• Journal of Plant Biotechnology
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• v.42 no.2
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• pp.83-87
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• 2015

Genetic transformation was affected by material of explant, age of callus, and medium of regeneration. Two rice seed cultivars (Ilpum and Baekjinju) and mediums were investigated in this study for enhancing regeneration of transgenic rice expressed AtBI-1 gene encoding the Arabidopsis thaliana Bax inhibitor. Regeneration rate of Ilpum rice transformant in gelrite of 5 and 8 g were 27.4% and 18.0%, respectively. In Baekjinju, regeneration rate of transformant was 5.4% and 4.3% in 5 and 8 g gelrite, respectively. The highest number of transformant plant in this study was regenerated from Ilpum cultivar on MS medium (30.4%) and was applied for the subsequent experiment. The callus regeneration rate of transformant were 40.7% in callus infection of up-side, it was higher regeneration then in the down-side (3.9%). The regeneration rate of callus of 25 days and 35 days were 14.7% and 38.6%, respectively. The most important application of this work is in genetic transformation of rice, particularly for improvement transgenic plant tissue culture protocol with high frequency of plant regeneration.

• The Plant Pathology Journal
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• v.19 no.1
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• pp.51-56
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• 2003

Rehmannia glutinosa is one of the most important medicinal crops in Korea. However, various plant pathogens, including Fusatium spp., cause great damage on R. glutinosa and result in enormous economic losses. This study was conducted to breed Fusarium-resistant plants by using Agrobacterium tumefaciences and AFP (anti-fungal protein) gene. The plant material used was a native accession of R. glutinosa. The PCR analysis was conducted to verify transgenicity. Based on the PCR analysis, nptII band was observed in transgenic plant genome. Southern blot and AFP protein analyses also showed the expression of this gene in transgenic plants. Expression of AFP in transgenic plants offers the possibility of developing resistance to fungal infection.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1995.06b
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• pp.27-53
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• 1995

In plant pathology there is an increasing necessity for improved cytological techniques as basis for the localization of cellular substances within the dynamic fine structure of the host-(plant)-pathogen-interaction. Low temperature (LT) preparation techniques (shock freezing, freeze substitution, LT embedding) are now successfully applied in plant pathology. They are regarded as important tools to stabilize the dynamic plant-pathogen-interaction as it exists under physiological conditions. - The main advantage of LT techniques versus conventional chemical fixation is seen in the maintenance of the hydration shell of molecules and macromolecular structures. This results in an improved fine structural preservation and in a superior retention of the antigenicity of proteins. - A well defined ultrastructure of small, fungal organisms and large biological samples such as plant material and as well as the plant-pathogen (fungus) infection sites are presented. The mesophyll tissue of Arabidopsis thaliana is characterized by homogeneously structured cytoplasm closely attached to the cell wall. From analyses of the compatible interaction between Erysiphe graminis f. sp. hordei on barley (Hordeum vulgare), various steps in the infection sequence can be identified. Infection sites of powdery mildew on primary leaves of barley are analysed with regard to the fine structural preservation of the haustoria. The presentation s focussed on the ultrastructure of the extrahaustorial matrix and the extrahaustorial membrane. - The integration of improved cellular preservation with a molecular analysis of the infected host cell is achieved by the application of secondary probing techniques, i.e. immunocytochemistry. Recent data on the characterization of freeze substituted powdery mildew and urst infected plant tissue by immunogold methodology are described with special emphasis on the localization of THRGP-like (threonine-hydrxyproline-rich glycoprotein) epitopes. Infection sites of powdery mildew on barley, stem rust as well as leaf rust (Puccinia recondita) on primary leaves of wheat were probed with a polyclonal antiserum to maize THRGP. Cross-reactivity with the anti-THRGP antiserum was observed over the extrahaustorial matrix of the both compatible and incompatible plant-pathogen interactions. The highly localized accumulation of THRGP-like epitopes at the extrahaustorial host-pathogen interface suggests the involvement of structural, interfacial proteins during the infection of monocotyledonous plants by obligate, biotrophic fungi.

• Journal of Applied Biological Chemistry
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• v.49 no.4
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• pp.162-164
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• 2006

The roots of Codonopsis lanceolata afforded tangshenoside I(1) and $\beta$-adenosine (2) as $\alpha$-glucosidase inhibitors. Their structures were unambiguously determined by 1D and 2D NMR data including HMQC and HMBC experiments. Compounds 1 and 2 exhibited weak $\alpha$-glucosidase inhibitory activities in vitro with $IC_{50}$ of 1.4 and 9.3 mM, respectively.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.7 no.4
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• pp.35-41
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• 1998

The workplace noise environment is composed of three basic elements : manufacturing (in a generic sense) facilities, office areas, and the community around the facility. Work must be done by all employees , and this involves communication within a variety of locations within the facility ; areas may be extremely noisy, moderately noisy, or quiet, such as an office. At the same time, the facility should not be annoying to the community. In this paper, the workplace environmental noise of office areas in power plant are studied. Turbine generator in power plant generates the noise of 90∼95 dB(A) in the frequency range of 1 kHz, which may cause occupational hearing loss. By abatement method which are made of isolation material and distance damping effect, about 29.5 dB(A) reduction has been obtained in office areas of the Power Plant . But, the workplace environmental noise of office areas in the power plant is not suited to office's purpose.

• Proceedings of the SAREK Conference
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• 2008.11a
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• pp.642-648
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• 2008

In order to analyze the causes of fossil power plant facilities due to a degradation and corrosion, artificial degraded materials composed of the facilities were manufactured. Various experiment were performed based on mechanical test, microstructure observation, hardness test, electrochemical potentiokinetic reactivation test(EPR) and corrosion scale thickness measurement test. The master curves were write out using Larson-Miller parameter to evaluate the degree of degradation with the above diagnosis methods. These data were applied to materials database of fossil power plant diagnosis. Finally expert system on the fossil power plant diagnosis was developed using the master curves and diagnosis algorithms.

• Korean Journal of Soil Science and Fertilizer
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• v.15 no.3
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• pp.199-206
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• 1982

A field experiment was conducted to compare the effects of compost and rice straw application on the growth of soybean, chemical properties and soil microorganism in newly reclaimed silty clay loamy upland soil. Application rates of the arganic materials were 750 Kg, 1,500 Kg and 3,000 Kg/10a in compost, and 340 Kg, 680 Kg and 1,360 Kg/10a in rice straw. The differences of N.P.K. application rates caused by the two different organic materials were balanced by chemical fertilizers of N.P.K. The results can be summarized as follows; 1. The yield of soybean was increased upon compost application, but no significant difference on yield was observed except luxuriant plant growth upon rice straw application. 2. There was highly possitive correlation between concentration of phosphorous, potassium in soybean plant at 71 days after planting and dry weight of grain vs. stem plus bean chaff of harvest plant in compost applied plots, but no correlation was found in rice straw applied plots. 3. In greneral, the soil water contents in organic material applied plots, expecially in plots of rice straw, were higher than in no organic material plots. However, at wilting point, the soil water content in organic material applied plot was lower compare to no organic material plot. 4. The weight of nodules per soybean plant was heavier notwithstanding the fewer number of soil bacteria and fungi in compost application plots than rice straw application plots.

• Journal of Ocean Engineering and Technology
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• v.32 no.4
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• pp.253-260
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• 2018

Recently, the application of non-steel materials in ships and offshore plants is increasing because of the development of various nonlinear materials and the improvement of performance. Especially, hyper-elastic materials, which have a nonlinear stress-strain relationship, are used mainly in marine plant structures or ships where impact relaxation, vibration suppression, and elasticity are required, while elasticity must be maintained, even under high strain conditions. In order to simulate and evaluate the behavior of the hyperelastic material, it is very important to select an appropriate material model according to the strain of the material. This study focused on the selection of material models for hyperelastic materials, such as rubber used in the marine and offshore fields. Tension and compression tests and finite element simulations were conducted to compare the accuracy of the nonlinear material models due to variations in the stretch ratio of hyper-elastic material. Material coefficients of nonlinear material models are determined based on the curve fitting of experimental data. The results of this study can be used to improve the reliability of nonlinear material models according to stretch ratio variation.

• 한국전산유체공학회:학술대회논문집
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• 2008.03b
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• pp.362-368
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• 2008

The purpose of this is providing employees who take charge of operation and maintenance at power plant with education contents that can be used for self-studying and on the job training through their computers. We developed the education contents for making actually application possible using this piping and instrument diagram(P&ID), operation and maintenance procedure, unit specification and material of 500MW thermal power plant those include unit equipment 3-dimension animation, character and narration performance considering making teaching plan, flexibility, extension, reuse, maintenance and focusing on user. Specially, we developed the flash type education contents about power plant operation based on the plant 3-dimension animation and the spot real picture concerned about new generation trend for power plant incoming employees actual knowledge. in addition, this contents apparently contributed to improve the level of employees technical power as distributed to employees.

• Korean Journal of Plant Taxonomy
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• v.49 no.4
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• pp.275-281
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• 2019

One key step in the plant restoration process is the collection of seeds from the field. For the selection of source populations of target plant species for translocation purposes (reintroduction or reinforcements), several approaches are possible. A practical method involves the use of data from reciprocal transplant studies. If no direct data are available, knowledge of population genetics and the phylogeography of the target species can serve as an alternative. In this short review, we briefly propose guidelines for those collecting seeds for plant species restoration based on population genetics theory, focusing on two main questions: Where does the plant material come from and how are sources designated, and how are seeds efficiently collected from local populations? While genetic data on a larger scale (phylogeography and population genetics) are needed to form a reply to the first question, similar data on a smaller scale (fine-scale genetic structures within populations) are necessary to shed light on the second issue.