• 생약학회지
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• 제38권2호통권149호
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• pp.170-175
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• 2007

Activated microglia by neuronal injury or inflammatory stimulation overproduce nitric oxide (NO) by inducible nitric oxide synthase (iNOS) and reactive oxygen species (ROS) such as superoxide anion, resulting in neurodegenerative diseases. The toxic peroxynitrite (ONOO$^-$), the reaction product of NO and superoxide anion further contributes to oxidative neurotoxicity. We tried to evaluate the effects of two kinds of varieties of Perilla frutescens var japnica Hara on the NO production in lipopolysaccharide (LPS)-activated microglia. The perilla cultivars of Namcheondeulkkae (NC) and Boradeulkkae (BR) were developed by pure line from the local variety and by a cross between 'deulkkae' and 'chajogi', respectively. Spirit, hexane, chloroform and butanol fractions of the leaves of NC and BR inhibited the production of NO in LPS-activated microglia. The fractions of BR showed stronger activity than NC and the spirit extracts was the most potent in both cultivars. The solvent fractions of BR suppressed the expression of protein and mRNA of iNOS in LPS-activated microglial cells. Moreover, the extracts of NC and BR showed the activity of peroxynitrite scavenging in cell free bioassay system. These results imply that Namcheondeulkkae and Boradeulkkae might have neuroprotective activity through the inhibition of NO production by activated microglial cells and peroxynitrite scavenging activity.

• 한국토양비료학회지
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• 제32권2호
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• pp.192-198
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• 1999

소형 플라스틱 포트에 토양 3 kg씩 충진하고 붕소처리를 들깨 0, 1, 3, 5, 7, $10mg\;kg^{-1}$, 참외와 오이 0, 5, 10, 15, $20mg\;kg^{-1}$ 해당량을 붕사로 각각 시용하였다. 약 30일간 재배하여 생육기간중 작물의 피해증상을 조사하고, 수확후 건물중 및 식물체중의 붕소함량을 조사한 결과는 다음과 같다. 1. 들깨는 $1mg\;kg^{-1}$, 참외는 $5mg\;kg^{-1}$, 오이는 $10mg\;kg^{-1}$농도 처리량부터 잎의 가장자리에서 황록색을 띠였으며 심 한 경우에는 황갈색으로 고사되었다. 2. 수량은 토양의 붕소시용 농도가 들깨 $3{\sim}5mg\;kg^{-1}$에서, 참외 및 오이의 $5{\sim}10mg\;kg^{-1}$수준에서 감수되었다. 3. 토양중 붕소의 처리농도가 높아질수록 들깨, 참외 및 오이중의 붕소 함량이 높아졌으며 줄기 및 뿌리보다 잎이 높았으며 잎중에서 가장자리 부위가 중심부보다 높게 나타났다. 4.붕소함량이 높은 잎의 가장자리에서의 질소, 인산, 칼리, 칼슘, 마그 네슘, 나트륨, 철 망간, 구리 및 아연 등의 함량은 일정한 경향을 보이지 않았다. 5. 시험 후 토양중의 붕소함량은 높은 처리농도에서처리농도보다 높게 나타나 표토에 이동 축적되었다.

• Journal of Nutrition and Health
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• 제32권5호
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• pp.540-551
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• 1999

This study was performed to investigate the effects of dried leaf powders and ethanol extracts of Perilla frutescens(perilla leaf), Artemisia princeps var. orientalis(mugwort) and Aster scaber(chamchui) on lipid metabolism and antioxidative capacity in rats. Forty-nine male Sprague-Dawley rats weighing 105.9$\pm$1.7g were blocked into seven groups according to body weigth and raised for four weeks with diets containing 5% dried powders of perilla leaf, mugwort and chamchui, or ethanol extracts from the same amount of each dried leaf powder. Food intake was higher in the control and chamchui powder groups than the other remaining groups. Weight gain was not significantly different among all experimental groups. Food efficiency ratio was highest in the perilla leaf powder group. Plasma total lipid and cholesterol, liver total lipid and triglyceride levels were highest in the perilla leaf powder group. In contrast, dried powders of mugwort and chamchui showed hypolipidemic effects in plasma and liver. Plasma and liver TBARS levels in both dried powder and ethanol extract groups of the three plants were lower than control. There was also no significant difference between corresponding dried powder and ethanol extract groups. Catalas and GSH-Px activities in erythrocyte and liver were not different among all the experimental groups. However, SOD activities were significantly different among the esperimental groups. In erythrocyte. SOD activiteis of all dried powder and ethanol extract groups except the perilla leaf powder group were higher than control, and the chamchui power group showed the highest activity among them. In liver, all the plant groups showed higher SOD activities than control. There was no significant difference between corresponding dried powder and ethanol extract groups. In conclusion, the dried chamchui powder and liver. All the dried plant powder and ethanol extract were effective in decreasing the TBARS levels of liver and particularly plasma. Among the antiocidative enzymes, SOD activity was most responsive to the experimental diets. All the plant groups showed increases in liver SOD activities and there were more increments in dried powder groups. Since the effects of dried powder groups on lipid metabolism were better than those of extract groups, it is plausible that the high dietary fiber level in dried powders was effective. Antioxidative effects were not significantly different between corresponding dried power and extract groups, and it was thought that ethanol extracts from three plants also had similar antioxidative effects as dried powderers.

• 방사선산업학회지
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• 제3권1호
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• pp.13-18
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• 2009

In earlier investigations, seeds of Perilla frutescens(L.) Britt. cv. Chookyoupjaso were irradiated with 200 Gy gamma ray to generate mutagenesis. The aim of this study is to investigate the effects of a hexane extract from Perilla frutescens (L.) Britt. cv. Chookyoupjaso mutant 45 on the actions of anti-inflammatory activity on inducible nitric oxide synthase, and an identification of the major active compound. The hexane extract from P. frutescens exhibited activity of inhibition of a NO production ($IC_{50}$, $295.1{\mu}g\;ml^{-1}$). The hexane extract was further divided into subfractions by silica-gel chromatogarphy. Inhibition of the NO production by various fractions was assayed in LPS-stimulated RAW 264.7 cells. Among the seven fractions, the 5th fraction was the most effective ($IC_{50}$, $19.5{\mu}g\;ml^{-1}$). The 5th fraction suppressed the expression of protein of iNOS in LPS-induced RAW 264.7 cells, and GC/MS analyses showed that isoegomaketone is a major bio-active compound in the 5th fraction. The result indicated that isoegomaketone has a good potential to be developed as an anti-inflammation agent.

• The Plant Pathology Journal
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• 제17권4호
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• pp.236-241
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• 2001

Serve outbreaks of anthracnose were observed on perilla plants grown in greenhouses and open fields in several locations in Korea during the disease survey from 1997 to 2000. A total of 53 isolates of Colletotrichum spp. and Glomerella sp. was obtained from diseased perilla plants and identified based on their morphological and cultural characteristics. Forty isolates were identified as Colletotrichum gloeosporioides, three isolates as C. coccodes, five isolates as C. dematium, and the other five isolates as Glomerella cingulata, the teleomorph of C. gloeosporioides. All isolates of C. gloeosporioides tested by artificial inoculation were strongly virulent on perilla plants, but isolates of the other species were weakly or not virulent. Anthracnose symptoms induced on the perilla plants by artificial inoculation with the isolates of C. gloeosporioides were similar to those observed in the fields. This study revealed that C. gloeosporioides is the main causal fungus of perilla anthracnose.

• Natural Product Sciences
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• 제22권4호
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• pp.259-262
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• 2016

The content analysis of fatty acids in Perilla cultivars and commercial oils is conducted through gas chromatography with a flame ionization detector. Results show that Perilla cultivars, such as Deulsaem and Daesil, contain high amounts of ${\alpha}-linolenic$ acid (262.22 and 261.97 mg/g, respectively). Among commercial oils, Perilla oil contains a higher amount of ${\alpha}-linolenic$ acid (515.20 mg/g). Accordingly, ${\alpha}-linolenic$ acid is a major fatty acid of Perilla cultivars and oil. Therefore, Perilla cultivars could be used as a food supplement for nutritional and pharmaceutical purposes.

• 한국자원식물학회지
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• 제32권6호
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• pp.743-751
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• 2019

Sclerotinia rot, caused by Sclerotinia sclerotiorum, is a devastating disease that poses a serious threat to perilla production in Korea. Identifying effective sources of resistance offers long term prospects for improving management of this disease. Screening disease resistant genetic resources is important for development of disease-resistant, new cultivars and conduct related research. In the present study, perilla germplasm were screened in vitro against S. sclerotiorum using detached leaf method. Among 544 perilla accessions, two were highly resistant (IT226504, IT226533), five were resistant (IT226561, IT226532, IT226526, IT226441, and IT226589), five were moderately resistant (IT226525, IT226640, IT226568, IT220624, and IT178655), 16 were moderately susceptible, 31 were susceptible, and 485 were highly susceptible. The resistant accessions in this study could serve as resistance donor in the breeding of Sclerotinia rot resistance or subjected to selection procedure of varietal development for direct use by breeders, farmers, researchers, and end consumers.

• 식물병연구
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• 제22권3호
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• pp.184-189
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• 2016

고부가가치 작물인 참깨와 들깨를 뿌리혹선충이 감염된 시설재배지에 윤작작물로 추천하기 위하여 참깨 10품종, 들깨 10품종, 총 20품종을 고구마 뿌리혹선충과 땅콩 뿌리혹선충을 대상으로 저항성을 검정하였다. 참깨 10품종 모두 M. incognita에는 저항성이나 M. arenaria에는 감수성이었다. 들깨 10품종은 모두 두 종류의 뿌리혹선충에 모두 저항성이었다. 참깨와 들깨를 시설재배지에 윤작작물로 사용하고자 할 때, 참깨는 시설재배지에 감염되어있는 뿌리혹선충의 종을 먼저 동정하여, M. arenaria가 감염된 경우에는 사용할 수 없고, M. incognita가 감염되어 있을 경우에는 윤작작물로 사용이 가능하다. 들깨는 2종의 뿌리혹선충에 모두 저항성으로 이들 선충이 감염된 시설하우스에서 윤작작물로 사용이 가능하다.

• 한국균학회지
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• 제40권3호
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• pp.177-178
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• 2012

경상남도농업기술원 시험포장에 재배중인 들깨에서 S. rolfsii에 의한 흰비단병이 발생되었다. 병징은 줄기가 수침상으로 물러지고 갈색으로 썩으면서 서서히 시들어 말라 죽었다. 병반부와 지제부의 토양 표면에 흰색의 곰팡이가 발생하고 갈색의 작은 둥근 균핵을 많이 형성하였다. 균핵의 크기는 1-3 mm이며 균사의 폭은 4-9 ${\mu}m$였다. 균사특유의 clamp connection이 관찰되었다. 균사생육과 균핵형성 적온은 $30^{\circ}C$이었다. 이와 같이 들깨에서 발생한 병징과 병원균의 균학적 특징을 근거로 이 병을 Sclerotium rolfsii Saccardo에 의한 들깨 흰비단병으로 명명하고자 제안한다.

• 생명과학회지
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• 제13권6호
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• pp.782-787
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• 2003

Perill(Perilla frutescens var. japonica $H_{ARA}$), “Ipdlkkae 1” was tested for the yield components in accordance with several different application levels, seeding methods and seeding dates. The results were summarized as follows: Application of fertilizer remarkably increased leaf number and leaf weight. Specially nitrogen showed the most significant effect. The best combination of NㆍPㆍK for cultivation of perilla was investigated ill three leveles of 6kg /10a, 9kg/10a, and 10kg/10a. The planting density in $10cm\times10cm/m^2$, and the seeding date on December 23 were found as the best results. However, $10cm\times7cm/m^2$ planting was better to get heavy leaf.Perill(Perilla frutescens var. japonica $H_{ARA}$), “Ipdlkkae 1” was tested for the yield components in accordance with several different application levels, seeding methods and seeding dates. The results were summarized as follows: Application of fertilizer remarkably increased leaf number and leaf weight. Specially nitrogen showed the most significant effect. The best combination of NㆍPㆍK for cultivation of perilla was investigated ill three leveles of 6kg /10a, 9kg/10a, and 10kg/10a. The planting density in $10cm\times10cm/m^2$, and the seeding date on December 23 were found as the best results. However, $10cm\times7cm/m^2$ planting was better to get heavy leaf.