• 제목/요약/키워드: pigment concentration

검색결과 283건 처리시간 0.019초

Production of Antibacterial Violet Pigment by Psychrotropic Bacterium RT102 Strain

  • Nakamura, Yoshitoshi;Asada, Chikako;Sawada, Tatsuro
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제8권1호
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    • pp.37-40
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    • 2003
  • The antibacterial action of violet pigment, a mixture of violacein and deoxyviolacein, isolated from phychrotrophic bacterium RT102 strain was examined, and the operational conditions for the effective production of violet pigment were studied. The antibacterial activity of the violet pigment was confirmed for several bacteria such as Bacillus licheniformis, Bacillus subtilis, Bacillus megaterium, Staphylococcus aureus, and Pseudomonas aeruginosa, and the high concentration of violet pigment, above about 15mg/L, caused not only growth inhibition but also death of cells. The growth properties of RT102 strain were clarified under various incubation conditions such as pH, temperature, and dissolved oxygen concentration. The maximum violet pigment concentration, i.e. 3.7 g/L, and the maximum productivity of violet pigment, i.e. 0.12 g .L$\^$-1/H$\^$-1/, were obtained in a batch culture of pH 6, 20$^{\circ}C$, and 1 mg/L of dissolved oxygen concentration.

Monascus sp. 의 적색색소생성에 대한 용존산소량의 영향

  • 박노환;성문수;오영숙;정욱진
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2000년도 춘계학술발표대회
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    • pp.267-270
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    • 2000
  • 이차대사산물의 생산에 영향을 미치는 인자 중 통기성의 조절을 통한 Monascus 적색색소의 생산을 증가 시켰다. 높은 산소공급조절은 균체량을 증가 시켰으며, 낮은 산소공급조절은 고농도의 적색색소를 생산하였다. 따라서, 산소 공급량의 조절을 통하여 적색색소 생합성 과정을 활성화 시킬 수 있다.

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微生物의 色素에 關한 硏究(第 5 報) -色素形成에 미치는 界面活性劑의 영향- (Studies on the Microbial Pigment (V) The effect of some detergent on pigment formation in Serratia marcescens strain P)

  • 이호용;조홍범;최영길
    • 미생물학회지
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    • 제22권3호
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    • pp.191-195
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    • 1984
  • In order to study on the pigment formation of Serratia marcescens, the synthesis of prodigiosin was examined in the presence of a wide range of concentration of detergents. A high elevation of pigment formation was obtained in case of the treatment with SDC and SAP. And the population growth of the bacteria was increased by SDC and SAP, in the concentration of optimum concentration of pigment formation. The alkaline phosphatase activity was also increased in the treatment of SAP, SDC and SDS. The possible mechanism of the detergents on enhancement of pigment formation could be explained by an increase of enzyme activity and membrane transport.

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Serratia sp. KH-95에 의한 적색 색소 생산 및 배양학적 특성 (Production of Red Pigment by Serratia sp. KH-95 and its Cultural Properties)

  • 김창호;김승욱;홍석인
    • KSBB Journal
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    • 제13권4호
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    • pp.431-437
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    • 1998
  • Optimal media and cultural conditions for the production of prodigiosin-like pigment were established using Serratia sp. KH-95. Glucose and phosphate(K2PO4) stimulated the cell growth, but inhibited the production of pigment at concentration levels of above 10 g/L and 2.0 g/L, respectively. Addition of soy been oil or rice oil to the production medium accelerated cell growth up to more than 2-3 times, but the production of prodigiosin increased about 15-20% in spite of the good cell growth. The effect of pH on the production of pigment was investigated in a 5 liter-bioreactor. When the pH of culture broth was maintained below 8.0, most of pigment was attached to the surface of cells. When the pH of culture broth was above 8.5, however, about 70% of total pigment was suspended in the supernatant of the broth. The cell growth and production of pigment were inhibited at dissolved oxygen concentration of below 10% of air-saturation.

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홍국의 황색색소 Monascin에 대한 안정성 연구 (Stability of Monascin Pigment Isolated from Monascus purpureus)

  • 박영현;채지민
    • 한국식품위생안전성학회지
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    • 제12권1호
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    • pp.15-19
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    • 1997
  • The stability of monascin yellow pigment isolated from Monascus purpureus was determined over a period of storage for the wide range of pH, various metal ions and antioxidants. The absorption maximum of monascin pigment was 385 nm. Monascin pigment was more stable in acid solutions than in alkaline (pH 9 and pH 11) during storage period. It was also observed the reduction of absorption was occur after 3 days storage. The stability of monascin pigment was not changed by adding the various metal ions of the concentration of 10-4 M, however, it was unstable by adding the Zn2+, Al3+ and Fe3+ of 103- M concentration. The antioxidants. BHA, BHT, cysteine and L-ascorbic acid, have no effects on the stability of monascin yellow pigment. Thus, it may be concluded that the monascin pigment is stable and useful food additives as the natural colorant except for the alkaline food and food containing the Zn2+, Al3+ and Fe3+.

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Analysis on the Pigment Composition of Phytoplankton Assemblages using HPLC (High Performance Liquid Chromatography) in the Adjacent Waters of Nuclear Power Plants in Spring

  • Choi, Hyu-Chang;Kang, Yeon-Shik;Choi, Joong-Ki;Song, Tae-Yoon;Yoo, Man-Ho
    • Journal of the korean society of oceanography
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    • 제39권4호
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    • pp.234-242
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    • 2004
  • The pigment composition and concentration of phytoplankton assemblages using HPLC in the adjacent waters of four nuclear power plants (Yonggwang, Kori, Wolsong and Ulchin) were investigated during the spring blooming in 2004. The mean concentration of chlorophyll a ranged from 563.8 to 2,949.0ng $l^{-1}$, with the lowest concentration at Kori and the highest concentration at Wolsong. Among the carotenoids, the amounts of fucoxanthin and chlorophyll $C_2$ were relatively higher than those of other pigments in the study site. As minor pigments, zeaxanthin, chlorophyll b, 19'-butanoyloxyfucoxanthin, diadinoxanthin, 19'-hexanoyloxyfucoxanthin, chlorophyll $C_3$ and peridinin were detected. The results of pigment composition and concentration showed that diatoms had an important proportion of phytoplankton community when a spring bloom occurred. Cyanobacteria was present relatively low density at the Wolsong and the green alga such as chlorophytes and prasinophytes were abundant at the Yonggwang and Kori, while dinoflagellates characterized by peridinin were common at Ulchin and Kori. The pigment composition and concentration of phytoplankton after passing through the cooling-water system of nuclear power plant were highly variable. No distinct trend of the change of each pigment composition and amount was detected but the variation of fucoxanthin and chlorophyll $C_2$ highly coupled with that of chlorophyll a. We pointed out that the diatom controlled the overall variation of phytoplankton biomass during the spring season.

Particle Size, Morphology and Color Characteristics of C.I. Pigment Red 57:1 : 2. Effect of Salt Milling Process

  • Seo, Hee Sung;Lee, Hyun Kyung;Yoo, Eui Sang
    • 한국염색가공학회지
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    • 제27권4호
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    • pp.245-260
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    • 2015
  • The effect of salt milling process on the crystal size, morphology, and color characteristics of C.I. Pigment Red 57:1 was studied. The primitive morphology and color properties of the pigment after synthesis were studied in the former series work. The size and morphology of primary particles and the second aggregation features should be considered because they are very important to determine pigment quality. We compared the primary morphology of pigment particles before drying with the secondary aggregated morphology of pigment particles after drying and salt milling process. Morphological properties were investigated by particle size analysis, X-ray diffraction, and scanning electron microscopy and color measurement was carried out. Significant reduction in particle size as well as enhanced crystallite size after salt milling process was observed. This result might give a difference in color of the pigment, turning into brighter and more blue-toned red color. It was revealed that synthesis condition affect the morphology and color of the pigment even after milling. Increase in HCl concentration in the synthesis process enhanced crystal size and quality forming bluer-red pigments but an increase in $CaCl_2$ concentration resulted in more amorphous crystals forming darker-red pigments after salt milling.

막분리에 의한 자색고구마 색소의 농축 (Concentration of Pigment Extracted from Purple Sweet Potato by Nanofiltration)

  • 김선재;임종환
    • 한국식품과학회지
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    • 제29권3호
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    • pp.492-496
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    • 1997
  • 자색고구마 색소추출액을 막분리법과 진공농축법을 사용하여 농축하였다. 초기 anthocyanin함량이 1.6 g/L인 색소추출액을 막분리법을 이용하여 5시간 동안 연속 농축시켜 부피의 비로 25배 농축시켰으며, 이 때 조색소의 함량은 10.7 g/L이었다. 농축이 진행되는 동안 고형분의 농도는 계속 증가하였으며, frux는 계속 감소하였다. 막분리에 의한 색소 추출액의 농축과정 중 degradation index (DI)의 변화는 미미하였는데, 이는 이 농축방법이 색소의 변색에 아무 영향을 미치지 않음을 의미한다. 비교실험으로서 진공농축법을 사용하여 부피의 비가 5배로 농축될 때까지 농축하였다. DI값은 $40^{\circ}C$보다 $60^{\circ}C$에서 더 증가하는 경향을 나타냈다. 농축색소액의 total color difference값은 막분리법을 사용하는 경우 그 변화가 미미하였으며, 진공농축법을 사용했을 때에는 $40^{\circ}C$보다 $60^{\circ}C$농축에서 더 증가하였다. 이러한 사실들을 자색고구마색소 추출액을 농축시키는데 낮은 온도의 진공농축법이 효과적이며 ethanol및 citric acid의 재사용 등 경제적인 면으로는 막분리법이 효율적인 방법임을 입증한다.

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자색고구마 Antocyanin 색소 추출액의 유동특성 (Properties of Purple-Fleshed Sweet Potato Antocyanin Pigment Solutions)

  • 이정주;임종환
    • 한국식품저장유통학회지
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    • 제8권1호
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    • pp.102-108
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    • 2001
  • 자색고구마 색소 농축액의 유동특성을 원뿔형 회전점도계를 사용하여 가용성 고형분의 농도 25-65% 및 온도 20-6$0^{\circ}C$의 범위에서 조사하였다. 자색고구마 색소 농축액은 뉴톤성유체의 특성을 나타냈다. 자색고구마 색소 농축액의 점도의 온도에 대한 영향은 Arrhenius 식을 따랐으며, 활성화에너지는 14.23-43.00 kJ/mol로서 농도가 증가함에 따라 직선적으로 증가하였다. 자색고구마 색소 농축액의 점도와 온도 및 농도에 대한 관계를 조사한 바, 일정한 온도에서 용액의 점도는 농도가 증가함에 따라 지수함수적으로 증가하였으며, 그 증가 정도는 온도가 낮을수록 증가하였다.

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Effect of NaCl Concentration and Cooking Temperature on the Color and Pigment Characteristics of Presalted Ground Chicken Breasts

  • Bae, Su Min;Cho, Min Guk;Hong, Gi Taek;Jeong, Jong Youn
    • 한국축산식품학회지
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    • 제38권2호
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    • pp.417-430
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    • 2018
  • This study was conducted to determine the effects of NaCl concentration and cooking temperature on the color and pigment characteristics of presalted ground chicken breasts. Four treatments with different salt concentrations (0%, 1%, 2%, and 3%) were prepared and stored for 7 d prior to cooking. Each sample was cooked to four endpoint temperatures ($70^{\circ}C$, $75^{\circ}C$, $80^{\circ}C$, and $85^{\circ}C$). The salt concentration affected the color and pigment properties of the cooked ground chicken breasts. As the salt concentration increased, the cooking yield and residual nitrite content also increased. However, the samples with 1%, 2%, and 3% NaCl showed similar nitrosyl hemochrome and total pigment contents. Among the products containing salt, the samples with 3% NaCl showed the lowest percentage myoglobin denaturation (PMD) and the lowest CIE $a^*$ values. The cooking temperature had limited effects on the pigment properties of cooked ground chicken breasts. The oxidation-reduction potential and residual nitrite contents increased with cooking temperature, while the PMD, nitrosyl hemochrome, total pigment contents and CIE $a^*$ values were similar in the samples cooked at different temperatures. These results indicated that the addition of up to 2% salt to ground chicken breasts and storage for 7 d could cause the pink color defect of cooked products. However, the addition of 3% NaCl could reduce the redness of the cooked products.