• Applied Biological Chemistry
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• 제48권3호
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• pp.201-206
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• 2005

본 연구는 한약재박의 효율적 처리를 위한 실험의 일환으로 한약재박으로부터 유용미생물을 분리하고 이들 미생물로부터 작물생육촉진미생물을 선발하여 한약재박에 적용한 한약재박 미생물제제를 만들어 친환경농업에 이용하고자 실시하였다. 한약재박은 유기물 함량이 93.0%로 높고 조단백질 함량이 11.3%, 조지방 함량이 5.1%, NDF 함량이 49.7% 및 ADF 함량이 33.8%로 나타나 미생물제제의 원료로서도 이용 가치가 높았다. 한약재박으로부터 약 35종의 미생물이 검출되었고, 이들 중 13종이 한약재박 이용성이 높았으며, 작물생육촉진미생물 BL-333 균주를 선발하였다. 선발균주 BL-333을 Bergey's Manual of Systematic Bacteriology 및 16S rDNA 염기서열(589 bp)로 상동성을 조사한 결과 Paenibacillus marcerans로 추정되었다. P. marcerans BL-333 균주는 각종 진균에 대해 대부분 높은 항진균 활성을 나타내었으며, 특히 Fusarium 속과 Collectotrichum 속 등의 진균에 대해 높은 항진균 활성을 나타내었다. P. marcerans BL-333 균주와 한약재박 분쇄물 등으로 제조한 미생물제제는 10종의 작물의 생육조사에서 무처리구보다 $3{\sim}24%$ 증수되는 효과를 나타내었으며, 특히 상추, 무, 배추 및 오이 등에서 생육촉진효과가 우수하였다. 따라서 한약재박 및 한약재박으로부터 얻은 미생물은 퇴비나 미생물제제를 위해 효과적인 재료로서의 활용 가치가 있다고 본다.

• 한국약용작물학회지
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• 제25권1호
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• pp.37-44
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• 2017

Background: The light emitting plasma (LEP) has recently attracted attention as a novel artificial light source for plant growth and functional component enhancement. We investigated the effects of LEP on whitening and antioxidant activities of the plant parts of perilla. Methods and Results: Previously germianted seeds of perilla were cultivated under different light conditions (fluoresce lamp, LED red, blue, white, green, and LEP) in a culture room for 2 months. Parts of perilla were harvested and extracted in 70% EtOH. The extracts were used to detect total phenolic contents, total flavonoid contents, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), reducing power and tyrosinase inhibition activity as indicators of biological activity. Biological activity was highest in seedlings grown under LEP. The total phenolic content was highest in the stems and the total flavonoid content was highest in the roots of perilla exposed to LEP. The DPPH and ABTS radical activity in all the parts of perilla exposed to LEP were higher by approximately three-fold compared to that in the control (fluoresce lamp). The reducing power values of perilla significantly increased after treatment with LEP. In addition, all the extract of perilla plants exposed to LEP promoted the tyrosinase inhibitory activity. These results suggest that LEP can be an important artificial light source for enhancement of biological activity. Conclusions: LEP could promote whitening and antioxidant activity of perilla.

• Toxicological Research
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• 제22권3호
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• pp.221-228
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• 2006

Bonogen shampoo is composed of several plant extracts which are known to be used in oriental medicine. This study was carried out to investigate the effects of Bonogen shampoo on hair growth in an alopecia model of C57BL/6 mice. There were eight male and female experimental groups including distilled water(DW: negative control), a commercial shampoo[M], 3% minoxidil (MXD) and Bonogen shampoo(BNG). Dorsal skin hair of six-week-old mice was trimmed with an electric clipper carefully not to damage the skin. The next day, mice without skin scratch were selected, randomized and separated in 10 mice per group. The test compounds were topically treated with 0.15 ml per mouse or dorsal skin for 21 days daily and then washed thoroughly with DW. The hair regrowth was determined photographically at 0, 4, 7, 10, 15, 18, and 21 days and histologically at day 21. No clinical signs were observed in all mice. Although body weight was slightly increased in 3% MXD group than other groups, it was not significant. Hair regrowth began to be promoted after 14 days and appeared a distinct regrowth pattern in all animals by topical treatment of test compounds at 18 days. In particular, the topical treatment of bonogen shampoo or 3% MXD for 21 days to dorsal skin accelerated hair regrowth faster than DW or M shampoo. At 21 days, the hair regrowth promotion speed was in order of 3% MXD>BNG>M>DW. The bonogen shampoo or 3% MXD also promoted hair follicle elongation compared to the negative control. These results suggest that bonogen shampoo has hair growth promoting activities and may be useful for treatment of bald or alopecia.

• 생물환경조절학회지
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• 제14권4호
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• pp.275-279
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• 2005

백침계 오이는 측지착과형으로 주지착과형인 국내용 오이와 차이가 있어 백침계 오이의 수량을 증가시키기 위해서는 측지발생을 높여야 가능하다. 그러나 백질계 오이의 재배시기는 대부분 동절기로 저온 및 일조부족 등의 불량한 환경조건 때문에 측지발생이 떨어진다. 본 연구는 생장조정제를 이용하여 백침계 오이의 측지발생을 촉진하기 위하여 BA의 농도 및 살포시기를 구명하고자 하였다 BA $30mg{\cdot}L^{-1}$을 살포한 것은 농도 장해증상이 억제작형에서는 나타났으나, 반촉성작형에서는 경미하게 나타났다. 측지발생은 BA $10mg{\cdot}L^{-1}$ 살포하는 것이 BA $30mg{\cdot}L^{-1}$ 살포하는 것 보다 많았고, 처리시기는 본엽 10매, 15매 전개되었을 메 살포한 것이 본엽 5매 전개되었을 때 살포한 것보다 많았다.

• 한국습지학회지
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• 제16권1호
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• pp.11-18
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• 2014

증가된 이산화탄소 농도와 온도상승에 따른 멸종위기 수생식물 독미나리의 생태학적 반응을 알아보기 위해 대조구, 온도상승구, 그리고 $CO_2$+온도상승구에서 생육시킨 결과를 비교하였다. 독미나리의 지상부 길이는 대조구, 온도상승구 그리고 $CO_2$+온도상승구간에 차이가 없었지만, 분얼 수는 대조구, 온도상승구, $CO_2$+온도상승구 순으로 증가했다. 복산형화서의 수는 대조구와 온도상승구에서 같았고, $CO_2$+온도상승구에서 감소했다. 소산형화서의 수는 대조구와 온도상승구, $CO_2$+온도상승구간에 차이가 없었다. 종자형성률은 온도상승구와 $CO_2$+온도상승구가 대조구보다 감소했고, 온도상승구와 $CO_2$+온도상승구간에 차이가 없었다. 이상으로 보면, $CO_2$농도와 온도상승은 길이생장에 영향을 주지 않은 반면, 분얼 수는 증가시키고 종자생산은 감소시킨다. 그러므로 독미나리는 지구 온난화 조건이 되면 종자생산보다 분얼을 늘려 무성번식 경향이 증가될 것이며, 이러한 개체군 생장을 연구하는 것은 멸종위기종 연구에 중요한 자료로 활용될 것이다.

• Journal of Ginseng Research
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• 제39권3호
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• pp.213-220
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• 2015

Background: Korean ginseng (Panax ginseng Meyer) is a perennial herb prone to various root diseases, with Phytophthora cactorum being considered one of the most dreaded pathogens. P. cactorum causes foliar blight and root rot. Although chemical pesticides are available for disease control, attention has been shifted to viable, eco-friendly, and cost-effective biological means such as plant growth-promoting rhizobacteria (PGPR) for control of diseases. Methods: Native Bacillus amyloliquefaciens strain HK34 was isolated from wild ginseng and assessed as a biological control agent for ginseng. Leaves from plants treated with HK34 were analyzed for induced systemic resistance (ISR) against P. cactorum in square plate assay. Treated plants were verified for differential expression of defense-related marker genes using quantitative reverse transcription polymerase chain reaction. Results: A total of 78 native rhizosphere bacilli from wild P. ginseng were isolated. One of the root-associated bacteria identified as B. amyloliquefaciens strain HK34 effectively induced resistance against P. cactorum when applied as soil drench once (99.1% disease control) and as a priming treatment two times in the early stages (83.9% disease control). A similar result was observed in the leaf samples of plants under field conditions, where the percentage of disease control was 85.6%. Significant upregulation of the genes PgPR10, PgPR5, and PgCAT in the leaves of plants treated with HK34 was observed against P. cactorum compared with untreated controls and only pathogen-treated plants. Conclusion: The results of this study indicate HK34 as a potential biocontrol agent eliciting ISR in ginseng against P. cactorum.

• 한국토양비료학회지
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• 제32권1호
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• pp.57-61
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• 1999

본 연구는 밀양 23호와 내풍벼 볍씨를 Brassino-steroids에 24시간 침종 후 인공산성비에 7일간 발아시킨 후 생육, 발아율, 유기산 함량 등 몇 가지 조사를 수행하였던 바 얻어진 결과를 요약하면 인공 산성비의 조성은 sulfate가 $427.9mg\;kg^{-1}$으로 가장 많았고 그 다음이 nitrate로 $130.6mg\;kg^{-1}$이었다. 초장은 밀양 23호에서 인공 산성비 처리구보다 식물생장조절제 처리구가 길었으나 내풍벼에서는 식물생장조절제의 종류와는 무관하게 감소하였고 근장은 밀양 23호에서 인공 산성비 처리구보다 식물생장조절제 처리구에서 높은 뿌리의 신장 효과를 보였다. 발아율은 밀양 23호에서는 인공산성비 단일 처리구가 84.3%인 것보다 모든 생장조절제 처리에서 88.3%의 발아율을 보였으며 유기산 함량은 밀양 23호에서는 인공 산성비 처리구에서 succinic acid와 acetic acid가 각각 $878,419{\mu}g$으로 가장 높은 농도를 보였고 hBR처리에서는 농도가 낮은 $10^{-3}mg\;kg^{-1}$에서 succinic acid와 acetic acid가 가장 높은 함량을 나타내었으나 epiBR처리에서는 acetic acid는 농도가 높을 때, succinic acid는 농도가 낮을 때 함량이 증가하였다.

• 한국미생물·생명공학회지
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• 제36권4호
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• pp.326-335
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• 2008

Bacillus subtilis AH18균주는 auxin, siderophore 그리고 cellulase를 동시에 생산하는 PGPR 균주이자 생물방제균주로 항진균성 siderophore의 특성을 확인한 결과 catechol type의 siderophore로 확인하였다. $Siderophore_{AH18}$ 의 정제는 Amberlite XAD-2, sephadex LH-20 column chromatography 그리고 HPLC를 통해 정제 및 정제여부를 확인하였으며, GC-MS, $^1H$-NMR, 그리고 $^{13}C$-NMR을 통해 구조 및 분자량을 확인하였다. 그 결과 B. subtilis AH18이 생산하는 siderophore은 분자량 883의 bacillibactin임을 확인하였으며, 포자발아억제활성을 나타냄을 확인하였다. B. subtilis AH18은 P. capsici에 의한 고추역병을 효과적으로 방제하였으며(방제력 55%), 이는 bacillibactin에 의한 효과가 포함되리라 추측된다.

• 한국미생물·생명공학회지
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• 제35권4호
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• pp.357-363
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• 2007

249 fungal strains were isolated from the roots of 26 plants, and the production of GAs was spectrophotometric ally examined. As a result 76 fungal strains were shown to produce GAs. Bioassay of culture broth from seventy six fungal strains producing GAs was carried out with waito-c rice, that is dwarf rice. The seventy six fungi with GAs-producing activity were incubated for seven days in 40 mL of Czapek's liquid medium at $30^{\circ}C$ and 180 rpm, and the culture broth of fungi were treated on the 2-leaf rice sprout. Fifteen of these showed plant growth promoting activity and the amount of each GAs in the medium was measured by Gas chromatographymass spectrometer (GC-MS). Nine of these fungi were also identified by genetic analysis of the nucleotide sequences in the internal transcribed spacer region of the ribosomal DNA.

• 한국토양비료학회지
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• 제49권2호
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• pp.144-156
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• 2016

Soil is a dynamic biological system, in which it is difficult to determine the composition of microbial communities. Knowledge of microbial diversity and function in soils are limited because of the taxonomic and methodological limitations associated with studying the organisms. In this review, approaches to measure microbial diversity in soil were discussed. Research on soil microbes can be categorized as structural diversity, functional diversity and genetic diversity studies, and these include cultivation based and cultivation independent methods. Cultivation independent technique to evaluate soil structural diversity include different techniques such as Phospholipid Fatty Acids (PLFA) and Fatty Acid Methyl Ester (FAME) analysis. Carbon source utilization pattern of soil microorganisms by Community Level Physiological Profiling (CLPP), catabolic responses by Substrate Induced Respiration technique (SIR) and soil microbial enzyme activities are discussed. Genetic diversity of soil microorganisms using molecular techniques such as 16S rDNA analysis Denaturing Gradient Gel Electrophoresis (DGGE) / Temperature Gradient Gel Electrophoresis (TGGE), Terminal Restriction Fragment Length Polymorphism (T-RFLP), Single Strand Conformation Polymorphism (SSCP), Restriction Fragment Length Polymorphism (RFLP) / Amplified Ribosomal DNA Restriction Analysis (ARDRA) and Ribosomal Intergenic Spacer Analysis (RISA) are also discussed. The chapter ends with a final conclusion on the advantages and disadvantages of different techniques and advances in molecular techniques to study the soil microbial diversity.