• 생명과학회지
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• 제9권4호
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• pp.493-495
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• 1999

Eicosapentaenoic (EPA, 20:5) and docosahexaenoic (DHA, 22:6) acids of gull eggs were analyzed and the results are summarized as follows: 1. EPA and DHA were 2.25% and 4.35% in the total fatty acid of gull eggs. 2. Triglyceride fractions in the fatty acids had 0.56% in EPA and 1.59% DHA. 3. Diglyceride fractions contained 1.10% EPA and 1.97% DHA. 4. Phospholipid fractions had the most abundant amounts of 4.26% EPA and 8.13% DHA.

• 한국환경보건학회지
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• 제22권2호
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• pp.114-123
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• 1996

Escherichia coli에 copper chloride 50 ppm, manganese chloride 100 ppm, nickel chloride 100 ppm을 각각 처리하여 세포를 배양하는 동안에 이들 세포에서 일어나는 인지질 생합성 및 지방산 조성의 변화를 대조구와 비교하여 분석하였다. 세포의 생장과 total lipid, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, cardiolipin은 대조구조에 비해 금속 화합물 처리구에서 저해되었는데 nickel chloride가 가장 큰 억제 효과를 나타내었다. 그러나 phosphatidylinositol은 금속화합물의 영향을 받지 않았다. 인지질 생합성에 이용된 주요 지방산은 대조구는 palmitic acid(평균 25.47%)와 palmitoleic acid(평균 12.27%)가 인지질 생합성에 도입되었고 copper chloride 처리구는 palmitic acid(평균 30.13%)와 stearic acid(평균 9.12%)로 나타났다. manganese chloride 처리구와 nickel chloride 처리구에서는 모두 palmitic acid(평균 24.16%, 평균 21.77%)와 linoleic acid(평균 9.48%, 평균 11.88%)가 인지질 생합성에 이용된 주요 지방산으로 분석되었다.

• Journal of Plant Biology
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• 제35권4호
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• pp.371-383
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• 1992

Chlorella ellipsoidea에서 분리한 엽록체의 인지질 생합성과 그의 지방산 조성에 미치는 $10^{-3}M$의 IAA(indole acetic acid)와 $2{\times}10^{-8}M의\;GA_3의$ 효과를 분석하였다. 처리구가 대조구에 비하여 생장률, 총 지질, 인지질 함량이 높은 것으로 나타났으며 특히 $GA_3$ 처리구에서는 phosphatidyl-choline 함량이 높은 것으로 분석되었다. Whole cell system에서의 주요 지방산은 대조구에서는 palmitic acid가 27.43% 였으며 IAA 처리구에서는 linolenic acid가 20.25%로 분석되었다. $GA_3$ 처리구에서는 linolenic acid가 23.17%로 인지질 형성에 이용된 주요 지방산인 것으로 분석되었다. 분리한 엽록체에서는 대조구의 주요 지방산은 palmitic acid가 35.67%로 나타났으며 IAA 처리구에서는 linolenic acid가 24.91%, GA3 처리구에서는 linolenic acid가 22.80%로 인지질 형성에 이용된 주요 지방산으로 분석되었다.

• 대한약리학회지
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• 제4권1호
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• pp.17-26
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• 1968

The effects of the essential oil fraction and the fatty acid-fraction of the panax Ginseng on the various lipids (total cholesterol, phospholipid, triglyceride) in serum and liver tissues of the white rats were examined and observed after the administration of the fractions for eight weeks with the following results: 1. Serum total cholesterol level was slightly decreased in the group after giving the essential oil fraction four weeks, whereas the effects in the group after giving itfor eight weeks were increased. Phospholipid level was slightly elevated both in four weeks and in eight weeks, while triglyceride level was elevated after four weeks, whereas it showed nochange at all after eight weeks. In the liver tissues, the cholesterol content was slightly decreased after four weeks and remarkably decreased after the administration for eight week. Phospholipid increased after four week administration and then rather decreased after the eight week administration, while the triglyceride content decreased both in four weeks and in eight weeks. 2. In the group giving the fatty acid-fraction, serum total cholesterol level decreased after the four week administration and then returned to the pre-experiment mean after the eight-week administration, while phospholipid level increased after the four-week administration and then returned to the pre experiment mean. Triglyceride level increased after four week administration of the fraction and rather then decreased after eight weeks. In the liver tissues, total cholesterol content decreased both after four weeks and after eight weeks, while phospholipid level also decreased from four weeks through eight weeks, whereas triglyceride level increased after four weeks, and showed rather decreasing tendency after the eight-week administration.

• 한국식품영양과학회지
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• 제20권6호
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• pp.590-595
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• 1991

In order to investigate polar lipid ingredients and fat쇼 acid compositions in corn embryo, lipids were extracted with n-hexane (HX), pet. ether (PE), chloroform-methanol (2:1, v/v) (CM), dichoromethane-methanol (2:1, v/v DM) and hexane-diethyl ether (5:1, v/v HD). Of the glycolipid in polar lipids were separated by thin layer chormatogarphy (TLC), monoglycosyl diacyglycerol was most efficient with CM, HD, and monoglycosy sterol and monoglycosyl ceramide were similar to five solvents, but HX, PE and DM, HD were somewhat superior, respectively. Of the phospholipid, phosphatidyl inositol was most efficient with CM, DM, and phosphatidyl choline was similar to five solvents as well. Phosphatidyl serine was superior PE, HD, CM to HX, and phosphatidyl ethanolamine was inferior CM to HX. The major fatty acid in the glycolipid was linoleic acid, and it was most efficient with CM the same as plamitic acid, but oleic acid was superior in using HX, PE. The major fatty acids in the phospholipid were palmitic, heptadecanoic aicds, and they were superior in using HX and PE, respectively. Also oleic acid was most efficient with HX and CM, but HD was somewhat inferior.

• 대한수의학회지
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• 제35권4호
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• pp.713-727
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• 1995

The present study was investigated fatty acid composition of the phospholipid in the RBC membrane, liver and microsomal fraction after vitamin E and/or insulin treatment to evaluate the effect of vitamin E on the oxidative stress in STZ-treated rat and BB rat. Results obtained through the experiments were summarized as follows; 1. Effect of vitamin E and/or insulin treatment in STZ-treated rat 1) In the insulin treated group and the combination treated groups of vitamin E with insulin, body weights were increased compared to STZ-treated rat(STZ control group). Especially it was more significantly increased in the combination treated group of high dose vitamin E with insulin. 2) The composition of fatty acids of the phospholipid in RBC membrane, liver and microsomal fractions was shown a decreased C16:1, C18:1, C20:4 and an increased C16:0, C18:0, C18:2 in STZ control group compared to normal control group. In RBC membrane, liver and microsomal fractions after vitamin E with insulin treatment in STZ-treated rat, effect on the composition of fatty acids of the phospholipid was shown the result of a decreased C16:0, C18:0, C18:2 and an increased C16:1, C18:1, C20:4. 3) Hemolysis rate of the RBC to $H_2O_2$ was increased in the STZ control group and it was decreased below the hemolysis level of normal control group by vitamin E treatment. 2. Effect of vitamin E and/or insulin treatment in BB rat 4) Only in microsomal fraction, fatty acid composition was different between insulin treatment group and vitamin E with insulin treatment group. It was increased C16:0 and C18:1, and decreased C18:0 and C18:2 in vitamin E with insulin treatment group: But C20:4 was not different in two groups. These results suggest that the combination treatment of vitamin E and insulin could prevent the oxidative change of fatty acids in P-lipid of the RBC membrane, liver and microsomal fraction in STZ-treated rats and BB rats.

• 한국식품영양과학회지
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• 제23권4호
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• pp.555-560
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• 1994

In order to observe the effects of the fees mixed with the lard and two vegetable seed oils on the fatty acid compositions of liver and brain tissue, the oils mixed with 2.5% lard and various levels of perilla oil and evening primrose oil were administered to the male rats of the Sprague-Dawley for 4 weeks . In the fatty acid composition of liver lipid, saturated fatty acid (SFA) contents were rich in the phopholiipide and cholesteryl ester fraction. Monounsaturated fatty acid (MUFA) contents were rich in the triglyceride fraction and polyunsaturated fatty acid (PUFA) contents were rich in the phospholipid fraction. In the fatty acid composition of liver lipid fractions, according as the contents of mixed perilla oil decreased and the contents of mixed evening primrose oil increased , n -3 PUFA contents tended to decrease and n-6 PUFA contents tended to increase. Fatty acid composition of liver lipid fractions were influenced from the fatty acid composition of the test lipids. In the fatty acid composition of brain phospholipd, PUFA contents (40%) were rich and according as the contents of mixed evening primrose oil increased, the ratio on n-3/n-6 PUFA and eicosapentaenoid acid (EPA) /arachidonic acid (AA) tended to slightly decrease.

• Journal of Nutrition and Health
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• 제29권2호
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• pp.177-187
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• 1996

We studied the effects of fish oil supplementation with low does on the lipid concentration and fatty acid of plasma and the fatty acid composition of plasma phospholipid and erythrocyte of lactating women. The subjects, 18 lactating women, who were exclusively breast-fed their babies were classifed into a control group and 2 fish oil groups according to dose; the subjects of fish oil groups were supplemented with 1.96g/d or 3.92g/d of fish oil, respectively for 2 weeks from 10 to 12 weeks postpartum. All subjects consumed their usual diet at home. Blood sample were collected at the final day of experiment. The plasma HDL-cholesterol level increased significantly by fish oil supplementation. The concentrations of DHA (docesahexaenoic acid) and EPA (eicosapentaenoic acid) in the plasma PC(phosphatidylcholine) and PE (phosphatidylethanolamine)of fish oil groups tended to increase, but not significant. However, the concentrations of DHA and EPA of PC and PE in erythrocyte were not affected by fish oil supplementation. These results demonstrate that fish oil supplementation with low dose does not change the concentration of plasma lipid as well as fatty acid composition in plasma PC and PE and red blood cell obviously. However the increase of plasma HDL-cholesterol level, the reduction of atherogenic index(AI) and the tendency of increase of DHA and EPA concentrations in plasma PC and PE indicate that there may be some beneficial effects on maternal lipid metabolism if fish oil intakes were increased.

• Journal of Nutrition and Health
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• 제29권1호
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• pp.112-121
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• 1996

The study was designed to observe the effect of blend fat calculated from the foods consumed in Korean with those of perilla oil, beef tallow and corn oil on colonic mucosal phospholipid fatty acid composition and the levels of TXB2 and diacylglycerol (DAG) which were known as biomarkers for cancer. Male Sprague Dawley rats, at 7 weeks of age, were divided into control and 1, 2-dimethylhydrazine (DMH)-treated group, and each group was subdivided into four groups. The experimental diets contained one of four dietary fats, blend fat (BF), perilla oil(PO), beef tallow (BT) or corn oil (CO), at 15% (w/w) level. At the same time, each rat was injected with saline for control group or DMH twice a week for 6 weeks to give total dose of 180mg/kg body weight. DMH injection, regardless of the type of dietary fats, significantly increased the levels of PGE2 and TXB2 in colonic mucosal layer compared to control (p<0.01). However, the level of eicosanoids was influenced by the types of dietary fats in both control and DMH group. In control groups, colonic mucosal level of TXB2 was higher in beef tallow group, but lower in perilla oil group compared to that of blend fat (p<0.01). In DMH groups, the level of TXB2 was higher in beef tallow and corn oil groups(p<0.05). The level of PGE2 showed the same trends with TXB2 and beef tallow most significantly increased the level of PGE2. DMH treatment did not influence on tissue fatty acid profile, which was directly reflected by dietary fatty acid composition. Proportions of C18 : 2 in colonic mucosal phospholipid well reflected dietary level of C18 : 2 showing the order CO>BF>PO>BT. The precentage of arachidonic acid(AA) in mucosal phospholipid was the highest by CO adn BT groups and the lowest by PO group. The incorporation of $\alpha$-linolenic acid in colonic mucosal phospholipid in perilla oil group was negatively correlated to the content of AA. Dietary level of C18 : 2 might not be the only controlling factor for the production of eicosanoids in colonic mucosa layer and might function with $\omega$3 fatty acids. The level of DAG was significanlty lower in PO group than that of BT group. Therefore, $\omega$3 $\alpha$-linolenic acid rich perilla oil could be very important dietary sourec in controlling eicosanoid production DAG level in cloln and recommenced to use more often in meal preparation to reduce the risk factor against colon cancer.

• 한국응용과학기술학회지
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• 제31권3호
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• pp.359-366
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• 2014

포화지방산과 인지질(DMPC)혼합 LB막에 대한 전기화학적 특성을 조사하였다. 포화지방산과 DMPC 혼합 단분자 LB막은 ITO glass에 Langmuir-Blodgett법을 사용하여 제막하였다. 전기화학적 특성은 $NaClO_4$ 용액에서 3 전극 시스템 (Ag/AgCl 기준전극, 백금선 카운터 전극 및 LB 필름이 코팅된 ITO 작업 전극)으로 순환전압전류법을 사용하여 측정하였다. 그 결과 포화지방산과 인지질(DMPC)의 LB막은 순환전압전류도표로부터 산화전류로 인한 비가역공정으로 나타났다. 포화지방산과 인지질(DMPC)혼합(몰비 1:1) LB막(C14, C16, C18, C20)에서 확산계수(D)는 0.05 N $NaClO_4$에서 각각 $1.2{\times}10^{-3}$, $2.1{\times}10^{-3}$, $1.4{\times}10^{-4}$ 및 $1.1{\times}10^{-3}cm^2/s$로 산출되었다.