• Mycobiology
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• 제29권4호
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• pp.205-209
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• 2001

The Korean sawmills have recently recognized the importance of prevention of fungal discoloration due to increased losses in revenue. Before establishing integrated control strategies of fungal discoloration, more complete knowledge about causal organisms is needed. As a first step, we initiated a through survey of fungi colonizing commercially important softwood(Pinus dens flora, Pinus koraiensis, and Pinus radiata) logs and lumber in Korea. In this paper we report results obtained from Japanese red pine(Pinus densiflora) log study. In summer 2000, fungi were isolated from Japanese red pine logs in storage, and identified based on their cultural and morphological characteristics. A total of 595 fungi were isolated, representing 21 genera and 30 species. Mold fungi, mostly Trichoderma species, were the most frequently isolating fungi, representing more than half of all isolates. Dematiaceous fungi represented approximately one fifth of the isolates, and Rhinocladiella atorvirens was the most abundant in all samples. Opiostoma species represented 7% of all isolates from cores planted on malt extract agar(MEA) and the incidence of these species doubled with the addition of streptomycin and cycloheximide to MEA. The results indicate that Japanese red pine sapwood is susceptible to colonization by a variety of fungal species. As a result, control strategies that concentrate on one fungus may have limited success because of interference from competing flora.

• Journal of Ecology and Environment
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• 제32권1호
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• pp.13-18
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• 2009

This study was conducted to investigate ectomycorrhizal (ECM) diversity on Pinus densiflora seedlings in a disturbed pine forest. Pine seedlings less than one year old were collected from disturbed and undisturbed sites in the Mt. Songni region. The belowground ECM fungal communities colonizing P. densiflora seedlings were studied using morphotyping and DNA sequencing. The relative abundance of ECM root tips was significantly higher in the undisturbed sites than in the disturbed sites, and the ECM species diversity was lower in the disturbed sites than in the undisturbed sites. In addition, the ECM community composition was significantly different in the disturbed and undisturbed forest sites.

• Journal of Ecology and Environment
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• 제41권9호
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• pp.265-270
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• 2017

Background: Over the years, pine pollens have been excluded as an allergen due to its relatively large size, low protein content, and waxy hydrophobic layer, despite their abundance. However, recent studies suggest the possibilities of pine pollens being allergens, and it has been reported that allergy symptoms were highly prevalent in areas with considerably large pine forests and high possibility of exposure to the pollen. Therefore, we conducted a comparative analysis of the allergenicities of the pollens from the dominant species of Korean pines, red pine (Pinus densiflora), black pine (Pinus thunbergii), and pitch pine (Pinus rigida), in mice. Methods: The protein composition of the pollens from the three pine species was compared via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The pine pollens and proteins extracted from the pollens were introduced to BALB/c mice by nasal inhalation and application to exposed skin and the IgE produced by the mice were extracted from blood and analyzed via ELISA. Results: SDS-PAGE showed differing protein compositions of the pollens of the three pine species. Analysis of blood IgE compositions showed a similar amount of IgE produced when pollens were applied to skin. In contrast, when mice inhaled the pollens, P. densiflora was shown to induce significantly more IgE production than those of the other two species. Conclusions: The experimental results demonstrate that the pollens of all three South Korean pine species induce IgE production, and this production was more pronounced when the pollens were inhaled than when they were applied to the skin. Of the three species, the pollen of P. densiflora was found to induce the highest level of IgE production.

• Journal of Ecology and Environment
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• 제32권2호
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• pp.97-102
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• 2009

This study was carried out to evaluate litterfall dynamics in three adjacent coniferous tree plantations (larch: Larix leptolepis; red pine: Pinus densiflora; rigitaeda pine: P. rigitaeda) planted in the same year (1963), and growing under similar environmental conditions in the Sambong Exhibition Forests, Hamyang-gun, Gyeongsangnam-do. Litter was collected monthly between July 2006 and June 2008. Needle, broad leaf and total litter inputs followed a similar monthly pattern in the three coniferous plantations. The amounts of needles, flowers, and miscellaneous litter were significantly lower in the larch than in the two pine plantations, while branch litter was significantly higher in the larch than in the two pine plantations. Average total litterfall for two years was significantly higher for the pine (5,475 kg $ha^{-1}\;yr^{-1}$ for red pine and 5,290 kg $ha^{-1}\;yr^{-1}$ for rigitaeda pine) plantations than for the larch (3,953 kg $ha^{-1}\;yr^{-1}$ plantation. Needle litter comprised about 73.1% of total litterfall for the rigitaeda pine, 70.8% for the red pine and 62.9% for the larch plantations. Our results demonstrate that litterfall inputs can be affected by tree species.

• 한국산림과학회지
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• 제24권1호
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• pp.53-61
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• 1974

전남(全南) 신안군(新安郡) 흑산면(黑山面) 홍도리(紅島里)에 위치(位置)하고 있는 홍도(紅島)의 적송집단(赤松集團)을 대상(對象)으르 개체(個體)의 수형(樹型), 분지각(分枝角), 수피색(樹皮色), 추재율(秋材率) 침엽장(針葉長), 수지도(樹脂道)의 위치(位置)와 수(數) 등이 조사(調査)되었다. 홍도(紅島)에 자라는 송류(松類)는 적송(赤松)이 대부분(大部分)으로 필자(筆者)는 조사(調査)도중 단일주(單一株)의 해송치수(海松稚樹)를 발견(發見)하였을 뿐이다. 20년생(年生) 이상의 적송(赤松) 14개체(個體)의 침엽(針葉)을 검경(檢鏡)한바 어느 정도(程度)의 해송인자(海松因子)가 혼입(混入)되어 있었다. 밀엽형개체(密葉型個體)와 소엽형개체(疎葉型個體)를 볼 때 부수지도(副樹脂道)의 수(數)가 현저하게 적은 것이 특징(特徵)이며 특히 소엽형(疎葉型)의 경우 부수지도(副樹脂道)의 수(數)가 1.3 이라는 평균치(平均値)를 보였다. 현 단계로서는 과거부터 홍도(紅島)에 해송(海松)이 있었던 것으로 보기 어렵고 해안일대(海岸一帶)에 분포(分布)하는 해송(海松)으로부터 화분(花粉) 비산공여(飛散供與)되는 것으로 생각된다.

• Journal of the Korean Wood Science and Technology
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• 제37권3호
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• pp.200-207
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• 2009

침전 및 훈증처리한 소나무 재선충병 피해목을 목재 재료로서의 활용가능성을 평가하기 위한 소나무재선충의 밀도 및 종압축강도 시험결과는 다음과 같다. 침전처리 후 소나무와 해송 모두 목초액 및 살선충제 처리의 경우 21일째에, 바닷물 침전의 경우에서는 30일째에 재선충의 밀도가 매우 낮아졌다. 재추출된 재선충을 피해가 없는 정상재에 인위적으로 접종한 경우 6개월경과 후까지 감염되지 않았으며, 소나무와 해송의 평균 기건밀도는 각각 $0.47g/cm^3$, $0.54g/cm^3$, 종압축강도는 각각 $304kgf/cm^2$, $363kgf/cm^2$이었다. 훈증처리 후의 소나무재선충은 수종에 관계없이 3개월까지는 검출되었으며, 그 이후에 출현하는 재선충은 유사 선충으로 판명되었다. 24개월이 지난 소나무와 해송의 평균 기건밀도는 각각 $0.54g/cm^3$, $0.52g/cm^3$, 종압축강도는 각각 $353kgf/cm^2$, $370kgf/cm^2$ 이었다. 본 연구의 결과, 피해목의 목재재료로서의 활용성은 일부 검증되었으며 보다 정확한 검증을 위하여 추후 기타 조직, 물리 및 역학적 성능에 대한 검토가 필요할 것으로 생각된다.

• The Korean Journal of Ecology
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• 제26권4호
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• pp.155-163
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• 2003

In order to analyze the succession process from a pine forest to an oak forest, the tree growth of Pinus densiflora and Quercus mongolica ssp. crispula was monitored in a permanent quadrat for 23 years. The measurements were carried out for the stem diameter (DBH) of Pinus densiflora between 1977 and 1999 and for the height of Quercus mongolica ssp. crispula saplings between 1998 and 2000. The floristic composition and the locations of the individual P. densiflora and Q. mongolica ssp. crispula trees and saplings in the quadrat were recorded. P densiflora and Q. mongolica ssp. crispula individuals were randomly distributed within the quadrat. The relative growth rates (RGR) of DBH in P. densiflora were 0.085 $yr^{-1}$ for large trees and 0.056 $yr^{-1}$ for small trees in 1977. The RGR of height for Q. mongolica ssp. crispula was 0.122 $yr^{-1}$. The growth curve for DBH of P. densiflora was approximated by the logistic equation: $$DBH(t) = 30 {[1+1.16exp(-0.13 t)]}^{-1}$$ where DBH (t) the DBH (cm) in year t and t is the number of years since 1977. The growth in height of P. densiflora and Q. mongolica ssp. crispula was described by following equations: $$H (t) = 20.2 {[1+0.407exp(-0.137 t)]}^{-1} (P. densiflora)$$ $$H (t) = 30 {[1+20.7exp(-0.122 t)}^{-1} (Q. mongolica ssp. crispula)$$ Where H (t) is the tree height (m) in year t and t is the number of years since 1977 in P. densiflora and 1998 in Q. mongolica ssp. crispula. With these equations we predicted that the height of Q. mongolica ssp. crispula increases from 2 m in 1999 to 20 m in 2029. Therefore, Q. mongolica ssp. crispula and P. densiflora will be approximately the same height in 2029. The years required for succession from a pine forest to an oak forest are expected 33 with the range between 23 and 44 years.

• 대한본초학회지
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• 제20권4호
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• pp.1-10
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• 2005

Objectives : We investigated the anti-aging effect on skin with the extract of the needles of red pine, Pinus densiflora. Methods : We measured various effects related to skin such as the anti-oxidant effect, the protection against ultraviolet (UV) irradiation, the inhibition of reactive oxygen species (ROS) generation, the induction of heat shock protein 70 (HSP70), the reduction of matrix metalloproteinase-2 (MMP-2) synthesis and senescent cell. Results : The results were as follows : The extract of the needles of red pine (RP) had the potent anti-oxidant effect and the ROS scavenging effect. Also RP preserved the systemic anti-oxidant enzyme system (superoxide dismutase and catalase) from UVB irradiation. RP protected the cell membrane from the damages induced by UVB irradiation. RP induced HSP70, a mediator of resistance to UVB irradiation. RP reduced the synthesis of MMP-2 induced by UVB irradiation. And RP inhibited the amount of senescent-associated (SA) ${\beta}-galactosidase$ staining, as a marker of replicative senescence. Conclusions : The results of our study indicate that the extract of the needles of red pine, Pinus densiflora, has anti-aging effects on skin.

• Journal of the Korean Wood Science and Technology
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• 제42권1호
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• pp.78-87
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• 2014

울진산 금강송재의 재질발현 수령의 판단기준에 대해 검토하기 위해, 소나무(Pinus densiflora Sieb. et Zucc.)와 울진금강송(Pinus densiflora forma erecta Uyeki) 대표목의 물리적 성질을 조사한 결과, 다음과 같은 결과를 얻었다. 울진금강송의 기건밀도는 소나무재보다 약 20% 이상 높았다. 또한 심재율은 70% 이상이었다. 단위면적($1mm^2$)당 수직수지구의 분포수는 0.3개로서 울진금강송이 소나무보다 약 40% 높았다. 수직수지구의 평균 직경은 소나무 $67.8{\mu}m$, 울진금강송 $67.3{\sim}69.9{\mu}m$로서 수종 간 큰 차이는 없었으며, 연륜수가 증가함에 따라 직경도 함께 증가하는 경향이 나타났다. 수평수지구의 분포수는 울진금강송-1이 소나무와 울진금강송-2 보다 약 55% 높은 값을 나타내었다. 소나무와 울진금강송-2의 수지구 분포수는 연륜이 증가함에 따라 감소하였으나, 울진금강송-1은 연륜 증가와 함께 증가하는 상반된 결과를 나타내었다. 방사조직 분포수는 수종 간의 뚜렷한 차이가 나타나지 않았으며, 동일 수종 내 조재와 만재 사이에서도 차이가 없었다. 연륜이 증가함에 따라 소나무와 울진금강송 모두 방사조직의 분포수가 감소하였다. 방사조직의 세포고는 소나무가 비교적 높았으며, 조재와 만재 사이의 차이는 없었다. 본 연구결과, 울진금강송의 재질발현 기준 수령은 약 150년인 것으로 판단되었다.

• 한국식품영양과학회지
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• 제28권5호
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• pp.973-979
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• 1999

Volatile compounds from twigs, needles and sprouts in Pinus densiflora were extracted with n hexane, diethyl ether or ethanol for 24 hours, and the extracted compounds were separated and identified by gas chromatography and mass selective detector. The kinds and amount of volatile compounds extracted from three parts of Pinus densiflora were different in solvent extraction and the extraction by the modified Liken Nickerson apparatus. The contents of volatile compounds of twigs contained more than those of needles and sprouts, and the volatile compounds were extracted more in n hexane than the others. In the extraction with hexane, the main volatile compounds of twigs were 18.5% pinene, 14.5% limonene, 12.7% pinene and 3.2% myrcene. Sprouts were 16.8% limonene, 4.4% pinene, 4.3% pinene and 1.7% myrcene. Needles contained 14.7% pinene, 5.4% pinene, 2.2% limonene and 0.8% myrcene. The highest yield for pine aroma was shown in the extraction from pine twigs with n hexane, and in this extraction the amounts of pinene, pinene and limonene were 742 g, 1108 g and 922 g per gram sample, respectively.