 불구하고, 지역 전문성과 유튜브 감성을 반영한 소수의 디지털 전용 콘텐츠는 높은 조회 수와 반응을 이끌며 온라인 공간에서의 확장성을 보여주었고 지역 방송사 유튜브 뉴스 채널에 현실적이고 효과적인 방향성을 제시했다.

• 대한지리학회지
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• 제51권2호
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• pp.235-253
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• 2016

중국은 2004년부터 자동차 공업 관련 신산업 정책을 추진하기 시작하였다. 이와 함께 기존의 맹목적인 외자유치에서 높은 기술수준을 지닌 외자기업을 유치하는 방향으로 선회하였다. 이러한 정책적 환경 변화는 수많은 다국적 기업들 및 협력기업들의 중국 진출을 본격화시켰으며, 한국의 완성차 및 부품기업 또한 진출이 이루어졌다. 외자기업의 착근과정에서 나타난 제도적 변화에 의한 혁신적인 기술 적용 필요성은 기업의 기술 혁신 필요성과 조응하였는데, 효율적 공간 전략이 요구되었다. 첫째, 제도적 변화에 따른 로컬 파트너십 활용과 부품기업 동반진출 전략, 둘째, 기술혁신을 통한 모듈화 및 공용 플랫폼 전략, 셋째, 지방정부 인센티브 정책 활용 전략이 그것이다. 특히 생산 네트워크 내 행위자들은 직서열 공급방식을 통한 부품 공급망을 따라 전후방 연계의 흐름이 나타났으며 관계성이 강화되었다. 따라서 부품기업들은 원활한 협력 체제를 위해 전방기업 및 모듈 단위 중심으로 집적하였다. 그 결과 북쪽의 베이징 현대를 중심으로 하는 북쪽 지역, 남쪽의 동풍열달기아를 중심으로 하는 남쪽 지역, 공용 부품을 공급하기 위한 칭다오를 중심으로 하는 중부 지역, 일반 공용 부품 및 원자재를 공급하는 상하이를 중심으로 하는 기타 지역으로 생산 공간의 분화가 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제30권5호
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• pp.743-748
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• 2017

Objective: Based on rapid advancement of genetic modification techniques, genomic editing is expected to become the most efficient tool for improvement of economic traits in livestock as well as poultry. In this study, we examined and verified the nickase of mutated CRISPR-associated protein 9 (Cas9) to modulate the specific target gene in chicken DF1 cells. Methods: Chicken myostatin which inhibits muscle cell growth and differentiation during myogenesis was targeted to be deleted and mutated by the Cas9-D10A nickase. After co-transfection of the nickase expression vector with green fluorescent gene (GFP) gene and targeted multiplex guide RNAs (gRNAs), the GFP-positive cells were sorted out by fluorescence-activated cell sorting procedure. Results: Through the genotyping analysis of the knockout cells, the mutant induction efficiency was 100% in the targeted site. Number of the deleted nucleotides ranged from 2 to 39 nucleotide deletion. There was no phenotypic difference between regular cells and knockout cells. However, myostatin protein was not apparently detected in the knockout cells by Western blotting. Additionally, six off-target sites were predicted and analyzed but any non-specific mutation in the off-target sites was not observed. Conclusion: The knockout technical platform with the nickase and multiplex gRNAs can be efficiently and stablely applied to functional genomics study in poultry and finally adapted to generate the knockout poultry for agribio industry.

• Genomics & Informatics
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• 제13권4호
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• pp.137-145
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• 2015

Selective sweep can cause genetic differentiation across populations, which allows for the identification of possible causative regions/genes underlying important traits. The pig has experienced a long history of allele frequency changes through artificial selection in the domestication process. We obtained an average of 329,482,871 sequence reads for 24 pigs from three pig breeds: Yorkshire (n = 5), Landrace (n = 13), and Duroc (n = 6). An average read depth of 11.7 was obtained using whole-genome resequencing on an Illumina HiSeq2000 platform. In this study, cross-population extended haplotype homozygosity and cross-population composite likelihood ratio tests were implemented to detect genes experiencing positive selection for the genome-wide resequencing data generated from three commercial pig breeds. In our results, 26, 7, and 14 genes from Yorkshire, Landrace, and Duroc, respectively were detected by two kinds of statistical tests. Significant evidence for positive selection was identified on genes ST6GALNAC2 and EPHX1 in Yorkshire, PARK2 in Landrace, and BMP6, SLA-DQA1, and PRKG1 in Duroc. These genes are reportedly relevant to lactation, reproduction, meat quality, and growth traits. To understand how these single nucleotide polymorphisms (SNPs) related positive selection affect protein function, we analyzed the effect of non-synonymous SNPs. Three SNPs (rs324509622, rs80931851, and rs80937718) in the SLA-DQA1 gene were significant in the enrichment tests, indicating strong evidence for positive selection in Duroc. Our analyses identified genes under positive selection for lactation, reproduction, and meat-quality and growth traits in Yorkshire, Landrace, and Duroc, respectively.

• 한국수자원학회논문집
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• 제46권4호
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• pp.389-400
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• 2013

이 논문에서는 관망시스템의 마찰항을 보정하기 위해서, 부정류 마찰 모형과 Levenberg Marquardt 방법을 합성하였다. 부정류 마찰항을 고려하기 위한 방법으로 빈도 의존 마찰항을 사용하였으며, 특성선 방법을 모형 개발의 기반으로 하였다. 최적화에 필요한 Hessian과 Jacobian 행렬을 구하기위해서 수압을 직접 마찰항에 미분한 항을 계산하였으며, 특성선 방법상에서의 다양한 수압과 유량에 대한 마찰계수의 민감도를 수식으로 유도하였다. 간단한 관망을 가정한 뒤, 갑작스런 밸브의 거동으로 도입된수압의 시계열을 확보하였고, 이를 이용하여 정상류 마찰 모형과 부정류 마찰모형의 마찰항 보정을 수행하였다. 제안된 방법과 진화 연산 알고리즘의 마찰항 수렴거동을 비교하였으며, Leveberg Marquardt 방법의 안정적이고 신속한 수렴결과를 확인하였다.

• 사상체질의학회지
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• 제30권3호
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• pp.74-94
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• 2018

Introduction The case report provides pivotal basis for clinical research and education, however systematically organized guideline for reporting case report was not properly applied and it tackled the development of traditional medicine in Korea. The purpose of this study was to implement guideline for reporting CAse REport(CARE guideline) incorporating unique characteristics of Sasang medicine with the help of examples and explanations. Methods and Results The guideline for reporting CAse REport for Sasang medicine(CARES guideline) including diagnosis of traditional medicine and treatment with acupuncture and medical herbs was suggested, and practical examples explaining details of each CARES items were provided. Discussion and Conclusion This study would provide foundation for consensus-based and common CARE guideline for traditional Korean medicine. And, the CARES guideline would provide clinical platform for developing refined Sasang type differentiation technique using reported biopsychosocial and clinical type-specific features and realistic cases for Problem Based Learning courses as evidence-based education.

• 한국융합학회논문지
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• 제12권9호
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• pp.31-37
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• 2021

최근들어 가전시장에서 융합기술을 이용한 제품 차별화는 소비자로부터 많은 호응을 얻고 있다. 그러나 에어컨 제품은 기계적 운영에서 센서와 플랫폼이 결합된 인공지능을 위한 융합기술이 적용하는 초기단계에 있어서 많은 연구개발이 필요한 분야이다. 본 논문에서는 IR-UWB 기술을 이용한 비접촉 방식의 생체신호(호흡수, 이동) 수집기술을 개발하였다. 생체신호를 이용하여 사용자의 위치에 따라 에어컨 방향을 제어한다. 또한 최적의 수면 환경을 제공하기 위해 수면상태를 모니터링 한다. 감성조명과 ASMR은 안락하고 감성적인 삶의 공간을 제공하기 위해 개발되었다. 그리고 개발된 융합기술을 기반으로 편리하고 안락한 휴식공간을 제공하는 지능형 스마트 에어컨 서비스 플랫폼을 제안하였다.

• Biomolecules & Therapeutics
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• 제27권3호
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• pp.302-310
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• 2019

Melanoma cells have been shown to respond to BRAF inhibitors; however, intrinsic and acquired resistance limits their clinical application. In this study, we performed RNA-Seq analysis with BRAF inhibitor-sensitive (A375P) and -resistant (A375P/Mdr with acquired resistance and SK-MEL-2 with intrinsic resistance) melanoma cell lines, to reveal the genes and pathways potentially involved in intrinsic and acquired resistance to BRAF inhibitors. A total of 546 differentially expressed genes (DEGs), including 239 up-regulated and 307 down-regulated genes, were identified in both intrinsic and acquired resistant cells. Gene ontology (GO) analysis revealed that the top 10 biological processes associated with these genes included angiogenesis, immune response, cell adhesion, antigen processing and presentation, extracellular matrix organization, osteoblast differentiation, collagen catabolic process, viral entry into host cell, cell migration, and positive regulation of protein kinase B signaling. In addition, using the PAN-THER GO classification system, we showed that the highest enriched GOs targeted by the 546 DEGs were responses to cellular processes (ontology: biological process), binding (ontology: molecular function), and cell subcellular localization (ontology: cellular component). Ingenuity pathway analysis (IPA) network analysis showed a network that was common to two BRAF inhibitorresistant cells. Taken together, the present study may provide a useful platform to further reveal biological processes associated with BRAF inhibitor resistance, and present areas for therapeutic tool development to overcome BRAF inhibitor resistance.

• Journal of Plant Biotechnology
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• 제46권3호
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• pp.217-227
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• 2019

Production of therapeutic monoclonal antibodies (mAbs) using a plant platform has been considered an alternative to the mammalian cell-based production system. A plant-derived mAb CO17-1AK ($mAb^P$ COK) can specifically bind to various types of cancer cell lines. The target protein of $mAb^P$ COK is the epithelial cell adhesion molecule (EpCAM) highly expressed in human epithelial cancer cells, including breast and colorectal cancer cells. It has been hypothesized that its overexpression supports tumor growth and metastasis. A ganglioside is extended well beyond the surfaces of the various cell membranes and has roles in cell growth, inflammation, differentiation, and carcinogenesis. However, the regulation of EpCAM gene expression in breast cancers and the role of gangliosides in oncogenesis are unclear. Here, the purpose of this study was to determine the effects of $mAb^P$ COK on human breast cancer cell proliferation, apoptosis, and ganglioside expression patterns. Our results show that treatment with $mAb^P$ COK suppressed the growth of breast cancer cells and induced apoptotic cell death. It also upregulated the expression of metastasis-related gangliosides in breast cancer cells. Thus, treatment with $mAb^P$ COK may have chemo-preventive therapeutic effects against human breast cancer.

• 생체재료학회지
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• 제22권4호
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• pp.279-289
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• 2018

Background: Cell behavior is the key to tissue regeneration. Given the fact that most of the cells used in tissue engineering are anchorage-dependent, their behavior including adhesion, growth, migration, matrix synthesis, and differentiation is related to the design of the scaffolds. Thus, characterization of the scaffolds is highly required. Micro-computed tomography (micro-CT) provides a powerful platform to analyze, visualize, and explore any portion of interest in the scaffold in a 3D fashion without cutting or destroying it with the benefit of almost no sample preparation need. Main body: This review highlights the relationship between the scaffold microstructure and cell behavior, and provides the basics of the micro-CT method. In this work, we also analyzed the original papers that were published in 2016 through a systematic search to address the need for specific improvements in the methods section of the papers including the amount of provided information from the obtained results. Conclusion: Micro-CT offers a unique microstructural analysis of biomaterials, notwithstanding the associated challenges and limitations. Future studies that will include micro-CT characterization of scaffolds should report the important details of the method, and the derived quantitative and qualitative information can be maximized.