• Journal of Biosystems Engineering
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• 제19권1호
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• pp.42-49
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• 1994

Texturization of plant protein means the physical or chemical recomposition method of plant protein damaged during the extracting process of soybean oil. As a stable protein supplement, substituted for meat, needs of texturized products have been increased. Twin screw extruder is a very effective tool for texturization process as a physical method. This research, using defatted soy flour as raw material and twin screw extruder manufactured in domestic, showed that plant protein was texturized successfully on the operating conditions of barrel temperature of $120{\sim}140^{\circ}C$, material feed rate of 30~36kg/hr and water content of 20~25%. It also showed that the shape of die affected the texturization continuity.

• Plant Resources
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• 제6권3호
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• pp.221-226
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• 2003

To improve industrial scale extraction method for extraction of icariin from Epimedium koreanum Nakai, the yields under different extracting conditions such as solvent, temperature, duration and solvent to plant material weight ratio were compared. Regarding extracting solution, highest extracts and icariin yield could be achieved when 10% EtOH was used. In case of plant material to extracting solvent ratio, no significant differences could be observed from 1/10 to 1/50, indicating 1/10 was the most efficient. Extracting temperature significantly affected extracts and icariin yields in that 9$0^{\circ}C$ increased the collected extracts and icariin contents up to 29.6% and 0.76%, respectively, compared to 27.2%, 0.33% at 7$0^{\circ}C$. The yield of extracts was less dependent upon extracting temperature compared to icariin yield. Regarding extraction time, 4 hr and 6 hr resulted in high extracts and icariin yield, respectively. We found extracting Epimedium koreanum Nakai in 10 times volume of 10% EtOH for 4 and 6 hr at 9$0^{\circ}C$ seem to be relatively efficient methods for extracts and icariin, respectively.

• Journal of Microbiology and Biotechnology
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• 제19권6호
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• pp.573-581
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• 2009

The complex enzyme pool secreted by the phytopathogenic fungus Fusarium graminearum in response to glucose or hop cell wall material as sole carbon sources was analyzed. The biochemical characterization of the enzymes present in the supernatant of fungal cultures in the glucose medium revealed only 5 different glycosyl hydrolase activities; by contrast, when analyzing cultures in the cell wall medium, 17 different activities were detected. This dramatic increase reflects the adaptation of the fungus by the synthesis of enzymes targeting all layers of the cell wall. When the enzymes secreted in the presence of plant cell wall were used to hydrolyze pretreated crude plant material, high levels of monosaccharides were measured with yields approaching 50% of total sugars released by an acid hydrolysis process. This report is the first biochemical characterization of numerous cellulases, hemicellulases, and pectinases secreted by F. graminearum and demonstrates the usefulness of the described protein cocktail for efficient enzymatic degradation of plant cell wall.

• 한국전기전자재료학회논문지
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• 제27권2호
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• pp.126-131
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• 2014

In this paper, plant crops used in the region to grow crops for the LED lighting area of a rectangle to fit the light distribution to maximize the efficiency of a rectangular distribution was made of LED lights. After the fabrication of LED lamps, light distribution, and in the area of $1.2m{\times}3m$ was the analysis of Illuminance chart. As a result of examining the performance light distribution for total Emin/Eav is 56%, and the target area of the light distribution compared to normal lamps Emin/Eav is improved by about 17%. In addition, plants grown in the area to minimize the outgoing light distribution can be focused on the cultivation area, according to the distance of the plants and be able to have the best luminous efficacy is judged.

• 한국건축시공학회지
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• 제12권6호
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• pp.648-663
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• 2012

With recent attempts to improve quality and productivity, the prefabrication manufacturing system has been occupying an increasing share of the construction area. To minimize site work, material is more frequently being produced and partially assembled at a plant, and then installed at a site. For this reason, the production process is being divided and the materials are being delivered to the site after passing through multiple plants. With these changes in the production process, the materials delivery plan is becoming an important management point. In particular, as road transportation using trucks has a 71 percent share of the domestic transportation market, selecting the proper transportation path is important when delivering materials and equipment to a site. But the management system at the project design phase to calculate the delivery cost by considering the production process of the pre-fab material and the $CO_2$ emission at the material delivery phase is currently lacking. This study suggests a process design model for assembly production of the pre-fab material and transportation logistics based on carbon emission. The suggested model can be helpful to optimize the location of the intermediate plant. It is expected to be utilized as a basic model at the project plan and design phase when subcontractors make decisions on items such as materials procurement, selecting the production method, and choosing the location of the assembly plant.

• 한국수소및신에너지학회논문집
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• 제16권4호
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• pp.372-378
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• 2005

Ni/YSZ ($Y_2O_3$-stabilized $ZrO_2$) composite powder for a cathode material in high temperature electrolysis(HTE) was synthesized by a mechanical alloying method with Ni and YSZ powder. Microstructure of the composite and cell thickness for HTE reaction has been analyzed with various techniques of XRD, SEM to investigate effects of fabrication conditions. Employing the composite material, furthermore, the unit cell for HTE has been studied to evolve hydrogen from water. XRD patterns showed that the composites after wet mechanical alloying were composed of respective nano-sized crystalline Ni and YSZ. While ethanol as additive for mechanical alloying increased to $20\;{\mu}m$ of average particle size of the composites, alpha-terpineol effectively decreased to sub-micro size of that. This study has been found out the evolution of hydrogen by HTE reaction employing the fabricated cathode material, showing 1.4 ml/min of $H_2$ generation rate as increasing $20\;{\mu}m$ of cathode thickness.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 춘계 학술발표대회
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• pp.125-125
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• 2003

To assist genetic study of the root development in Panax ginseng, which is one of the most important medicinal plant, expressed sequence tags (EST) analysis was carried out. We constructed a cDNA library using the 14-year ginseng root. Partial sequences were obtained from 2,975 clone. The ESTs could be clustered into 1,991 (70.2%) non-redundant groups. Similarity search of the non-redundant ESTs against public non-redundant databases of both protein and DNA indicated that 1,553 groups show similarity to genes of blown function. These ESTs clones were divided into sixteen categories depending upon gene function. The most abundant transcripts were ribonuclease 1 (67) and ribonuclease 2 (65). Our extensive EST analysis of genes expressed in 14-year ginseng root not only contributes to the understanding of the dynamics of genome expression patterns in root organ but also adds data to the reperoire of all genomic genes.

• Journal of Applied Biological Chemistry
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• 제55권2호
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• pp.71-77
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• 2012

Many of biochemical or physiological processes can be regulated by non-coding RNAs as well as coding RNAs in plants, animals and microbes. Recently, many small RNAs including microRNAs (miRNAs) and endogenous small interference RNAs (siRNAs) and long non-coding RNAs have been discovered from ubiquitous organisms including plants. Biotic and abiotic stresses are main causal agents of crop losses all over the world. Much efforts have been performed for understanding the complex mechanism of stress responses. Up to date, many of these researches have been related with the identification and investigation of stress-related proteins, showing limitation to resolve the complex mechanism. Recently, non-coding RNAs as well as coding genes have been gradually interested because of its potential roles in plant stress responses as well as other biophysical aspects. In this review, various potential roles of non-coding RNAs, especially miRNAs and siRNAs, are reviewed in relation with plant biotic and abiotic stresses.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1999년도 하계학술대회 논문집 B
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• pp.699-702
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• 1999

This paper represents the application of a virtual plant simulator and a Ladder Diagram of PLC. A target plant is a material transport car that repeatedly conveys along the rails. The whole process is automatically operated by PLC. The simulation system consists of the POSFA PLC and the virtual plant simulator. We demonstrate that operation of a LD program corresponds to operation of the virtual plant simulator.

• Journal of Plant Biology
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• 제30권3호
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• pp.201-203
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• 1987

Total RNA was isolated from two months old wheat, rice, tobacco and sweet potato. The procedure used was simple and provided pure RNA preparation. Lysis of plant tissue in a buffer with guanidine thiocyanate and CsCl density gradient centrifugation separated RNA from the rest of the cellular components. Subsequent cholroform/1-butanol extraction and ethanol precipitation were necessary to ensure contaminant-free RNA preparation. Storage of the lysed plant tissue in the buffer with guanidine thiocyanate preserved the sample for two months without noticeable RNA degradation.