Kim, Da-Hye;Park, Sangkyu;Park, Bo-Ra;Lee, Jong-Yeol;Lim, Sun-Hyung
Korean Journal of Breeding Science
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v.50
no.4
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pp.351-363
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2018
In ornamental crops, the color and shape of flowers are one of the important traits. Generally, flower colors are determined by accumulating pigments such as carotenoids, flavonoids, and betalains. Among them, flavonoids are responsible for broad ranges of colors. Chrysanthemums are one of the most popular ornamental crops in the world, and there have been many efforts to change their flower color. In chrysanthemum flowers, cyanidin-based anthocyanin confers pink or red color, whereas terpenoid-based carotenoids are mainly responsible for yellow and green colors. However, blue colored chrysanthemums do not occur in nature. To date, there have been attempts to obtain blue or violet-colored chrysanthemum flowers through the introduction of a novel gene for accumulating delphinidin-based anthocyanins, while other studies have reported changing endogenous metabolites through the reconstruction of flavonoid biosynthesis. Since various transcription factors are involved in the regulation of flavonoid biosynthesis, it is important to understand not only the structural genes, but also the transcription factors required for the modification of flavonoid-based flower color. Therefore, in this paper, we describe the flavonoid biosynthetic pathway and its regulation, and review previous studies on the change in flower color through modification of flavonoid biosynthesis. This effort could be an important milestone in successfully achieving the modification of chrysanthemum flower color by means of plant biotechnology.
Proceedings of the Korean Society of Crop Science Conference
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2017.06a
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pp.83-83
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2017
The National Agrobiodiversity Center (NAS, RDA, Republic of Korea) has continually collected new valuable genetic resources. In this study, we regenerated conserved kidney bean (Phaseolus vulgaris L.) germplasm which couldn't be available because of seed quantity and quality, and we also surveyed their morphological characters for the sustainable utilization. A total of 431 kidney bean accessions were regenerated and 18 morphological traits were surveyed according to the characterization guideline of RDA Genebank. Among the surveyed traits, flowering time ranged from May 23 to September 4 and 73.8% of tested accessions were mainly flowering in June. The maturity time ranged from July 1 to October 15 and main flowering time was July (91.4%). For plant type, 270 accs (62.6%) were climbing type followed by medium type of 86 accs (20.0%) and dwarf type of 65 accs (15.1%). The seed coat colors were various; yellow (34.6%), white (22.3%), brown (17.9%), red (10.7%), black (5.8%), violet (11%), pink (1.4%), navy (0.9%). Principal component analysis indicated that five principal components (PCs) with Eigen values >1 accounted for more than 65.8% variability. The first PC was more related to growth habits such as growth type, flowering time, and plant type. The second and third PCs showed higher values of the pigment characters such as seed coat color, flower color, and pod color. In fourth and fifty PCs, there were the higher positive values of the pod shapes. Our results provided insight into the characteristics kidney beans, thus the utilization basis of kidney beans might be elevated for bio-industry.
Massingue, Armando Abel;Filho, Robledo de Almeida Torres;Fontes, Paulo Rogerio;Ramos, Alcineia de Lemos Souza;Fontes, Edimar Aparecida Filomeno;Perez, Juan Ramon Olalquiaga;Ramos, Eduardo Mendes
Asian-Australasian Journal of Animal Sciences
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v.31
no.4
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pp.576-584
/
2018
Objective: This study aimed to develop a value-added product concerning technological and sensory characteristics changes of the use of mechanically deboned poultry meat (MDPM) as meat replacer in lamb and mutton emulsion-type sausages (mortadella). Methods: Sausages were produced with lamb and mutton and with different contents of MDPM. Six treatments, using lamb or mutton and 0%, 30%, and 60% of MDPM in relation to the meat batter, were produced and analyzed for pH, proximal composition, calcium and residual nitrite content, water activity, 2-thiobarbituric acid reactive substances (TBARS), instrumental color and texture profile. The sensory profile of the mortadella's was also evaluated by acceptance test and check-all-that-applies (CATA) analysis. Results: The MDPM addition increased (p<0.05) fat, residual nitrite and calcium content in the all sausage formulations, but mutton sausage had (p<0.05) higher fat and lower moisture content than lamb sausage. The pH, water activity, TBARS index and color was not affected by MDPM additions, while the mutton sausages were significantly redder (higher $a^*$, $C^*$, and lower $h^{\circ}$) and darker (lower $L^*$) than lamb sausages. Adding up to 60% of MDPM reduced (p<0.05) sausages hardness and chewiness. Overall, the meat replacement by MDPM increased the sausages acceptance, but the mutton sausage with 30% of MDPM replacer were the most preferred. Consumers related that pink color, glossy appearance, poultry meat-like taste, soft texture, juicy and greasy mouth feel to all sausages contain MDPM according to CATA analysis. Conclusion: Mutton from culled ewes can be utilized for mortadella production with 30% replacement of lean mutton and fat by MDPM.
The spectroscopic characteristics of the diffusion of beryllium into sapphire are investigated by UV-Vis and FTIR spectroscopy. The $Be^{2+}$ diffused samples are observed in an immersion filled with methylene iodide. The blue colors were fainteddue tochanges of iron's electrovalence ($Fe^{2+}$${\rightarrow}$$Fe^{3+}$) after heat treatment in an oxidation atmosphere and the pink color appeared due to $Cr^{3+}$ in case of violet sapphire. The blue sapphire containing light brown showed yellow orange color due to reaction of beryllium with trace elements during the beryllium diffusion. From analysis of immersion filled with methylene iodide, we could demonstrate to show yellow orange with concentrated color in the rim of samples. Also, there were some changes to the pre-existed inclusions.
The purpose of this study was to seek the means of enlarging the application of Saek-dong to fashion products by surveying and analysing the recognition and image of Saek-dong in college students. As a research procedure, the bibliographical survey on the meaning and history of Saek-dong was preceded in this study, and the students were examined on the recognition and image of Saek-dong through the questionnaires. The female students were more acquiesced with the Saek-dong and Saek-dong clothing than the male students. And the students thought that the Saek-dong was our original and traditional clothing because it was worn by our ancestors from the earliest years. The word Saek-dong reminded them of red, yellow, blue, green, white and red-brown colors in order of appearance. The most familiar color-arrange to them was red+yellow+dark-brown+green+blue, and the blue, purple, green, red, white color was thought as manly Saek-dong colors and the yellow, red, dark-brown, pink, white was regarded as feminine Saek-dong colors. Saek-dong was primarily associated with the image of Saek-dong clothing and most of the students expressed their feeling about the Saek-dong as 'cute.' Most of the students responded that the practical Hanbok was best illustrated as the most applied clothing of Saek-dong and that the attempt to apply the color and pattern of Saek-dong to other modern artistic products was likely to damage the worth of traditional Saek-dong. When it comes to the matter of applying the design of Saek-dong to the fashion products, male students thought that it could be best applied to the shirts, while female students thought that the design of Saek-dong could best be applied to the personal ornaments.
Streptomyces bobili (YS-40) isolated from soil was tested that it had drug resistance against penicillin, cephalosporin series antibiotics and other antibiotics in the previous paper. The treatment of Streptomyces bobili, (YS-40) with ethidium bromide (EtBr), acriflavine and sodium dodecyl sulfate. (SDS) resulted in the elimination of R-plasmid from the host strain. Minimum growth inhibitory concentrations (MIC) of Hg, Ag, penicillin-G, ampicillin, chloramphenicol, oxytetracycline, streptomycin and kanamycin were found to be 15, 10, > 3, 000, > 100, > 1, 000, > 100, < 5 and < 5$\mu\textrm{g}$/$m\ell$ respectively. Among the curing agents, EtBr was proved to be the most powerful compound for the elimination of R-plasmid in the strain and the elimination rate with EtBr(10$\mu\textrm{g}$/$m\ell$) was about 98%. Optimal pH to. the elimination of R-plasmid was pH 7.0 and the R-plasmid in the cells incubated for 24 hrs was proved to be eliminated most effectively. Aerial mass color, soluble pigment formation and reverse side color were reported to be often the plasmid associated characteristics of the R-plasmid bearing bacteria. But these characteristics of the uncured and cured Streptomyces bobili, (YS-40) showed no changes in the most of the pigment formation media tested in this work.
Stainless steel is being used widely for various purposes due to its good corrosion resistance. There has been much research to produce a colored and corrosion-resistant stainless steel by several methods to use in decorating. In this experiment, we coated TiN(C,O,H)films on the SUS304 substrates with the DC magnetron sputtering system and then studied the texture and color of the films as a function of additional gases being inserted into the system. A (220) texture of TixN film changed to (200) by the addition of either hydrogen or the acetylene and changed further to (111) with an increase of the additional acetylene content. The addition of oxygen gas changed from the texture(220) to (111) to (200). The color of the TixN film changed from gold to gray to green with the increase of the content by the addition of oxygen gas, while it changed to pink and finally to gray with the increased of the content by the addition of acetylene gas. Reflectance increased in the region of short wavelength by the addition of hydrogen, but decreased with the increase of the oxygen and the acetylene content.
To investigate the practical assessing method of pork quality, 302 carcasses were selected randomly to represent commercial conditions and were probed at 24 hr postmortem (PM) by Danish Meat Quality Marbling (MQM), Hennessy Grading Probe (HGP), Sensoptic Resistance Probe (SRP) and NWK pH-K21 meter (NpH). Also, filter paper wetness (FPW), lightness (L*), ultimate pH (pHu), subjective color (SC), firmness/wetness (SF) and marbling scores (SM) were recorded. Each carcass was categorized as either PSE (pale, soft and exudative), RSE (Reddish-pink, soft and exudative), RFN (reddish-pink, firm and non-exudative) or DFD (dark, firm and dry). When discriminant analysis was used to sort carcasses into four quality groups the highest proportion of correct classes was 65% by HGP, 60% by MQM, 52% by NpH and 32% by SRP. When independent variables were combined to sort carcasses into groups the success was only 67%. When RSE and RFN groups were merged so that there were only three groups (PSE, RSE+RFN, DFD) differentiating by color MQM was able to sort the same set of data into the new set of three groups with 80% accuracy. The proportions of correct classifications for HGP, NpH and SRP were 75%, 61% and 35% respectively. There was a decline in predication accuracy when only two groups, exudative (PSE and RES) and non exudative (RFN and DFD) were sorted. However, when two groups designated PSE and non-PSE (RSE, RFN and DFD) were sorted then the proportion of correct classification by MQM, HGP, SRP and NpH were 87%, 81%, 71% and 66% respectively. Combinations of variables only increased the prediction accuracy by 1 or 2% over prediction by MQM alone. When the data was sorted into three marbling groups based on SM this was not well predicted by any of the probe measurements. The best prediction accuracy was 72% by a combination of MQM and NpH.
Choi, Jae Won;Kim, Yong Ha;Moon, Young Mi;Kim, Yoen Jung;Choi, Sik Young
Archives of Plastic Surgery
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v.33
no.5
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pp.612-615
/
2006
Purpose: An ideal bony construct can be divided into two broad categories: (1) the design and fabrication of biodegradable, biomimetic scaffolds that provide correct signals to induce osteogenesis: (2) the identification of an ideal source of osteoprogenitor cells to seed onto the scaffold. We selected poly-glycolic acid as a synthetic scaffold among various scaffolds because of these properties. Meanwhile, culture medium is supplemented with fetal bovine serum(FBS): such serum contains essential elements such as proteins, hormones, growth factors and trace minerals. The composition of FBS can be ideal for various cell growth in vitro. We supposed that we could enhance bone growth at a fractured site if FBS was mixed with synthetic scaffold-PGA. Methods: We cultured human osteoblasts in five different prepared culture dishes made with FBS and PGA mixture. The mixtures contained different ratio of FBS, that is, 0, 1.5, 3, 7, and 10%. We cultured human osteoblasts for seven days and examined the growth and attachment of the cells at the 1st, 3rd, 5th, 7th days, respectively. Results: In the mixture of 0% FBS and PGA, the growth of the cells lasted for one day. In 1.5 and 3% FBS and PGA, the growth of the cells was examined at the 3rd day, then minimally declined at the 5th and 7th days. In 7% FBS and PGA, the growth of the cells lasted for 5 days, then declined at the 7th day. In 10% FBS and PGA, the growth of the cells lasted for 5 days, then declined at the 7th day. Staining status of the osteoblasts with alkaline phosphatase showed pale pink color in 0% FBS and PGA groups, but bright pink color in 1.5, 3, 7, 10% FBS and PGA groups, especially in 3%, 7%. Conclusion: In consequence, the growth of human osteoblast was higher in the mixture of FBS and PGA groups than in pure PGA ones. It is assumed that the mixture of FBS and PGA affects the proliferation of human osteoblasts.
Lee, Jong Nam;Kim, Hye Jin;Kim, Ki Deog;Yoo, Dong Lim;Suh, Jong Taek
Horticultural Science & Technology
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v.30
no.6
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pp.784-787
/
2012
'Gwanha' is new strawberry (Fragaria ${\times}$ ananassa Duch.) cultivar, which was released by Highland Agriculture Research Center, Rural Development Administration, in 2011. In 2007, this cultivar was originated from a cross between 'Sucambodiaberry' and 'Selva' that showed excellent ever-bearing characteristics including continuous pink flowering habit under long-day and high temperature condition. It was named 'Saebong No.4' after examining its characteristic and productivity in summer culture from 2009 to 2011. After characteristic and productivity test in 2011, 'Gwanha' was selected as an elite cultivar. The general characteristics of 'Gwanha' include spreading type, middle vigor, elliptic leaf, and long cluster in growth. The fruit shape is wedge, and the red fruit is edible and unique among the domestic ornamental strawberry cultivars. Flower color of 'Gwanha' is pink, and flower cluster is more appearance than 'Pinkpanda'. The average fruit weight of 'Gwanha' is about 7.8 g and the marketable yield is relatively low because of low marketable rate. It is resistant against fusarium wilt. 'Gwanha' is suitable for four season cultivation as an ornamental strawberry cultivar because it shows continuous flowering habit under long-day and high temperature condition.
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