• Journal of Life Science
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• v.20 no.9
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• pp.1314-1323
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• 2010

In this study, we constructed various kinds of binary vectors with the pPZP backbone for co-overexpression, tissue- or development-specific expression and stress-inducible expression, and validated them for ectopic expression of target genes. Using a modified CaMV 35S promoter, a binary vector was generated for co-overexpression of two different genes and was confirmed to be efficient for overexpressing two different target genes at the same time and place. Binary vectors containing At2S3, KNAT1 or LFY promoters were constructed for tissue-specific or development-specific gene expression, and the binary vectors were suited for embryo/young seedling stage-, shoot apical meristem- or leaf primordia-specific expressions. Furthermore, the binary vectors containing RD29A or AtNCED3 promoters were validated as suitable vectors for gene expression induced by abiotic stresses such as high salt, ABA, MV and low temperature. Taken together, the binary vectors constructed in this study would be very useful for analyzing the biological functions of target genes and molecular mechanisms through ectopic expression.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.40 no.1
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• pp.31-37
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• 2016

Co-firing of biomass with coal is a promising combustion technology in a coal-fired power plant. However, it still requires verifications to apply co-firing in an actual boiler. In this study, data from the Thermogravimetric analyzer(TGA) and Drop tube furnace(DTF) were used to obtain the combustion characteristics of biomass when co-firing with coal. The combustion characteristics were verified using experimental results including reactivity from the TGA and Unburned carbon(UBC) data from the DTF. The experiment also analyzed with the variation of the biomass blending ratio and biomass particle size. It was determined that increasing the biomass blending ratio resulted in incomplete chemical reactions due to insufficient oxygen levels because of the rapid initial combustion characteristics of the biomass. Thus, the optimum blending condition of the biomass based on the results of this study was found to be 5 while oxygen enrichment reduced the increase of UBC that occurred during combustion of blended biomass and coal.

• Korean Journal of Ecology and Environment
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• v.49 no.3
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• pp.228-235
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• 2016

Aquatic ecosystems are receiving various harmful effects due to anthropogenic chemical pollutions. To protect wildlife, risk assessments of the chemicals are conducted using reference indexes of toxicity estimated by species-level laboratory tests and/or micro-/mesocosm community-level studies. However, the existing micro-/mesocosm communities are structurally too complicated, and it is also difficult to compare the experimental results directly with those from species-level tests. Here, we developed a procedure of a simple bi-trophic microcosm experiment which contains the common species (a green algae, Pseudokirchneriella subcapitata and a cladoceran, Daphnia magna) for testing chemical toxicities. For the proper operation of bitrophic microcosm experiment, the minimum required concentration of primary producer (P. subcapitata) is $5{\times}10^5cells\;mL^{-1}$. The microcosm system showed higher stability when the initially introduced D. magna population was composed of neonates (<24-h old) than adults and those mixture. This simple microcosm system would be an applicable tool to estimate the disturbing impacts of pollutants on plant-herbivore interactions, and linking the species- and population-/community level risk assessments in the future studies.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.1
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• pp.21-30
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• 1991

In Summer, oxygen-deficient water masses were developed extensively in the closed eutrophic bays such as Chinhae Bay which results in mass mortality of marine organisms and severe decrease the production of the bay every year. Under the circumstances, this study was performed to investigate the oxygen depletion relating to eutrophication, and also to evaluate self-purfication capacity of Buk Bay by dissolved oxygen mass balance in 1988. The mean concentration of total inorganic nitrogen, phosphate phosphorus and chlorophyll-a were $11.06{\mu}g-at/l,\;0.80{\mu}g-at/l\;and\;1.11mg/m^3$ respectively, which were over eutrophication criteria. Oxygen-deficient water mass was formed in July with the minimum concentration of 2.08ml/l(mean) at the bottom of all stations and recovered slowly in August. The decay and reaeration coefficient calculated from dissloved oxygen sag curve were 0.222/day and 0.018ml/l/day, respectively. To maintain above 4ml/l of oxygen to prevent oxygen-deficient water mass, it is recommendable to supply as much as 0.856ml/l/day of dissolved oxygen or should be reduced the same mass loading of BOD from watershed by the construction of wastewater treatment plant.

• Journal of Wetlands Research
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• v.9 no.2
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• pp.21-31
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• 2007

Estuarine wetland, where freshwater meets saltwater, is a transitional ecosystem that is valuable ecologically for a variety of reasons, such as feeding and breeding sites for birds, fish, and wildlife. However, research on the spatial distribution and temporal dynamics of estuarine wetlands in Korea is rare. As a fundamental basis for wetland conservation, this study quantified the wetlands in three major estuaries, and evaluated the temporal dynamics of the wetlands since the 1910s. In particular, this study classified the wetland types into mud flat, sand, and emergent-plant types, and estimated the change of each wetland type, using topographic maps produced in the 1910s, 1970s, and 2000s. The wetlands in both the Han and Youngsan River estuaries have declined since the 1910s, but the rate of wetland decline was relatively low before the 1970s, compared to that since the 1970s. The impact of human activities, such as the Youngsan Watershed Comprehensive Development Project and the construction of estuary barrages, has disrupted the estuary cycles and destroyed huge amounts of wetland in the Youngsan estuary. By contrast, estuarine wetlands have been preserved in the small Gahwa estuary, and provide a variety of habitats for plants and wildlife. A special management strategy for wetlands should be established as soon as possible.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.25 no.4
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• pp.191-199
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• 2013

It is estimated and analyzed that the design wave height and the confidence interval (hereafter CI) according to the return period using the fourteen-year wave data obtained at Pusan New Port. The functions used in the extreme value analysis are the Gumbel function, the Weibull function, and the Kernel function. The CI of the estimated wave heights was predicted using one of the Monte-Carlo simulation methods, the Bootstrap method. The analysis results of the estimated CI of the design wave height indicate that over 150 years of data is necessary in order to satisfy an approximately ${\pm}$10% CI. Also, estimating the number of practically possible data to be around 25~50, the allowable error was found to be approximately ${\pm}$16~22% for Type I PDF and ${\pm}$18~24% for Type III PDF. Whereas, the Kernel distribution method, a typical non-parametric method, shows that the CI of the method is below 40% in comparison with the CI of the other methods and the estimated design wave height is 1.2~1.6 m lower than that of the other methods.

• Korean Journal of Environmental Biology
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• v.27 no.2
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• pp.216-229
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• 2009

The flora of vascular plants in the Geumgang area (Mt. Guryongsan, Mt. Jeonwolsan, Mt. Samasan, Mt. Yongmeorisan and Mt. Hamrasan) was investigated to suggest strategies for conservation. This study was performed from May 2007 to April 2008, with total 17 times. The Korean endemic species, legally protected species and species to threaten domestic ecosystem species to threaten were grasped on this study. The flora consists of 334 taxa, 86 families 246 genera 297 species 34 varieies and 3 forms in total results from 5 sites in this study. Korean endemic species were confirmed 8 taxa. No rare and endangered species were found in this study. The 2 taxa of the fourth grade of specially designated plants announced the Ministry of Environment were investigated and 8 taxa of the third grade were confirmed. In totally, 24 taxa that are specially designated plants announced the Ministry of Environment including the first and the second grade taxa. 25 taxa of alien naturalized plants were confirmed. The degree of destroy of forest vegetation and reduction of plant diversity in the midstream of Geumgang were in proceeding by construction of multifunctional administrative city and cultivation. It is needed to set up strategies for conservation forest vegetation in this study area.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.4
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• pp.119-132
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• 2012

The unmanaged abandoned concrete roads are vulnerable toward the issues on soil and water pollution, which requires flexible managing method such as eco-corridor after the process of ecological restoration. Among various alternations of abandoned concrete-paved roads, ecological restoration technique may be the most suitable method in sites including high quality of natural environment. Therefore, as in Young dong province, GyungBu express highway (227.24~229.04km), which is near to Hwang-gan IC, the survey to measure its effect of soil under the paving and water pollution by abandoned concrete roads was discussed. Then, the restoration method of plantings of landscape trees and hydro-seeding methods of artificial soil media was appraised through consecutive monitoring. The soil adequacy analysis shows lower percentage of heavy metal substance in each depth level compared to standard limit stated by the Ministry of Environment, along with low concerns raised after the analysis on heavy metal content of the spilled water on the concrete roads. Meanwhile, Korean Weigela (Weigela subsessilis L.H. Baily) was found to be withered in small-scale landscape trees planting sites. Among the seeding plants. the family of leguminosae, Silene armeria, Dendranthema boreale, Caryopteris incana and Aster yomena show good establishment results. Overall studies on planting of small and large landscape trees, planting method of container plants, planting method of ground cover plants, and germination and development trend of seeding plants of the experimental restoration site on abandoned concrete roads are revealing specific trends in the way landscape woody plants establishment and growth. Finally, this study suggests further studies and survey on varied plant restoration methods on abandoned concrete-roads for developed design guidelines of their methods.

• Horticultural Science & Technology
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• v.35 no.1
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• pp.98-107
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• 2017

A DNA profile database was constructed to investigate the genetic relatedness of 72 germplasm samples of Pyrus and related cultivars using microsatellite markers. Three P. pyrifolia, four P. commus, and one P. betulifolia cultivars with different morphological traits were screened using 387 pairs of microsatellite primers. A core set of 11 primer pairs was selected to obtain 133 polymorphic amplified fragments meeting three criteria: high polymorphism information contents (PIC), high repeatability, and distinct allele patterns. The number of alleles per locus ranged between 4 and 22. Average PIC was 0.743 (range: 0.557 - 0.879). Cluster analysis using the unweighted pair - group method with arithmetical average (UPGMA) separated the 72 pear cultivars and germplasm samples into four major groups: Chinese, European pears, and a cluster of 55 Asian pears that could be reclassify into two subcluster, I - $1^{st}$ and II - $2^{nd}$, according to pedigree information. Almost all of the cultivars were discriminated by 11 microsatellite marker genotypes. The microsatellite DNA profile database may be utilized as tool to verify distinctness, uniformity, and stability between candidate cultivar, and to verify in the distinctness of existing cultivars.

• Journal of the Institute of Convergence Signal Processing
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• v.8 no.3
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• pp.211-216
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• 2007

The quality of tap water on the whole water-supply system, from a large filtration plant to a private faucet, has to be guaranteed the standards of drinking water. At this point in time, however, the supply process of the tap water has not been monitored and managed scientifically. The piped water, especially the most small-scale reservoirs(underground or overhead type) are always exposed to various contaminations and impurities. Recently monitoring systems of water-quality were spread on some large filtration plants or distributing reservoirs. In particular, the water quality monitoring method using the internet is adopted into some local government whose inhabitants can check up the water quality anytime and anywhere. The construction of this system that has to apply a large scale needs, and has a limitation on the small water-supply system, such as apartments, public facilities and small-scale underground or overhead reservoirs. In this work, we suggest the integration system of individual water-quality sensor modules that have a low price. By using the developed integration system and monitoring program operated on the internet, the system managers of reservoirs can monitor and manage water-quality characteristic values of drinking water in online. Since the proposed system was modularized, the system can be applied easily into various reservoirs with a low cost and regardless of its scale, small or large.