• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.195.2-196
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• 2003

Cinnamic acid derivatives (CAD) originating from medicinal plants have some biological activity. In this study. effect of CAD on heterologous passive cutaneous anaphylaxis (PCA) were studied by the method of Levine and Vaz(1970). Anti-serum was prepared from OA-sensitized male Balb/c mouse at two weeks after the last challenge of ovalbumin (OA) and alumina gel, and these serum diluted with HEPES buffer by means of the heterologous PCA titer, i.e. the highest dilution inducing PCA in rats. (omitted)

• 한국식품과학회지
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• 제40권5호
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• pp.568-573
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• 2008

난백의 알레르겐을 감소시키기 위해서 여러 가지 처리를 실시하고 처리난백의 알레르기성의 변화를 평가하고자 passive cutaneous anaphylaxis(PCA) test, immunoblot, 생쥐모델에 의한 알레르기 유발시험을 실시하였다. PCA test결과 열처리($121^{\circ}C$, 30 min)한 난백은 처리하지 않은 난백에 비하여 그 항원성이 1/8 정도로 감소되었으며, NaOH처리군은 0.3%와 1%에서 각각 1/4, 1/8로 항원성의 감소를 보였다. 특히, NaOH처리 후 열처리(70oC, 15 min)를 추가적으로 복합처리한 난백의 경우, NaOH 0.3%(w/v)에서는 1/8 정도로, NaOH 1%(w/v)에서는 1/32 정도로 강력하게 항원성이 감소되었다. 계란 알레르기 환자의 IgE 항체를 이용하여 immunoblot을 실시한 결과 121oC로 열처리한 시료에서 난백의 주요 단백질 band가 흐려지는 것으로 나타났고 NaOH와 열로 복합처리한 난백의 경우에도 NaOH 0.1%(w/v) 이상에서 band들이 대부분 소실되는 것으로 나타났다. 생쥐모델에 의한 시험을 실시한 결과, 난백으로 유도한 전신알레르기 증상의 평균점수는 1.85이었으나 복합처리한 난백의 경우 그 점수가 0.20로 현격하게 감소되었다. 결론적으로, 상기 3종의 시험에서 공통적으로 가장 효과적인 난백의 알레르기성 저감화 방법은 복합처리(NaOH(1%, w/v) 및 열($70^{\circ}C$, 15min))임이 확인되었다.

• Archives of Pharmacal Research
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• 제20권2호
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• pp.122-127
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• 1997

The aqueous extract of Siegesbeckia pubescens (SPAE) inhibited compound 48/80-induced systemic anaphylaxis 100% with the dose of 1.0, 0.5 mg/g body weight (BW) at 1 h before or 5 min, 10 min after intraperitoneal injection of compound 48/80. The passive cutaneous anaphylaxis (PCA) reaction also inhibited to 78.5% by oral administration of SPAE(1.0 mg/g BW). When SPAE pretreated on mice at concentrations ranging from 0.0001 to 1.0 mg/g BW, the serum histamine levels were reduced in a dose-dependent manner. Moreover, SPAE ($100-800{\mu}g/ml) dose-dependently inhibited the histamine release from peritoneal mast cells (RPMC) by compound 48/80 $(5{\mu}g/ml)$. Analysis by microscopic appearance observation revealed that SPAE $(500{\mu}g/ml) stabilized the RPMC membrane. Therefore, these findings indicate that SPAE inhibits anaphylactic reactions through stabilization of mast cell membrane.

• Biomolecules & Therapeutics
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• 제12권2호
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• pp.129-132
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• 2004

During the screening program to discover antiatopic agents from herbal formulas, we investigated the inhibitory effect of Hwangryunhaedoktang (HT) on passive cutaneous anaphylaxis and oxazolone-induced mouse ear dermatitis. HT significantly inhibited PCA reaction in mice at doses of 50 and 200 mg/kg with inhibitory activity of 31 and 53%, respectively. HT at concentration of 0.05 and 0.1% inhibited ear swelling by 23 and 46% at 16 days in oxazolone-induced mouse ear dermatitis. Both HT with anti without human intestinal microflora showed potent inhibitory activity on the ${\beta}$-hexosaminidase release induced by IgE. Based on these findings, HT may be a usable agent for skin disorder contact dermatitis.

• 한방안이비인후피부과학회지
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• 제17권1호
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• pp.154-162
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• 2004

The flowers of Lonicera japonica Thunb. (Caprifoliaceae) have been used as a traditional effective drug in treating bacillary dysentery, purulence. However, the exact role of Lonicera japonica in allergic reaction has not been clarified yet. Immediate hypersensitivity, popularly known as allergy, is a major clinical problem in humans. It has been found that the histamine release from mast cells is an essential step in the pathological process of immediate hypersensitivity. In this study, the effect of aqueous extract of Lonicera japonica (AELJ) on allergic reaction was investigated. AELJ inhibited the compound 48／80-induced anaphylactic reactions and antigen-induced passive cutaneous anaphylaxis (PCA). AELJ in vitro exhibited a dose-dependent inhibition of degranulation in RPMC stimulated by compound 48／80. AELJ also suppressed the morphological changes and the increase of intracellular free calcium level induced by compound 48／80. These results suggest that inhibitory effect of AELJ on allergic reaction may be mediated through the decrease of intracellular free calcium levels, and AELJ importantly contributes to the treatment of anaphylaxis and may be useful for other allergic disease.

• Toxicological Research
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• 제8권1호
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• pp.139-143
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• 1992

Antigenicity tests-ASA(Active Systemic Anaphylaxis), PSA(Passive Systemic Anaphylaxis), PCA(Passive Cutaneous Anaphylaxis)-of CP-2(Green cross Co). were performed according to the established regulations of National Institute of Safety Research. The results were as follows. 1. No specic clinical signs related anaphylaxis were observed, therefore, it was considered that CP-2 had not antigenicity in guinea pigs and rabbits. 2. No blue spots were observed on the back of guinea pig in the PCA test` CP-2 related IgE was not produced.

• Biomolecules & Therapeutics
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• 제2권3호
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• pp.292-297
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• 1994

This study was conducted to investigate antigenic potential of DA-3030, a recombinant human granulocyte-colony stimulating factor, in guinea pigs and mice. In the active systemic anaphylaxis test, the guinea pigs sensitized with 1.25 or 12.5 $\mu\textrm{g}$/head of DA-3030 alone did not show any anaphylactic reaction. In the homologous passive cutaneous anaphylaxis reaction, anti-DA-3030 antibody was not detected in guinea pigs sensitized with 1.25 or 12.5 $\mu\textrm{g}$/head of DA-3030 alone. On the other hand, the guinea pigs sensitized with 12.5 $\mu\textrm{g}$/heed of DA-3030 incorporated in Freund's complete adjuvant(FCA) or 1 mg/head of ovalbumin incorporated in FCA showed anaphylactic reaction. Anti-DA-3030 antibody was also detected in those guinea pigs. In immunodiffusion test using the sera sensitized with DA-3030 incorporated in FCA, precipitating antibodies were detected only in the sera sensitized with DA-3030 or DA-3030 incorporated in FCA showed. In 24-hour heterologous PCA reaction with sera of C57BL/6 mice immunized with 1.25 or 12.5 $\mu\textrm{g}$/head of DA-3030 alone, none of the sera showed positive reaction. But sera of the animals immunized with 12.5 $\mu\textrm{g}$/head of DA-3030 incorporated in aluminum hydroxide gel(Alum) or 5 $\mu\textrm{g}$/head of ovalbumin incorporated in alum showed positive PCA reaction. DA-3030 did not cause anaphylactic shock or passive cutaneous anaphylaxis in guinea pigs and mice when given alone although DA-3030 incorporated in FCA or Alum induced anaphylactic shock and passive cutaneous anaphylaxis. From these results, it may be concluded the DA-3030 does not induce systemic allergic reaction when administered alone in its clinical use.

• 생명과학회지
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• 제21권10호
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• pp.1364-1369
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• 2011

제 1형 과민반응은 비만세포에 의해 매개되는 알러지 반응으로서, 예기치 못한 알러젠에 의해 비만세포가 활성화 되어 탈 과립 되면서 히스타민등과 같은 여러 가지 매개체들을 분비하면서 과도하게 면역반응을 유도하게 된다. 주박(Korean rice wine lees)은 청주, 약주 등의 술을 걸러내는 과정에서 생기는 양조 부산물로서, 최근들어 청주를 걸러내는 과정에서 생기는 술 지게미의 생리기능에 대한 연구가 활발하게 진행되고 있다. 본 연구에서는 주박의 ethyl acetate (EA) 추출물을 이용하여 비만세포에서의 탈과립 억제 효과를 측정하였다. 그 결과 주박 EA 추출물이 탈과립의 표지인 ${\beta}$-hexosaminidase의 양을 농도 의존적으로 억제하는 것을 관찰하였고, 이러한 탈과립억제 현상이 다양한 사이토카인 중 IL-3과 IL-13의 양을 감소시킴으로써 매개된다는 사실을 RT-PCR을 통해 확인하였다. 또한 쥐에서 passive cutaneous anaphylaxis (PCA)를 유도하여 EA를 경구투여 했을 때 anaphylactic 증상을 효과적으로 억제하는 것을 관찰하였다. 이러한 결과들은 주박 EA 추출물이 항 알러지 약물의 개발에 잠재적인 후보물질이 될 수 있음을 시사한다.

• Archives of Pharmacal Research
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• 제29권9호
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• pp.752-756
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• 2006

Artemisia princeps Pampanini, which is called Ssajuarissuk in Korean (SS-1), was fermented with lactic acid bacteria (LAB) and their passive cutaneous anaphylaxis reaction-inhibitory activity was investigated. Of these fermented agents, SS-1 extract fermented with Bifidobacterium infantis K-525 (F-SS-1) most effectively inhibited the release of ${\beta}$-hexosamindase from RBL-2H3 cells induced IgE. In IgE-induced RBL-2H3 cells, F-SS-1 inhibited proinflammatory cytokines IL-6 and $TNF-{\alpha}$ mRNA expression. Oral administration of SS-1 and F-SS-1 to mice inhibited passive cutaneous anaphylaxis (PCA) reaction induced by IgE and scratching behaviors induced by compound 48/80. The inhibitory activity of F-SS-1 against scratching behaviors was more effective than that of SS-1. These findings suggest that the fermentation of SS-1 with LAB can increase its antiallergic activity.

• Toxicological Research
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• 제11권1호
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• pp.157-159
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• 1995

A study on antigenicity of HRccine (formalin inactivated HFRS virus vaccine) was investigated in guinea pigs and mice. As a part of the safety evaluation of the HRccine, antigenicity tests were carried out according to the Estabilish Regulations of National Institute of Safety Research. In active systemic anaphylaxis (ASA) test no sign was detected when sensitized with up to 120 clinical dose and challenged with up to 1200 clinical dose in guinea pigs. In passive systemic anaphylaxis test guinea pigs showed no sign. In passive cutaneous anaphylaxis (PCA) test, HRccine specific IgE antibody was not detected when sensitized and challenged with up to 1200 clinical dose. Conclusively, there was no adverse antigenic potential at the clinical dose of 120 clinical dose alone and 120 clinical dose with Al(OH)3.