• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.140-140
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• 2017

Nutritious and functional foods from crop have received great attention in recent years. Colored-grain wheat contains high phenolic compound and a large number of flavonoid. The anthocyanin and polyphenolic synthesis and accumulation is generally stimulated in response to biotic or abiotic stresses. Here, we analyzed genome wide transcripts in seedling of colored-grain wheat response to ABA and PEG treatment. About 900 and 1500 transcripts (p-value < 0.05) from ABA and PEG treatment were aligned to IWGSC1+popseq DB which is composed of over 110,000 transcripts including 100,934 coding genes. NR protein sequences of Poaceae from NCBI and protein sequence of transcription factors originated from 83 species in plant transcription factor database v3.0 were used for annotation of putative transcripts. Gene ontology analysis were conducted and KEGG mapping was performed to show expression pattern of biosynthesis genes related in flavonoid, isoflavonoid, flavons and anthocyanin biopathway. DroughtDB (http://pgsb.helmholtz-muenchen.de/droughtdb/) was used for detection of DEGs to explain that physiological and molecular drought avoidance by drought tolerance mechanisms. Drought response pathway, such as ABA signaling, water and ion channels, detoxification signaling, enzymes of osmolyte biosynthesis, phospholipid metabolism, signal transduction, and transcription factors related DEGs were selected to explain response mechanism under water deficit condition. Anthocyanin, phenol compound, and DPPH radical scavenging activity were measured and antioxidant activity enzyme assays were conducted to show biochemical adaptation under water deficit condition. Several MYB and bHLH transcription factors were up-regulated in both ABA and PEG treated condition, which means highly expressed MYB and bHLH transcription factors enhanced the expression of genes related in the biosynthesis pathways of flavonoids, such as anthocyanin and dihydroflavonols in colored wheat seedlings. Subsequently, the accumulation of total anthocyanin and phenol contents were observed in colored wheat seedlings, and antioxidant capacity was promoted by upregulation of genes involved in maintaining redox state and activation of antioxidant scavengers, such as CAT, APX, POD, and SOD in colored wheat seedlings under water deficit condition. This work may provide valuable and basic information for further investigation of the molecular responses of colored-grain wheat to water deficit stress and for further gene-based studies.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.104-104
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• 2017

Sorghum (Sorghum bicolor (L.) Moench.) is one of the most important crops for human and animal nutrition. Nonetheless, sorghum has a cyanogenic glucoside compound which can be degraded into hydrogen cyanide, toxic to humans and animals even with tiny amount. In consequence, breeding materials with a low cyanide level has been a top priority in sorghum breeding programs. To fulfill our long-term goal, we are screening sorghum accessions with low cyanide level, which would be an important breeding material for food safety. We collected seeds of various sorghum accessions and analyzed relevant metabolites to find useful breeding materials of sorghum accessions containing low cyanide. Fourteen wild relatives were obtained from the University of Georgia in US, a reference accession BTx623, and three local varieties from National Agrobiodiversity Center of Rural Development Administration in Korea, and one wild species from the Wild Plant Resources Seed Bank of Korea University in Korea. Sorghum plants were grown in plastic greenhouse under natural conditions. After growing, leaf samples were harvested at different developmental stages: seedling phase, vegetative phase (right before flowering), and reproductive phase (ripening). Using collected samples, quantification analysis were performed by an HPLC system for three metabolites (dhurrin, 4-hydroxybenzaldehyde, and 4-hydroxyphenylacetic acid) in sorghum plants. Prior to metabolome analysis, specific experimental condition for HPLC system was set to be able to separate three metabolites simultaneously. Under this condition, these metabolites were quantified in each accession by HPLC system. We observed that the metabolite contents were changed differently by developmental stages and accessions. We clustered these results into five groups as patterns of their contents by developmental stages. Most of accessions showed that 4-hydroxybenzaldehyde content was very high at seedling stage and decreased rapidly at vegetative phase. Interestingly, the patterns of dhurrin content were very different among clusters. However, 4-hydroxyphenylacetic acid content was maintained at low levels by developmental stages in most accessions. The results would demonstrate how dhurrin and alternative degradation pathways are differentiated in each accession.

• Korean Journal of Food Science and Technology
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• v.52 no.1
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• pp.40-45
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• 2020

Over 500 genetically modified organisms (GMOs) have been developed since 1996, of which nearly 44% have glufosinate herbicide-tolerant traits. Identification of specific markers that can be used to identify herbicide-tolerant traits is challenging as the DNA sequences of the gene(s) of a trait are highly variable depending on the origin of the gene(s), plant species, and developers. To develop specific PCR marker(s) for the detection of the glufosinate-tolerance trait, DNA sequences of several pat or bar genes were compared and a diverse combination of PCR primer sets were examined using certified reference materials or transgenic plants. Based on both the qualitative and quantitative PCR tests, a primer set specific for pat and non-specific for bar was developed. Additionally, a set of markers that can detect both pat and bar was developed, and the quantitative PCR data indicated that the primer pairs were sensitive enough to detect 0.1% of the mixed seed content rate.

• Korean Journal of Environmental Agriculture
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• v.29 no.3
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• pp.287-292
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• 2010

We aimed to investigate the responses of soil organisms to cover plants and to provide information for the selection of proper plant species. We studied the effects of 7 cover plants, including rye, oat, rattail fescue, Chinese milk vetch, red clover, crimson clover, and hairy vetch, on soil organisms in an apple orchard. An increase in the microbial phospholipid fatty acids (PLFA) and in the number of nematodes and microarthropods in the soil under the cover plants reflects elevated activities of soil organisms. A decrease in the level of some marker PLFA, which is an indicator of environmental stress, suggests that cover plants provide favorable environments for soil organisms. The population of fungi and animals that feed on fungi increased in the soil surface under red clover. The population density of nematodes and mites increased in the soil surface under rattail fescue, and that of mites and omnivorous nematodes increased in the soil surface under Chinese milk vetch. The level of microbial PLFA in the soil surfaces under the tested cover plants was higher than that under clean culture system. These results suggest that proper selection of the cover plants can facilitate the creation of favorable environments for soil organisms.

• Korean Journal of Environmental Biology
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• v.34 no.4
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• pp.246-256
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• 2016

The objective of the study was to investigate vascular plants of Mueuido in Incheon from April to October, 2011. The investigation was carried out for mountains (Mt. Horyonggok and Mt. Guksa), coastal areas and annexed islands (Silmido and Somueuido). Vascular plants were found to have 338 taxa, including 90 families, 235 genera, 297 species, 5 subspecies, 32 varieties and 4 forms. Rare and endangered plants designated by Korea Forest Service had 5 taxa including Utricularia bifida, U. vulgaris var. japonica, U. racemosa, Ottelia alismoides, and Arisaema heterophyllum. The floristic regional indicator plants found in this area had 3 taxa of grade V and 5 taxa of grade III. Naturalized plants consisted of 20 taxa that made up 8.3% of the total vascular plants in this area.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.3 no.3
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• pp.12-19
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• 2000

The purpose of this study is to evaluate the channel changes according to the temporal and spatial distribution of the deposition area by the vegetation invasion in Kyesung-river. The deposition area mainly occurred by landslide and debris flow from the headwater channel. And also the movement of subsequent downstream depends upon the site of deposits by a varity erosional processes. As the age of deposition area is older, it had a tendency to stable by plant invasion relatively. The vegetations grown in deposition area were very effective to estimate a historical deformation process of river-bed occurred by landslide. The vegetations around deposition area consisted of the same as tree species grown in forest area of circumference like Pinus densiflora, Styrax japonica, Quercus acutissima and Salix gracilistyla. If the torrential stream is flooding, the deposition area of 1 to 5 years can be change to the channel easily. Deposition area of 11 to 23 years had a high river-bed because it passed long time since deposited, and amount of sedimention is much more in wide than in narrow channel. It is consider that the change of channel had many influenced by the span of survial time, scale and movement frequency of deposition area after the vegetation invasion.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.19 no.4
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• pp.381-389
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• 2009

Confined spaces are inherently dangerous workplace and many fatal and nonfatal accidents have been reported. Even though these accidents occur in various kinds of confined spaces, there has rarely been reported on the health hazard agent, i.e., the types of gases and their concentration ranges. Therefore in this study, we evaluated several toxic and asphyxiating gas concentrations in various confined spaces. We surveyed 57 manholes, 3 sewage treatment plants, 2 yellow radish manufacturing companies and 7 barges to measure the concentrations of oxygen($O_2$), hydrogen sulfide($H_2S$), carbon monoxide(CO), ammonia($NH_3$). Lower Explosion Limits(LEL) and Volatile Organic Compounds (VOCs). Those four types of confined spaces occupies 56% of accidents during last 9 years in Korea. In 57 manholes, the concentration varied according to the types of manholes; rainfall and sewage, and by location; residential and industrial areas. Sewage manhole in industrial area was evaluated as the most hazardous than other types of manhole like rainfall manholes, residential sewage manholes. The highest $H_2S$ concentration and the lowest $O_2$ concentration at sewage manhole in industrial area were 300 ppm, 8.7% respectively. In 3 sewage treatment plants, $H_2S$ and $NH_3$ concentrations were reached up to the 500 ppm and 200 ppm respectively. Two yellow radish manufacturing companies showed the concentrations of 316 ppm, 505.2 ppm, 90 ppm and 15.7% for $H_2S$, CO, VOCs and $O_2$, respectively. Seven barges showed 15.9%~20.9% oxygen concentration. Gas species and concentration ranges varied by the types and location of confined spaces; CO, $H_2S$, $O_2$ could be hazardous in manhole, especially manhole connected to sewage plants. CO, $H_2S$, LEL, $O_2$, $NH_3$ should be controlled in sludge silo and sluge pumping confined spaces in sewage treatment plant. The activity of lifting out radish from the storage tank was evaluated more hazardous rather than the other activities in yellow radish manufacturing industry. The employers must conduct the survey to identify all possible confined spaces in their local workplace prior to performing the tasks. At the national level to reduce the accidents in the confined spaces, we suggest that systemic approach and active education program including possible hazards, standard operation procedures, ventilation plan, and personal protective equipment in confined spaces should be implemented.

• Journal of the Korean Society of Radiology
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• v.10 no.1
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• pp.53-58
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• 2016

March 11, 2011 Fukushima nuclear power plant accident has occurred, the last four years, his is the current state of anxiety remains on the consumer. Therefore, 2015 the current progress continues to have continued research for the purpose of resolving the insecurity of fishery in the country. We measured the radiation of the origin of fishery products, by 2015, research was carried out through a number of references to get additional data or studies that influence this to some degree. Create a consumer and one for the seller for the survey to find out the thoughts of both consumers and sellers on fisheries was to create the statistics by direct survey in 2014 year survey meter using PM1405 equipment dynamics of several species of aquatic origin in accordance with the (Taiwan, China, Russia), mackerel, pomfret, hairtail, saury, shrimp, squid measurements of radiation were investigated. The measurement in 2014 was $0.043{\sim}0.073{\mu}Sv/h$. The seller influenced the survey (90%) on fishery products sales, consumer safety, without this fishery (90%), radioactive contamination very high (28%) and the polarization was not as nearly 72%. The study of the stability of the radiation determined by the radiation levels of the aquatic marine products in a comparison the radiation levels result was determined in ICRP below the standard value (1 mSv/y).

• Korean Journal of Weed Science
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• v.32 no.3
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• pp.263-272
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• 2012

The potential as natural herbicide of d-limonene natural agent was conducted on several weeds in a greenhouse and Sciyos angulatus in field condition. Herbicidal activity of foliar application at a concentration of 100 and 200 kg ai $ha^{-1}$ of d-limonene on seven weed species was completely killed in a greenhouse condition. Also, d-limonene at a concentration of 50 kg ai $ha^{-1}$ was completely killing on Abutilon theophrasti, Aeschynomene indica, Echinochloa crus-galli and Digitaria ciliaris 3 days after treatment. While pre-emergence treatment of d-limonene concentration of 12.5 to 200 kg ai $ha^{-1}$ showed not significant visible damages. Phytotoxic symptoms of d-limonene by foliar treatment were characterized by wilting and burn-down of leaves and stems followed by discoloration, finally, plant death. Crop selectivity at d-limonene concentration of 100 kg ai $ha^{-1}$ over to five main crops including Zea mays by foliar application was not at all. Field trial of foliar treatment with d-limonene 70 to 140 kg ai $ha^{-1}$ have effectively controled over 5~20 leaf stages of S. angulatus at the natural habitats. And herbicidal efficacy of foliar application on S. angulatus with carrier volume in field condition was increased with dose dependent manners. These results suggest that d-limonene is considered possible as herbicide, and may be further optimized for natural agent for environmental friendly natural herbicide.

• Weed & Turfgrass Science
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• v.2 no.4
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• pp.402-407
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• 2013

Bermudagrass (Cynodon dactylon.) is one of the most difficult weedy species to control in turfgrass because it's high tolerant to various environmental and management stresses. This experiment was performed to find the integrated weed management including cultural practices to suppress bermudagrass in Zoysiagrass matrella (L) Merr. As results, two sequential applications of Fluazifop-P-butyl 0.05 ml $m^{-2}$ + Triclopyr-TEA 0.5 ml $m^{-2}$ and Fenoxaprop-P-ethyl 0.1 ml $m^{-2}$ + Triclopyr-TEA 0.5 ml $m^{-2}$ applied on 20 days intervals were evaluated the primary option for bermudagrass suppression and turfgrass injury was acceptable in zoysiagrass. In both treatments, turf injury was observed during 30days after the first application and almost recovered at 40days. While Fenoxaprop-Pethyl 0.1 ml $m^{-2}$ + Triclopyr-TEA 0.5 ml $m^{-22}$ were lightly phytotoxic to zoysiagrass in chlorophyll content test, there was no growth inhibition of zoysiagrass. Verticut practice (4 mm depth) just before herbicides application where zoyisagrass is contaminated with bermudagrass was not helpful to reduce turf injury in this experiment. However, alone verticut management was utilized to decrease about 12-14% bermudagrass population. Thus the application of Fenoxaprop-P-ethyl 0.1 ml $m^{-2}$ + Triclopyr-TEA 0.5 ml $m^{-2}$ which are permitted for turfgrass after zoysiagrass is perfectly recovered from turf injury by verticut practice should be utilized for bermudagrass reduction in zoysiagrass.