• Journal of Microbiology and Biotechnology
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• v.13 no.2
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• pp.301-304
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• 2003

In this study, hydrogen sulfide ($H_2S$), ammonia ($NH_3$), and benzene, which represent the major odor from a natural leather process plant, were removed using a fluidized bed bioreactor and biofilter including Thiobacillus sp. IW and a MY microbial consortium. The critical removal rate was $12g m^{-3}h^{-1}\;for\;H_2S,\;11g m^{-3}h^{-1}\;for\;NH_3\;and\;28 g m^{-3}h^{-1}$ for benzene by the fluidized bed bioreactor, and $8.5g m^{-3}h^{-1}\;for\;H_2S\;7g m^{-3}h^{-1}\;for\;NH_3,\;and\;25 g m^{-3}h^{-1}$ for benzene in the biofilter. The average removal efficiency of $H_2S$, $NH_3$, and benzene by continuous operation for over 30 days with the fluidized bed bioreactor was $95{\pm}3\%,\;99{\pm}1\%,\;and\;98{\pm}5\%$, respectively, whereas that with the biofilter was $96{\pm}4\%,\;95{\pm}4\%,\;and\;97{\pm}3\%$, respectively. Therefore, the critical removal rate of $H_2S$, $NH_3$, and benzene was higher in the fluidized bed bioreactor, whereas the removal efficiency on the continuous operation was similar in both bioreactors.

• Journal of Plant Biotechnology
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• v.1 no.1
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• pp.1-7
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• 1999

Large-scale cultures of plant cell, tissue, and organ have been achieved by using BTBB. When different sized BTBBs (5 L, 20 L, 100 L, 300 L, and 500 L) were tested for the culture of yew cells (Taxus cuspidata Sieb. et Zucc.), cell growth increment reached to 94.5% in SCV after 24 days of culture with 30% of inoculation cell density. However, there were some variations in the production of taxol and its derivatives among the BTBBs of different size. Approximate 4 ㎎/l of taxol and 84 ㎎/l of total taxanes were obtained by using a 500L BTBB after 6 weeks of culture. With a 20L BTBB, about 20,000 cuttings of virus-free potatoes (cv. Dejima) could be obtained by inoculating 128 explants and maintaining 8 weeks under 16 hr light illumination. The frequency of ex vitro rooting of the cuttings revealed as more than 99% under 30% shade. By incorporating two-stage culture process consisting of multiple bulblet formation in solid medium and bulblet development in liquid medium, mass propagation of lily through bioreactor seemed to be possible. In the case of 'Marcopolo', the growth of mini-bulblets in BTBB was nearly 10 folds faster than that of the solid medium. Time course study revealed that maximum MAR yield of ginseng (Panax ginseng C. A. Meyer) in a 5 L and 20 L BTBB after 8 weeks of culture was 500 g and 2.2 ㎏, respectively. By cutting the MAR once and/or twice during the culture, the yield of root biomass could be increased more than 50% in fresh weight at the time of harvest. With initial inoculum of 500 g of sliced MAR in a 500 L BTBB, 74.8 ㎏ of adventitious root mass was obtained after 8 weeks of culture. The average content of total ginseng saponin obtained from small-scale and/or pilotscale BTBBs was approximately 1% per gram dry weight. Based on our results, we suggest that large-scale cultures of plant cell, tissue, and organ using BTBB system should be quite a feasible approach when compared with conventional method of tissue culture.

• Horticultural Science & Technology
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• v.30 no.4
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• pp.432-436
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• 2012

This study was conducted to determine the optimal aeration rate for mass propagation of ever-bearing strawberry by bioreactor culture. The aeration rate was treated in four levels: 0.1 vvm (air volume/medium volume/min), 0.2 vvm, 0.3 vvm, and 0.4 vvm. In 0.2 vvm conditions, shoot length was the longest at 9.03 cm in bioreactor culture, leave numbers were 40.4 ea and fresh weight was 6,106 mg. Plant growth rate at 0.2 vvm condition was faster than other treatments. In the aeration condition, 0.2 vvm was most effective to increase aerial part growth and to decrease medium consumption. As the culture periods increased, the fresh weight also increased rapidly. After six weeks of cultivation, shoots were emerged with 10.4 ea per plantlet, resulting in developing a complete plant. As a result, the bioreactor culture system for mass propagation of strawberry is required to continuously supply the air by 0.2 vvm speed and cultivate at least for six weeks.

• Journal of Plant Biotechnology
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• v.31 no.2
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• pp.127-132
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• 2004

A suitable bioreactor culture system for shoot proliferation and bulblet formation of Allium victorialis var. platyphyllum Makino was established. Uptake of soluble carbohydrates in different bioreactor culture systems was also analyzed during the entire culture period. Optimal conditions for multiple shoot formation were determined in raft culture (RC) and modified raft culture system (MRC) (13-15 per explant) in which the explants were placed on a net contacting liquid medium. For bulblet formation and enlargement, 93.4％ of shoot clumps formed bulblets at the basal part. Furthermore, they were uniform in size when cultured with ebb & flood system (E&FS). Bulblets harvested from RC and MRC showed vigorous rooting, however, their growth was not uniform. Whereas soluble carbohydrate contents in the bulblets cultured in E&FS were low, starch content was high. Sucrose, glucose and fructose concentrations in the medium of E&FS culture system decreased as bulblet formation and enlargement proceeded, suggesting that external sucrose is taken up to by the cells before it is hydrolyzed.

• Journal of Plant Biotechnology
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• v.37 no.1
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• pp.110-114
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• 2010

Highest increase of biomass was observed when tissue-cultured potato (Solanum tuberosum L. cv. Chubaek) shoots were cultured in a liquid medium containing 1/3 MS solution in a 18 L bioreactor, as compared to 1/4 and 1/2 MS solution. The medium containing 1/4 MS solution showed higher increase of shoot biomass than one containing 1/2 MS solution. Potato microtubers were formed when the medium was exchanged with the medium for microtuber formation and incubated under dark condition. The microtubers were observed first at some axillary buds one week after incubation under dark condition and then at most of the axillary buds by the end of 3 weeks. The 1.5 MS liquid medium and $20^{\circ}C$ were optimal conditions. By the end of 6 weeks, more 1,000 microtubers were formed in the 18 L bioreactor. Then, greened microtubers were harvested after one week culture under light condition.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.178-178
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• 2005

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.173-173
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• 2003

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.140-140
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• 2003

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.142-142
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• 2003

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.179-179
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• 2005