• Title/Summary/Keyword: phenotypic

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A LuxR-type Transcriptional Regulator, PsyR, Coordinates Regulation of Pathogenesis-related Genes in Pseudomonas syringae pv. tabaci (Pseudomonas syringae pv. tabaci 에서 LuxR-type 전사조절자인 PsyR에 의한 병원성 유전자들의 조절)

  • Choi, Yeon Hee;Lee, Jun Seung;Yun, Sora;Baik, Hyung Suk
    • Journal of Life Science
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    • v.25 no.2
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    • pp.136-150
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    • 2015
  • Pseudomonas syringae pathovar tabaci is a plant pathogenic bacterium that causes wildfire disease in tobacco plants. In P. syringae pv. tabaci, PsyI, a LuxI-type protein, acts as an AHL synthase, while primary and secondary sequence analysis of PsyR has revealed that it is a homolog of the LuxR-type transcriptional regulator that responds to AHL molecules. In this study, using phenotypic and genetic analyses in P. syringae pv. tabaci, we show the effect of PsyR protein as a quorum-sensing (QS) transcriptional regulator. Regulatory effects of PsyR on swarming motility and production of siderophores, tabtoxin, and N-acyl homoserine lactones were examined via phenotypic assays, and confirmed by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). Further qRT-PCR showed that PsyR regulates expression of these virulence genes in response to environmental signals. However, an upstream region of the gene was not bound with purified MBP-PsyR protein; rather, PsyR was only able to shift the upstream region of psyI. These results suggested that PsyR may be indirectly controlled via intermediate-regulatory systems and that auto-regulation by PsyR does not occur.

Genetic and phenotypic relationships of live body measurement traits and carcass traits in crossbred pigs of Korea

  • Do, Chang-Hee;Park, Chan-Hyuk;Wasana, Nidarshani;Choi, Jae-Gwan;Park, Su-Bong;Kim, Si-Dong;Cho, Gyu-Ho;Lee, Dong-Hee
    • Korean Journal of Agricultural Science
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    • v.41 no.3
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    • pp.229-236
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    • 2014
  • This study presents the estimates of heritabilities of body measurement traits and carcass traits, and genetic and phenotypic correlations of those traits for crossbred pigs in Korea. Body and ultrasound (A mode: Piglog 105) measurements in 221 pigs including body weight, length, height and width, three back fat thickness at the points of 4th, 14th rib and chine bone, eye muscle area and lean meat percent were collected at the ages of 70, 145 and 180 days and then slaughtered to measure carcass weight, back fat, belly, collar butt, spare rib, picnic shoulder, hind leg, loin, tenderloin, lean meat yield and intramuscular rough fat content in loin. Genetic analysis was done using a multi-trait animal model. Heritabilties of the body measurements were ranged from 0.331 to 0.559 and three measurements of back fat thickness were also high as range varying from 0.402 to 0.475 for the ages of 145 and 180 days. However, eye muscle area was moderate (0.296) at the age of 180 days. Heritabilities of retail cut yields were also high as ranged from 0.387 to 0.474 and of IMF content in loin was 0.499. Heritabilities of the cut percent traits were ranged from 0.249 to 0.488. Important positive genetic and phenotypic correlations were noted for all carcass yield traits (0.298 to 0.875 and 0.432 to 0.922, respectively). IMF showed low negative genetic correlations with carcass yield traits, such as carcass weight, picnic shoulder, hind leg, loin, tenderloin and lean meat yield whereas low positive genetic correlations with back fat, belly, collar butt and spare rib. Loin, tenderloin and lean meat percent showed negative genetic correlations with carcass weight, back fat thickness, collar butt, spare rib and picnic shoulder percent. The four body measurements at the ages of 70, 145 and 180 days had positive genetic correlations with belly, shoulder butt, spare rib, picnic shoulder and hind leg percent, but negative genetic correlations were shown with loin and tenderloin percent except body measurements at 70 days. The results suggest that carcass yield are negatively correlated with intramuscular fat content, which is a major factor deciding pork quality and the yield of loin and tenderloin are not increased as much as increase in body size. However, the proportions of belly and collar butt are increased with the body size. In conclusion, selection strategy should be designed according to the preference on composition of carcass in each country.

Studies on DNA Fingerprint for the Korean Native Chicken (한국 재래계의 유전자 지문에 관한 연구)

  • 여정수;정태완;한재용;최창본;김재우;정선부
    • Korean Journal of Poultry Science
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    • v.20 no.4
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    • pp.209-216
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    • 1993
  • This study was conducted to classify Korean native chicken(KNC) and imported chicken by phenotypic performances and DNA fingerprinting. Two lines, KNC and White Leghorn(WL) , of chicken were maintained in the laboratory of Yeungnam University. Economic traits (body weight, sexual maturity, hen-day egg production, egg weight) and phenotypic characteristics (body-type, head, feather, shank) were checked. The DNA fingerprinting was analyzed for both breeds. The growth rate of the KNC was similar to WLS and sexual maturity of the KNC came later than WL. Hen-day egg production of the KNC was also slightly lower than the WL. The egg weight was about 10g lighter than WL. There was no difference in body weight of female KNC compared to the WL after 28 weeks. The study confirms difference between KNC and WL in DNA fingerprinting as well as its outlook. Thus, we suggest that these should be tested in nationwide districts about chickens known as the KNC using DNA fingerprinting. Then, the confirmed KNC populations should be maintained and used for the genetic improvement. Finally, only confirmed KNC should be in market which induce consumer to seek the KNC by its favorite.

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Phenotypic Trend and Environmental Factors for Carcass Traits in Commercial Pigs (비육돈의 도체성적 변화 추세와 환경적인 요인 분석)

  • Kim, B.W.;Park, J.W.;Kim, H.Y.;Seo, J.T.;Lee, J.G.
    • Journal of agriculture & life science
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    • v.43 no.1
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    • pp.35-41
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    • 2009
  • This study was conducted to analyze the phenotypic trends and environmental factors affecting the following carcass traits : slaughter weight, backfat thickness and carcass grade, in 1,251,572 commercial pigs for 7 years from 1999 to 2005. The average slaughter weight of female, male and barrow were $82.97{\pm}0.008$ $82.00{\pm}0.090$ and $82.79{\pm}0.008$, respectively. The average backfat thickness of female, male and barrow were $19.27{\pm}0.006$, $15.99{\pm}0.072$ and $22.49{\pm}0.006mm$, respectively. The slaughter weight tended to increase over the slaughter years, for backfat thickness remained, even though a significant drop of backfat thickness on 2002($18.75{\pm}0.027$). The slaughter weight was significantly higher in winter($83.29{\pm}0.032$), while the backfat thickness was significantly lower in summer($19.10{\pm}0.026$). The carcass grade A increased from 2000 to 2003, but decreased slightly after 2003. On the contrary, the carcass grade B decreased from 2000 to 2003, but increased slightly after 2003.

A gene responsible for ozone sensitivity (ozrB) in chromosome of escherichia coli B. MQ 1844 (E. coli B. MQ1844 균주의 오존감수성 유전자의 특성)

  • ;Hamelin C.
    • Korean Journal of Microbiology
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    • v.25 no.2
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    • pp.103-109
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    • 1987
  • An ozone-sensitive mutant of Escherichia coli strain B, MQ 1844 is described. Its properties, including high sensitivity to ozone and radiation, inducible filamentation, extensive DNA degradation and impaired DNA synthesis following ozonation, are attributable to a mutation in ozrB, a gene which is cotransducible with malB. Based on differences in phenotypic expression as well as on the particular location of this gene on the bacterial chromosome, ozrB appears as distinct from the other ozone-or radiation-sensitivity genes previously described.

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Differentially expressed genes in sex determination of zebrafish

  • Lee, Jae-Hyun
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 1998.07a
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    • pp.61-62
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    • 1998
  • The effects of rearing temperature on the sex determination of zebrafish were examined to address the questions, "Is it possible to control phenotypic sex artificially; and what genes may be involved in that process\ulcorner". At higher temperature(${32-34}^{\circ}C vs 28.5^{\circ}$C), a dramatic shift of the sex ratio(female 1 : male 9) was found. with DD-PCR cloning, several candidate genes were cloned and analyzed. Further analysis should help to understand sex-determining mechanisms involved in the artificial induction.induction.

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유선 기저세포와 유선 종양발생에서의 역할

  • 김남득
    • Journal of Life Science
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    • v.3 no.2
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    • pp.72-78
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    • 1993
  • 유선에 존재하는 유선상피 기저세포(mammary epithelial stem cells)의 증거와 정상조직 혹은 종양조직 발생에서 이들의 역할들을 요약한다. 유선의 실질조직에 기저세포가 존재한다는 것은 여러 형태의 이식실험에서 설명되었고 또 기저세포의 표현형적 특징들은 여러 가지의 vonoclonal antibodies에 의해 논증되었다. 이들 연구의 결과들은 유선의 기저세포군이 end bud와 유선의 기저층(basal layer)에 존재한다고 제시하고 있다. 그러나 이들 기저세포들이 유선의 preneoplasias와 neoplasias에서도 존재하는지에 대해서는 아직까지 명확한 대답을 주고 있지 않다. 명확한 결론을 얻기 위해서는 기저세포에만 특이적으로 나타나는 phenotypic marker들을 확인해야 하고 또 이들이 변형(transformation)된 세포군에도 표현되는 지를 확인할 때에야 가능할 것이다.

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