• Proceedings of the Korean Society of Organic Agriculture Conference
• /
• 2009.12a
• /
• pp.45-56
• /
• 2009

Current status on the occurrence and the management of the major disease, insect and mite pests were investigated in the organic or non-chemical pest control orchards from 2005 to 2009. Numbers of certified organic or non-chemical apple orchards were increased from 14 in 2005 to 78 in 2008. Severe damages on leaves and fruits occurred by the several diseases such as marssonina blotch, bitter rot, white rot, sooty blotch and flyspeck, and the several insect pests such as apple leaf-curling aphid, woolly apple aphid, oriental fruit moth and peach fruit moth on the almost certified organic or non-chemical pest control orchards. About 10 and 18 environmental-friendly materials were used to control diseases and insect or mite pests respectively. But, lime sulfur and bordeaux mixture to diseases and machine oil, plant oil mixed with egg yolk, and pheromone mating disruptions to insect pests were effective to control under the adequate conditions. At present, it is extremely difficult to produce organic apples in Korea. Growers must consider about and solve so many conditions on the cultivar, weather, local site, marketing and so on, before when they decide to change from conventional or IPM(Integrated Pest Management) to organic or non-chemical pest control orchards.

• Weed & Turfgrass Science
• /
• v.2 no.3
• /
• pp.236-241
• /
• 2013

Introduction of rye living mulch in soybean production is important for labor-saving and reduction of herbicide usage for weeding by weed suppression and for soil conservation by mulching of rye. Soybean and rye were row-planted and broadcasted simultaneously at different planting times (May 15, May 25, June 5) and row widths (35 and 70 cm) in 2010, different soybean cultivars (Daepoong, Singi) and planting times (May 15 and June 1) in 2011, respectively, and investigated weed occurrence, early growth and yield of soybean. Acalypha australis, which is known to herbicide-resistant weed, could be controlled ecologically by rye living mulch. Occurrence of Acalypha australis was controlled effectively also by mixture of alachlor and linulon at no mulch in 2011. Early growth amount of soybean plant per area was increased by narrow row width (35 cm) at rye living mulch, which was helpful in the control of weeds, but grain yield of soybean with narrow row was decreased compared to conventional row width (70 cm) at the planting in early June. It is concluded that soybean planting as row width 70 cm in early June is proper at simultaneous planting with rye.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.7 no.1
• /
• pp.25-30
• /
• 1987

There are differences in opinion as to whether nitrogen fertilizer should be used when establishing alfalfa (Medicago sativa L.). Various reports show that under a hot environment, rhizobia (Rhizobium meliloti) are not as effective in fixing atmospheric nitrogen as they are under moderate temperatures. It is also believed that the addition of nitrogen fertilizer inhibits nodulation of alfalfa seedlings. A replicated experiment was conducted under controlled environmental conditions at the University of Nevada-Reno, Reno, Nevada, USA, to determine the effects of nitrogen application on seedling growth and nodulation of alfalfa grown in a hot environment. Sterile sand was used as the growing media to which a complete nutrient solution minus nitrogen was applied volumetrically to each pot daily. In addition, half of the pots received NH4-$NO_3$, at the rate of 11.2 kg per ha at seeding and at two and four weeks after planting giving a total nitrogen application rate of 33.6 kg per ha during the seven-week experimental period. Rhizobia inoculant (R-12) consisted of a mixture of strains 171-15a, 1682c and 80 PI 265 of (Rhizobium meliloti). Inoculant was applied to the seeds prior to planting and to the sand media at two and four weeks after seeding. Twenty seeds were planted in pots 14.0 cm in diameter and 11.5 cm deep. Plants were thinned to ten plants per pot after emergence and were grown in a controlled environment chamber with a 16-hour light period. Soil temperature at 6 cm depth ranged from 17.4^{\circ}C.$ to 31.1^{\circ}C.$ and had a daily mean of 26.5^{\circ}C.$. Plants were harvested at weekly intervals for seven weeks. Root, shoot and total length, dry weight, volume and number of nodules per plant were determined. Root, shoot, and total length were greater in seedlings grown in soil where nigrogen was applied than that grown in soil to which no nitrogen was applied. The average size of the seedlings as determined by volume and weight was more than two times greater where plants were fertilized with nitrogen. Nodule number per seedling was also greater when nitrogen was applied compared to those which received no nitrogen. The differences were greater as the plants became older. The rhizobia did not fix enough nitrogen for adequate growth of seedlings. This is probably due to high temperature growing conditions that caused the rhizobia to become relatively ineffective as compared to cooler growing conditions. Data suggests it would be desirable to apply nitrogen at seeding when alfalfa is established under hot conditions that occur in mid- or late summer.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.30 no.4
• /
• pp.301-308
• /
• 2010

This study was carried out in the Chungnam National University grassland experimental field from October, 2007 to December, 2009 in order to find out the growth characteristics, seasonal anthesis distribution and botanical composition of the wildflowers which were altogether composed of 70% sheep fescue and 30% introduced wildflowers. The experimental species contained 23 species in total to which sheep fescue (fundamental turfgrass) and 22 introduced wildflowers belonged. At the time of the anthesis of the wildflowers, the average plant height was 29.7 cm at first growing season and 34.1 cm at second growing season. They bloomed out into eight colors, but into simplified two colors during August, September, and October. The blooming season leant toward April and June and from August there were lots of difficulties in the wildflower blooming and the maintenance of their consistency. The botanical composition of wildflowers, sheep fescue, and weeds were 21%, 64%, and 15% in the first growing season (2008) and 19%, 65%, and 16% respectively, in the second growing season (2009). Based on this result, cultivating the introduced wildflower grassland has more difficulty because there is some difficulty in the wildflower seasonal distribution and the maintenance of its consistency. And thus, the introduction of more various wildflower such as perennials and flowers blooming after summer, and the seed-mixture combination method should be studied.

• Journal of Life Science
• /
• v.24 no.4
• /
• pp.386-392
• /
• 2014

Haw (Crataegus pinnatifida $B_{UNGE}$) is a medicinal plant commonly used in Korea due to its beneficial health effects. This study was performed to investigate the antioxidative activity of bioactive components from haw seed. Freeze-dried seed was processed with various solvents such as 70% methanol, 70% ethanol, a mixture of chloroform and methanol (2:1, v / v), n-butanol and ethyl acetate (EA). DPPH, reducing power, ferric reducing antioxidant power (FRAP), and ABTS radical scavenging activity decreased in this order: 70% methanol, 70% ethanol, EA, CM, n-butanol extracts. NO radical scavenging activity was high in the n-butanol extract (36.73% in 0.8 mg/ml concentration) and low in the 70% ethanol extract (24.96% in 0.8 mg/ml concentration). $OH^-$ radical scavenging activity of n-butanol extract (40.42% in 0.8 mg/ml concentration) was higher than the other extracts, but EA extract was the lowest (13.39%) at the same concentration. The results show that each extract contained various antioxidative substances and it seemed that different influence at each antioxidative activity.

• Korean Journal of Organic Agriculture
• /
• v.16 no.4
• /
• pp.455-469
• /
• 2008

A research was conducted to investigate the effect of treatment of woody charred materials such as wood vinegar, charcoal, and mixture of wood vinegar and charcoal on the growth and components of tomato and chinese cabbage. The effect of treatment of woody charred materials on the growth and components of tomato the research findings are as follows. The total number of soil microorganisms increased in the groups treated with woody charred materials compared with the control. The fruit number, fruit length, fruit diameter, fruit weight, hardness and sugar contents of tomato did not show significant difference in all plots treated with woody charred materials, but the plot treated with wood vinegar + charcoal showed relatively higher values in general. Vitamin C contents were shown higher in the plots treated with woody charred materials than the control, and among the treatment plots. The effect of treatment of woody charred materials on the growth and components of chinese cabbage the research findings are as follows. The soil chemical components did not change before and after the treatment of woody charred materials. The total number of soil microorganisms increased in the treatment plots compared with the control, The leaf length, leaf width, inner leaf number, plant weight, and head weight of Chinese cabbage treated with woody charred materials were in general larger than the control. Among the treatment plots, the plot treated with wood vinegar + charcoal showed slightly higher values. The contents of minerals in chinese cabbage such as Ca, Fe, K, Mg and Mn were higher in the treatment plots than the control.

• Korean Journal of Environmental Agriculture
• /
• v.31 no.4
• /
• pp.368-374
• /
• 2012

BACKGROUND: The urgency of feeding the world's growing population while combating soil pollution, salinization and desertification requires suitable biotechnology not only to improve crop productivity but also to improve soil health through interactions of soil nutrient and soil microorganism. Interest in the utilization of microbial fertilizer has increased. A principle of nature farming is to produce abundant and healthy crops without using chemical fertilizer and pesticides, and without interrupting the natural ecosystem. Beneficial microorganisms may provide supplemental nutrients in the soil, promote crop growth, and enhance plant resistance against pathogenic microorganisms. We mixed beneficial microorganisms such as Bacillus sp. Han-5 with anti-fungal activities, Trichoderma harziaum, Trichoderma longibrachiatum with organic material degrading activity, Actinomycetes bovis with antibiotic production and Pseudomonas sp. with nitrogen fixation. This study was carried out to investigate the mixtures on the soil microflora and soil chemical properties and the effect on the growth of lettuce and cucumber under greenhouse conditions. METHODS AND RESULTS: The microbial mixtures were used with each of organic fertilizer, swine manure and organic+swine manure and compared in regard to changes in soil chemical properties, soil microflora properties and crop growth. At 50 days after the treatment of microorganism mixtures, the pH improved from 5.8 to 6.3, and the EC, $NO_3$-Na and K decreased by 52.4%, 60.5% and 29.3%, respectively. The available $P_2O_5$ and $SiO_2$ increased by 25.9% and 21.2%, respectively. Otherwise, the population density of fluorescent Pseudomonas sp. was accelerated and the growth of vegetables increased. Moreover, the population density of E. coli and Fusarium sp., decreased remarkably. The ratio of bacteria to fungi (B/F) and the ratio of Actinomycetes bovis to fungi (A/F) increased 2.3 (from 272.2 to 624.4) and 1.7 times (from 38.3 to 64), respectively. Furthermore, the growth and yield of cucumber and lettuce significantly increased by the treatment of microorganism mixtures. CONCLUSION(S): These results suggest that the treatment of microorganism mixtures improved the chemical properties and the microflora of soil and the crop growth. Therefore, it is concluded that the microorganism mixtures could be good alternative soil amendments to restore soil nutrients and soil microflora.

• Korean journal of applied entomology
• /
• v.7
• /
• pp.39-51
• /
• 1969

The study involved determination of resistance levels of spider mites ta argano-phosphates using topical application and slide dip techniques; laboratory serening tests of alternative acaricides using an O/P resistant strain and a field trial of the screened materials. 1. Strains of Tetranychus were from Timaru(TR), Havelock Narth (HNR), Lincaln (LN). Germany (GR, GN). Comparisons of the resistant strains and normal strains at the LD50 and LC50 levels were as follows : (a) Using the topical application tochnique; with Parathian. resistant levels of the GR. TR and HNR strains of T. urticae were respeativuly, 1035. 484 and 452 times as resistant' as the LN strain. (b) Using the slide dip technique; with Phosdrin, resistant of GR, TR and HNR strains of T. urticae were 635, 274 and 266 times greater respeativuly, than the GN strain. 2. The laboratory sereaning tests were carried out far their contact plus stomach and residual effect to assess the toxicities of eleven alternative materials which would be used far control of O/P resistant strain of T. urticae. The acaricide groups represented were 3 organo-chlorines (Spidex, Kelthane and C 8514), 2 nitrophenyls (UC 19786 and Morocide), 2 cyclic carbonates(Eradex and Morestan). I carbamate (UC2004 7A), 1 mixture of carbamate and orano-chlorine and 2 other chemicals (C 8677 and M2527). From all acaricide tested. Kelthane and Morocide were the most effective, folowed by Spidex and M2527. Morestan, C8514. C8677 and RS 143 were intermediate, but Eradex, UC 19786 and UC 20046A were poor. 3, The number of sapmles required for estimation of the population in the field evaluation of acaricidal effects was one giving the highest practical precision. It was decided, after preliminary sampling trials. to use samples of 30 leaves per replicate which gave a $5.7\%$ standard error. 4. In the field trials, Morocide applied at the $0.05\%\;and\; 0.04\%$ a. i. conc. to black currant trees gave excellent control of O/P resistant population of T. urticae for about 12 days, but Morocide 0.025 and Kel thane $0.02\%$ a. i. cone. gave efficient control for about 6 days. In other words. first applications of Kel thane ane Moroeide gave very high degrees of control of O/P resistant population of the two-spotted spider mite. However, the results indicate that secondary application would sometimes be necessary. There was no foliage damage of black Currants and strawberries by either acaricides at the concentrations used. Acknowledgment ... The authors are grateful to: Dr. R. P. pottinger, Senior Lecturer in Agricultural Zoology. Lincoln college. New Zealand. for his helpful assistance in aiding with the organization of thd field work. Department of agriculture officers for mite colonies. Mr. D. A. Slade, Technical Advisor. Fruitgrowers' Federation (now at Massey University) for his assistance and provision of mites for testing. Mr T. McRae of Timaru for permission to use his crops for field tests. The following chemical companies and I or their New Zealand agents for so readily supplying samples of acarides; Ivan Watkins-Dow Limited. Fruitgrowers Chemical Company Limited. Henry H. York & company (New Zealand). Shell Oil (New Zealand) Limited.

• 보존과학연구
• /
• s.33
• /
• pp.5-17
• /
• 2012

An Ola leaf manuscript, which consists of words carved on an Ola leaf and is filled up with a mixture of herbal oils made from Vateria acuminata Hyne and charcoal, is one of the native writing media in Sri Lanka. According to historical records, Ola leaf manuscripts had been used from the 1st to the 18th century A.D. From the recent findings that Ola leaf manuscripts have been preserved well for the past 400 years and are in good state of preservation, it is supposed that herbal oils preserve Ola leaves against environmental and biological factors such as fungi and insects. To evaluate the anti-biological susceptibility of the herbal oils, the molds isolated from wooden printing blocks in Janggyeong Panjeon of Haeinsa Temple in South Korea and bacteria and fungi isolated from Ola leaves were cultured. After spreading the microorganisms suspension on an agar plate, a disk paper containing a certain volume of herbal oil was placed on the agar plate. It was found in the experiment that herbal oil exhibits a clear zone, which is optically clear and inhibits the growth of microorganisms, against some molds and bacteria. The study results indicate that the herbal oil from the plant Vateria acuminata Hyne has the anti-bacterial and anti-fungal properties.

• Journal of Life Science
• /
• v.21 no.4
• /
• pp.554-560
• /
• 2011

This study was performed to investigate the effects of microbial augmentation on the biological treatment of paper mill wastewater. Three bacteria (KN11, KN13, KN27) capable of degrading aromatic compounds and a bacterial strain (GT21) producing an extracellular cellulase were isolated from soil and wastewater by selective enrichment culture. Through morphological, physiological, and biochemical taxonomies, isolated strains of KN11, KN13, KN27, and GT21 were identified as Acinetobacter sp., Neisseria sp., Bacillus sp., and Pseudomonas sp. and named Acinetobacter sp. KN11, Neisseria sp. KN13, Bacillus sp. KN27, and Pseudomonas sp. GT21, respectively. For analysis of non-biodegradable and chemical oxygen demand (COD)-increasing matter in a paper mill wastewater, we utilized GC/MS to detect aromatic compounds and their derivatives containing several substituted functional groups. The microbial augmentation, J30 formulated with the mixture of bacteria including Acinetobacter sp. KN11, Neisseria sp. KN13, Bacillus sp. KN27, and Pseudomonas sp. GT21, was used for the treatment of paper mill wastewater. The optimum temperature and pH for COD removal of the microbial augmentation, J30, were $30^{\circ}C$ and 7.5, respectively. For evaluation of the industrial applicability of the microbial augmentation, J30 in the pilot test, treatment efficiency was examined using paper mill wastewater. The microbial augmentation, J30, showed a COD removal rate of 87%. On the basis of the above results, we designed the wastewater treatment process of the activated sludge system.