• Title/Summary/Keyword: plant debris

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Recovery of Plant Cell and Its Debris by pH Control (pH 조절에 의한 식물세포 및 세포조각 회수)

  • 김진현
    • KSBB Journal
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    • v.15 no.4
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    • pp.405-407
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    • 2000
  • A novel recovery method was developed to obtain the plant cell and its debris from Taxus chinensis cell cultures By pH control of plant cell cultures plant cell and debris was precipitated. The best pH is between 1.8 and 2.2 to obtain the precipitate of the plant cell and debris. Also paclitaxel is stable in this acidic conditions. This method is very simple an efficient to recover the plant cell and debris from plant cell cultures.

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Investigation of Floating Debris Characteristics Drained from 4 Big River on a Flooding (홍수시 4대강에서 유입되는 부유폐기물 성상 조사)

  • Yu J. S.;Yoon B. S.;Rho J. H.;Yoon S. H.
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.5 no.3
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    • pp.45-53
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    • 2002
  • This investigation is performed to prepare reducing method drained floating debris from the river This paper is present an investigation result of the marine debris characteristics that drained from korea 4 big river(han river, kum river, youngsan river, nakdong river) during July and August. A mount of floating debris different with rainfall. Short heavy rain like as 150mm/day floating debris drained lower, almost floating debris drained when a flooding cause by continuance heavy rain. Floating debris draining is not continuance, but concentrated on a flooding. All debris is do not drained ocean, a lot of debris accumulated riverside. Floating debris is drained with plant and configuration is similar with other river. But, the component ratio is different, so that, to Prepare removing method for floating debris consider that effect of plant debris.

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Effects of Temperature and Moisture on the Survival of Colletotrichum acutatum, the Causal Agent of Pepper Anthracnose in Soil and Pepper Fruit Debris

  • Kang, Beum-Kwan;Kim, Joo-Hyeong;Lee, Kyeong-Hee;Lim, Sang-Cheol;Ji, Jae-Jun;Lee, Jong-Won;Kim, Heung-Tae
    • The Plant Pathology Journal
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    • v.25 no.2
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    • pp.128-135
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    • 2009
  • The survival of Colletotrichum acutatum was investigated in soil, infected fruits, and infected fruit debris incorporated into soil at several temperatures with different soil moisture levels. Samples were examined at 2-week intervals for 18 weeks to determine the survival of the pathogen based on the number of colony forming unit (CFU) of C. acutatum recovered on a semi-selective medium. C. acutatum conidia survived in both sterile and non-sterile soil at 4 and $10^{\circ}C$ for 18 weeks. If infected pepper fruits were completely dried, C. acutatum survived for 18 weeks at temperature from 4 to $20^{\circ}C$. Soil temperature and moisture affected the survival of C. acutatum in infected fruit debris incorporated into soil after air-drying. The effect of soil moisture on survival was weaker at low temperatures than at high temperatures. For up to 16 weeks, conidia were recovered from fruit debris in soil that had been kept at 4 to $20^{\circ}C$ and below 6% soil moisture. Conidia were recovered from fields until approximately 6 months after pepper fruits were harvested. Using PCR with species-specific primers and a pathogenicity test, we identified conidia recovered from soil and infected fruit from both the laboratory and field as C. acutatum and as the primary inoculum causing pepper anthracnose.

Numerical Evaluation of Debris Transport During LOCA Blow-Down Phase of OPR1000 Nuclear Power Plant (CFD 를 이용한 OPR1000 원자력발전소 파단방출이동에 대한 수치해석적 평가)

  • Choi, Kyung-Sik;Park, Jong-Pil;Jeong, Ji-Hwan;Kim, Won-Tae
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.35 no.3
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    • pp.255-262
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    • 2011
  • In a loss-of-coolantaccident, considerable debris may be generated and transported to the recirculation sump. The accumulation of debris will reduce the netpositivesuctionhead and threaten the safety of thenuclear power plant. Both NEI 04-07 and USNRC SER suggesteda CFD methodology. However, additional investigation is needed to consider the unique characteristics of nuclear power plants. The transport of the generated debris is strongly influenced by the break location and the plant characteristics, including the configuration.In this paper, a CFD methodology for blow-down transport evaluation is proposed and applied to an OPR1000 nuclear power plant. The results show that the percentage of small debris transported to the upper containment is 32%, which is 7% larger than the valuegiven in the NEI 04-07 baseline analysis. This result may be used as a point of reference in future analytical studies.

Overwinter and Survival of Colletotrichum gloeosporioides and Glomerella cingulata in Soil and Plant Debris of Strawberry (딸기 병걸린 식물잔재물과 토양에서 탄저병균 Colletotrichum gloeosporioides와 Glomerella cingulata의 월동과 생존)

  • Nam, Myeong-Hyeon;Song, Jung-Young;Kim, Hong-Gi
    • Research in Plant Disease
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    • v.10 no.3
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    • pp.198-202
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    • 2004
  • The overwinter and survival of Colletotrichum gloeosporioides and Glomerella cingulata in strawberry tissues under field conditions was investigated in 2001 and 2002. The rates of overwinter survival in plant petiole, runner and crown were 68.7,14.3, and 26.7%, respectively. But, under field condition, viable conidia of overwinter were not detected at the surface, 3, 5, and 10 cm depths of soil. To investigate the survival ability in soil and plant debris, C. gloeosporioides and G. cingulata isolates from strawberry were examined in laboratory and field. The viability of conidia was rapidly declined in all the treatments, with a 40% reduction in population within 10 days. In soil, the survival ability of G. cingulata conidia was similiar to that of C. gloeosporioides. The survival rate of conidia was highest under cool and dry soil conditions, and was decreased by increasing both soil temperature and moisture content. Similar results were also obtained under the condition that infected petioles were buried in soil. Results suggested that conidia as well as plant debris might be a main primary inoculum source of strawberry anthracnose.

A Study on the Two Phase Flow in the Floor of Containment Building after a Loss of Coolant Accident (냉각재 상실사고 후 격납건물내의 이상유동 연구)

  • Bae, Jin-Hyo;Park, Man Heung;Koh, Chul-Kyun;Lee, Jae-Heon
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.23 no.10
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    • pp.1274-1284
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    • 1999
  • The Regulatory Guide 1.82 recommends an analysis of hydraulic performance for sump of ECCS (Emergency Core Cooing System) when LOCA(Loss of Coolant Accident) occurs in a nuclear power plant. The present study deals with 3-dimensional, unsteady, turbulent and two-phase flow simulation to examine the behavior of mixture of reactor coolant and debris near the floor of containment building in conjunction with appropriate assumptions. The dispersed solid model has been adjusted to the interfacial momentum transfer between reactor coolant and debris. According to the results, the counterclockwiserecirculation zone had been formed in the region between sump and connection aisle about 376 second after LOCA occurs. The debris thickness accumulated on a sump screen periodically increases or decreases up to 2000 second, afterwards its peak decreases.

Vibration Phenomena between Condensate Vertical Pump and Condenser Debris system in Power Plant (발전소 복수기 볼세정계통에 의한 입형펌프 진동과 대책)

  • Kim, Yeon-Whan;Goo, Jae-Rayng;Bae, Yong-Chae;Lee, Hyun;Lee, Young-Shin
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2001.11b
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    • pp.1020-1025
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    • 2001
  • Vertical canned pumps are usually applied in the power plant to transport fluids that the available suction pressure is low at the condensate system and the circulating-water system. The top of the motor driver is 5.5m above the base and the barrel and drop column extend even further below the ground. While this size and configuration may produce an efficient pumping system, it also introduces several dynamic problems which must be considered in order to obtain a reliable operation. The main problem is that the vibrational mode of the long flexible cantilever structure above the ground exist near the operational characteristics of the condensate debris system. This system's trouble has been showed at 2,086 hp condensate water loading pumps in a nuclear power plant.

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Nonlinear Impact Analysis for Eco-Pillar Debris Barrier with Hollow Cross-Section (중공트랙단면 에코필라 사방댐의 비선형 충돌해석)

  • Kim, Hyun-Gi;Kim, Bum-Joon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.7
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    • pp.430-439
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    • 2019
  • In this study, a nonlinear impact analysis was performed to evaluate the safety and damage of an eco-pillar debris barrier with a hollow cross-section, which was proposed to improve constructability and economic efficiency. The construction of concrete eco-pillar debris barriers has increased recently. However, there are no design standards concerning debris barriers in Korea, and it is difficult to find a study on performance evaluations in extreme environments. Thus, an analysis of an eco-pillar debris barrier was done using the rock impact speed, which was estimated from the debris flow velocity. The diameters of rocks were determined by ETAG 27. The impact position, angles, and rock diameter were considered as variables. A concrete nonlinear material model was applied, and the estimation of damage was done by ABAQUS software. As a result, the damage ratio was found to be less than 1.0 at rock diameters of 0.3 m and 0.5 m, but it was 1.39 when the diameter was 0.7 m. This study could be used as basic data on impact force in the design of the cross section of an eco-pillar debris barrier.

The Allelopathic Effects of Lantana camara on Seed Germination and Growth of Selected Bioassay Species

  • Senarathne, S.H.S.;Fernando, R.D.V.;Sangakkara, U.R.
    • Korean Journal of Weed Science
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    • v.31 no.3
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    • pp.271-278
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    • 2011
  • The allelopathic effects of Lantana camara L. (Family:Verbenaceae) on germination and seedling establishment of some agricultural crops and weed species have been identified. Aqueous extracts of dry leaves and contaminated soil where L. camara is grown were used to verify allelopathic effect on seed germination of five bioassay species; Raphanus sativas, Capsicum annum, Lycopersicum esculantem, Crotalaria juncia and Chromoleana odorata. Fifty seeds from each bioassay species were placed in a petri dish containing leaf extracts or contaminated soil, and seed germination were examined after 3 days. The plant house experiments were carried out to evaluate the impact of L. camara contaminated soil and leaf debris using L. esculantem as the indicator plant. Seed germination of L. esculentem, C. junica and Capsicum annum was significantly inhibited by L. camara contaminated soil. However, the degree of inhibition varied among the bioassay species. The aqueous extract of dry leaves of L. camara was highly phytotoxic and it significantly reduced seed germination of all bioassay species. There was a decline in plant height, leaf area and shoot dry weight of tomato only in early growth stages when grown in L. camara contaminated soils. However, incorporation of leaf debris into soil affected the vegetative growth of tomato in early stages when the leaf debris concentration was increased. Growth recovered at the latter part of the life cycle. On the basis of these results it can be concluded that the allelochemicals in L. camara contaminated soils are harmful to the seed germination of crop species. The adverse effect was present only during the early growth stages and it did not suppress the latter part of the plant growth. These responses are attributed to allelopathic effects which need confirmation under field conditions.

Functional Analysis of Genes Specifically Expressed during Aerial Hyphae Collapse as a Potential Signal for Perithecium Formation Induction in Fusarium graminearum

  • Yun-Seon Choi;Da-Woon Kim;Sung-Hwan Yun
    • The Plant Pathology Journal
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    • v.40 no.1
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    • pp.83-97
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    • 2024
  • Fusarium graminearum, the causal agent of Fusarium head blight (FHB) in cereal crops, employs the production of sexual fruiting bodies (perithecia) on plant debris as a strategy for overwintering and dissemination. In an artificial condition (e.g., carrot agar medium), the F. graminearum Z3643 strain was capable of producing perithecia predominantly in the central region of the fungal culture where aerial hyphae naturally collapsed. To unravel the intricate relationship between natural aerial hyphae collapse and sexual development in this fungus, we focused on 699 genes differentially expressed during aerial hyphae collapse, with 26 selected for further analysis. Targeted gene deletion and quantitative real-time PCR analyses elucidated the functions of specific genes during natural aerial hyphae collapse and perithecium formation. Furthermore, comparative gene expression analyses between natural collapse and artificial removal conditions reveal distinct temporal profiles, with the latter inducing a more rapid and pronounced response, particularly in MAT gene expression. Notably, FGSG_09210 and FGSG_09896 play crucial roles in sexual development and aerial hyphae growth, respectively. Taken together, it is plausible that if aerial hyphae collapse occurs on plant debris, it may serve as a physical cue for inducing perithecium formation in crop fields, representing a survival strategy for F. graminearum during winter. Insights into the molecular mechanisms underlying aerial hyphae collapse provides offer potential strategies for disease control against FHB caused by F. graminearum.