• Title/Summary/Keyword: pattern transfer

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Expression of Bovine Growth Hormone Gene in a Baculovirus, Hyphantria cunea Nuclear Polyhedrosis Virus

  • Park, Kap-Ju;Lee, Keun-Kwang;Kang, Bong-Ju;Cha, Sung-Chul;Lee, Hyung-Hoan
    • The Journal of Korean Society of Virology
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    • v.28 no.2
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    • pp.129-138
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    • 1998
  • Bovine growth hormone (bGH) gene was expressed in an insect Spodoptera frugiperda cell line using a Baculovirus, Hyphantria cunea nuclear polyhedrosis virus (HcNPV). The bGH gene in pbGH plasmid was sequenced and amplified by PCR technique with two primers containing NcoI sites. The bGH gene consisted of 654 bp (217 amino acid residues), the 5'-untranslated region of the cloned bGH cDNA contains 56 bp, and the 3'-untranslated region contains 145 bp and two pallindromic regions. The amplified bGH gene DNA fragment (654 bp) was inserted into the NcoI site of the pHcEVII vector, which was named pHcbGH. The pHcbGH transfer vector DNA and the wild type HcNPV DNA were cotransfected into S. frugiperda cells to construct a recombinant virus. Eight recombinant viruses were selected and named HcbGH. One clone, HcbGH-4-1 showed largest plaque size, therefore the recombinant virus was further studied. The multiplication pattern of the recombinant HcbGH-4-1 was similar to that of the wild type HcNPV. The bGH gene DNA in the HcbGH-4-1 recombinant was confirmed by Southern blot hybridization. The amount of the bGH (217 amino acid residues, 21 kDa) produced in S. frugiperda cells infected with the HcbGH-4-1 recombinant was approximately 5.5 ng per ml ($10^6$ cells) by radioimmunoassay.

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Impact Shock Components and Attenuation in Flat Foot Running (편평족 달리기 시 충격 쇼크의 성분과 흡수)

  • Ryu, Ji-Seon;Lim, Ga-Young
    • Korean Journal of Applied Biomechanics
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    • v.25 no.3
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    • pp.283-291
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    • 2015
  • Objective : The purpose of this study was to determine the differences in the head and tibial acceleration signal magnitudes, and their powers and shock attenuations between flat-footed and normal-footed running. Methods : Ten flat-footed and ten normal-footed subjects ran barefoot on a treadmill with a force plate at 3.22m/s averaged from their preferred running speed using heel-toe running pattern while the head and tibial acceleration in the vertical axis data was collected. The accelerometers were sampled at 2000 Hz and voltage was set at 100 mv, respectively. The peak magnitudes of the head and tibial acceleration signals in time domain were calculated. The power spectral density(PSD) of each signal in the frequency domain was also calculated. In addition to that, shock attenuation was calculated by a transfer function of the head PSD relative to the tibia PSD. A one-way analysis of variance was used to determine the difference in time and frequency domain acceleration variables between the flat-footed and normal-footed groups running. Results : Peaks of the head and tibial acceleration signals were significantly greater during flat-footed group running than normal-footed group running(p<.05). PSDs of the tibial acceleration signal in the lower and higher frequency range were significantly greater during flat-footed running(p<.05), but PSDs of the head acceleration signal were not statistically different between the two groups. Flat-footed group running resulted in significantly greater shock attenuation for the higher frequency ranges compared with normal-footed group running(p<.05). Conclusion : The difference in impact shock magnitude and frequency content between flat-footed and normal-footed group during running suggested that the body had different ability to control impact shock from acceleration. It might be conjectured that flat-footed running was more vulnerable to potential injury than normal-footed running from an impact shock point of view.

Fabrication of Tungsten Nano Dot by Using Block Copolymer Thin Film (블록 공중합체 박막을 이용한 텅스텐 나노점의 형성)

  • Kang, Gil-Bum;Kim, Seong-Il;Kim, Yeung-Hwan;Park, Min-Chul;Kim, Yong-Tae;Lee, Chang-Woo
    • Journal of the Microelectronics and Packaging Society
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    • v.13 no.3 s.40
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    • pp.13-17
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    • 2006
  • Dense and periodic arrays of holes and tungsten none dots were fabricated on silicon oxide and silicon. The holes were approximately 25 nm wide, 40 nm deep, and 60 nm apart. To obtain nano-size patterns, self-assembling resists were used to produce layer of hexagonally ordered parallel cylinders of polymethylmethacrylate(PMMA) in polystyrene(PS) matrix. The PMMA cylinders were degraded and removed with acetic acid rinse to produce a PS mask for pattern transfer. The silicon oxide was removed by fluorine-based reactive ion etching(RIE). Selectively deposited tungsten nano dots were formed inside nano-sized trench by using a low pressure chemical vapor deposition(LPCVD) method. Tungsten nano dot and trenched silicon sizes were 26 nm and 30 nm, respectively.

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A Numerical Study on Coughed Particle Dispersion and Deposition in Negative Pressure Isolation Room according to Particle Size (음압격리병실에서의 기침 토출입자의 입경에 따른 확산 및 침적에 대한 수치해석 연구)

  • Jung, Minji;Hong, Jin Kwan
    • Journal of The Korea Institute of Healthcare Architecture
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    • v.24 no.2
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    • pp.37-44
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    • 2018
  • Purpose: This study investigates the influences of coughing direction and healthcare worker's location on the transport characteristics of coughed particles in airborne infection isolation room (AIIR), which is commonly called negative pressure isolation room, with a downward ventilation system. Methods: Computational Fluid Dynamics (CFD) was used to simulate the airflow and for tracing the behavior of particles. Results: The results show that the airflow pattern and coughing direction have a significant influence on the characteristics of particle dispersion and deposition. When healthcare workers are in the isolation room with the patient who is lying on the bed, it is recommended to be located far from the anteroom to reduce the exposures from infectious particles. And when the patient is lying, it is more effective in removing particles than when the patient is in Fowler's position. Although it is an isolation room that produces unidirectional flow, coughing particles can spread to the whole room and a large number of particles can be deposited onto patient, bed, side rails, healthcare worker, ceiling, floor, and sidewall. Implications: Following the patients' discharge or transfer, terminal cleaning of the vacated room, furniture, and all clinical equipment is essential. Also, it is necessary to establish detailed standard operating procedure (SOP) in order to reduce the risk of cross-contamination.

Influence of Changing Combustor Pressure on Combustion Characteristics and Local Reaction Intensity in the CH4/Air Flames (메탄/공기 화염에서 연소실 압력변동이 연소특성과 국소 반응강도에 미치는 영향)

  • Kim, Jong-Ryul;Choi, Gyung-Min;Kim, Duck-Jool
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.33 no.5
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    • pp.365-372
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    • 2009
  • The influence of combustor pressure on the local reaction characteristics of $CH_4$/air flames was investigated by measurements of local chemiluminescence intensity. Induced flow flames are often applied to the industrial boiler systems and incinerator in order to improve heat transfer and prevent exhaust gas leakage. In order to investigate combustion characteristics in the induced flow pattern, the combustor pressure index($P^*$) was controlled in the range of $0.7{\sim}1.3$ for each equivalence ratio in the present combustion system, where $P^*$ is defined as the ratio of absolute pressure to atmospheric one. Relationship between local reaction intensity and pressure index have been investigated by simultaneous $CH^*$, $C^*_2$ and $OH^*$ intensity measurements. It could be observed that flame length became longer with decreasing $P^*$ from $CH^*$ chemiluminescence intensity of axial direction. The mean value of $C^*_2$ and $CH^*$ chemiluminescence intensities, which indicates reaction intensity in the $CH_4$/air flames, decreased with decreasing pressure index for ${\Phi}{\leq}1$, but increased with decreasing pressure index for ${\Phi}$>1. $C^*_2/CH^*$ intensity ratio, which can be a good marker to demonstrate local equivalence ratio, was almost same for ${\Phi}{\leq}1$ regardless of pressure index change, while they showed high level for lower pressure index for ${\Phi}$>1 conditions.

An Adaptive Region-of-Interest Coding Based on EBCOT (EBCOT 기반의 적응적 관심영역 코딩)

  • Kang, Ki-Jun;Lee, Bu-Kwon;Seo, Yeong-Geon
    • Journal of Korea Multimedia Society
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    • v.9 no.11
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    • pp.1445-1454
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    • 2006
  • To compress a specific part of an image with high quality or to transfer it, JPEG2000 standard offers an ROI(Region-of-Interest) image coding method. What is important in ROI coding is to process relative importance between ROI and background and to process ROI mask. We propose an adaptive ROI coding method supplemented the existing Implicit ROI coding and Modified implicit ROI coding to improve image quality and reduce ROI mask information. The proposed method is an EBCOT-based ROI coding that extracts ROI from the compressed bitstream, and gets the ROI mask information by classifying the codeblocks into 6 patterns. The information includes the pattern type(3bit) and the width(5bit) expressing the boundary between two regions for each codeblock. As a result, the method shows an excellent compression performance in ROI region as well as in the whole region of an image.

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Self-Configuration System based on Context Adaptiveness (상황적응기능기반 자가구성 시스템)

  • Lee, Seung-Hwa;Lee, Eun-Seok
    • The KIPS Transactions:PartD
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    • v.12D no.4 s.100
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    • pp.647-656
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    • 2005
  • This paper proposes an adaptive resource self-management system that collects system resources, user information, and usage patterns as context information for utilization in self-configuration. This system ill ease the system maintenance burden on users by automation of large part of configuration tasks such as install, reconfiguration and update, and will also decrease cost and errors. Working from the gathered context information, this system allows users to select appropriate components and install them for user's system context. This also offers a more personalized configuration setting by using user's existing application setting and usage pattern. To avoid the overload on central server to transfer and manage related files, we employ Peer-to-Peer method. h prototype was developed to evaluate the system and a comparison study with the conventional methods of manual configuration and MS-IBM systems was conducted to validate the proposed system in terms of functional capacity, install time and etc.

Bad Faith Intent in Internet Address Resources Act (인터넷주소자원에 관한 법률 제12조에 규정된 부정한 목적의 해석 : 대법원 2013. 4. 26. 선고 2011다64836 판결을 중심으로)

  • Park, Young-Gyu
    • Journal of Information Technology Services
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    • v.13 no.3
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    • pp.129-148
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    • 2014
  • Generally, the Internet Address Resources Act is intended to protect the public from acts of Internet "cybersquatting", a term used to describe the bad faith, abusive registration of Internet domain names. In determining whether a person has a bad faith intent, a court may consider factors such as, (1) the trademark or other intellectual property rights of the person, if any, in the domain name, (2) the extent to which the domain name consists of the legal name of the person or a name that is otherwise commonly used to identify that person, (3) the person's prior use, if any, of the domain name in connection with the bona fide offering of any goods or services, (4) the person's bona fide noncommercial or fair use of the mark in a site accessible under the domain name, (5) the person's intent to divert consumers from the mark owner's online location to a site accessible under the domain name that could harm the goodwill represented by the mark, either for commercial gain or with the intent to tarnish or disparage the mark, by creating a likelihood of confusion as to the source, sponsorship, affiliation, or endorsement of the site, (6) the person's offer to transfer, sell, or otherwise assign the domain name to the mark owner or any third party for financial gain without having used, or having an intent to use, the domain name in the bona fide offering of any goods or services, or the person's prior conduct indicating a pattern of such conduct.

Design of E-Tongue System using Neural Network (신경회로망을 이용한 휴대용 전자 혀 시스템의 설계)

  • Jung, Young-Chang;Kim, Dong-Jin;Kim, Jeong-Do;Jung, Woo-Suk
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.6 no.2
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    • pp.149-158
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    • 2005
  • In this paper, we have designed and implemented a portable e-tongue (electronic tongue) system using MACS (multi array chemical sensor) and PDA. The system embedded in PDA has merits such as comfortable user interface and data transfer by internet from on-site to remote computer. MACS was made up 7 electrodes (${NH_4}^+$, $Na^+$, $Cl^-$, ${NO_3}^-$, $K^+$, $Ca^{2+}$, $Na^+$, pH) and a reference electrode. For learning the system, we adapted the Levenberg-Marquardt algorithm based on the back-propagation, which could iteratively learned the pre-determined standard patterns, in e-tongue system. Conclusionally, the relationship between the standard patterns and unknown pattern can be easily analyzed. The e-tongue was applied to whiskeys and cognac (one high level whisky, one low level whiskey, two cognac) and 2 sample whiskeys for each standard patterns and unknown patterns. The relationship between the standard patterns and unknown patterns can be easily analyzed.

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Dynamic Control of Random Constant Spreading Worm using Depth Distribution Characteristics

  • No, Byung-Gyu;Park, Doo-Soon;Hong, Min;Lee, Hwa-Min;Park, Yoon-Sok
    • Journal of Information Processing Systems
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    • v.5 no.1
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    • pp.33-40
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    • 2009
  • Ever since the network-based malicious code commonly known as a 'worm' surfaced in the early part of the 1980's, its prevalence has grown more and more. The RCS (Random Constant Spreading) worm has become a dominant, malicious virus in recent computer networking circles. The worm retards the availability of an overall network by exhausting resources such as CPU capacity, network peripherals and transfer bandwidth, causing damage to an uninfected system as well as an infected system. The generation and spreading cycle of these worms progress rapidly. The existing studies to counter malicious code have studied the Microscopic Model for detecting worm generation based on some specific pattern or sign of attack, thus preventing its spread by countering the worm directly on detection. However, due to zero-day threat actualization, rapid spreading of the RCS worm and reduction of survival time, securing a security model to ensure the survivability of the network became an urgent problem that the existing solution-oriented security measures did not address. This paper analyzes the recently studied efficient dynamic network. Essentially, this paper suggests a model that dynamically controls the RCS worm using the characteristics of Power-Law and depth distribution of the delivery node, which is commonly seen in preferential growth networks. Moreover, we suggest a model that dynamically controls the spread of the worm using information about the depth distribution of delivery. We also verified via simulation that the load for each node was minimized at an optimal depth to effectively restrain the spread of the worm.