• Journal of Microbiology and Biotechnology
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• 제17권1호
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• pp.104-109
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• 2007

Lactic acid bacteria that accumulated ${\gamma}-aminobutyric$ acid (GABA) in culture medium were screened to identify strains with high GAB A-producing ability. One strain, MS, which was isolated from kimchi, showed the highest GABA-producing ability among the screened strains. MS was identified as Lactobacillus buchneri based on Gram-staining, metabolic characteristics, and 16S rDNA sequence determination, Optimum culture conditions for GABA production were determined: MRS broth containing 5% MSG, 1% NaCl, and 1% glucose, at an initial pH of 5.0, the incubation temperature at $30^{\circ}C$ for 36 h. Under these conditions, MS produced GABA at a concentration of 251 mM with a 94% GABA conversion rate. Moreover, culture extracts of Lb. buchneri MS partially or completely protected neuronal cells against neurotoxicantinduced cell death.

• Journal of Plant Biology
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• 제25권1호
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• pp.21-36
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• 1982

The activity of photosystem II in isolated chloroplast form the leaves of Sedum sarmentosum was measured. The photoreduction rate of DCPIP by photosystem II showed the circadian rhythm with a peak at near midday sample for a continuing fine day and at near afternoon between nidday and sunset sample for a continuing cloudy day in summer. The optimum light intensity of photoreduction by photosystem II in the chloroplast preparation was about 5~9$\times$$10^4$ lux. The saturated light intensity was over 9$\times$$10^1$lux. Photosystem II activity was inhibited by even the lowest concentration of lead. When Pi and ATP of the same concentration as Pb were added to the reaction mixture containing Tris buffer lacking of Pi prior to Pb incubation, photosystem II activity was protected from Pb-inhibiting effect by the pretreament of Pi and ATP. It was assumed that Pb inhibiiton was probably due to one, P-depriving by the precipitates of $Pb_3$ $($Pb_4$)_2$ in the reaction mixture and the other, partially Pb-combing with Pi groups of the active site of photosystem II.

• Steel and Composite Structures
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• 제1권4호
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• pp.427-440
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• 2001

The growing use of unprotected or partially protected steelwork in buildings has caused a lively debate regarding the safety of this form of construction. A good deal of recent research has indicated that steel members have a substantial inherent ability to resist fire so that additional fire protection can be either reduced or eliminated completely. A performance based philosophy also extends the study into the effect of structural continuity and the performance of the whole structural totality. As part of the structural system, thermal expansion during the heating phase or contraction during the cooling phase in most beams is likely to be restrained by adjacent parts of the whole system or sub-frame assembly due to compartmentation. This has not been properly addressed before. This paper describes an experimental programme in which unprotected steel beams were tested under load while it is restrained between two columns and additional horizontal restraints with particular concern on the effect of catenary action in the beams when subjected to large deflection at very high temperature. This paper also presents a three-dimensional mathematical modelling, based on the finite element method, of the series of fire tests on the part-frame. The complete analysis starts with an evaluation of temperature distribution in the structure at various time levels. It is followed by a detail 3-D finite element analysis on its structural response as a result of the changing temperature distribution. The principal part of the analysis makes use of an existing finite element package FEAST. The effect of columns being fire-protected and the beam being axially restrained has been modelled adequately in terms of their thermal and structural responses. The consequence of the beam being restrained is that the axial force in the restrained beam starts as a compression, which increases gradually up to a point when the material has deteriorated to such a level that the beam deflects excessively. The axial compression force drops rapidly and changes into a tension force leading to a catenary action, which slows down the beam deflection from running away. Design engineers will be benefited with the consideration of the catenary action.

• 생물환경조절학회지
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• 제28권4호
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• pp.420-428
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• 2019

본 연구에서는 고설 딸기 관부(크라운부) 난방시스템을 전기 온수 보일러, 축열조, 순환 펌프, 관부난방 배관(백색 연질 PE관, 관경 16mm) 및 온도 제어반으로 구성하였다. 관부(크라운부) 난방의 경우 난방 배관을 딸기 관부에 최대한 밀착될 수 있도록 설치하고 배관 위치를 원예용 고정핀으로 고정하였다. 또한 관부 난방시스템의 에너지 효율을 증진하기 위해 축열조 온수 온도를 $20{\sim}23^{\circ}C$, 관부 온도를 $13{\sim}15^{\circ}C$로 관리하였다. 관부난방은 전기 온수보일러를 이용하여 $20{\sim}23^{\circ}C$의 온수를 축열조에 저장하고 순환펌프를 제어하기 위한 온도 센서를 딸기의 관부에 최대한 근접하여 설치하고 온도를 감지함으로써 관부(크라운부)를 집중적으로 난방하는 방식이다. 시험 온실의 난방 처리는 공간 난방 $4^{\circ}C$ + 관부난방(처리 1), 공간 난방 $8^{\circ}C$ (대조구), 공간 난방 $6^{\circ}C$ + 관부난방(처리 2)로 처리하였다. 각 난방처리는 온실 1동에 딸기를 980주를 심었으며, 재배방법은 표준재배법에 준해서 재배하였다. 난방 에너지 소비에 대한 비교시험은 2017년 11월 8일부터 2018년 3월 30일까지 수행되었다. 소비된 누적 전력량은 등유 사용량으로 환산하였고, 등유 소비량은 공간난방 $8^{\circ}C$(대조구)의 경우 1,320L(100%), 공간난방 $4^{\circ}C$ + 관부난방의 경우 928L(70.3%), 공간난방 $6^{\circ}C$ + 관부난방의 경우 1,161L (88%)로 계측되었다. 공간난방 $4^{\circ}C$ + 관부난방(처리 1) 및 공간난방 $6^{\circ}C$ + 관부난방(처리 2)은 $8^{\circ}C$ 공간난방(대조구)에 비해 생육 저하, 수확시기의 지연 등이 없이 비슷하게 딸기 수확이 가능하였으며, 29.7% 및 12%의 난방 에너지가 절감되는 것으로 분석되었다.

• Molecular & Cellular Toxicology
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• 제4권1호
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• pp.16-21
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• 2008

Cell death of trophoblast, particularly by abnormal release of physiological nitric oxide (NO) has been known to be a causative factor of pre-eclampsia. In the present study, effects of intracellular calcium increase enhancing the activity of NO synthases (neuronal NO synthase, nNOS in this trophoblast cells) on the cell death were examined in a human placental full-term cell line (HT-1). Furthermore, we analyzed the possible mechanisms underlying the augmentation of $Ca^{++}$-mediated NOS activity mediated by protein kinases like PKC, PKA, or CaM-KII. In experiments for cell toxicity, a calcium ionophore (ionomycin $10{\mu}M$) enhanced cell death confirmed by MTT assay, and increased significantly nNOS activity determined with a hemoglobin oxidation assay. This cell death was partially protected by pre-treatment of 7-nitroindazole (7-NI, $10{\mu}M$ and $100{\mu}M$), a nNOS-specific inhibitor. Additionally, $Ca^{++}$-ionophore -induced increase of nNOS activity also was partially normalized by pre-treatment of specific inhibitors of protein kinases, PKC, PKA or CaM-KII. Therefore, we suggest that an increase of calcium influx, leading to the activation of nNOS activity, which in turn may result in the death of trophoblast cells by involvement of signaling mechanisms of protein kinases.

• 한국안광학회지
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• 제12권3호
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• pp.97-103
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• 2007

본 연구는 UV-A 노출에 의해 유발된 안구 내에 존재하는 항산화효소인 catalase와 superoxide dismutase(SOD)의 변성을 차단할 수 있는 안경렌즈의 적절한 UV-A 차단율을 알아보기 위해 수행하였다. Catalase와 SOD를 1, 3, 6, 24, 96시간 동안 365 nm의 UV-A에 노출시켜 노출시간에 따른 단백질의 변성 정도를 아크릴아미드 겔 전기영동법으로 확인하였다. 또한, 20, 50, 80 및 99% UV 차단 효과를 가진 안경렌즈로 UV-A를 차단하였을 때 catalase와 SOD의 변성이 억제될 수 있는 지를 알아보았다. Catalase는 3시간 이상의 UV-A 노출에 의해 변성되기 시작하였다. 그러나, SOD는 6시간 이상 노출시에 변성이 되기 시작하였다. 99% 차단렌즈에 의해 3시간 동안의 UV-A 노출에 의한 catalase 변성을 완전히 억제할 수 있었다. 그러나 노출시간이 3시간보다 길어지거나 차단율이 99%보다 낮은 렌즈를 사용한 경우, 어느 정도까지는 변성 억제작용은 있었으나 완벽하게 변성을 억제하지는 못하였다. 비록 50% 차단율을 가진 렌즈는 UV-A에 대한 SOD의 변성에 대해 부분적인 억제 작용을 나타냈으나, 80% 및 99%의 차단율을 가진 안경렌즈는 UV-A에 의한 SOD의 변성을 완전히 억제하였다.

• 한국안전학회지
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• 제32권1호
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• pp.66-74
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• 2017

Masonry walls which are commonly used for partitions in low-rise reinforced concrete (RC) structures, can be easily exposed to high risks under strong earthquakes. Since the strength degradations cannot be protected under the ground motions, their applications cannot be recommended for building structures which are designed to possess high seismic performances. However, masonry-infilled walls are typically considered as non-structural elements in evaluating the seismic performance of building structures. In order to figure out this problem, this study performed experiments using two specimens-only RC frame and RC frame infilled with masonry walls- under static loading. Also, the study established analytical models representing fully infilled frames and bare frame, and compared their structural behavior with test results. In addition, analytical model representing partially infilled frames was established and analyzed. Test results indicated that strength and energy dissipating capacity were increased for IW-RN(fully infilled frames) compared to the NW(bare frame). The nonlinear static analysis of the three specimens was also conducted using the inelastic plastic hinge frame element and diagonal strut models, and the analytical results successfully simulated the nonlinear behaviour of the specimens in accordance with the test results.

• 한국염색가공학회지
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• 제22권4호
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• pp.349-355
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• 2010

To investigate the half-degumming of raw silk, the degumming was carried out with soap or sodium carbonate or sodium bicarbonate and some mixed agents at $80^{\circ}C$ for 15 and 30 minutes. With soap of 20~25%o.w.f. degumming losses were about 7~12%. Degumming losses with sodium carbonate of 2~5%o.w.f. were about 6~15%. And about 6~12% wright losses were observed in the degumming with sodium bicarbonate of 20~30%o.w.f. In the degumming with the mixed degumming agents of soap and sodium carbonate, degumming losses were 9~15% with 5%o.w.f. soap and 2~5%o.w.f. sodium carbonate. Sodium carbonate was more effective on the removal of sericin from raw silk than soap. During drying generally half degummed silk yarns stuck together to result in harsh and hard lumps by the adhesion and solidification of the residue sericin of partially degummed silk. Sodium hydrosulfite in degumming agent effectively protected the adhesion of half degummed silk yarns.

• 대한한의학회지
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• 제30권3호
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• pp.86-97
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• 2009

Objectives : This study was aimed to investigate the effects of GamiSamgieum (SGMX) on hyperlipidemia using an animal model. Additionally, the underlying mechanisms were investigated by determination of gene expressions related with lipid metabolism. Methods : Forty mice were selected for use in this experiment, and then divided into five groups; Naive, Induced, SGMX 100, SGMX 200, and Lovastatin group as positive control with 8 mice each, and their blood was collected for analysis of blood and serum parameters. Results : Administration of SGMX significantly inhibited the increase of liver weight and the macrovacuolar cytoplasmic alterations in histopathologic finding. SGMX administration significantly protected the liver from pathologic elevation of AST and ALT and from lipid peroxidation. SGMX administration significantly lowered total cholesterol levels and TG, and partially upregulated the gene expression of LCAT, CYP7A1 and LDL-R receptor. Conclusion : SGMX is suggested to possess hypolipidemic effect, and thus could be a potential candidate for herbal hypolipidemic via further studies.

• Biomolecules & Therapeutics
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• 제21권3호
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• pp.210-215
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• 2013

Ionizing radiation can induce cellular oxidative stress through the generation of reactive oxygen species, resulting in cell damage and cell death. The aim of this study was to determine whether the antioxidant effects of the flavonoid fisetin (3,7,3',4'-tetrahydroxyflavone) included the radioprotection of cells exposed to ${\gamma}$-irradiation. Fisetin reduced the levels of intracellular reactive oxygen species generated by ${\gamma}$-irradiation and thereby protected cells against ${\gamma}$-irradiation-induced membrane lipid peroxidation, DNA damage, and protein carbonylation. In addition, fisetin maintained the viability of irradiated cells by partially inhibiting ${\gamma}$-irradiation-induced apoptosis and restoring mitochondrial membrane potential. These effects suggest that the cellular protective effects of fisetin against ${\gamma}$-irradiation are mainly due to its inhibition of reactive oxygen species generation.