• ALGAE
• /
• 제36권4호
• /
• pp.315-326
• /
• 2021

Ion channels are membrane protein complexes mediating passive ion flux across the cell membranes. Every organism has a certain set of ion channels that define its physiology. Dinoflagellates are ecologically important microorganisms characterized by effective physiological adaptability, which backs up their massive proliferations that often result in harmful blooms (red tides). In this study, we used a bioinformatics approach to identify homologs of known ion channels that belong to 36 ion channel families. We demonstrated that the versatility of the dinoflagellate physiology is underpinned by a high diversity of ion channels including homologs of animal and plant proteins, as well as channels unique to protists. The analysis of 27 transcriptomes allowed reconstructing a consensus ion channel repertoire (channelome) of dinoflagellates including the members of 31 ion channel families: inwardly-rectifying potassium channels, two-pore domain potassium channels, voltage-gated potassium channels (K_v), tandem K_v, cyclic nucleotide-binding domain-containing channels (CNBD), tandem CNBD, eukaryotic ionotropic glutamate receptors, large-conductance calcium-activated potassium channels, intermediate/small-conductance calcium-activated potassium channels, eukaryotic single-domain voltage-gated cation channels, transient receptor potential channels, two-pore domain calcium channels, four-domain voltage-gated cation channels, cation and anion Cys-loop receptors, small-conductivity mechanosensitive channels, large-conductivity mechanosensitive channels, voltage-gated proton channels, inositole-1,4,5-trisphosphate receptors, slow anion channels, aluminum-activated malate transporters and quick anion channels, mitochondrial calcium uniporters, voltage-dependent anion channels, vesicular chloride channels, ionotropic purinergic receptors, animal volage-insensitive cation channels, channelrhodopsins, bestrophins, voltage-gated chloride channels H⁺/Cl^- exchangers, plant calcium-permeable mechanosensitive channels, and trimeric intracellular cation channels. Overall, dinoflagellates represent cells able to respond to physical and chemical stimuli utilizing a wide range of G-protein coupled receptors- and Ca²⁺-dependent signaling pathways. The applied approach not only shed light on the ion channel set in dinoflagellates, but also provided the information on possible molecular mechanisms underlying vital cellular processes dependent on the ion transport.

• 한국가스학회지
• /
• 제23권4호
• /
• pp.28-39
• /
• 2019

OGI(Optical Gas Image) 기술에서 주요 가스의 이미지 특성연구는 누출가스의 종류 파악 및 배출량 추정의 기초자료가 될 수 있다. 본 연구의 목적은 산업에서 중요한 methane과 propane 두 탄화수소의 분출가스 이미지의 차이를 관찰하기 위함이다. 적외선을 투과하는 성질을 지닌 석영을 끼운 바람차단막을 제작하여 methane과 propane을 동시에 누출하게 하여 적외선 OGI 카메라로 촬영하고, 그 영상을 분석하였다. 석영차단막이를 활용하여 바람의 영향을 최소화하여 안정된 영상을 확보하였다. 누출 기준값을 1 L/min로 하여 두 탄화수소의 영상을 분석한 결과 OGI 카메라로 쉽게 식별 가능한 거리가 methane의 경우 6 m, propane의 경우 9 m로 나타났다. 적외선 카메라와 누출지점의 거리 1~10 m까지에서 propane의 경우가 methane의 경우 보다 gas plume의 크기가 컸으며 선명하였다. 영상 이미지의 점의 수로 비교하면 propane이 methane보다 평균 3.8배 많았다.

• 한국해양바이오학회지
• /
• 제10권2호
• /
• pp.91-96
• /
• 2018

There are many eutrophic lakes by point and non-point pollution sources such as in dustrial waste water, domestic raw sewage, and mucks. The eutrophic lakes not only cause algal blooms but also destroy the ecosystem in the lakes due to high nutrient concentrations. The purpose of this study was to improve water quality in eutrophic lakes by cultivating microalgae using photobioreactors (PBRs) with selectively permeable mesh (SPM), supplying nutrients in the lake and inhibiting cell leakage by diffusion and water permeability. Chlorella vulgaris, was cultivated using PBRs with SPM installed in Inkyung Lake located in Inha university, Incheon, Korea. When cultivating C. vulgaris, $8.3g/m^2/day$ of average biomass productivity was obtained at 3 days. Furthermore, concentrations of total nitrogen and phosphorus were reduced by 35.7% and 84.2%, respectively, compared to initial condition and water quality in eutrophic lake was improved to oligotrophic environment. These results suggest that microalgal cultivation using PBRs with SPM in the lake could produce microalgal biomass as well as improve water quality by decreasing nutrient concentrations.

• BMB Reports
• /
• 제51권12호
• /
• pp.654-659
• /
• 2018

Antioxidant 1 (ATOX1) protein has been reported to exhibit various protective functions, including antioxidant and chaperone. However, the effects of ATOX1 on the inflammatory response has not been fully elucidated. Thus, we prepared cell permeable Tat-ATOX1 and studied the effects on lipopolysaccharide (LPS)- and 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced inflammation. Experimental results showed that transduced Tat-ATOX1 protein significantly suppressed LPS-induced intracellular reactive oxygen species (ROS). Also, Tat-ATOX1 protein markedly inhibited LPS- and TPA-induced inflammatory responses by decreasing cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) and further inhibited phosphorylation of mitogen activated protein kinases (MAPKs; JNK, ERK and p38) and the nuclear factor-kappaB ($NF-{\kappa}B$) signaling pathway. These results indicate that the Tat-ATOX1 protein has a pivotal role in inflammation via inhibition of inflammatory responses, suggesting Tat-ATOX1 protein may offer a therapeutic strategy for inflammation.

• Animal Bioscience
• /
• 제34권12호
• /
• pp.2012-2022
• /
• 2021

Objective: In most retail centers, primal pork cuts for sale are usually prepared into retail-ready slices and overwrapped with air-permeable plastic film. Also, meat of Korean native black pig (KNP) is reputed for its superior quality, however, its shelf life during retail display has not been studied. Thus, the objective of this study was to evaluate shelf life of prepackaged retail-ready KNP belly and shoulder butt slices during refrigerated display. Methods: Bellies and shoulder butt obtained at 24 h post-mortem from finishing KNP were used. Each belly or shoulder butt was manually cut into 1.5 cm-thick slices. The slices in each cut type were randomly taken and placed on white foam tray (2 slices/tray) overwrapped with polyvinyl chloride film. The retail-ready packages were then placed in a retail display cabinet at 4℃. Shelf life and sensory quality of the samples were evaluated on day 1, 3, 6, 9, 12, and 15 of display. Results: The shoulder butt reached the upper limit (20 mg/100 g) of volatile basic nitrogen for fresh meat after 9 days while, the belly remained within this limit throughout the display time (15 days). Both the cuts reached a thiobarbituric acid reactive substances level of above 0.5 mg malondialdehyde/kg after 9 days. The a^* (redness) values remained unchanged during first 9 days in both cuts (p>0.05). After 9 days, off-flavor was not found in either cut, but higher off-flavor intensity was found in shoulder butt after 12 days. The shoulder butt was unacceptable for overall eating quality after 12 days while, belly still was acceptable after 12 days. Conclusion: The belly showed a longer shelf life compared to the shoulder butt, and a shelf life of 9 and 12 days is recommended for the prepackaged retail-ready KNP shoulder butt and belly slices, respectively.

• Ecology and Resilient Infrastructure
• /
• 제7권4호
• /
• pp.294-299
• /
• 2020

본 연구에서는 팔당 상수원 보호구역내 적정 도로비점오염 저감시설의 계획을 위해 대상 도로구간의 현장조사를 통해서 보호구역내 도로의 특성을 파악하고 이에 적용가능한 도로를 선정하고 시설을 시범적용하여 그 유효성을 검증하였다. 팔당 상수원 보호구역은 도로주변 토양의 배수등급이 높아 침투시설을 지양하고 도로사면을 통한 자연적인 오염원 저감방법을 적용하는 것이 적정하다. 앞으로 도로비점저감시설의 계획 및 설계에서 대상도로의 특성에 대한 분석 후 저감계획을 세우는 것이 적정하며 이는 관련 규정 및 설계 기준에도 반영되어야 할 것이다.

• 상하수도학회지
• /
• 제35권1호
• /
• pp.93-100
• /
• 2021

Forward osmosis (FO) process is a chemical potential driven process, where highly concentrated draw solution (DS) is used to take water through semi-permeable membrane from feed solution (FS) with lower concentration. Recently, commercial FO membrane modules have been developed so that full-scale FO process can be applied to seawater desalination or water reuse. In order to design a real-scale FO plant, the performance prediction of FO membrane modules installed in the plant is essential. Especially, the flux prediction is the most important task because the amount of diluted draw solution and concentrate solution flowing out of FO modules can be expected from the flux. Through a previous study, a theoretical based FO module model to predict flux was developed. However it needs an intensive numerical calculation work and a fitting process to reflect a complex module geometry. The idea of this work is to introduce deep learning to predict flux of FO membrane modules using 116 experimental data set, which include six input variables (flow rate, pressure, and ion concentration of DS and FS) and one output variable (flux). The procedure of optimizing a deep learning model to minimize prediction error and overfitting problem was developed and tested. The optimized deep learning model (error of 3.87%) was found to predict flux better than the theoretical based FO module model (error of 10.13%) in the data set which were not used in machine learning.

• 한국물환경학회지
• /
• 제27권4호
• /
• pp.405-412
• /
• 2011

Increased Non-permeable areas which have resulted from civilization reduce the volume of groundwater infiltration that is one of the important factors causing water shortage during a dry season. Thus, seeking the efficient method to analyze the volume of groundwater in accurate should be needed to solve water shortage problems. In this study, two different watersheds were selected and precipitation, soil group, and land use were surveyed in a particular year in order to figure out the accuracy of estimated infiltration recharge ratio compared to Web-based Hydrograph Analysis Tool (WHAT). The volume of groundwater was estimated considering Antecedent soil Moisture Condition (AMC) and Curve Number (CN) using Long Term Hydrologic Impact Assessment (L-THIA) model. The results of this study showed that in the case of Kyoung-an watershed, the volume of both infiltration and baseflow seperated from WHAT was 46.99% in 2006 and 33.68% in 2007 each and in Do-am watershed the volume of both infiltration and baseflow was 33.48% in 2004 and 23.65% in 2005 respectively. L-THIA requires only simple data (i.e., land uses, soils, and precipitation) to simulate the accurate volume of groundwater. Therefore, with convenient way of L-THIA, researchers can manage watershed more effectively than doing it with other models. L-THIA has limitations that it neglects the contributions of snowfall to precipitation. So, to estimate more accurate assessment of the long term hydrological impacts including groundwater with L-THIA, further researches about snowfall data in winter should be considered.

• 대한본초학회지
• /
• 제36권1호
• /
• pp.41-49
• /
• 2021

Objective : Citrus unshiu peel (Citri Unshius Pericarpium) has been prescribed to suppress coughing and phlegm in Korean medicine. In this study, the effect of ethanol extract of Citrus unshiu peel (CEE) on apoptosis was investigated using cadmium chloride (CdCl2) treated HepG2 cells. Methods : CEE was prepared by extracting 300 g of Citri Unshius Pericarpium in 3 L of ethanol for 72 h. Apoptosis was determined by the TUNEL assay. The mitochondrial membrane potential (MMP) was monitored using the membrane-permeable fluorescent dye Rh123. The expression level of each protein was monitored by Western blot analysis. Results : CEE protected HepG2 cells from apoptosis as determined by the TUNEL assay. A decrease in MMP was observed in cells exposed to cadmium, indicating that mitochondria are involved in the induction of apoptosis. However, CEE recovered the reduction in MMP caused by cadmium. In addition, decreased expression of B-cell lymphoma 2 (Bcl-2), procaspase, and poly(ADP-ribose) polymerase (PARP) by cadmium was increased by CEE. The anti-apoptotic effect of CEE was found to be associated with inhibition of JNK and p38 phosphorylation when examining the expression of phosphorylated MAPK by Western blot. Conclusion : This study showed that CEE exerted anti-apoptotic effects in cadmium-induced HepG2 cells by inhibiting the reduction of MMP and changes in the expression level of apoptotic proteins. These results suggest the potential for CEE to be used for heavy metal-induced liver damage.

• 한국수자원학회논문집
• /
• 제55권1호
• /
• pp.23-32
• /
• 2022

본 연구는 지표수-지하수 연계 2차원 흐름 및 용질거동 수치모의를 통해 혼합대 흐름에 의한 지표수-하상 경계층 용질교환이 지표수 내 용질거동에 미치는 영향을 분석하였다. 먼저, 혼합대 흐름이 부재한 불투수성 하상의 경우 사련 하상파에 의해 발달한 바닥면 근처 재순환 흐름의 포착 효과가 용질 파과곡선 형태를 결정하였다. 반면, 용질이 혼합대로 유입되는 투수성 하상에서는 지표수 대비 유속이 느린 혼합대 흐름에 의해 파과곡선 꼬리 부분 농도가 증가하여 파과곡선 꼬리 연장이 급격히 증가하였다. 또한, 지표수 유속 증가는 바닥면 압력 상승과 함께 혼합대 흐름을 빠르게 하여 파과곡선 꼬리의 연장을 단축시켰다. 이러한 본 연구의 결과는 지표수 내 용질 체류시간 예측에 있어 혼합대 흐름의 중요성을 시사하는 바이다.