• Journal of the Korean Society of Marine Environment & Safety
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• v.19 no.5
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• pp.483-490
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• 2013

In this study, we analyzed AIS measured data for ten days by selecting the four main ports with many ships arriving in the national ports. The peak time congestion of the main ports, calculated by survey research, was about 3.8-5.7 times higher than the hourly average congestion. This is very different from the results of the advanced research, evaluating the marine traffic congestion of the Ulsan main port based on the existing Port-MIS statistical data, which showed a peak time congestion of about 1.7 times higher than the hourly average. This identifies the problem of distorting the traffic characteristics of the current passage. Therefore, in order to evaluate marine traffic congestion, it would be more appropriate to calculate it based on survey research, rather than Port-MIS statistical data.

• Proceedings of the KIEE Conference
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• 2002.11a
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• pp.260-262
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• 2002

The determination of DNA hybridization can apply the molecular biology research. clinic diagnostics. bioengineering, environment monitoring, food science and other application area. So, The determination of hybridization is very important for the improvement of DNA detection system. In this study, we report the characterization of the DNA hybridization by the electricalchemical methods. The probe oligonucleotide was used to determine the amount of target oligonucleotide in solution using Methylen Blue(MB) as the electrochemical indicators. The cathodic peak currents($I_{peak}$) of MB were linearly related to the concentration of the target oligonucleotide sequence in the range $1[{\mu}M]{\sim}0.1[{\mu}M]$. The detection limit of this approach was 0.01[nM]. As a result, the match oligonucleotide(CR-1) was most stable state and the peak of redox current measured by DNA hybridization detection sensors by using electrochemical method seem to be similar to 1-mer terminal mismatch oligonucleotide(MR-3). The MR-2, MR-3, MR-22 and MR-33 have each mismatching sequence of central and terminal. With this set the role of point mutations was to be investigated. Terminal mismatch oligonucleotide (MR-3, 33) is shown more stable state than central mismatch oligonucleotide(MR-2, 22). And 1-mer mismatch oligonucleotide(MR-2 or 3) is shown more stable state than 2-mer mismatch oligonucleotide(MR-22 or 33).

• Journal of Korean Society on Water Environment
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• v.27 no.2
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• pp.167-177
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• 2011

Billions of barrels of briny produced water are generated in the United States every year during oil and gas production. The first step toward recovering or reusing this water is to remove the hazardous organics dissolved in the briny produced water. Biological degradation of hazardous volatile compound could be possible regardless of salinity if they were extracted from briny water. In the current work, the effectiveness of a vapor phase biofilter to degrade the gas-phase contaminants (benzene, toluene, ethylbenzene and xylenes, BTEX) extracted from briny produced water was evaluated. The performance of biofilter system responded well to short periods when the BTEX feed to the biofilter was discontinued. To challenge the system further, the biofilter was subjected to periodic spikes in inlet BTEX concentration as would be expected when it is coupled to a Surfactant-Modified Zeolite (SMZ) bed. Results of these experiments indicate that although the BTEX removal efficiency declined under these conditions, it stabilized at 75% overall removal even when the biofilter was provided with BTEX-contaminated air only 8 hours out of every 24 hours. Benzene removal was found to be the most sensitive to time varying loading conditions. A passive, granular activated carbon bed was effective at attenuating and normalizing the peak BTEX loadings during SMZ regeneration over a range of VOC loads. Field testing of a SMZ bed coupled with an activated carbon buffering/biofilter column verified that this system could be used to remove and ultimately biodegrade the dissolved BTEX constituents in briny produced water.

• Journal of Surface Science and Engineering
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• v.55 no.2
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• pp.91-95
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• 2022

ZnO single layer (60 nm thick) and ZnO with Ag interlayer (ZnO/Ag/ZnO; ZAZ) films were deposited on the glass substrates by using radio frequency (RF) and direct current (DC) magnetron sputter to evaluate the effectiveness of Ag interlayer on the optical visible transmittance and the conductivity of the films. In the ZAZ films, the thickness of ZnO layers was kept at 30 nm, while the Ag thickness was varied as 5, 10, 15 and 20 nm. In X-ray diffraction (XRD) analysis, ZnO films show the (002) diffraction peak and ZAZ films also show the weak ZnO (002) peak and Ag (111) diffraction peak. As a thickness of Ag interlayer increased to 20 nm, the grain size of the Ag films enlarged to 11.42 nm and the optical band gap also increased from 4.15 to 4.22 eV with carrier concentration increasing from 4.9 to 10.5×10²¹ cm^-3. In figure of merit measurements, the ZAZ films with a 10 nm thick Ag interlayer showed the higher figure of merit of 4.0×10^-3 Ω^-1 than the ZnO single layer and another ZAZ films. From the experimental result, it is assumed that the Ag interlayer enhanced effectively the opto-electrical performance of the ZAZ films.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.2
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• pp.279-284
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• 2011

The objective of this study was to evaluate the effects of storage temperature and time on the quality parameters of eggs from laying hens at peak production. A total of 576 eggs were obtained from Lohmann Light-Brown hens, which were collected 3 times when the hens were 26, 27, and 28 weeks old. The fresh eggs were collected and measured within 2 h of being laid. Samples of 48 eggs each were stored in chambers for 2, 5, or 10 d inside a refrigerator ($5^{\circ}C$), at room temperature ($21^{\circ}C$), and at a high temperature ($29^{\circ}C$). As the storage temperature and time increased, egg weight, percentage of albumen, Haugh unit (HU), and yolk color significantly (p<0.001) decreased. In addition, egg shell weight, shell percentage, and albumen weight significantly (p<0.001) decreased with storage time. Yolk weight, yolk percentage, and albumen pH significantly (p<0.001) increased with increasing storage temperature, and yolk pH significantly (p<0.001) increased with increasing storage time. When the storage temperature was increased to $29^{\circ}C$, egg weight loss dramatically increased from 1.74 to 3.67% at 5 and 10 d of storage time, respectively. With the exception of the $5^{\circ}C$ storage temperature, HU dramatically decreased according to storage time and temperature, decreasing from 91.3 to 72.63 at $21^{\circ}C$ and from 87.62 to 60.92 at $29^{\circ}C$ during 10 d of storage; however, this decline was not found at $5^{\circ}C$. A rapid increase in albumen alkalinity was observed even after just 2 d of storage regardless of the storage temperature. Interactions between storage time and temperature were significant (p<0.001) with respect to egg weight loss, egg shell weight and percentage, albumen weight and percentage, yolk weight and percentage, albumen and yolk pH, HU, and yolk color. The results of the current study indicated that eggs from laying hens at peak production had significant deterioration of internal quality with increasing storage temperature and time. The results suggest that egg weight loss, albumen pH, and HU are parameters that are greatly influenced by the storage temperature and time of eggs from hens at peak laying.

• Korean Journal of Veterinary Research
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• v.32 no.2
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• pp.157-164
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• 1992

Changes in the both inward current and conductance of membrane by the fertilization were observed using the one microelectrode voltage clamp(or switch clamp) technique. Unfertilized eggs and both 1- and 2-cell stage eggs after fertilization were donated from the superovulated mouse (ICR, more than 6 weeks old) treated with PMSG(pregnant mare serum gonadotropin, Sigma) and HCG(human chorionic gonadotropin, Sigma) and naturally mated ones, respectively in this experiment. Membrane potential was held at -90mV and the voltage step was applied from -80mV to 50mV with interval of 10mV or 20mV for 300ms. since both of amplitudes and time courses in the membrane currents were various according to the states of cells and clamping condition, results were presented by their $averages{\pm}SEM$(standard mean error)and ratios or percentages. Inward currents began to appear in response to the step depolarization from -60mV and reached its maximum at -50mV. However, since the potential was not clamped evenly during the voltage step, current-voltage(I-V) relationship might be positively shifted 10 or 20mV. From the steady-state currents plotted in the I-V curve, outward rectification was markedly observed. Peak inward currents$(i_{in})$ at -50mV were $-0.62{\pm}0.23nA$(n=4),$-0.52{\pm}0.25nA$(n=5) and $-0.37{\pm}0.25nA$(n=6), in the 1-cell stage, 2-cell stage fertilized eggs and in the unfertilized eggs, respectively. Pure inward current (difference between steady-state and peak, $i_{in. pure}$) were $-1.01{\pm}0.23nA$, $-0.69{\pm}0.43nA$ and $-0.68{\pm}0.29nA$, respectively in the 1-cell stage fertilized eggs, unfertilized eggs and 2-cell stage fertilized eggs. These results suggested that the outward current in fertilized eggs of 2-cell stage was more increased than those in the unfertilized eggs. Pure inward currents in the all stages of eggs showed a similar fashion in the I-V relationship from -50mV to 50mV and reversal potential at 50mV. Time constant of inactivation$({\tau})$ in the inward current was decreased as the membrane potential was depolarized in the unfertilized and 2-cell stage eggs but in the 1-cell stage eggs t was not likely to be affected significantly. Slope conductances were 14.2nS, 8.9n5 and 7.7nS in the 1-cell, 2-cell stage fertilized eggs and the unfertilized eggs, respectively. Membranes between two cells within a zona pellucida seem to be electrical-connected in the 2-cell stage eggs from the observation made in the analysis for the electronic spread and decay to the current stimuli. Both of inward current and membrane conductance were increased after fertilization in the mouse eggs. Inward current seems to be carried by the same ion or through the same channels up to the 2-cell stage and ion that carried inward current was thought to play important function after fertilization in the mouse eggs.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.40 no.9
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• pp.1693-1698
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• 2015

This paper proposed a PFM(pulse-frequency modulator) boost converter which has dual pulse-width. The PFM boost converter is composed of BGR(band gap voltage reference generating circuit), voltage reference generating circuit, soft-start circuit, error amplifier, high-speed comparator, inductor current sensing circuit and pulse-width generator. Converter has different inductor peak current so it has wider load current range and smaller output voltage ripple. Proposed PFM boost converter generates 18V output voltage with input voltage of 3.7V and it has load current range of 0.1~300mA. Simulation results show 0.43% output voltage ripple at ligh load mode and 0.79% output voltage ripple at heavy load mode. Converter has efficiency 85% at light lode mode and it has maximum 86.4% at 20mA load current.

• Progress in Superconductivity and Cryogenics
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• v.6 no.3
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• pp.50-55
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• 2004

We fabricated and tested a resistive type superconducting fault current limiter (SFCL) of three-phase 6.6 $kV_{rms}/200 A_{rms}$ rating based on YBCO thin films grown on sapphire substrates with a diameter of 4 inches, Short circuit tests were carried out at a accredited test facility for single line-to- ground faults, phase-to-phase faults and three-phase faults, Each phase of the SFCL was composed of 8${\times}$6 elements connected in series and parallel respectively. Each element was designed to have the rated voltage of 600 $V_{rms}$. A NiCr shunt resistor of 23 Ω was connected to each element for simultaneous quenches. Firstly, single phase-to-ground fault tests were carried out. The SFCL successfully developed the impedance in the circuit within 0.12 msec after fault and controlled the fault current of 10 $kA_{rms} below 816 A_{peak}$ at the first half cycle. In addition, in case of phase-to-phase fault and three- phase fault test. simultaneous quenches among the SFCLs of the phases successfully accomplished. In conclusion. the SFCL showed excellent performance of current limitation upon fault and stable operation regardless of the amplitude of fault currents.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.12 no.4
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• pp.168-180
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• 2000

In the Part I, the three dimensional model testing with NNW deep water wave direction gave the results such that the occurrence of breaking waves over the peak of Ear-Do caused very small wave height at the structure position. But the measured wave forces were rather greater than the calculated forces based on deep water wave height. Furthermore, It was also perceived that the time series of the forces looked like corresponding to the case that waves were superimposed by an unidirectional current. In the present Part II, the current is presumed to be a flow secondly induced by breaking waves, and an extensive study to clarify the current in a quantitative sense is performed through numerical analysis and hydraulic experiment. The results showed that a strong circulation can surely occur in the vicinity of the structure due to radiation stress differentials given by the breaking waves. It was also recognized that the velocity of the induced current varied with the magnitude of energy dissipation rate introduced in the numerical analysis. The numerical analysis was tuned adjusting the dissipation rate so that the calculated wave field could closely match with the experimental results of Part I. The fluid force (in prototype) for the optimal match showed approximately 2.2% increased over the calculated value based on the deep water wave height (24.6m) whereas the force corresponding to the average of the experimental values showed the increase of about 13.0%.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.46-46
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• 2003

Salviae Miltiorrhizae Radix has been used for treatment of cardiovascular diseases in oriental medicine. To investigate the possible involvement of cardiac ion channel in this effect, we examined electrophysiological effects of the extract of Salviae Miltiorrhizae Radix on action potentials and ionic currents in rat ventricular myocytes. The extracts of Salviae Miltiorrhizae Radix were fractionated into nine fractions, and the effect of each fraction on action potential was tested. The fraction containing monomethyl lithospermic acid-A (LSA-A) induced a significant prolongation of action potential duration (APD). LSA-B which is a major component of Salviae Miltiorrhizae Radix, however, did not cause a significant effect. In voltage clamp experiments, the effects of LSA-A on K currents, Ca currents and Na currents were tested. Neither K currents nor L-type Ca currents were affected by LSA-A. On the contrary, LSA-A significantly slowed down the inactivation kinetics of the Na current with no effect on the fast component of the inactivation process. The amplitude of the peak current and the voltage-dependence of activation were not changed by LSA-A. The effect of LSA-A on Na current was abolished when high concentration of $Ca^{2+}$ buffer (10 mM BAPTA) was included in the pipette solution or when Ca2+ current was blocked by nicardipine (1 $\mu$M) in the bath solution.n.