• Title/Summary/Keyword: pattern transfer

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Construction of Interspecific Hybrids detween Aspergillus spp. by Nuclear transfer (수종의 Aspergillus 속 균 사이의 핵전이에 의한 종간잡종 형성)

  • 노형선;이정애;이영하;김진미;정재훈;맹필재
    • Korean Journal of Microbiology
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    • v.29 no.1
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    • pp.8-15
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    • 1991
  • Interspecific hybrids between the ASpergillus spp., A. awamori, A. usamii and A. oryzae, were obtained by nuclear transfer technique. Nuclei isolated from an auxotrophic mutant strain were transferred into the protoplasts of a recipient strain of different species. The frequency of interspecific hybrid formation by nuclear transfer was $2*10^{-5}$ $-7*10^{-4}$ In contrast, no interspecific hybrid was isolated by protoplast fusion. Among the hybrids tested, 10 strains showed increased activity of some or all components of cellulases, xylanases and amylase up to more than two times. Isozyme pattern of the hybrids were analyzed by polyacrylamide gel electrophoresis and isoelectric focusing followed by activity staining, which showed that some of the hybrids have isozyme patterns unidentical to either of the two parents. By measuring the DNA contents and the sizes ofthe conidia, the karyotypes of the hybrids were estimated to be aneuploid near to haploid, diploid or triploid. It was concluded that the unclear transfer technique is much more efficient in the formation of interspecific hybrids than protoplast fusion and is very useful for the improvement of Aspergillus strains.

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An Evaluation of Heat Transfer Characteristics of Individualized Fin Type Using Large Scale Model (확대모델을 이용한 특성화된 핀형상의 열전달평가)

  • 윤점열;강희찬;이관수;김무환
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.8 no.1
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    • pp.166-175
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    • 1996
  • In recent years, air-conditioning units designed with higher efficiency and more compactness are required due to emphasis on energy efficiency in home electrical appliances. This trend in the air-conditioning industries has accelerated the development of improved heat exchanger with a better performance in heat transfer aspects. In this study, experiments were performed to investigate the shape and configuration of fins affecting on the performance of the conventional fin-and-tube type heat exchanger equipped in a commercial air conditioners. The geometry similtude method was employed to measure the heat transfer coefficient and pressure drop. Experimental results show that this method is very useful to analyze the heat transfer characteristics of the fin-and-tube type heat exchanger. It is also found that the slit fin has better performance than the conventional fin type in the air conditioners. The present results indicate that heat transfer from the fin si influenced by the parameters such as the forming area of the slit fin, the type of interrupted surfaces like a louver or slit, slit patterns and slit raised direction, and it also affects the overall heat exchanger performance.

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A Korean Family with Cholesterol Ester Transfer Protein Deficiency

  • Youn, Seo-Young;Shin, Ik-Soon;Hong, Yong-Hee;Lee, Dong-Hwan
    • Journal of Genetic Medicine
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    • v.9 no.1
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    • pp.38-41
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    • 2012
  • A 32-year-old female patient and her sister show high levels of high density lipoprotein (HDL) cholesterol in regular health checkups, since female patient was 11 years old. The patient's serum total cholesterol was 285 mg/dL and HDL cholesterol was 113 mg/dL. Her sister's total cholesterol was 240 mg/dL and the HDL cholesterol measured to be 90 mg/dL. Lipoprotein pattern and cholesteryl ester transfer activity gene analysis were examined in these patients. We found c.1321+1G>A (IVS14+1G/A) hetero mutation in cholesteryl ester transfer protein (CETP) genes. Generally, CETP mediates transfer and exchange of triglycerides and cholesteryl ester between plasma lipoproteins. Also we investigated a key role of HDL-CE and Apo A-1 metabolism. Patients with low levels of CETP have increased serum HDL levels. We hereby report two Korean cases of CETP deficiency in a family. Brief literature review ensues with the cases.

A Study on the Design of Index Table Drive of Rotary Transfer Machines to Reduce Cycle Time (사이클 타임 단축을 위한 로터리 트랜스퍼 머신의 인덱스 테이블 구동부 설계에 관한 연구)

  • Huh, Ki-Seok;Park, Yong-Woo;Kim, Dong-Seon;Lyu, Sung-Ki
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.21 no.8
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    • pp.60-65
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    • 2022
  • This study focuses on the driving control design of an index, which is a key component of a rotary transfer machine that is effective in improving productivity and reducing manufacturing costs by shortening cycle time. Although various index studies have been conducted on the rotation of workpieces such as general-purpose machine tools and tilting indices, the development of an index for rotary transfer machines for transfer is insufficient. The index consists of a body, table, hydraulic cylinder, motor, reducer, and curved coupling. The torque of the table for driving was selected, and the angular velocity and torque pattern were simulated using the motor manufacturer's program. The specifications of the drive motor were determined based on the selected torque.

Heat Transfer in Rotating Duct with $70^{\circ}$ Angled Ribs (회전하는 덕트내 설치된 $70^{\circ}$ 경사요철의 열전달 특성)

  • Choi, Chung;Lee, Sei Young;Won, Jung Ho;Cho, Hyung Hee;Park, Byung kyu
    • The KSFM Journal of Fluid Machinery
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    • v.4 no.3 s.12
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    • pp.7-13
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    • 2001
  • The present study investigates convective heat/mass transfer and flow characteristics inside a cooling passage of rotating gas-turbine blades. The rotating duct has staggered ribs with $70^{\circ}$ attack angle, which are attached on leading and trailing surfaces. Naphthalene sublimation technique is employed to determine detailed local heat transfer coefficients using the heat and mass transfer analogy. Additional numerical calculations are conducted to analyze the flow patterns in the cooling passage. The present experiments employ two-surface heating conditions in the rotating duct because the exposed surfaces to hot gas stream are pressure and suction side surfaces in the middle passages of an actual gas-turbine blade. Secondary flows are generated by Coriolis and centrifugal forces in the spanwise and streamwise directions. The ribs attached on the walls disturb the mainflow resulting in recirculation and secondary flows near the ribbed wall. The local heat transfer and flow patterns in the passage are changed significantly according to rib configurations and duct rotation speeds. Therefore, the geometry and arrangement of the ribs are important for the advantageous cooling performance. The experimental results show that the ribs enhance the heat transfer more than $70\%$ from that of the smooth duct. The duct rotation generates the heat transfer discrepancy between the leading and trailing walls due to the secondary flows induced by the Coriolis force. The overal heat transfer pattern on the leading and trailing walls for the first and second passes are depended on the rotating speed, but the local heat transfer trend is affected mainly by the rib arrangements.

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Transfer Patterns of Multiple Trauma Patients in University Hospital after Acute Phase Management (대학병원에서 급성기 치료가 완료된 다발성 외상환자의 전원 패턴)

  • Lee, Jong Min;Jang, Ji Young;Lee, Seung Hwan;Lee, Jae Gil
    • Journal of Trauma and Injury
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    • v.26 no.4
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    • pp.261-265
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    • 2013
  • Purpose: The aim of this study is to evaluate the transfer pattern of multiple trauma patients after acute phase management and to determine whether the time between the surgeon's decision and the actual transfer correlates with the patient's insurance type. Methods: Three hundred ninety-two(392) multiple trauma patients visited the emergency room from January 2011 to April 2013. Among the 143 patients who were admitted by a trauma surgeon, 47 were transferred to another hospital after acute phase management. The age, gender, trauma mechanism, Revised trauma score (RTS), Injury severity score (ISS), insurance type, length of ICU stay and hospital stay were analyzed through a retrospective chart review. Results: The mean age was 47.7 years, and traffic accident was the most common mechanism(26, 55.3%). The mean RTS and ISS were 6.93 and 22.7, respectively. Twenty-five patients(53%) were covered by National health insurance, and 20 patients(42.6%) were covered by automobile insurance. Patients were transferred to primary (4.3%), secondary(80.9%), tertiary(4.3%) and care(10.6%) hospitals. The mean time from transfer decision to actual transfer was significantly longer for patients who were covered by automobile insurance than it was for patients who were covered by national health insurance (p=0.038). Conclusion: An appropriate transfer system at the end of acute phase care is essential for managing trauma centers with limited staffing and facilities. In addition, the mean time from transfer decision to actual transfer seemed to be definitely related to the type of insurance covering the patient.

Development of the Large-area Au/Pd Transfer-printing Process Applying Both the Anti-Adhesion and Adhesion Layers (접착방지막과 접착막을 동시에 적용한 대면적 Au/Pd 트랜스퍼 프린팅 공정 개발)

  • Cha, Nam-Goo
    • Korean Journal of Materials Research
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    • v.19 no.8
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    • pp.437-442
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    • 2009
  • This paper describes an improved strategy for controlling the adhesion force using both the antiadhesion and adhesion layers for a successful large-area transfer process. An MPTMS (3-mercaptopropyltrimethoxysilane) monolayer as an adhesion layer for Au/Pd thin films was deposited on Si substrates by vapor self assembly monolayer (VSAM) method. Contact angle, surface energy, film thickness, friction force, and roughness were considered for finding the optimized conditions. The sputtered Au/Pd ($\sim$17 nm) layer on the PDMS stamp without the anti-adhesion layer showed poor transfer results due to the high adhesion between sputtered Au/Pd and PDMS. In order to reduce the adhesion between Au/Pd and PDMS, an anti-adhesion monolayer was coated on the PDMS stamp using FOTS (perfluorooctyltrichlorosilane) after $O_2$ plasma treatment. The transfer process with the anti-adhesion layer gave good transfer results over a large area (20 mm $\times$ 20 mm) without pattern loss or distortion. To investigate the applied pressure effect, the PDMS stamp was sandwiched after 90$^{\circ}$ rotation on the MPTMS-coated patterned Si substrate with 1-${\mu}m$ depth. The sputtered Au/Pd was transferred onto the contact area, making square metal patterns on the top of the patterned Si structures. Applying low pressure helped to remove voids and to make conformal contact; however, high pressure yielded irregular transfer results due to PDMS stamp deformation. One of key parameters to success of this transfer process is the controllability of the adhesion force between the stamp and the target substrate. This technique offers high reliability during the transfer process, which suggests a potential building method for future functional structures.

The Guidelines for Station Layouts and Their Assessments in Railroad Transfer Centers (철도 환승센터의 시설배치기준 및 평가방안)

  • Lee, Jong kuk;Kim, Sigon;Lim, Kwangkyun
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.29 no.5D
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    • pp.597-604
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    • 2009
  • The mass transit networks in Korea, such as buses, metro and subway, are co-related and the number of transfer points, in which a lot of pedestrian movement is generated, are rising. The functions of the transfer centers, in which several travel modes cross at a point, have to include convenience and comfort for pedestrians using the transfer centers, and such functions can make transit a more attractive travel option and thus eventually contribute to increases in ridership. However, the guidelines for station layouts and their assessments in transfer stations are not suggested so far. So the pedestrian pattern data (pedestrian walking distances and times, pedestrian approaching distances and times, pedestrian walking paths in the inside of stations) was collected and analyzed. Based on the results of the analysis, the ways to measure the quality of comfort for those who using transfer facilities were suggested using the times that pedestrians consume to transfer from a travel mode to another. The walking time of pedestrians at the inside of stations was also measured by pre-selected pedestrian pathways as the guidelines for station layout assessment in transfer facilities.

Experimental measurements of R-22 two-phase friction factor in plate heat exchangers (판형열교환기에서 R-22 냉매의 이상 압력 손실계수 평가)

  • Yoo, Sang-Roon;Jeong, Ji-Rwan
    • Proceedings of the KSME Conference
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    • 2007.05b
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    • pp.2273-2278
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    • 2007
  • Brazed Plate Heat Exchanger (BPHE) is a type of compact plate heat exchanger with parallel corrugated plates which are brazed together in series. Each plate hascorrugation called herringbone pattern. Inside a BPHE, hot fluid and cold fluid alternate its flow direction to establish counter current flow configuration. Two-phase flow heat transfer and pressure drop of R-22 in BPHE were experimentally measured in this study. In the present experiments, single-phase region and two-phase region coexist in a BPHE. Therefore, the inside of a BPHE have to be divided into single phase region and two phase region and analyzed accordingly. The results from the single phase flow analysis are then extended to the two phase flow analysis to correlate the condensation and evaporation heat transfer and pressure drop for the refrigerant R-22 in the BPHEs. Previous models for two- phase friction factor have been compared with the present experimental results.

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Investigation of load transfer along interfaces of jacketed square columns

  • Achillopoulou, Dimitra V.
    • Structural Engineering and Mechanics
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    • v.63 no.3
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    • pp.293-302
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    • 2017
  • This study deals with a numerical investigation of load transfer along interfaces of jacketed columns using finite element models. Appropriate plasticity and constitutive models are used to simulate the response of concrete and steel bars. Experimental data were used to calibrate the simulation of mechanical characteristics. The different compressive strength of core and jacket concrete, the confinement ratio, the dowels' diameter size and the load pattern shapes were considered. The path diagrams along the interfaces elucidate the areas around the dowel bars where due to stress concentration plastic hinges and intense discontinuities are created. The stress flow also depicts the contribution of confinement of the jacketed area to the overall resonant load capacity of the core column. The scope of the research is to identify and quantify the shear transfer along the interfaces of strengthened elements.