• Korean Journal of Medicinal Crop Science
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• v.1 no.1
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• pp.38-42
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• 1993

The present study was carried out to assess the possibility of rapid multiplication of Zingiber mioga ROSC. through in vitro culture of shoot-apex. The factor investigated was effect of various growth regulators on shoot-apex culture. The shoot-apex cultured of M. S. (Murashige and Skoog) medium developed into plantlet in 12 Weeks. M. S. medium containing NAA at 05ppm and BA 5.0ppm was found to be optimal for growth of in vitro plantlet.

• Korean Journal of Plant Tissue Culture
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• v.26 no.1
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• pp.37-40
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• 1999

This study was carried out to determine the optimal conditions for the production of plants derived from the unpollinated ovary culture of Chinese chive (Allium tuberosum Rottl.). The Chinese chive collected from Korea showed much higher frequency of plantlet formation than those from Japan in the culture of unpollinated ovaries. Among the collections, 'Youngiljaerae' showed the highest frequency of plantlet formation. The MS basal medium was superior to B/sub 5/ in plantlet formation. The ovaries inoculated on the 2,4-D-free medium were directly induced plantlets without callus formation. Floral parts inoculated as a unit played important roles in callus formation and plant production. The frequency of callus and plantlet formation was higher in the culture of ovary with anthers than that of ovary alone.

• Journal of Plant Biotechnology
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• v.7 no.2
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• pp.129-134
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• 2005

Callus induction from a leaf explant has been achieved in Lilium Oriental hybrid 'Casa Blanca'. The highest frequency of callus induction was obtained on MS medium supplemented with 0.5 mg/L BA and 2.0 mg/L NAA after 2 months of culture. The cultures maintained continuously without change in color and type of callus when they cultured in the dark. Plantlet regeneration with a high frequency was achieved from induced calli on the same medium. A number of shoots are formed from one cluster of callus, and bulblets developed into intact plantlets after transfer to hormone-free MS medium. No phenotypic variations were observed among regenerants. Enhancement in plantlet regeneration via callus formation would be expected to facilitate the efficiency of transformation of this Oriental hybrid 'Casa Blanca'.

• Plant Biotechnology Reports
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• v.4 no.3
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• pp.229-235
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• 2010

In this study, we established an in vitro regeneration system to maximize the recovery of leafy perilla (Perilla frutescens L. Britton) plantlets as part of developing a molecular biotechnology-based metabolic engineering program for this crop plant. Hypocotyl segments including the apical buds were used as explants for the direct production of shoots without an interim callus phase. The number of shoots produced from the apical buds peaked within 3-4 weeks, and the shoots were subsequently cultured on Murashige and Skoog (MS) media supplemented with 2 mg $1^{-1}$ benzylaminopurine (BA). Spontaneous rhizogenesis was observed after 7-10 days of culture on MS media without hormonal additives. The rooted shoots developed into normal plants in soil after hardening on distilled water for 3-4 days. The average plantlet regeneration frequency was higher for the apical buds (64.33%) than for the top (15.66%), middle (4%), and basal (1.33%) segments of the hypocotyls. This regeneration system demonstrates a capacity for high-frequency plantlet recovery and thus should be considered for use in the genetic manipulation of leafy perilla.

• Journal of Life Science
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• v.6 no.1
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• pp.40-47
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• 1996

Experiments were conducted to determine the effects of plant growth regulators on in vitro flowering and micropropagation of Gentiana scabra Bunge which had been used the cut flower, pot flower ornamental and medicinal plants. Flower bud formation was affected by GA$_{3}$ and kinetin. The optimum concentrations for flower bud formation was observed at 0.5 mg/l kinetin and GA$_{3}$ , while kinetin was favorable. More flowerings result from the interaction of GA$_{3}$ and kinetin at in a combination of 0.1 mg/l kinetin + o.1 mg/l GA$_{3}$, but the optimum concentration of GA$_{3}$ and kinetin was decreased. All concentrations of kinetin with 0.1 mg/l GA$_{3}$ or O mg/l GA$_{3}$ + 0.5 mg/l kientin reduced t (weeks needed for 50% plantlets). The plantlet growth was affected by GA$_{3}$ and kinetin during plantlet culture. More lateral shots and better shoot length per plantlet were obtained as GA$_{3}$ and kinetin concentration were increased up to 1.0 mg/l. The number of per plantlet was greater increased in MS medium containing GA$_{3}$ than kinetin. Interaction was exhibited at lower concentration with 0.5mg/l GA$_{3}$ and kinetin, but not in higher concentration with 1.0 mg/l GA$_{3}$ and kinetin. Higher pod diameter increased seed germination, while lower pod diameter was obtained from abnormal plantlet. MA medium containing 0.5 mg/l GA$_{3}$ significantly increased germination without regard to pod diameter.

• Journal of Bio-Environment Control
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• v.20 no.4
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• pp.283-289
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• 2011

This study was conducted to examine the effects of defoliation treatment on the growth and yield of strawberry ($Fragaria{\times}ananassa$ cv. Maehyang and Seolhyang) during nursery period. Leaves of strawberry plantlet had been removed except two, three and four fully expanded leaves until planting date. As the intensity of defoliation was strong, the petiole length was reduced and overgrowth of strawberry plantlet was suppressed. Outer diameter of crown in defoliation treatments significantly decreased but inner diameter of crown was not significant. Number of primary roots of the 3 leaves or 4 leaves defoliation treatment generally tended to increase, but there was not significantly different among treatments. Fresh weight and leaf area in the defoliation treatments significantly decreased and the root weight were higher in partial 3 leaves or 4 leaves defoliation treatment but was not significantly different among treatments. Because T/R ratio decreased significantly as growth inhibition of above-ground part compared to underground part, it is considered easy to take rooting after plantlet plating. As the intensity of defoliation was strong, chlorophyll contents tended to decrease significantly. Reduction of the endogenous nitrogen by defoliation effectively led to promote floral differentiation at low temperature and short day condition. This promoted timing of budding and flowering and also induced uniform flowering after plantlet planting. Marketable fruit yield of 3 leaves defoliation treatment tended to be higher than the control.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.6
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• pp.438-441
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• 2003

The present study was carried out to assess the possibility of rapid multiplication of Curcuma longa Linne through in vitro culture of shoot-apex. The factor investigated was effect of various growth regulators on shoot-apex culture. The shoot-apex cultured of MS(Murashige and Skoog) medium developed into plantlet in 16 Weeks. M.S. medium containing NAA at 0.5 ppm and BA 5.0 ppm was found to be optimal for growth of in vitro plantlet

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.145-145
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• 2005

• Korean Journal of Plant Tissue Culture
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• v.22 no.5
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• pp.273-276
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• 1995

In vitro propagation of Neoregeria carorinae 'Tricolor' was achieved by using immature flowers and lateral buds, and the plantlets from tissue culture were transplanted and cultivated in greenhouse. The picking times of explants to decrease disappearance of stripes, and in vivo the growth and flowering of regenerated plantlets as influenced by in vivo healed nun were investigated. The normal plantlet were obtained at a frequency of 67%, in the culture of immature flowers picked at 4 weeks after flower bud differentiation, while all leaf stripes disappeared in the culture of immature flowers picked 1 and 5 weeks after flower bud differentiation. In vivo growth of plantlet from immature flower buds was better than those from lateral buds, and the flowering of 27.8% showed in the greenhouse culture of plantlet from immature culture, but the plantlets from lateral buds did not flower at all. The plantlets rooted on the medium with 0.5 mg/L IBA were the most favorable in green house culture, and the kinds and concentrations of auxin in vitro did not have any influence on variation of plane cultured in greenhouse.

• Journal of Plant Biotechnology
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• v.42 no.2
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• pp.111-116
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• 2015

Interest and great demand for blueberry (Vaccinium corymbosum) have increased, as V. corymbosum is now one of the most economically important crops in Korea. It is expected that blueberry production and the area planted for cultivation will increase consistently in the years ahead because of high profitability and the consumer's demand for healthy ingredients. Effective mass production of blueberry is urgently needed for commercial cultivation establishment, but a main limitation is lack of a propagation system that produces a disease-free plant material for commercial plantation. A large amount of research has focused entirely on developing tissue culture techniques for blueberry propagation. However, controlling fungal and bacterial contamination of woody plant material is extremely difficult. Our study was conducted to investigate the effect of biocide addition during the in vitro culture of blueberry on plantlet growth and contamination occurrence. Four biocides, including Plant Preservative Mixture ($PPM^{TM}$), vancomycin, nystatin and penicillin G, were used in varying concentrations during the in vitro propagation of blueberry. When nystatin was added into the medium at low concentrations, the overall growth of blueberry plantlets was retarded. Addition of vancomycin and penicillin G in high concentrations decreased contamination but induced plantlet mortality. On the other hand, when 1ml/L $PPM^{TM}$ was added, the growth characteristics of blueberry plantlets did not significantly differ from non-treatment (control), and the contamination occurrence rate was very low. From these results, we found that the addition of the appropriate biocide could provide an effective method to reduce contamination in the culture process, thereby raising in vitro production efficiency.