• Molecules and Cells
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• 제26권5호
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• pp.436-442
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• 2008

Osteoclasts are multinucleated cells with the unique ability to resorb bone. Elevated activity of these cells under pathologic conditions leads to the progression of bone erosion that occurs in osteoporosis, periodontal disease, and rheumatoid arthritis. Thus, the regulation of osteoclast apoptosis is important for bone homeostasis. In this study, we examined the effects of the Janus tyrosine kinase 2 specific inhibitor AG490 on osteoclast apoptosis. We found that AG490 greatly inhibited osteoclast apoptosis. AG490 stimulated the phosphorylation of Akt and ERK. Adenovirus-mediated expression of dominant negative (DN)-Akt and DN-Ras in osteoclasts inhibited the survival of osteoclasts despite the presence of AG490. Cytochrome c release during osteoclast apoptosis was inhibited by AG490 treatment, but this effect was inhibited in the presence of LY294002 or U0126. AG490 suppressed the pro-apoptotic proteins Bad and Bim, which was inhibited in osteoclasts infected with DN-Akt and DN-Ras adenovirus. In addition, constitutively active MEK and myristoylated-Akt adenovirus suppressed the cleavage of pro-caspase-9 and -3 and inhibited osteoclast apoptosis induced by etoposide. Taken together, our results suggest that AG490 inhibited cytochrome c release into the cytosol at least partly by inhibiting the pro-apoptotic proteins Bad and Bim, which in turn suppressed caspase-9 and -3 activation, thereby inhibiting osteoclast apoptosis.

• 동의생리병리학회지
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• 제16권5호
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• pp.1042-1047
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• 2002

Recently, many natural medicines, which have advantage of less side effects and possibility of long-term use, have been studied for their capacity and effects of anti-bacterial, anti-inflammatory and regenerative potential for periodontal tissues. Cortex Eucommiae, Eupoly phaga, Semen Cuscutae, Halloysitum Rubrum have been traditionally used as medicines for treatment of bone disease in Korea. The objective of the present study is to examine the ability of alkaline phosphatase (ALP) activity in human fetal osteoblast cell line (hFOB1) with several natural medicines. hFOB1 added DMEM/F-12 were cultured with dexamethasone as a positive control, and with each natural medicine. ALP activity was measured by spectrophotometer for enzyme activity and naphthol AS-Bl staining was performed for morphometry. All of the natural medicines induced a higher ALP activity compared to negative control, especially, Cortex Eucommiae increased an ALP activity in all experimental groups (p<0.05). In naphthol AS-Bl staining, all of the natural medicines of this study increased the stained area compared to negative control. Especially, Cortex Eucommiae and Eupoly phaga showed statistical significance compared to negative control (p<0.05). These results indicate that Cortex Eucommiae, Eupoly phaga, Semen Cuscutae, Halloysitum Rubrum have an inducing ability of ALP synthesis on osteoblasts.

• 대한장애인치과학회지
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• 제10권1호
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• pp.22-25
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• 2014

본 증례는 연세대학교 치과대학병원 소아치과에 하악 좌측 구치부 잇몸의 팽융을 주소로 내원하여 파노라마, 컴퓨터 단층촬영 결과 두개 악안면 부위에 이환된 섬유성이형성증으로 진단되었으며, 하악 좌측골의 팽윤과 안모 비대칭 및 좌우 안와 비대칭 소견을 보이고 있었다. 섬유성이형성증의 두개 안면부 병소는 안면기형, 두개 비대칭 뿐만 아니라 안구돌출, 시각 손상, 안면 마비, 삼차 신경통, 청각 손실, 치아 변위 및 부정교합, 맹출지연 등을 유발할 수 있다. 따라서 환아에게 증상이 발생할 수 있음을 보호자에게 설명하고, 검진 및 치료를 위해 구강악안면외과와 협진을 시행하였다.

• 대한장애인치과학회지
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• 제10권2호
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• pp.61-67
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• 2014

The aim of this study was to analyze the clinical characteristics of patients with cognitive and behavioral impairments receiving dental treatment under general anesthesia (GA-dental treatment). From August 2007 to April 2014, information was collected from 475 patients who received GA-dental treatment at the Clinic for Persons with Disabilities, Seoul National University Dental Hospital. The demographic factors (gender, age, disability, medication, GA history, residency type, caregiver, meal type, oral hygiene maintenance, and cooperation level) and dental status (operating duration, DMFT, malocclusion, periodontal disease, tooth defect, and treatment protocol) of the patients were evaluated. DMFT and malocclusion levels were compared among the patients with ANOVA and Sheffe's post-hoc test, and chi-square test, respectively. The correlation between the demographic characteristics and dental status of the patients were analyzed with the Pearson's correction test. The mean age of the patients was 27.1 (7 - 83) years and they had intellectual disabilities (55.4%), developmental disorders (17.9%), brain disorders (16.6%), neurocognitive disorders (4.6%), or others (5.5%). The mean DMFT (DT) was 8.6 (5.2) with a significant difference among the disability types (p<0.05). The incidence of malocclusion was higher in patients with intellectual disabilities and brain disorders than in the other types (p<0.05). The operation time ($191.4{\pm}91.2min$) was correlated with decayed or endodontically-treated teeth (p<0.05). Special needs patients requiring GA-dental treatment showed unfavorable oral conditions. Dental practitioners experience time restrictions and additional costs under a GA setting. Treatment planning and decision-making can be efficiently facilitated by evaluating the clinical characteristics of the patients.

• 한국치위생학회지
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• 제2권1호
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• pp.43-61
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• 2002

The purpose of this study was focus on development of educational objectives by using oral health education in the elementary school from now on. Based on the results of analysis compared with a model of educational objectives and contents by reflecting the programs for analysis were the oral health education programs of the Ministry of health and social Affairs in Korea, and the programs of two states in u.s. in order to construct goals and contents of oral health education in school. The results in this study can be arranged as follows: First, an analysis on the educational objectives of dental health education program resulted that, from a viewpoint of Bloom's taxonomy of educational objectives, in case of domestic, most learning objectives expressed in the handbook of Ministry of Health and social Welfare were given too much emphasis to the cognitive domain of 37 an items in all. Affective domain were appeared the lack of balance of the development domain. Second, the result of analysis for the programs of two states of America, the cognitive domain were the most abundant of 48 an items in all, and were not significantly different to those of Korea in the form of objectives domain or statement. However a big different was shown that they organized the same objectives domain as a pattern of repeating and deepening at every grade. Third, in this study, 10 educational objectives of dental health education were established, based on the result of analyzing those existing educational programs as central figure. Those are oral cavity, brushing, nutrition of teeth, fluoride, flossing, teeth injury, dental decay, periodontal disease, malocclusion, routine dental visits. And general objectives and specific objectives of each learning area were stated and arranged grade by grade.

• 한국학교보건학회지
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• 제6권2호
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• pp.56-68
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• 1993

The primary school age are important period that the children exchange the deciduous teeth for permanent teeth, there are so many outbreak of dental caries which is major oral diseases periodontal disease begins, and malocclusion may be brought about. The purpose of this study is to suggest the fundamental data for enforcement of dental health in primary school age as analyzing the curriculum contents and amount on dental health in primary school course. The data were investigated from 103 volumes of 139 textbooks of second semester in 1992 and first semester i 1993 except some subjects that the quantitative analysis is impossible such as arithmetic a problem in arithmetic, observation, music, fine arts and so on. The contents of all textbooks were analyzed separating into paragraph and picture, the contents for dental health were classified into amount, item, teaching method. The results are as follows: 1) The paragraphs on public health were 3.992 among total paragraphs 69,789 as 5.72%. And the picture were 7.41% as 923 pieces of total 12,457 ones. 2) For dental health, there were 73 paragraphs of 69.789 ones (0.10%) and 42 pieces of 12.457 pictures (0.34%). 3) According to the investigation of curriculum that had the paragraphs related to dental health the most, it turned out to be in order of physical education nature. Right life story, and right life story, righ life, physical education for the picture. And for the grade, it appeared to be in order of sixth, third first grade for the paragraphs and first, third, sixth grade for the pictures. 4) In the attitude and knowledge of item for dental health by grade, it turned out that the paragraph was 76.71%, the behaviour was 23.29%. On the other hand, the knowledge was 35.17% and the attitude was 64.28% for the picture. 5) At the commentary of curriculum in primary school, the goal of health education by each grade was just keep clean and strong teeth, know the role of teeth and the cause of dacayed teeth, and prevent it in second and third grade.

• 대한치과의사협회지
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• 제56권1호
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• pp.42-48
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• 2018

INTRODUCTION The most commonly impacted tooth is the third molar. An impacted third molar can ultimately cause acute pain, infection, tumors, cysts, caries, periodontal disease, and loss of adjacent teeth. Local anesthesia is employed for removing the third molar. This study aimed to evaluate the efficacy and safety of 2% lidocaine with 1:80,000 or 1:200,000 epinephrine for surgical extraction of bilateral impacted mandibular third molars. METHODS Sixty-five healthy participants underwent surgical extraction of bilateral impacted mandibular third molars in two separate visits while under local anesthesia with 2% lidocaine with different epinephrine concentration (1:80,000 or 1:200,000) in a double-blind, randomized, crossover trial. Visual analogue scale pain scores obtained immediately after surgical extraction were primarily evaluated for the two groups receiving different epinephrine concentrations. Visual analogue scale pain scores obtained 2, 4, and 6 h after administering an anesthetic, onset and duration of analgesia, onset of pain, intraoperative bleeding, operator's and participant's overall satisfaction, drug dosage, and hemodynamic parameters were evaluated for the two groups. RESULTS There were no statistically significant differences between the two groups in any measurements except hemodynamic factors (P > .05). Changes in systolic blood pressure and heart rate following anesthetic administration were significantly greater in the group receiving 1:80,000 epinephrine than in that receiving 1:200,000 epinephrine ($P{\leq}01$). CONCLUSION The difference in epinephrine concentration between 1:80,000 and 1:200,000 in 2% lidocaine liquid does not affect the medical efficacy of the anesthetic. Furthermore, 2% lidocaine with 1:200,000 epinephrine has better safety with regard to hemodynamic parameters than 2% lidocaine with 1:80,000 epinephrine. Therefore, we suggest using 2% lidocaine with 1:200,000 epinephrine rather than 2% lidocaine with 1:80,000 epinephrine for surgical extraction of impacted mandibular third molars in hemodynamically unstable patients.

• 구강회복응용과학지
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• 제31권4호
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• pp.378-386
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• 2015

피개 의치는 의치의 안정과 유지력 증가라는 장점을 갖지만 지대치가 우식 또는 치주질환에 이환되기 쉬우며 의치의 두께가 얇아짐으로써 의치가 파절되기 쉽다는 단점도 갖고 있다. 자성 어태치먼트 피개 의치는 수직적 유지력은 크지만 수평적 유지력은 작으므로 지대치나 임플란트에 덜 위해한 힘을 가하며, 다른 기계적 유지장치에 비해 마모나 파손 등이 적다는 장점이 있다. 피개 의치에서 나타나는 의치의 파절은 의치상의 두께가 얇아져서 발생하기도 하지만, 연조직과 경조직, 임플란트와 자연치의 압하량 차이로 인해 발생하기도 한다. 이를 보상하기 위해 silicone ball이 자석 안에 삽입되어있는 자성 어태치먼트가 개발되었으며, 이를 이용해 피개 의치를 제작한 증례에서 우수한 결과가 나타나 보고하고자 한다.

• International Journal of Oral Biology
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• 제35권3호
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• pp.113-119
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• 2010

Porphyromonas (P.) gingivalis lipopolysaccharide (Pg LPS) is the major pathogenic component of periodontal disease. In this study, we have attempted to determine the expression profiles of the signal transduction pathway genes induced by Pg LPS in comparison with Escherichia (E.) coli LPS (Ec LPS). DC2.4 cells were treated for two hours with $1\;{\mu}g/ml$ of Pg LPS or $0.5\;{\mu}g/ml$ of Ec LPS. The total RNA from these cells was then isolated and reverse-transcribed. Gene expression profiles were then analyzed with a signal transduction pathway finder GEArray Q series kit and significant changes in expression were confirmed by real-time PCR. The microarray results indicated that several genes, including Tnfrsf10b, Vcam1, Scyb9, Trim25, Klk6, and Stra6 were upregulated in the DC2.4 cells in response to Pg LPS treatment, but were downregulated or unaffected by Ec LPS. Realtime PCR revealed that the expression of Trim25, Scyb9 and Tnfrsf10b was increased over the untreated control. Notably, Trim25 and Tnfrsf10b were more strongly induced by Pg LPS than by Ec LPS. These results provide greater insight into the signal transduction pathways that are altered by P. gingivalis LPS.

• 치과방사선
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• 제17권1호
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• pp.271-278
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• 1987

The purpose of the present study was to investigate associations between periapical and bitewing techniques by assessing the crestal alveolar bone. This article also reports the ability of these two techniques to correctly detect evidence of interproximal dental caries, and comparison between the interproximal overlapping of teeth. Bitewing and periapical radiographs were used from posterior quardants of 243 dental students in Seoul National University. The distance from cemento-enamel junction to the alveolar crest (CEJ-AC) was measured for each proximal surface from the distal of cuspid to the distal of second molar. Data were arranged according to the proximal surface examined, and bitewing and periapical measurements were compared using paired tests. The obtained results were as follows: 1. In maxilla, a significant t ratio with a P value of 0.05 or less reached for 100% and in mandible, reached for 94%. 2. The anatomic limitations imposed on periapical radiographic technique, most often result in somewhat foreshortened radiographic images. This situation would tend to be accentuated by the anatomical restrictions of the hard palate. 3. Consequently, since the significant differences frequently exist between measurements obtained from bitewing and periapical techniques, it is important to define which technique is used. 4. The number of the interproximal overlapping was the largest medial side of the maxillary second molar, while the smallest at the distal side of the mandibular second premolar. And the overall number of the interproximal overlapping was more (538) in the periapical technique than in the bitewing technique (372). 5. The interproximal dental carious lesions were detected more (74) on the bitewing films than on the periapical ones (23). The fact was resulted from the small number of interproximal overlapping and relative easi- ness of obtaining horizontal angulation in taking the bitewing radiographs.