• Title/Summary/Keyword: plant roots

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Effects of Inoculation of Nodule Bacteria (Rhizobium meliloti) on the Nodule Formation and Plant Growth of Alfalfa (Medicago sativa L.) (근류균(根瘤菌)(Rhizobium meliloti)의 접종(接種)이 Alfalfa의 근류형성(根瘤形成) 및 초기생육(初期生育)에 미치는 영향(影響))

  • Kim, Choong Soo
    • Korean Journal of Agricultural Science
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    • v.6 no.2
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    • pp.105-111
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    • 1979
  • This experiment was carried out to define the affinity of alfalfa varieties to nodule bacteria and the effects of nodule bacteria on the growth of alfalfa varieties. The results obtained are summarized as follows; 1. The ability of nodule formation on the roots of alfalfa was significantly different among the strains of nodule bacteria, Rhizobium meliloti SU 47 formed nodules on the roots of two alfalfa varieties, Luna and Von arnims altd bost. Nodules were not formed at the roots of all varieties by inoculation of Rhizobium meliloti M1 but a lot of nodules were formed at the roots of all varieties by inoculation of Rhizobium meliloti M14. 2. When the Rhizobium meliloti M14 was inoculated in the media, the largest numbers of nodules were formed on the roots of alfalfa cultivar Franks hangmeiler and the least numbers of nodules were formed on the roots of cultivar Elga. 3. The plant height and the dry matter weight of all alfalfa cultivars were significantly increased by inoculation of Rhizobium meliloti M14, and also those were increased by applying nitrogen, but when the alfalfa cultivars were grown on the media inoculated Rhizobium M1, the plant height and the dry matter weight of alfalfas were not increased and they were same as those grown on check media. 4. The significant correlation were not detected between the number of nodules formed on the, roots of alfalfa cultivars and the plant height or the dry matter weight of alfalfa cultivars.

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Changes of Organic Solutes and Antioxidative Enzyme Activity in Rice Seedling under Salt Stress

  • Park So-Hyeon;Sung Jwa-Kyung;Lee Su-Yeon;Lee Ju-Young;Jang Byoung-Choon;Song Beom-Heom;Kim Tae-Wan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.5
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    • pp.325-331
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    • 2005
  • Seedlings of two rice genotyopes, cvs. Ilpumbyeo and Gancheokbyeo, were exposed to 0, 50 and 100 mM NaCl in nutrient solution for nine days. Plants were collected at the interval of 3 days and organic and inorganic solutes in leaves and roots and antioxidative enzyme activity in leaves were determined. Under salinity, the accumulation of soluble sugars occurred considerably in the older leaves of stressed seedlings compared to younger leaves and roots. The endogenous Na+ contents markedly increased at higher NaCl concentration in leaves and roots of seedlings, though it was higher accumulated in roots. Salinity resulted in an excessive proline accumulation in the stressed plants. A more pronounced increase was observed in Gancheokbyeo leaves. SOD activity in Impumbyeo cannot found any remarkable change, whereas, in Gancheokbyeo, its activity was rapidly decreased. CAT and POD activities increased with an increase in NaCl concentration in both genotypes. In sum­mary, the high capacity of rice seedlings to overcome an unfavorable growth condition such salt stress appears to be related to an adequate partition of organic solutes between shoots and roots and to changes in absorption, transport and re-translocation of salts.

Varietal Difference in Root Distribution of Direct Seeded Rice

  • Kim, Hyun-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.43 no.1
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    • pp.38-43
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    • 1998
  • This study examined root growth and distribution under the direct seeding of rice cultivars developed in Korea, Japan, and the U.S. Cultivars from the U.S., especially 'M202' and 'Caloro', had a high ratio of the dry matter partitioning for root and top plant components. Caloro had high root and top growth. 'Koshihikari' from Japan showed the lowest ratio of R/T (root/total dry matter) due to the small amount of root and top plant growth. Most Japanese cultivars except transplanted 'Hatsuboshi' showed low ratio of R/T. Patterns of root distribution for each soil block were recorded by the root box-pin board method. Roots of all cultivars were distributed in blocks A, C, and E in the middle of box, i.e., just below the plant base. Roots of 'Dongjin', M202, and Caloro were distributed deeper than the others. Roots of transplanted Hatsuboshi developed much better than direct seeded Hatsuboshi. Total root weight density was highest in Caloro followed by Dongjin, 'Gancheok', 'Calrose', and the others. The root density of Caloro was twice as much as those of the others except Dongjin. According to cumulative percentages of root distribution on each soil layer, roots of most cultivars were distributed below 20cm. The U.S. cultivars showed vertically well developed root systems as compared to others. A large amount of roots were distributed in the top 15cm of soil layer for Hatsuboshi and Koshinikari, and their root systems appeared to be shallow. In contrast, the rates of root distribution in the top 10cm of soil layer were low for Dongjin, Calrose, and Caloro. These cultivars had relatively deep root systems.

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Ginsenosides Production through in vitro Culture of Adventitous Roots Induced from Panax ginseng "Chunpoong" (인삼 천풍의 부정근 배양을 통한 Ginsenosides 생산)

  • 인준교;이범수;송원섭;양덕춘
    • Korean Journal of Plant Resources
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    • v.17 no.1
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    • pp.7-13
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    • 2004
  • We have investigated the optimal conditions for the growth and ginsenoside production in adventitious root of “Chunpoong” (Panax ginseng C.A. Meyer). Ginseng adventitous roots induced from the embryo of “Chunpoong” were cultured in various plant media supplemented with several growth hormones. The best growth rate and high ginsenoside contents were obtained in B5 medium supplemented with IBA (2 mg/L) and kinetin (0.1 mg/L). The supplement of 2.5 mM KH$_2$PO$_4$ was good for high growth rate of the adventitious roots, but the accumulation of ginsenosides was increased by reducing the KH$_2$PO$_4$ concentration to 1.25 mM. We have established the effective liquid culture system for the optimal growth and ginsenoside production of “Chunpoong” adventitious roots.

Biological Effects of the Leaves and Roots of Ligularia stenocephala (곤달비 잎과 뿌리의 생물 활성)

  • Nam, Young-Joo;Lee, Dong-Ung
    • Journal of Life Science
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    • v.23 no.11
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    • pp.1381-1387
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    • 2013
  • The leaves and roots of Ligularia stenocephala, which are widely used as a food in Korea, were investigated for their antioxidant activities and cytotoxicity in vitro, and their hepatoprotective effect, alcohol detoxicant efficacy, and memory-enhancing property were investigated in vivo. The unique odor of the leaves was analyzed by GC-MS. Lipid peroxidation, superoxide anion formation, and DPPH radicals were inhibited remarkably by the extracts of the leaves and roots. The leaves of this edible plant significantly protected the hepatotoxicity induced by carbon tetrachloride and further diminished the blood alcohol content in mice. While the roots of this plant exhibited adequate cytotoxicity against four human tumor cell lines, especially against melanoma, the leaves revealed relatively weak activity. Both the leaves and the roots exerted an excellent ameliorating property on scopolamine-induced memory impairment in the passive avoidance task using an animal model. The hexane fraction of the leaves was analyzed by GC-MS, suggesting that a series of terpenoids may be odorous compounds in this plant.

Sansam of South Korea (한국 산삼의 형태학적 연구)

  • Shin Soon Shik;Kim Gyeong Cheol;Kim Chang Shik
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.16 no.6
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    • pp.1260-1262
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    • 2002
  • A particular type of ginseng that grows in mountainous regions of Korea is known as Sansam, a term meaning literally mountain grown ginseng. Sansam has recently gained a reputation among some Korean people who believe its health benefits are superior to that of other types of ginseng. Misuse of Sansam has resulted from misinformation that has circulated about the health benefits of its use. Due to insufficient study and research, Korean Oriental Medicine academia does not presently have enough credible information about Sansam to properly educate the public in its use. However, we do have a responsibility to perform adequate study and research so that correct information may be provided. To date, only cursory investigation of the physical characteristics of the Sansam plant have been conducted. This limited investigation was performed in July 2002, at Sobaek Mountain. The branches, leaves, stems, peduncles, fruits, and roots (head, main and fine roots) were observed. The fine roots grew and spread in a large area around the main roots so that harvesting the plants required digging out the soil a considerable distance from the main roots. The plants grew in a northwesterly direction, with a gradient of 40 degrees. They had four branches. Three of the branches had six leaves, while one had five leaves. Each plant had 40 fruits. The roots of the plants grew in a shape similar the Chinese character for mountain. The roots were milky in color. The average weight of the plants was 42.5 grams.

Comparison of Glucosinolate Contents in Leaves and Roots of Radish (Raphanus spp.)

  • Ko, Ho-Cheol;Sung, Jung-Sook;Hur, On-Sook;Baek, Hyung-Jin;Jeon, Young-ah;Luitel, Binod Prasad;Ryu, Kyoung-Yul;Kim, Jung-Bong;Rhee, Ju-Hee
    • Korean Journal of Plant Resources
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    • v.30 no.6
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    • pp.579-589
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    • 2017
  • Glucosinolates (GSLs), beneficial secondary metabolites for human health are abundantly present in radish vegetable. Radish is a member of Brassicaceae family and its seed, leaf and root contain very important GSLs. The objective of this study was to determine the variation of individual and total GSL contents in leaves and roots of 44 radish (Raphanus spp.) germplasm (26 R. sativus L., 3 R. raphanistrum, and 15 R. sativus L. var. raphanistroides Makino), and compare the GSL contents between leaves and roots among three Raphanus species. Thirteen GSLs were identified, being the glucoraphasatin (GRS) and glucobrassicin (GBS) the most abundant aliphatic and indolyl GSLs in both the leaves and roots. Variation in individual and total GSL contents was found among the germplasm of three Raphanus species. The GRS content was higher in roots than that of leaves in all three Raphanus species but the GBS content was higher in leaves than roots. GRS was represented 87.0%, 92.7% and 94.7% of the total GSL in roots of R. sativus L., R. raphanistrum and R. sativus L. var. raphanistroides (Makino) germplasm, respectively. Germplasm of R. raphanistrum exhibited the highest (average, $79.5{\mu}mol/g\;dw$) total GSL with a ranged from 62.7 to $92.9{\mu}mol/g\;dw$. The germplasm IT119288, Joseonmu and IT119262 from R. sativus L., RA 504 and K046542 from R. raphanistrum, and Gyeongju-2003-32 (G2003-32) and IT302373 from R. sativus L. var. raphanistroides (Makino) had high total GSL contents and these could be good candidates for developing the functional compounds-rich varieties in radish breeding program.

A study on pearl millet (Pennisetum glaucum L.) plant Biochemical and histochemical changes inoculated with indigenous AM fungi under Barren soil

  • Pal, Ajay;Pandey, Sonali
    • Journal of Plant Biotechnology
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    • v.44 no.2
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    • pp.203-206
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    • 2017
  • The soil organisms that develop beneficial Symbiotic relationships with plants roots and contribute to plant growth are mycorrhizal (AM) fungi. Arbuscular mycorrhizal inoculations change the growth and biochemical composition of the host plant and soil. Mycorrhizal root systems do augment the absorbing area of roots from 10 to 100 times thereby greatly improving the ability of the plants to utilize the soil resources. A pot experiment was conducted during the kharif seasons at Jaipur, Rajasthan, to find out the effects of three different indigenous AM fungi i.e. Glomus mosseae, Glomus fasciculatum and Gigaspora decipiens either single and in combination inoculation on biochemical and histochemical changes of Pearl millet (Pennisetum glaucum L.) grown under barren soil conditions. The AM fungus has shown to improve the tolerance of plant to drought stress. Experimental results showed that AM fungi treated plants improved their plants growths, biochemical and histochemical changes as compared to non-mycorrhizal treatments. The AM fungi inoculated plant was found to be attaining maximum plant biochemical and histochemical substances in Glomus mosseae (alone) and also Glomus mosseae + Glomus fasciculatum treatments.

Plant Regeneration and Bulblet Formation of Allium wakegi Araki

  • Song, Won-seob;Yang, Deok-Chun;Yoon, Jae-Ho;Ryu, Sang-Hyun
    • Plant Resources
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    • v.7 no.1
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    • pp.7-14
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    • 2004
  • Allium wakegi was cultured shoot tip in the condition of light culture. The Allium wakegi added plant growth regulator was observed of plant regeneration and bulblet formation. Callus Induction and growing rate was the best of 78% when added alone 2,4-D 0.5mg/L. In the formation of shoot, its regeneration rate was 96% when added BA 0.5mg/L in the light culture condition. When BA 0.5mg/L and NAA 0.5mg/L mixed and BA 0.5 mg/L and NAA 1.0mg/L mixed, the rates were 99% and 97% respectively, and these conditions were suitable for forming shoot. In the formation of roots, when added NAA 2.0mg/L in the light culture condition, the regeneration rate was 90.6 % and the roots were abnormal. When added NAA 1.0mg/L, the rate was 82 % and the highest. In the formation of bulbs, when BA 05mg/L and NAA 1.0mg/L mixed, the root generantion and its size in the bulbs was the best compare to other treatment experiments.

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High-performance liquid chromatography analysis of phytosterols in Panax ginseng root grown under different conditions

  • Lee, Dong Gu;Lee, Jaemin;Kim, Kyung-Tack;Lee, Sang-Won;Kim, Young-Ock;Cho, Ik-Hyun;Kim, Hak-Jae;Park, Chun-Gun;Lee, Sanghyun
    • Journal of Ginseng Research
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    • v.42 no.1
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    • pp.16-20
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    • 2018
  • Background: The Panax ginseng plant is used as an herbal medicine. Phytosterols of P. ginseng have inhibitory effects on inflammation-related factors in HepG2 cells. Methods: Phytosterols (e.g., stigmasterol and ${\beta}$-sitosterol) in the roots of P. ginseng grown under various conditions were analyzed using high-performance liquid chromatography. The P. ginseng roots analyzed in this study were collected from three cultivation areas in Korea (i.e., Geumsan, Yeongju, and Jinan) and differed by cultivation year (i.e., 4 years, 5 years, and 6 years) and production process (i.e., straight ginseng, red ginseng, and white ginseng). Results: The concentrations of stigmasterol and ${\beta}$-sitosterol in P. ginseng roots were 2.22-23.04 mg/g and 7.35-59.09 mg/g, respectively. The highest concentrations of stigmasterol and ${\beta}$-sitosterol were in the roots of 6-year-old P. ginseng cultivated in Jinan (82.14 mg/g and 53.23 mg/g, respectively). Conclusion: Six-year-old white ginseng and white ginseng cultivated in Jinan containing stigmasterol and b-sitosterol are potentially a new source of income in agriculture.