• Asian-Australasian Journal of Animal Sciences
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• v.31 no.7
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• pp.933-950
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• 2018

In Japan, Wagyu cattle include four Japanese breeds; Black, Brown, Shorthorn, and Polled. Today, the renowned brand name Wagyu includes not only cattle produced in Japan, but also cattle produced in countries such as Australia and the United States. In recent years, the intramuscular fat percentage in beef (longissimus muscle) from Japanese Black cattle has increased to be greater than 30%. The Japanese Black breed is genetically predisposed to producing carcass lipids containing higher concentrations of monounsaturated fatty acids than other breeds. However, there are numerous problems with the management of this breed including high production costs, disposal of untreated excrement, the requirement for imported feed, and food security risks resulting from various viral diseases introduced by imported feed. The feeding system needs to shift to one that is more efficient, and improves management for farmers, food security for consumers, and the health environment for residents of Japan. Currently, we are developing a metabolic programming and an information and communications technology (ICT, or Interne of Things) management system for Wagyu beef production as future systems. If successful, we will produce safe, high-quality Wagyu beef using domestic pasture resources while solving the problems of how to utilize increasing areas of abandoned agricultural land and to make use of the plant-based feed resources in Japan's mountainous areas.

• Journal of Microbiology and Biotechnology
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• v.16 no.12
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• pp.1862-1867
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• 2006

A moderately halophilic, Gram-positive, spore-forming bacterium was isolated from the roots of Blutaparon portulacoides, a plant found in sandy soil parallel to the beach line in Restinga de Jurubatiba, Rio de Janeiro, Brazil. The strain, designated $M9^T$, was motile and strictly aerobic with rod-shaped cells. It grew in the absence of NaCl and up to 20% NaCl, and was able to hydrolyze casein and starch. Strain $M9^T$ had a cell-wall peptidoglycan based on L-Orn-D-Asp, the predominant menaquinone present was menaquinone-7 (MK-7), diaminopimelic acid was not found, and anteiso-$C_{15:0}$ and iso-$C_{15:0}$ were the major fatty acids. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain $M9^T$ belonged to the genus Halobacillus and exhibited 16S rRNA gene similarity levels of 97.8-99.4% with the type strains of the other nine Halobacillus species. The DNA-DNA relatedness of strain $M9^T$ with H. trueperi, the closest relative as regards 16S rRNA gene similarity, and H. locisalis was 21% and 18%, respectively. Therefore, on the basis of phenotypic, genotypic, and phylogenetic data, strain $M9^T$ (=ATCC BAA-$1217^T$, =CIP $108771^T$, =KCTC $3980^T$) should be placed in the genus Halobacillus as a member of a novel species, for which the name Halobacillus blutaparonensis sp. nov. is proposed.

• The Korean Journal of Pharmacology
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• v.19 no.2
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• pp.17-24
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• 1983

Buxus leaves(Buxus microphylla var. koreana Nakai) have been used as a folk medicine in treating woman's disease. The chemical constituents, which have teen identified from Buxus sempervirens L. in Europe, are so far known in isolation of buxus alkaloids, cyclobuxine, buxamine, norbuxamine and buxaminol. But But any study on the pharmacological action of Buxus microphylla var. koreana Nakai has not yet teen rallied out, however, active substances have not been identified. As an attempt to isolate the biologically active substances from this plant, the examination of chemical constituents was undertaken. In the experiment, the crystalline component$(C_{10}H_8O_4)$ obtained from Buxus microphylla var. koreana Nakai was identified as a 6-methoxy, 7-hydroxy coumarin by chemical and physical methods. We named this crystal buxuletin. Therefore, we intended to screen the pharmacological action of buxuletin, especially, its diuretic effects in rabbits. Buxuletin was intraveneously injected to the rabbit in the dose of 20 mg/kg, 10 mg/kg and 5 mg/kg. The variations of urine volume and minerals $(Na^+,\;K^+\;and\;Cl^-)$ in urine were measured at an interval of 15 minutes for 4 hours after the treatment of buxuletin. The observed results are as follows: 1) With the administration of buxuletin (20 mg/kg, 10 mg/kg), the remarkable increment of the excreted urine volume was observed during the relatively long period. Excretion rates of urinary minerals $(Na^+,\;K^+\;and\;Cl^-)$ were also increased. 2) In a dose of 5 mg/kg, the slight increment on the diuretic actions of rabbits was observed. From the above results, authors name this crystal buxuletin and buxuletin shows the diuretic action.

• Korean Journal of Oriental Medicine
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• v.9 no.1
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• pp.81-89
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• 2003

Polygoni multiflori Radix has been used as a tonic medicine. In Korea, Cynanchi wilfordii Radix have been used too. Their names are resembled, but their plant origines are different. Polygoni multiflori Radix is called 何首烏 or 赤何首烏, and Cynanchi wilfordii Radix is 白何首烏 or 白首烏. They are suggested that they had been confused using at the early days in drug history. Polygoni multiflori Radix is enclosed in the pharmacopoeias of Korea, North Korea, Chinese and Japan. The nomina of pharmacopoeias are 赤何首烏 at North Korea and 何首烏 at other countries; Korea, Chinese and Japan. Cynanchi wilfordii Radix is just enclosed in Korea and North Korea. It means that Cynanchi wilfordii Radix has been commonly prescribed in Korea and North Korea than other countries. The nomina of pharmacopoeias are 白首烏 in Korea and 白何首烏 in North Korea. The characteristics of 何首烏 in ancient herbal records are confused of Polygoni multiflori Radix and Cynanchi wilfordii Radix. But Polygoni multiflori Radix is fixed at 何首烏 later. In Korea (south and north) Cynanchi wilfordii Radix has been recorded to using in 東醫寶鑑 that was Korea traditional Medicinal book and wrote at 1613. The 白首烏 is named in chinese about 20 century, but 白何首烏 is in korea about 19 century. In these consequences, prescription of Cynanchi wilfordii Radix in Korea is earlier than Chinese and Japan, and more common consumption too. So the nomen of 白何首烏 is better properly than 白首烏 in Korean Herbal pharmacopoeia.

• Journal of the Korean Society of Food Culture
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• v.14 no.1
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• pp.1-15
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• 1999

A survey was conducted in 591 college students using 8 different cafeterias serving 31 meals. The information was obtained about the name and amount of dishes they consumed from the menu offered by college foodservice. Mean nutrient contents per meal in offered menu were higher than 1/3 of RDA for their age, sex in offered menu. The mean energy content was 466kcal from rice, 113kcal from soup, 141kcal from side dish and 21kcal from kimchi. When side dishes were classified by cooking methods, side dishes using grilling, frying, roasting methods had high energy content and fat percent above 200kcal, 40% per dish. By main ingredients, side dishes with animal food were higher energy than with plant food. Students consumed 94% of the energy provided in offered menu. When compared to proportion of foods consumed by sex, there was 43% of female and 22% of male consumed less than half in soup and 31% of female and 12% of male in kimchi. The most prevalent menu patterns of subjects included rice, soup, 2 side dishes, kimchi and were the same in both sexes. Mean energy intakes per meal were 989kcal for males and 842kcal for females which were enough to meet 1/3 of RDA for their age and sex. Most nutrient intake except fat and vitamin B1 were higher in set menu than in cafeteria. Nutrient adequacy ratio(NAR) were above 0.9 except calcium and vitamin A. The mean energy intake was 542kcal from rice, 70kcal from soup, 164kcal from side dish and 20kcal from kimchi. In conclusion, intake of most of the nutrients of students obtained from males in college foodservice were sufficient but calcium intake was insufficient and fat content was above 20% of energy. High fat consumption was due to side dishes from frying, grilling, roasting with animal food. To provide desirable meals in college foodservice, use of fat in cooking must be decreased.

• The Korea Journal of Herbology
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• v.26 no.3
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• pp.57-63
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• 2011

Objectives : Peucedani Radix ('Jeonho' in Korean) is the root of Peucedanum praeruptorum or Angelica decursiva. However, the root of Anthriscus sylvestris has usually been distributed as Jeonho. This study was performed to determine the discriminative criteria of Jeonho, focused on distribution in Korean markets. Methods : We have determined identification keys to discriminate each Jeonho samples, through observation of external morphology of original plants, and the research of external and internal morphological features of dried Jeonho herbs. Results : Because of identical to the Korean plant name 'Jeonho', Anthrisci Radix had been came into use as a substitute of Jeonho in Korea during the time of Japanese colonial rule. The original plants of Jeonho and its substitute are discriminated with shape of leaf, leaf margin and color of stem. External morphological features of the medicinal herbs of Jeonho are different in the color of cross-sections, pellucid dot, white powder. Internal morphological points, such as fiber bundle of xylem, seconadary mudullary ray and ray of xylem were also used as discriminative criteria for Jeonho. Further details(e.g. identificaion keys) are in the article. Conclusions : We think that these discriminative criteria will be meaningful in identifying the substitutes and adulterants of Jeonho.

• Mycobiology
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• v.45 no.3
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• pp.178-183
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• 2017

Diabetes mellitus is a chronic disorder which affects millions of population worldwide. Global estimates published in 2010 reported the world diabetic prevalence as 6.4%, affecting 285 million adults. Foot ulceration and wound infection are major forms of disabilities arising from diabetic diseases. This study was aimed to develop a natural antimicrobial finishing on medical grade textile that meets American Association of Textiles Chemists and Colorists (AATCC) standard. The textile samples were finished with the ethanolic extract of Penicillium amestolkiae elv609, an endophytic fungus isolated from Orthosiphon stamineus Benth (common name: cat's whiskers). Endophyte is defined as microorganism that reside in the living plant tissue, without causing apparent disease symptom to the host. The antimicrobial efficacy of the ethanolic extract of P. minioluteum was tested on clinical pathogens isolated from diabetic wound. The extract exhibited significant inhibitory activity against 4 bacteria and 1 yeast with the minimal inhibitory concentration ranged from 6.25 to 12.5 mg/mL. The results indicate different susceptibility levels of the test microorganism to the ethanolic extract. However, the killing activity of the extract was concentration-dependent. The finished medical textile showed excellent antimicrobial efficacy on AATCC test assays. All the microbial cultures treated with the textile sample displayed a growth reduction of 99.9% on Hoheinstein Challenge Test. The wash durability of the finished textile was found good even after 50 washes with commercial detergent. Besides, the gas chromatography mass spectrometry analysis showed that 6-octadecenoic acid and diethyl phthalate were the main bioactive constituents of the extract. In conclusion, the developed medical textile showed good antimicrobial efficacy on laboratory tests. This work can be extended to in vivo trials for developing healthcare textile products for antimicrobial applications.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.32 no.2 s.57
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• pp.69-74
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• 2006

Selaginella tamariscina with the popular Korean name Keoun Back, is a traditional medicinal plant for therapy of advanced cancer patients in the Orient. In this study, we evaluated anti-aging activity of S. tamariscina using processed technology and investigated diverse biological activities of processed S. tamariscina (PST) as an anti-aging ingredient of cosmetics. PST, heated with sand, used to different purpose compared with origin in medicine. PST raises total phenol concentration and enhances the DPPH radical scavenging activity. For testing intracellular ROS scavenging activity, the cultured human dermal fibroblasts were analyzed by increase in dichlorofluorescein (DCF) fluorescence upon exposure to UVB $20 mJ/cm^2$ after treatment of PST. UVA-induced MMP-1 expression in human dermal fibroblasts was reduced in a dose-dependent manner by PST. Taken together, 4hese results suggest that PST may act as an anti-aging agent by preventing the skin cell from damage induced by UV irradiation, and imply that PST may be useful as a new ingredient for anti-aging cosmetics.

• Journal of Haehwa Medicine
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• v.14 no.1
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• pp.15-22
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• 2005

Source : sources used by Qianghuo is Ostericum koreanum Maximowicz, Notopterygium incisum Ting and Notopterygium forbesii Boissier etc. in Korea and China. And we cirulate a Qianghuo dividing Nanqianghuo and Beiqianghuo. Thought source of Korea Qianghuo is different from china's we use a same name. And source of Beiqianghuo has not made clear yet. According to research accomplished 2002, we have to use Nanqianghuo producted at Jinbu in aspect of aspect. but, Beiqianghuo the outside form of Beiqianghuo similar to china so we think that we have to more make clear source of domestic Qianghuo, verify a source Beiqianghuo and re-establish about effects and functions of domestic Qianghuos. Culture : A term of culture is an nearly one year. so to product Qianghuo of good quality, we must plant directly in autumn or raise seedlings and transplant in spring. Processing : To protect turning to brown, Qianghuo are cirulate mostly as the original form without washing in water, To proteet crushing of Qianghuo when we cut off, Qianghuo were cut off after being soaked in water or steamed by heat. So when we wash and cut off, it has to be made processes which have no decreases of medical effects and be suggested a proper index which verify a medical effects. In summury, after harvesting Qianghuo the first washing $\rightarrow$ the first dry $\rightarrow$ selection $\rightarrow$ the second washing $\rightarrow$ the second dry $\rightarrow$ selection $\rightarrow$ cutting off $\rightarrow$ the third dry.

• The Korea Journal of Herbology
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• v.32 no.1
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• pp.41-45
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• 2017

Objectives : The purpose of this study was to assume the origin and daily dose of Allii Chinensis Bulbus in Treatise on Cold Damage Diseases and Prescriptions of the Gold Chamber. Methods : I assumed the origin with Book of Materica Medica such as Illustration and Classic of Materia Medica(本草圖經), Illustration and Consideration of Plant's Name and Reality(植物名實圖考), The Korean Herbal Pharmacopoeia Forth Edition, Annotation of Hangeul Treatise of Cold Damages Diseases, and daily dose based on general principle applied on dose of Treatise on Cold Damage Diseases. Results : In Treatise on Cold Damage Diseases and Prescriptions of the Gold Chamber, the original species of Allii Chinensis Bulbus was Allium chinense G. Don. It was to be measured with weight. It was too big to write on size due to it's 10~30mm length and 3~12mm in diameter. And due to irregular size, it can't be written on number, too. Daily dose of Allii Chinensis Bulbus in Whaeyeok-san(回逆散), Gwalluhaebaekbanha-tang(栝樓薤白半夏湯) was 3 Ryang(兩), which was the most common case in Treatise on Cold Damage Diseases. And that in Gwalluhaebaekgyeji-tang(栝樓薤白桂枝湯), Gwalluhaebaekbaekju-tang(栝樓薤白白酒湯) is 8 Ryang(兩), which was the largest dose as dried herb in Treatise on Cold Damage Diseases Conclusions : The original species of Allii Chinensis Bulbus was Allium chinense G. Don. The daily dose of Allii Chinensis Bulbus was 3 Ryang(兩)(19.5g) in Whaeyeok-san and Gwalluhaebaekbanha-tang, and was 8 Ryang(兩)(52g) in Gwalluhaebaekgyeji-tang and Gwalluhaebaekbaekju-tang.