• Title/Summary/Keyword: pattern transfer

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Development of Bovine Embryos Produced by Intracytoplasmic Sperm Injection (ICSI)

  • Ock, S.A.;Kwack, D.O.;Cho, S.R.;Cho, S.K.;Yeao, E.H.;Yoo, J.G.;Lee, Y.R.;Lee, H.J.;Choe, S.Y.;Rho, G.J.
    • Journal of Embryo Transfer
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    • v.17 no.1
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    • pp.13-21
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    • 2002
  • Intracytoplasmic Sperm Injection (ICSI) has been widely used fur both human infertility and basic research. However, the high incidence of chromosomal abnormality is severe problem in cattle. Various oocyte activation stimuli, therefore, were compared by assessment of developmental capacity and chromosome analysis. Motile sperm selected by Percoll-density gradient were treated with 5 mM dithiothreitol (DTT) and injected into an oocyte matured fur 24 h. Eggs were then allocated into 5 treatment groups. Group 1 (control), sperm injection was performed without any further activation stimuli to the oocytes. Group 2 (handled control), sham injection was performed without sperm. In Group 3, oocytes exposed to 5 (M ionomycin for 5 min at 39(C. Group 4. ionomycine + 1.9 mM demethylaminopurine (DMAP, 3 h) and Group 5, ionomycine + 3 h culture in Ml99 + DMAP. Cleavage and the later development rate in Groups 1, 2 and 3 were significantly (P<0.05) lower than those in Groups 4 and 5. The incidence of chromosomal abnormality in the embryos treated directly with DMAP after ionomycine was relatively higher than in the embryo of Group 3 h, delayed DMAP treatment. From this results DMAP caused to be arrested the release of the 2nd polar body, resulting in changes of chromosomal pattern. Therefore, the time interval between ionomycin and DMAP is a crucial role in bovine ICSI.

Natural Convection Heat Transfer on Inclined Plates (경사진 평판에서의 자연대류 열전달)

  • Lim, Chul-Kyu;Heo, Jeong-Hwan;Chung, Bum-Jin
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.35 no.7
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    • pp.701-708
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    • 2011
  • Natural convection heat transfers on inclined flat plates were measured for Grashof numbers of $8.06{\times}10^7$ and $3.45{\times}10^9$ by using a copper sulfate electroplating system. The inclinations of the plates were varied from upward-facing horizontal to downward-facing horizontal. Test results for the downward-facing plate agree well with the existing theory that the Nusselt number can be calculated by replacing gravitational acceleration, g with g $cos{\theta}$ in the heat transfer correlation for the vertical plate. The natural convection flows for the upward-facing plate follow two distinct flow regimes: boundary layer regime and flow separation regime. The copper plating pattern for the upward-facing plates clearly reveals the flow separation points.

Categorization of two different swing styles using weight transfer patterns of golf swing (압력 중심 및 지면반력 이동 패턴에 의한 두 가지의 다른 골프 스윙 스타일 분류)

  • Linm, Young-Tae
    • Korean Journal of Applied Biomechanics
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    • v.14 no.2
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    • pp.179-186
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    • 2004
  • 본 연구의 목적은 숙련된 골프선수들의 골프 스윙동작 시 상이한 무게중심 변환 형태가 존재하는지 그리고 만약 이 상이한 무게중심 변환 형태가 존재한다면 어떤 형태로 나타나는지를 알아보고자 하였다. 본 실험을 위해 13명의 남자 대학 엘리트 골프 선수들을 대상으로 드라이버를 이용한 스윙을 실시하게 하였다. 수직 지면 반력과 압력중심 이동 패턴을 측정하기 위해 지면반력기 2대와 스윙 동작 시 critical event 설정을 위해 1대의 고감도 비디오 카메라를 사용하였다. 또한 스윙 결과로 나타나는 비거리, 클럽헤드 속도를 측정하기 위해 스윙 분석기를 이용하였다. 피험자 간 상호 비교를 위해 측정된 수직 지면반력 성분은 몸무게를 이용하여 표준화하였고 압력중심은 어드레스시의 압력중심을 원점으로 재 계산하였다. 또한 임팩트 시 측정된 좌우 수직 지면반력 성분을 이용하여 두 가지의 상이한 스윙 스타일로 구분하였다. 연구결과 8명(62%)의 피험자에게서 좌측 수직력 성분이 상대적으로 높은 전족(FFS) 스타일임이 밝혀졌고 5명(38%)은 그 반대인 리버스(RFS) 스타일임이 밝혀졌다. 이 전족 스타일은 대부분의 골퍼들이 선호하고 또 그렇게 하여야만 한다고 믿고 있는 스윙방식이다. 이와 반대인 리버스 스타일은 코치들이 금기시 하는 스윙의 형태로서 초보자, 즉 비숙련 자에게만 나타나는 전형적인 스윙형태라고 인지되고 있다. 하지만 이 두 가지 스윙 스타일이 초보자가 아닌 숙련된 골프 선수들에게서도 실제 존재한다는 것이 본 실험 결과로 나타났다. 평균 압력중심 이동 패턴은 전후축 방향의 경우 RFS 스타일 그룹이 RFS 스타일 그룹에 비해 압력중심 이동이 크게 나타났고 좌우축의 경우에도 RFS 그룹이 FFS 그룹에 비해 압력중심 이동이 크게 나타났다. 특히 임팩트 시 압력중심의 위치가 FFS 그룹은 전족 근처에 있는 반면 RFS 그룹은 후족(rear foot)에 위치하여 두 스윙 스타일의 차이점을 분명하게 보여주었다. 따라서 비록 이 RFS 스타일이 대부분의 코칭 이론과는 상반되는 무게중심 이동 패턴을 보여주었지만 이 스윙 스타일이 잘못된 것이 아닌 실제 존재하는 하나의 스윙 스타일로 인식하여 스윙 지도 시 이에 알맞은 적절한 교습법을 마련해야 할 것이다.

Data Mining Analysis of Educational and Research Achievements of Korean Universities Using Public Open Data Services (정보공시 자료를 이용한 교육/연구성과 영향요인 추출 및 대학의 군집 분석)

  • Shin, Sun Mi;Kim, Hyeon Cheol
    • The Journal of Korean Association of Computer Education
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    • v.17 no.1
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    • pp.117-130
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    • 2014
  • The purpose of this study is to provide useful knowledge for improving indicators that represent competitiveness and educational competency of the university by deriving a new pattern or the meaningful results from the data of information disclosure of universities using statistical analysis and data mining techniques. To achieve this, a model of decision tree was made and various factors that affect education/research performance such as employment rate, the number of technology transfer and papers per full-time faculty were explored. In addition to this, the cluster analysis of universities was conducted using attributes related to evaluation of university. According to the analysis, common factors affecting higher education/research performance are following indicators ; incoming student recruitment rate, enrollment rate, and the number of students per full-time faculty. In the cluster analysis, when performed by the entire university, the size, location of the university respectively, clusters are mainly formed by well-known universities, art physical non-science and engineering religious leaders training universities, and others. The main influencing factors of this cluster are higher education/research performance indicators such as employment rate and the number of technology transfer.

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A VLSI Pulse-mode Digital Multilayer Neural Network for Pattern Classification : Architecture and Computational Behaviors (패턴인식용 VLSI 펄스형 디지탈 다계층 신경망의 구조및 동작 특성)

  • Kim, Young-Chul;Lee, Gyu-Sang
    • Journal of the Korean Institute of Telematics and Electronics B
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    • v.33B no.1
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    • pp.144-152
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    • 1996
  • In this paper, a pulse-mode digital multilayer neural network with a massively parallel yet compact and flexible network architecture is presented. Algebraicneural operations are replaced by stochastic processes using pseudo-random pulse sequences and simple logic gates are used as basic computing elements. The distributions of the results from the stochastic processes are approximated using the hypergeometric distribution. A statistical model of the noise(error) is developed to estimate the relative accuracy associated with stochastic computing in terms of mean and variance. Numerical character recognition problems are applied to the network to evaluate the network performance and to justify the validity of analytic results based on the developed statistical model. The network architectures are modeled in VHDL using the mixed descriptions of gate-level and register transfer level (RTL). Experiments show that the statistical model successfully predicts the accuracy of the operations performed in the network and that the character classification rate of the network is competitive to that of ordinary Back-Propagation networks.

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A Flexible Operation Plan of Free Ride System Based on Transport Card Data in Seoul Metropolitan Area (수도권 교통카드자료를 활용한 무임승차제도의 탄력적 운영방안)

  • Lee, Chang Hun;Kim, Sigon;Yun, Gyeong Cheol
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.36 no.6
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    • pp.1069-1073
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    • 2016
  • The current free-riding subway system for the elderly over 65 years old has been implemented since in 1980. As the aging population increases, the number of free riders also increases. It results eventually in the increase of subsidy from government. In addition, a transfer between subway and bus is not good enough since the free ride system is applied to only subway not to bus. The aim of this study is to analyze the transport card data to understand the impact of free-riding in the viewpoint of economic issues. It aims also to analyze the transfer patterns between public transport. At the end it compares Korea case with international cases and finds out some issues on free-ride systems. Finally, counter-measures aimed at improving the current free-rider system is suggested.

Variation of Transcribed X-linked Genes in Bovine Embryos Cloned with Fibroblasts at Different Age and Cell Cycle

  • Jeon, Byeong-Gyun;Rho, Gyu-Jin
    • Reproductive and Developmental Biology
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    • v.35 no.2
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    • pp.175-183
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    • 2011
  • The present study compared the developmental potential, telomerase activity and transcript levels of X-linked genes (ANT3, HPRT, MeCP2, RPS4X, XIAP, XIST and ZFX) in the bovine somatic cell nuclear transfer (SCNT) embryos derived from different age and cell cycle of female donor nucleus. In experiment 1, the fusion rate, cleavage rate to 2-cell stage, developmental rate to blastocyst stage, and the mean number of total and ICM cells was slightly increased in embryos cloned with fetal fibroblasts compared to those with adult fibroblasts, but there was no significantly (p<0.05) differences. Telomerase activity was also similar in blastocysts cloned with fetal and adult fibroblasts. Up-regulated RPS4X and down-regulated MeCP2, XIAP, and XIST transcript level were observed in blastocysts cloned with adult fibroblasts, compared to those with fetal fibroblasts. In experiment 2, the fusion rate, cleavage rate to 2-cell stage, developmental rate to blastocyst stage, and the mean number of total and ICM cells was significantly (p<0.05) increased in embryos cloned with fetal fibroblasts at early G1 phase of the cell cycle, compared to those of fetal fibroblasts at late G1 phase. DNMT1 transcript was observed to significantly (p<0.05) increased in the fetal fibroblasts at 3 hrs after trypsin treatment of confluent culture. Further, level of telomerase activity and transcribed X-linked genes was also significantly (p<0.05) higher in the early G1 SCNT blastocysts than those of late G1. The results imply that fetal fibroblasts at early G1 phase induces the enhanced developmental potential and up-regulated telomerase activity and X-linked gene, but aberrant transcript pattern of X-linked genes may be displayed in the SCNT embryos.

A Biomechanical Analysis of Stress Transfer Behaviors Within the Necrotic Area of Femoral Head secondary to Changes in Core Placement Direction on Various Distributions of Necrotic Areas in the Osteonecrosis of the Femoral Head (대퇴골두 무혈성 괴사증에 있어서 괴사 영역의 위치와 천공방향의 변화에 따른 대퇴골두 괴사영역에서의 응력 변화 분석에 대한 생체역학적인 고찰)

  • Lim, D.H.;Lee, S.J.;Kim, J.S.;Shin, J.W.;Kim, Y.S.
    • Proceedings of the KOSOMBE Conference
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    • v.1998 no.11
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    • pp.157-158
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    • 1998
  • The purpose of this study was to test the hypothesis that even very small change of the cue direction in the treatment of the early osteonecrosis could affect the outcomes of operation. For this, the changes in stress transfer within the necrotic area of the femoral head were investigated under various directions and placements of the core utilizing finite element method. The loading of 3188N, which represents after-heel-strike, was imposed in cubic cosine pattern. All nodes on the most distal surface of the model were constrained in all directions. All materials included were assumed to have linear-elastic behavior. The result says that the critical stress, which causes collapse of the femoral head, was reduced when the core was oriented toward the posterior side of the femoral head regardless of location of the necrotic area. The same result was obtained either fibular bone grafting or cementation was adopted. As a consequence, the biomechanical study suggests that the core should be directed toward the loading point where the resultant force is applied to get more desirable treatment of the osteonecrosis of the femoral head in the early stage.

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Study on Smart Cooling Technology by Acoustic Streaming Generated by Ultrasonic Vibration Using 3D PIV (3차원 PIV를 활용한 초음파 진동에 의해 발생된 음향 유동을 이용한 스마트 냉각법 연구)

  • Lee, Dong-Ryul;Loh, Byoung-Gook;Kwon, Ki-Jung
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.20 no.11
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    • pp.1078-1088
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    • 2010
  • In order to analyze the quantitative characteristics of acoustic streaming, experimental setup of 3-D stereoscopic PIV(particle imaging velocimetry) was designed and quantitative ultrasonic flow fields in the gap between the ultrasonic vibrator and heat source were measured. Utilizing acoustic streaming induced by ultrasonic vibration, surface temperature drop of cooling object was also measured. The study on smart cooling method by acoustic streaming induced by ultrasonic vibration was performed due to the empirical relations of flow pattern, average flow velocity, different gaps, and enhancement on cooling rates in the gap. Average velocity fields and maximum acoustic streaming velocity in the open gap between the stationary cylindrical heat source and ultrasonic vibrator were experimentally measured at no vibration, resonance, and non-resonance. It was clearly observed that the enhancement of cooling rates existed owing to the acoustic air flow in the gap at resonance and non-resonance induced by ultrasonic vibration. The ultrasonic wave propagating into air in the gap creates steady-state secondary eddy called acoustic streaming which enhances heat transfer from the heat source to encompassing air. The intensity of the acoustic streaming induced by ultrasonic vibration experimentally depended upon the gap between the heat source and ultrasonic vibrator. The ultrasonic vibration at resonance caused the increase of the acoustic streaming velocity and convective heat transfer augmentation when the flow fields by 3D stereoscopic PIV and temperature drop of the heat source were measured experimentally. The acoustic streaming velocity of air enhancement on cooling rates in the gap is maximal when the gap agrees with the multiples of half wavelength of the ultrasonic wave, which is specifically 12 mm.

Studies on Development of Breeding Technique to Increase Hanwoo(Bos taurus coreanae) I. Survey of Reproductive Status and Effect of Intraovarian $\textrm{PGF}_{2a}$ Administration on Luteolysis and Subsequent Estrus Induction (한우의 신속한 증식을 위한 번식기술 개발에 관한 연구 I. 한우 번식실태 및 $\textrm{PGF}_{2a}$의 난소실질 내 투여효과)

  • 이병천
    • Journal of Embryo Transfer
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    • v.15 no.1
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    • pp.77-83
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    • 2000
  • The purpose of this study was to develop a breeding technique to increase Hanwoo of superior characteristics. In the present study, reproductive status of Hanwoo such as size of farm, breeding system and gestationi length was investigated. In addition, effect of low dose administration of prostaglandin F2$\alpha$(PGF2$\alpha$) on luteolysis was examined. The size of farm was classified by the total number of cows and the number of breeding stocks, respectively. The distribution of herd size of < 5, 6~10, 11~30, 31~50 and > heads was 31%, 15%, 39%, 4% and 11%, respectively. Furthermore, the distribution of breeding stock size of <5, 6~10, 11~30, 31~50 and > 50 heads was 36%, 28%, 31%, 3% and 3%, respectively. Average parity was 2.1 in breeding stock. In breeding pattern, artificial in semination(A.I), estrus synchronization-A.I and natural mating was 92.7%, 2.4% and 4.9% respectively. Gestational length of Hanwoo was ranged 253~316 days (average length : 285 days) after estrus( estrus=0). To induce luteolysis, PGF2$\alpha$ was injected into ovarian parenchyma by a modified ovarian injector. The effect of administration of 6mg PGF2$\alpha$ on luteolysis and estrus induction was betweer (P<0.01) when PGF2$\alpha$ was administered into ovarian parechyma than when administered intramuscluarly (71 vs. 91%). When PGF2$\alpha$ was injected into ovarian parenchyma, a decreased concentration to 3 mg did not significantly decreaed its luteolytic effect(92%). When AI was performed following PGF2$\alpha$ treatment, the intraovarian injection group yielded a higher pregnancy rate(69 vs. 88%) than the IM injection groups, regardless of the dosage. In conclusion these results suggest that increasing herd size and regular reproductive management are needed to improve reproductive efficiency in Hanwoo industry. Furthermore, intraovarian administration of PGF2$\alpha$ is effective way to induce luteolysis compared with intramuscular injection.

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